Helicobacter pylori cagE 유전자 다형성이 위상피세포주에서 Interleukin-8 유도능에 미치는 영향

이수민,이학성,이인석,조유경,한혜원,오정환,박재명,최명규,정인식,Lee Su-Min,Lee Hak-Sung,Lee In-Suk,Co Yu-Kyung,Han Hae-Won,Oh Jung-Hwan,Park Jae-Myung,Choi Myung-Gyu,Chung In-Sik 한국생명과학회 2004 생명과학회지 Vol.14 No.6

Helicobacter pylori infection is highly prevalent, as high as 2/3 of whole population infected, in Korea. H. pylori infection initiates inflammation by induction of interleukin-8 through type IV secretion of CagA. It was recently suggested that induction failure of IL-8 is not associated with defect in cag PAI but associated with cagE locus diversity. This study was designed to investigate ability of 11-8 in-duction according to sequence variation within the cagE gene, cagA TP motifs and vacA m-types in vitro study using AGS cell-line, and to evaluate its association with different clinical outcome. Seventy-four H. pylori stains were isolated from 23 patients with gastric cancer (Ca), 24 subjects with gastritis (G) and 27 patients with duodenal ulcer (Du) in Kangnam St. Mary's Hospital, Seoul, Korea. cagE gene diversity was confirmed by the PCR-RFLP methods with MboI/NlaIII and tyrosine phosphate motifs (TPMs) of cagA was determined TPM-A and C by using DdeI/Tsp5091 restriction enzyme and TPM-B was determend by Real time PCR the method of Owen et al. and IL-8 was measured by ELISA assay. IL-8 activity was positively detected in 59 among 74 strains $(79.7\%)$. IL-8 secretion was significantly increased in MboI A and MboI B type compared to MboI C type and in MboI/NlaIII A-C and B-C type than C-C type. 1L-8 activity was not associated with either the number or composition of cagA tyrosine phosphorylation motifs and vacA m-type. There was no significant difference in IL-8 activity among patient groups. cagE gene diversity is thought to be mainly associated with the induction of IL-8 in H. pylori infection. 한국에서 분리한 Helicobacter pylori 균주가 위암 세포주에서 IL-8를 유도하는 유도능을 관찰하고자 하였으며, 특히 4형 분비구조의 주요 구성체인 cagE 유전자의 다형성에 따라 IL-8 유도능의 차이 및 각 질환에서 분리한 균주에서의 차이가 있는지 검토하고자 하였다. 강남성모병원 내시경검사를 받은 환자들 중 H. pylori 감염 양성으로 확인된 위암 23명 $(31.0\%)$, 위염 24명$(32.6\%)$, 십이지장궤양27명$(36.4\%)$ 총 74명을 대상으로 하여 H. pylori를 분리한 후 PCR-RFLP방법을 이용하여 cagE 다형성을 확인하였으며, PCR 및 Real-time PCR을 이용하여 ragA TPMs를 결정하였다, 또한 분리된 균주를 위암세포주에 감염시켜 상등액을 취하여 ELISA방법으로 IL-8을 정량화 하였다. 전체 74개의 분리 균주 중 59개의 균주$(79.7\%)$에서 IL-8 유도능이 있는 것으로 확인되었으며, cagE 다형성 중에서 가장 높은 빈도로 관찰되는 형태는 MboI/NlaIII B-C, A-C 및 C-C 타입 이었으며, 이중 A-C와 B-C 형태가 C-C 형태에 비해 유의적으로 높은 IL-8 유도능을 나타냈다. 그러나 cagA TPMs의 분류 및 vacA m-아형 분류와 IL-8 유도능과는 연관성이 없었다. 비록 본 연구에서 cagE 다형성과 IL-8 유도능과 아주 밀접한 연관성이 나타나지는 않았지만 vacA m-아형이나 cagA TPMs 보다는 cagE 다형성이 IL-8 유도능과 연관성이 클 것으로 생각된다.

박막형 크로멜-알루멜 다중접합 열전변환기

정인식,김진섭,이정희,이종현,신장규,박세일,권성원,Jung, In-Sik,Kim, Jin-Sup,Lee, Jung-Hee,Lee, Jong-Hyun,Shin, Jang-Kyoo,Park, Se-Il,Kwon, Sung-Won 대한전자공학회 1999 電子工學會論文誌, D Vol.d36 No.9

박막형 다중접합 열전변환기의 시간에 따른 출력 전압 변화를 감소시키기 위해 벌크의 저항온도계수가 매우 적은 EVANOHM-S 합금을 박막 히터재료로 사용하였고, 또한 Seebeck 계수차이가 비교적 작은 크로멜-알루멜 열전쌍을 박막 열전퇴(thermopile)의 열전요소로 하였다. EVANOHM-S 박막 히터의 저항온도계수는 약 $1.4 {\times} 10^4/^{\circ}C$ 였고, 크로멜-알루멜 박막 열전쌍의 Seebeck 계수차이는 약 $38 {\mu}V/K$였다. 열전변환기의 출력 전압 변화는 공기중에서 처음 120초 동안 약 0.06%였고, 약 5분간이상 히터의 예열후 출력전압 변화는 현저히 감소하였다. 10 Hz ~ 10 kHz의 주파수 범위에서 열전변환기의 교류-직류 전압 및 전류 변환 오차범위는 각각 ${\pm}$1.6 ppm 및 ${\pm}$0.7 ppm이었고, 10Hz 이하 또는 10 kHz 이상의 주파수에서는 교류-직류 변환오차가 크게 증가하였다. For the purpose of reducing the output voltage fluctuation of thin film multijunction thermal converter, EVANOHM alloy-S and chromel-alumel thermocouple were used as a thin film heater material and as a thermoelement of thrmopile, respectively. The temperature coefficient of the resistance of thin film EVANOHM alloy-S heater was about $1.4 {\times} 10^4/^{\circ}C$, which is very small compared to other materials, and thin film chromel-alumel thermocouple showed relatively small difference of the Seebeck coefficients about $38 {\mu}V/K$. The output voltage fluctuation of the thermal converter was about 0.06% for the initial 120 seconds in air and decreased considerably after preheating for 5 minutes or more. The respective AC-DC voltage and current transfer error ranges of the thermal converter were about ${\pm}$1.6 ppm and ${\pm}$0.7 ppm in the frequency range from 10Hz to 10 kHz and increased remarkably below 10 Hz or above 10 kHz.

5'-Untranslated Region에 존재하는 Iron Responsive Element에 의한 Ferritin 합성조절

정인식,이중림,김해영,Chung, In-Sik,Lee, Jung-Lim,Kim, Hae-Yeong 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.33 No.2

철의 대사과정에 관여하는 ferritin 단백질의 발현은 ferritin transcript의 5'-untranslated region에 위치한 iron-responsive element (IRE)와 철 농도 조절 단백질의 결합에 의해 조절된다. 이러한 ferritin의 생성에 관여하는 구조적인 요소를 밝히기 위해, RNA 이차구조인 IRE의 bulge 부분을 다른 염기로 변환시켜 철 농도 조절단백질에 의한 RNA 결합력과 ferritin 단백질의 생성의 저해정도를 비교 측정하였다. 측정된 결과로부터 IRE의 bulge 부분의 시토신 염기배열만이 RNA 이차구조의 형성에 중요한 작용을 하여 ferritin 합성을 조절할 수 있는 것을 보였다. The expression of ferritin involved in iron metabolism is regulated at the translational level by the interaction of iron regulatory protein with iron-responsive element(IRE) in the 5'-untranslated region of ferritin transcript. To identify the role of structural element utilized for translational regulation of ferritin, we studied the effects of mutations in the ferritin IRE by measuring IRP binding activity and translational activity. Our data suggest that the cytosine at bulged position of IRE within ferritin is important for the formation of RNA secondary structure involved in translational regulation.

막여과 기반의 직결형 정수처리시설의 적용에 관한 연구

서인석 ( In-seok Seo ),김성수 ( Seung-su Kim ),한현 ( Hyun Han ),박상광 ( Sang-kwang Park ),정인식 ( In-sik Jung ) 한국수처리학회 2022 한국수처리학회지 Vol.30 No.5

In this study, direct-coupled water treatment facilities with the capacity of 500 ㎥/day were installed and operated in Indonesia and Cambodia, respectively. In Indonesia, raw water was taken from the reservoir, and the water treatment plant for the process consisting of UF-AOP-GAC-Chlorination was installed horizontally. In Cambodia, groundwater was used as raw water, and the water treatment plant for the process consisting of UF-GAC-Chlorination was installed vertically. All water treatment plants were operated stably, and the produced water was suitable for drinking in terms of water quality standards. In the detailed evaluation of the unit process, the TMP of the UF process was stable at 0.35 ~ 0.4 kgf/㎠ when the raw water was groundwater with low turbidity, and chemical cleaning was unnecessary during the operation period of about 10 months; Meanwhile, in Indonesia where the turbidity of raw water is relatively high, the TMP was varying between 0.4 ~ 1.0 kgf/㎠ and chemical cleaning was required once a month. In addition, the differential pressure of GAC adsorber was maintained at a low level of 0.1 kgf/㎠ or less. Also, the electricity consumption was evaluated to be in the range from 0.23 to 0.26 kWh/㎥ for the operation of the Indonesian plant and 0.176 kWh/㎥ in Cambodia. The electricity consumption was significantly affected by the increase of TMP due to the turbidity of raw water, and the operating pressure needed to be minimized.

역류식도염 환자에서 Helicobacter pylori 감염과 Gastrin, Pepsinogen의 관계 -역류식도염과 H. pylori 감염, 위축위염의 관계-

권정현 ( Jung Hyun Kwon ),정인식 ( In Sik Chung ),손혜숙 ( Hye Suk Son ),박재명 ( Jae Myung Park ),조유경 ( Yu Kyung Cho ),이인석 ( In Seok Lee ),김상우 ( Sang Woo Kim ),최명규 ( Myung Gyu Choi ) 대한소화기학회 2008 대한소화기학회지 Vol.51 No.3

목적: H. pylori 감염은 위축 위염의 중요한 원인이 되며, 혈청 gastrin과 pepsinogen (PG) 분비에도 영향을 미친다. 위식도역류질환 환자에서 H. pylori의 빈도가 낮다는 보고들이 있으나 위식도역류질환의 발생과 H. pylori 감염의 관계는 아직도 분명치 않다. 이번 연구에서는 미란 식도염(erosive reflux esophagitis, ERD)의 원인인자인 위산 분비와 관련이 있는 H. pylori 감염과 gastrin, PG I, II 및 I/II 비와의 관계를 알아보고자 하였다. 대상 및 방법: 환자 2006년 3월부터 10월까지 강남성모병원에서 한 명의 내시경 전문의가 상부위 장관 내시경 검사로 ERD로 진단한 환자를 대상으로 하였고 나이와 성을 대응시켜 대조군을 선정하였다. 두 군 모두 내시경 검사에서 궤양 등의 다른 위장관 질환이 있거나 제균 치료를 받은 자는 제외하였다. 음주와 흡연 유무, 신체질량 지수(BMI)를 조사하였고 H. pylori 감염, 혈청 PG I, II (라텍 스응집면역비탁법)와 gastrin (방사면역측정법)을 검사하였다. 결과 총 45명의 ERD 환자군과 66명의 대조군을 비교하였다. H. pylori 감염은 ERD군에서 대조군보다 낮았다(11.1%vs. 43.9%, p<0.05). PG I/II 비는 ERD군에서 대조군보다 높았다(7.0±3.1 vs. 5.3±2.6, p<0.05). PG II와 gastrin은 ERD군에서 대조군 보다 낮았다(p<0.05), BMI는 ERD군이 대조군보다 높았다(24.46 kg/m2 vs. 23.08 kg/m2, p<0.05). 결론: 한 국인에서 ERD 환자군은 대조군보다 H. pylori 감염률이 낮고, PG I/II 비가 증가되어 있어 역류식도염은 위점막의 위축이 더 적은 환자에서 잘 생기는 것으로 생각한다. PG I/II비는 H. pylori 감염 검사와 함께, ERD 환자군에서 동반되어 있는 위축 위염의 형태나 정도, 위산 분비 정도를 예측해 볼수 있는 인자로서 유용성이 있다고 생각한다. Background/Aims: Helicobacter pylori (H. pylori) infection is known as a major cause of atrophic gastritis and is associated with serum gastrin, pepsinogen, and gastric acid secretion. There is still a controversial association between gastroesophageal reflux disease and H. pylori infection. This study was designed to investigate the relationship among serum gastrin, pepsinogen, and H. pylori infection in the erosive reflux esophagitis (ERD) patients. Methods: Patients who were diagnosed as ERD by one gastroenterologist at the Kangnam St. Mary`s hospital were prospectively enrolled. The persons without ERD in the control group were matched for age and sex. We examined the gastrin, pepsinogen I (PG I), PG II, PG I/II ratio, and H. pylori infection. Results: Forty five patients were enrolled in ERD group and 66 persons in control group. The H. pylori infection rate in ERD group was lower than that in the control group (11.1% vs. 43.9%, p<0.001). PG I/II ratio in ERD group was higher than that in the control group (7.0±3.1 vs. 5.3±2.6, p=0.003). The PG II (p=0.016) and gastrin (p=0.029) in ERD group were lower than those in the control group. BMI in ERD group was higher than that in the control group (24.5 vs. 23.1 kg/m2, p=0.013). Conclusions: The H. pylori infection rate in ERD group was lower and PG I/II ratio was higher than that in the control group. Reflux esophagitis is thought to be reversely associated with the atrophy of gastric mucosa. (Korean J Gastroenterol 2008;51:159-166)

쯔쯔가무시병 환자에서의 장내 단백 상실

민준기(Jun Ki Min),정우철(Woo Chul Jung),백기현(Gi Hyun Baek),김양리(Yang Ree Kim),오수혁(Soo Hyuk Oh),강문원(Moon Won kang),정인식(In Sik Chung),양우진(Woo Jin Yang),김성훈(Sung Hun Kim) 대한내과학회 1996 대한내과학회지 Vol.51 No.4

N/A Objectives: Tsutsugamushi disease is an acute, febrile illness of humans that is caused by Rickettsia tsutsugamushi. Hypoalbuminemia was reported in some cases of tsutsugamushi disease, but its frequcncy and etiology were not clarified. Therefore we prospectively evaluated fourteen cases of Tsutsugamushi disease patients to idendify intestinal protein loss as a cause of hypoalbuminemia and to assess the value of 99mTc-HSA abdominal scintigraphy for detecting intestinal protein loss. Methods: From November 1994 to December 1994, in fourteen patients with tsutsugamushi disease, serum albumin level and fecal a-1-antitrysin excretion were measured at admission. Anterior abdominal images were obtained at 2, 4, and 24hours after intravenous injection of 30 mCi of Tc-Human serum albumin. Results: 1) The age distribution was mainly in the forties, and fifties. Geographically, 10 cases in the northern part of Kyung-Ki Do and 4 cases in Seoul were occurred. 2) Laboratory findings showed hypoalbuminemia in 5 cases(35.7%), elevated AST and ALT in 11 cases (78.6%), prolonged prothrombin time in 1 cases (7.1%), and proteinuria(30mg/dl) in 9 cases(64.3%). 3) Fecal a-1-antitrypsin concentration was measured in 10 cases of 14 patients with tsutsugamushi disease, of which 8 cases revealed abnormally elevated concentration of fecal a-l-antitrypsin(2.6mg/g dry weight). 4) 99mTc-HSA scintigraphy was positive in 11 of 1478.696) patients with tsutsugamushi disease. 5) In patients with positive 99mTc-HSA scintigraphy, protein loss sites were small bowel(6/11, 54.5%), descending colon(3/14, 27.3%), ascending colon(2/11, 18.1%). Conclusion: Intestinal protein loss was idendified in some patients with tsutsugamushi disease by the measurement of fecal a-1-antitrpysin concentration. 99mTc-HSA scintigraphy is easy and readily available study for detecting intestinal protein loss sites. The comparison of the severity of intestinal protein loss before and after treatment is recommended in patients with tsutsugarnushi disease who have abnormal intestinal protein loss.

위궤양환자에서 Rebamipide의 자유산소유리기제거에 의한 궤양치료효과

박수헌(Soo Heon Park),정운태(Woon Tae Jung),서정한(Jung Han Suh),정명희(Myung Hee Chung),정인식(In Sik Chung),이용찬(Yong Chan Lee),박인서(In Suh Park),송근암(Guen Am Song),양웅석(Ung Suk Yang),유종선(Jong Sun Rew),윤종만(Chong Mann 대한소화기학회 1997 대한소화기학회지 Vol.29 No.5

N/A Background/Aims: Helicobacter pylori has been shown to be an important pathogen of gastric disorder including gastritis and gastric ulcer. One of the potential toxic factors involving gastric mucosal cell injury is reactive oxidants, which are released from activated neutrophils. Rebamipide had been reported to promote mucus synthesis, mucosal prostaglandin content, and rapid ulcer healing, It has recently been reported to inhibit the production of oxygen derived free radicals from stimulated neutrophils. We conducted this study to evaluate the effects of rebamipide on gastric ulcer healing and reactive oxygen metabolite production. Methods: In this multicenter study conducted in Korea, combination treatment of rebamipide and ranitidine was compared with ranitidine alone in 108 patients with gastric ulcer patients. Pre- and post-treatment endoscopic findings and concentration of reactive oxygen metabolite produced were used to evaluate efficacy. The efficacy of rebamipide and ranitidine versus ranitidine was evaluated using endoscopy results, and tissue lipid peroxides by thiobarbituric acid and myeloperoxide concentration. Results: The percentage of ulcer size reduction was significantly higher with rebamipide and ranitidine group than with placebo and ranitidine group(size reduction: 71.5% vs 58.3% P=0.04556). The myeloperoxidase activities at ulcer margin in rebamipide and ranitidine group patients were 96.7+-13.4 unit/mg protein in baseline and 53.8+9.9 unit/mg protein in follow up (P=0.0065). The myeloperoxidase activities at ulcer margin in placebo and ranitidine group patients were 90.2+9.1 unit/mg protein in baseline and 63.1+6.7 unit/mg protein in follow up (P=0.0668). The thiobarbituric acid activities at ulcer margin in rebamipide and ranitidine group patients were 34.7+5.3 nmol of MDA/mg protein in baseline and 24.1W3.1 nmol of MDA/mg protein in follow up. The thiobarbituric acid activities at ulcer margin in placebo and ranitidine group patients were 14.8+1.5 nmol of MDA/mg protein in baseline and 24.3+2.7 nmol of MDA/mg protein in follow up (P=0.0059). The thiobarbituric acid activities at ulcer margin in H. pylori positive and negative patients were 28.1+2.1 nmol of MDA/mg protein and 25.3+4.7 nmol of MDA/mg protein, respectively. The myeloperoxidase activities at ulcer margin in H. pylori positive and negative patients were 79.9+5.3 unit/mg protein and 40.4+25.3 unit/mg protein, respectively (P=0.0001). The cumulative relapse rate was examined starting at 24 week after ulcer treatment. The rate was lower in rebamipide and ranitidine group patients, but insignificantly(19.2% vs 27.3%, P=0.0857). Conclusions: These results suggest that rebamipide promote gastric ulcer healing by decreasing reactive oxygen metabolite production in patients with gastric ulcer. (Korean J Gastroenterol 1997;29:569-578)

성서폐수처리장 방류수 재이용을 위한 Pilot plant 최적 운전조건 확립

정인식 ( In Sik Jung ),김현정 ( Hyeon Jeong Kim ),장지웅 ( Ji Ung Jang ),신용일 ( Yong Il Shin ),박병주 ( Byung Joo Park ),차춘근 ( Chun Geun Cha ) 한국물환경학회(구 한국수질보전학회) 2011 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2011 No.-

모바일 통합 SNS 게이트웨이의 웹 서비스 어댑터 설계 및 구현

정인식(Jung In Sik),김현우(Kim Hyeon Woo),주홍택(Ju Hong Teak) 한국통신학회 2013 한국통신학회 학술대회논문집 Vol.2013 No.6

구연발표 : Secretin 의 위산분비 억제기전

정인식(In Sik Jung),선희식(Hee Sik Sun),김부성(Boo Sung Kim) 대한소화기학회 1988 대한소화기학회 추계학술대회 Vol.1988 No.-