만성 실질성 간질환 환자 혈청에서 Basic Fibroblast Growth Factor 및 Angiogenin 농도의 변동

백인규(In Kyu Paik),이숭환(Soong Hwan Lee),조윤주(Yun Ju Cho),이성희(Sung Hee Lee),김홍주(Hong Ju Kim),남승우(Seung Woo Nam),고희관(Hee Kwan Koh),이창범(Chang Beom Lee),박동일(Dong Il Park),조영중(Yong Jung Cho) 대한소화기학회 1996 대한소화기학회지 Vol.28 No.6

N/A Background/Aims: Liver fibrosis by the progression of the chronic process of the liver disease induces deformed microcirculations of the hepatic lobules and this eventually resulted in portal hypertension. Angiogenic stimulant factors are physiologically activated in order to repair the tissue damage. Overexpression of angiogenic factors, however, can stimulate neovascularization as in a formation of the hypervascular tumor that liberates uncontrolled overgrowing of the tumor cells. To elucidate the dynamic changes of the serum concentration of basic fibroblast growth factor(bFGF) and angiogenin in chronic liver diseases, this study is intended to employ an ELISA out of pathologically proven patients. Methods: Sera taken out of the 44 patients with chronic persistent hepatitis(5 cases), chronic active hepatitis(6 cases), liver cirrhosis(19 cases) and hepatocellular carcinoma(14 cases) were tested for bFGF and angiogenin employing Quantikine' ELISA Kits (R & D Systems Inc. Minneapolis, MN) that pathological diagnosis was proven )ater. The statistical analysis was evaluated by students t-test. Results: Serum mean value and standar<I error of bFGF concentration(pg/ml) was 11.851.98 in 19 cases of liver cirrhosis, 9.86+2.35 in 14 cases of hepatocellular carcinoma, 9.48+4.57 in 6 cases of chronic active hepatitis, and 8.29+2.63 in 5 cases of chronic persistent hepatitis. Mean value and standard error of angiogenin concentration (ng/ml) of the sera was 238.92+50.95 in 5 cases of chronic persistent hepatitis, 184.47+12.75 in 6 cases of chronic active bepatitis, 131.36+10.99 in 19 cases of liver cirrhosis, and 211.03+19.08 in 14 cases of hepatocellular carcinoma, respectively. Serum angiogenin concentration in liver cirrhosis was significantly lower than that in chronic persistent hepatitis(p=0.0033(I), and than that in chronic active hepatitis(p=0.018673). Angiogenin concentration in hepatocellular carcinoma was very significantly elevated, when compared with the level of the liver cirrhosis investigated (p=0.000569). Conclusions: These data suppoit that persistent inflammatory insults in chronic hepatitis were compensated by the elevation of angiogenin, but complete fibrosis as in liver cirrhosis showed the depressed level of angiogenin. Again, emerging of the hepatocellular carcinoma is accompanying with the elevated stitnuli of angiogenin for the neovascularization. In contrast, bFGF in this study was statistically not significant but may be related with fibrosis and reconstruction of microvascular system accompanying with progression of chronic parenchymal liver diseases to liver cirrhosis.(Korean J Gastroenterol 1996; 28:806-814)

만성간질환에서의 혈청 procollagen 1/3 propeptides 농도비 측정의 의의

유용걸(Yong Keol Yoo),이숭환(Soong Hwan Lee),김병훈(Byoung Hun Kim),조영중(Young Jung Cho),백인규(In Kyu Paik),유병무(Byeong Moo Yoo),이오영(Oh Young Lee),한동수(Dong Soo Han),손주현(Joo Hyun Sohn),최호순(Ho Soon Choi),이동후(Dong Ho 대한소화기학회 1996 대한소화기학회지 Vol.28 No.6

N/A Background/Aims: Most of Ihe chronic liver diseases of variable etiologies are accompanied by a pathobiochemical reaction So-called fibrosis. Liver fibrosis was recently recognized as all the complexity of dynamic changes of the hepatic extracellular matrix(ECM). And ECM consists of four major components: collagen, glycoprotein such as fibronectin and laminin, proteoglycan, and elastin. Among these, collagen I and III are the representatives of disproportion of synthesis and degradation in conjunction with fibrogenesis. To elucidate the clinical significance of procollagen propeptide I(PICP) and III(PIIINP) measurements among the sera out of patients with chronic liver diseases, radioimmunoassay 4vas employed in this investigation. Methods: Sera tested were obtained from pathologically-proven 54 patients; 4 cases of fatty liver, 11 cases of chronic persistent hepatitis, 13 cases df chronic active hepatitis, 15 cases of liver cirrhosis and 11 cases of hepatocellular carcinoma. All:the patients except 4 cases of fatty liver were shown positivity of HBsAg. PICP and PIIINP radioimmunoassay kits(Farmos Diagnostica, Oulunsalo, Finland)were purchased for this study. ResOlts: PICP concentration (mean+standard error) was highest in liver cirrhosis (280.06>39,83 pg/L). PICP in liver cirrhosis was significantly elevated in cornparison to that (109.30+14.58) in fatty:liver(p=0.045), that (122.22>10.26) in chronic persistent hepatitis (p=0.003), and that (158.59>21.58) in chronic active hepatitis(p=0.016), PICP in hepatocellular carcinoma was decreased to 1'94.05+ 29.89. Similarly, PIIINP concentration revealed highest level in liver cirrhosis(17.29+3.58 pg/L). This value was significantly higher than that of chronic persistent hepatitis(5.12+0.59)(p=0.008). Contrarily to these results, concentration ratio of PICP/PIIINP was high in fatty liver (29.27>6.29) and chronic persistent hepatitis (25.04+2.00), These value were statistically higher than that of chronic active hepatitis (17.632.67) (p=0.043). PICP/PIIINP ratio in liver cirrhosis (21.58+3.29) showed no statistical difference. Conclusions: On the basis of these data, it is suggested that the elevation of PICP and PIIINP in the sera of the patients with chronic liver diseases means emerging the liver cirrhosis. And the ratio of these markers can be helpful in differential diagnosis of chronic liver diseases. (Korean J Gastroenterol 1996; 28:815-823)

말기 신부전증 환자에서 혈액 투석중 혈장 칼륨에 대한 Nive Beta - adrenergic Blockade ( carteolol ) 와 ACE Inhibitor ( captopril ) 의 영향

이재웅(Jae Ung Lee),이오영(Oh Young Lee),양석철(Suck Chul Yang),한동수(Dong Soo Han),손주현(Joo Hyun Sohn),김순길(Soon Kil Kim),김호중(Ho Jung Kim),고희관(Hee Kwan Koh),정자헌(Ja Hun Jung),남승우(Seung Woo Nam),백인규(In Kyu Paik),이창 대한내과학회 1997 대한내과학회지 Vol.52 No.1

N/A Objectives: The degree of hyperkalemia and effects of potassium removal by hemodialysis on the plasma potassium concentration to see the influence of nonselective beta-adrenergic blockade(carteolol) and ACE inhibitor(captopril) on patients in maintenance hemodialysis were evaluated. Methods: This study was done on 16 patients with end-stage renal disease undergoing maintenance hemodialysis. These patients were classified two groups; group 1-patients with carteolol or captopril(9 patients) and group 2-patients without medication(7 patients). Measurement of plasma potassium and arterial blood gas analyses were performed at pre-dialysis and during hemodialysis(4 hours). To analysis the distribution of potassium kinetics during hemodialysis, dialysis potassium clearance rate was introduced in this study. Results: 1) Among 16 patients studied, the mean age was 43 years old and the ratio of male to female was 2: 1 and the mean duration of hemodialysis was 17.9 months. The underlying cause of end-stage renal disease was chronic glomerulonephritis in the most patients. 2) The mean predialysis plasma potassium concentration of all patients, group 1 on medication, and group 2 without medication was 5.13±1.04mEq/L, 5.67±1.01mEq/L and 4.410.55mEq/L, with high significance(p<0.001) between groups 1 and 2. 3) The mean postdialysis plasma potassium concentration of group 1 on medication and group 2 without medication was 348±0.40mEq/L and 3.39±0.56mEq/L with insignificance between groups 1 and 2. 4) The pre- and post-dialysis concentration of plasma sodium, pH and bicarbonate between group 1 and group 2 was similar except glucose. 5) Despite the fall in absolute plasma concentration in group 1 more than twice than in group 2, the difference in dialysis potassium clearance rate measured at 1 hour of hemodialysis in group 1 compared to that of group 2 was only 12M. Conclusion: These data are consistent with at least a two-compartment distribution of plasma potassium rather than single pool in addition to frequent hyperkalemia on maintenance hemodialysis on nonselective beta-adrenergic blocker or ACE inhibitor contributed to partial impairment of extrarenal transcellular shifts of potassium during inter- and intra-dialytic phase.

간세포암종 환자에서 간동맥색전술후 혈청 Angiogenin , bFGF 및 EGF의 상승

이숭환(Soong Hwan Lee),조용현(Yong Hyeon Jo),김병훈(Byoung Hun Kim),김홍주(Hong Ju Kim),이창화(Chang Hwa Lee),이재영(Jae Young Lee),남승우(Seung Woo Nam),백인규(In Kyu Paik),박찬현(Chan Hyun Park),이동후(Dong Hoo Lee) 대한소화기학회 1996 대한소화기학회지 Vol.28 No.3

N/A Background/Aims: Angiogenesis, the induction of the formation of blood vessels, is critical to the development, progression, and metastasis of animal and human tumors. Tumor cells may produce factors which either induce or inhibit angiogenesis and that the onset of angiogenic activity is determined by the balance of these factors may be generalizable to other tumors. Hepatocellular carcinoma is hypervascular tumor and transcatheter arterial embolization(TAE) is widely used to treat patients with unresectable hepatocellular carcinoma. To evaluate changes of angiogenic factors after TAE for hepatocellular carcinoma, we investigated serum angiogenin, bFGF and EGF at the pre-TAE and the post-TAE among the seven patients with hepatocelluar carcinoma. Methods: Angiogenin, bFGF and EGF concentrations in sera were measured at pre-TAE and 3 days after TAE by employing ELISA. The statistical analysis was evaluated by Student's t-test. Results: 1) Angiogenin concentration at pre-TAE and post-TAE was 197.57+28.82ng/ml(mean+standard error) and 225.89>32.06ng/ml, respectively(p=0.022). 2) bFGF concentration at pre-TAE and post-TAE was 6.71+1.83 pg/ml and 21.68+5.58 pg/ml, respectively(p=0.036). 3) EGF concentra- tion at pre-TAE and post-TAE was 195.09+40.29 pg/rnl and 258.11+36.12 pg/ml, respectively (p=0.014). No correlation, however, was present among angiogenin, bFGF, and EGF at both of pre-TAE and post-TAE state. Conclusions: These results indicate that certain angiogenic factors, such as angiogenin, bFGF, EGF, were increased significantly at post-TAE in comparison to pre- TAE, which may reduce the therapeutic effect of TAE for hepatocellular carcinoma. We suggested that the tissue injury/destruction associated with ischemia by hepatic artery embolization are generally considered to represent a fundamental stimulus for angiogenesis in post-TAE. Therefore, suppression of angiogenesis at post-TAE may be important in improving survival for the patients with hepatocellular carcinoma since tumor growth and metastasis are angiogenesis-dependent. (Korean J Gastroenterol 1996; 28:391 - 401)

합성피혁 스킨용 폴리우레탄 수분산체의 제조 및 primerless 접착 특성 연구

김은지,백인규,박재형,Kim, Eun Ji,Paik, In Kyu,Park, Jae Hyung 한국섬유공학회 2017 한국섬유공학회지 Vol.54 No.1

Shoes, made up of an upper shoe, insole, and outsole, are manufactured by adhering each of the parts together. It has been proven that pre-treating each of these parts greatly improves the adhesive strength when adhering them together. However, harmful materials such as volatile organic compounds (VOCs) are often emitted from organic solvents during pre-treatment, causing environmental concerns such as air pollution in the workplace. Therefore, this study was conducted to develop a water-based polyurethane skin resin for fabricating a synthetic leather and textile adhesive that has greater adhesive strength and does not require pre-treatment. To achieve this, two waterborne polyurethane solutions, one with excellent thermal resistance and light fastness (waterborne polyurethane A) and the other with high adhesive strength (waterborne polyurethane B), were mixed to impart the superior characteristics of each to a third waterborne polyurethane solution. Through this approach, we were able to successfully fabricate an excellent waterborne leather polyurethane resin with ethylene vinyl acetate (EVA) for midsoles (peel strength: 2.9 kgf/cm) and for rubber out-soles (peel strength: 2.4 kgf/cm) without using pre-treatment.

피혁산업 부산물에 의한 용존 Au(3) 제거 특성

김근한 ( Keun Han Kim ),백인규 ( In Kyu Paik ),장윤영 ( Yoon Young Chang ),양재규 ( Jae Kyu Yang ) 한국수처리학회 2010 한국수처리학회지 Vol.18 No.2

Starch를 원료로 알데하이드 탄닝제의 제조 공정 연구

박재형(Jae Hyung Park),백인규(In Kyu Paik),윤종국(Jong Kuk Yun),조도광(Do Kwang Cho),정운길(Woon Gil Jung) 한국청정기술학회 2005 청정기술 Vol.11 No.1

피혁용 크롬 대체 친환경 유제 공정 처리제 제조 및 응용

박재형(Jae Hyung park),백인규(In Kyu Paik),윤종국(Jong Kuk Yun),조도광(Do Kwang Cho),조남수(Nam Soo Cho) 한국청정기술학회 2005 청정기술 Vol.11 No.2

피혁물로부터 동물성 유지와 단백질의 호l수 및 재자원화 기술 연구

윤종국(Jong-Kook Yun),백인규(In-Kyu Paik),조도광(Do-Kwang Cho),박재형(Jae-Hyung Park),최주현(Ju-Hyun Choi) 유기성자원학회 2002 유기물자원화 Vol.10 No.3

Each kinds of the leather process wastes which is occurred in the leather making process is almost more than 50% on the basis of the raw hide. The emitted process wastes are important oil and fars and prorein resources because they are composed of animal oil and fats and fìbrous protein. But most of them are incinεrate or filled up simply as the indusuial wastes without applying to recycling into the other use. Thus the ptoblems of environmental pollution are becoming more crirical and the processing cost of the leather process wastes (40,OOO~ 60,OOO won) is a heavy burden on the production cost. Because the organic wastes such as f1eshing scrap, pelt scrap are high fetid, irs unlawful abandonment without being processed properly causes the occurrence of secondary pollution byan offensive odor and leakage of waste water. Thus we made the re-resource experiments in order to resolve this problems. The principal comems of this srudy are to process the collecred leather wasre scrape through separate the oil and far ingrediems with varlous properη by processing various chemicals and enzymes on rhe next effecror. The re-resource application of separated oil and fat ingrediems produced chemical for leather applicable ro manufacturing process of leather through chemical transformation process(sulphation reaction, sulphitation reaction etc.) of oil and fats. 피혁 제조 공정에서 발생되는 각종 피혁 가공 폐기물의 발생율은 원료피 기준 거의 50% 이상이며 배출되는 피혁 가공 폐기물들은 50% 이상의 수분을 함유한 동물성 유지 및 섬유상 단백질로 구성되어 있어 귀중한 유지 및 단백질 자원임에도 불구하고 다른 용도로의 재활용 적용 없이 대부분 산업 폐기물로 소각하거나 단순 매립 처리하고 있다. 이로 인해 이 들 피혁 가공 폐기물에 의한 환경오염 문제가 날로 심각해져 가고 있는 실정 이며 피혁 가공폐기물에 대한 과다한 처리비용(4만원 ~6만원/Ton)으로 생산 원가 측면에 서도 큰 부담이 되고 있다. 더욱이 Fleshing scrap, Pelt scrap과 같은 유기성 폐기물의 경우는 부패성이 높아 적절하게 처리되지 않고 불법투기될 경우에 발생하는 악취, 오수 누출 등에 의한 2차 오염이 발생됨으로 이러한 문제점을 해결하고자 재자원화 실험을 실시하여 유지 및 단백질의 재회수 기술 개발과 이를 이용한 재 자원화 기술 개발로 피혁 처리용 약품을 제조하였다.

폴리비닐알콜/수분산 에틸렌-아크릴산 공중합체 블렌딩 필름의 내수성 및 수분/산소 차단성 연구

김은지 ( Eun Ji Kim ),박재형 ( Jae Hyung Park ),백인규 ( In Kyu Paik ) 한국공업화학회 2012 공업화학 Vol.23 No.2

에틸렌-아크릴산 공중합체(EAA)를 물에 분산시킨 후, 폴리(비닐 알코올) (PVA) 수용액과 블렌딩하여 내수성 및 차단성이 향상된 필름을 제조하였다. EAA의 함량에 따라 제조된 필름으로 열적-기계적 성질, 접촉각, 수분 투과율, 산소 투과율을 측정하였고 내수성에 대한 평가 실험도 진행하였다. 필름의 인장강도는 9.16~11.75 kg/mm2으로 PVA와 큰 차이가 없었으며, 경도의 경우는 EAA 함량에 따라 값이 점점 증가하였다. 유리전이 온도와 용융 온도는 약간 향상되었다. PVA/EAA의 비율이 90/10인 블렌딩 필름의 경우 Swelling 109%, Solubility 0%로 측정되어 PVA에 비하여 내수성이 개선되었음을 확인할 수 있었다. 또한, PVA/EAA의 비율이 90/10인 블렌딩 필름(두께 2.5 μm)을 PET 필름(두께 50 μm) 위에 코팅하여 제조된 필름의 수분 투과율과 산소 투과율은 각각 9.1 g/m2/day과 2.0 cc/m2/day으로 측정되었다. Blending films having enhanced water-resistance and barrier properties were prepared using the mixtures of poly(vinyl alcohol) (PVA) aqueous solution and poly(ethylene-co-acrylic acid) (EAA) dispersed in water. Thermal-mechanical properties, contact angles, water-vapor transmission rates (WVTR) and oxygen transmission rates (O2TR) were measured with the content of EAA of blending films, and their water-resistance was evaluated. The tensile strength of the films was found to be 9.16~11.75 kg/mm 2 which showed no significant difference compared with that of PVA, and the hardness increased with the content of EAA. The glass transition temperature and melting temperature of the blending films were slightly improved. The film prepared with PVA/EAA (= 90/10), of which the swelling and solubility were measured to be 109 and 0%, respectively, showed improved water-resistance. The WVTR and O2TR for the PET film (thickness 50 μm) coated with PVA/EAA (= 90/10) film (thickness 2.5 μm) were measured to be 9.1 g/m 2 /day and 2.0 cc/m 2 /day, respectively.