도두(刀豆)의 조혈증진 작용에 관한 연구

김승형 ( Seung Hyung Kim ),김근회 ( Kun Hoae Kim ),지경엽 ( Gyeong Yup Chi ),조인식 ( In Sik Cho ),김한영 ( Han Young Kim ),이영철 ( Young Cheol Lee ) 대한본초학회 2012 大韓本草學會誌 Vol.27 No.4

Objective : Canavalia gladiata DC semen (CGS) have been used to improve hematopoietic activity. In the current study, we investigated whether CGS regulate hemato-potentiating function using hematopoietic stem cells (HSCs) as a testing system. Methods : HSCs isolated from femur in mice with leukopenia and thrombocytopenia induced induced by CTX. Then, Real-time PCR was performed to measure the mRNA expression and hematopoietic related gene (EPO, IL-3, SCF, c-kit, GM-CSF), the phoaphorylation of GATA-1 and STAT-5a/b were observed by ELISA method, and the number of granulocyte erythrocyte monocyte macrophage colony-forming units (CFU-GEMM) and erythroid burst forming units (BFU-E), semisolid clonogenic assay was performed. Result : When HSCs were treated with CGS, the expression of hematopoietic related genes (EPO, IL-3, SCF, c-kit, and GM-CSF) were significantly increased at the levels of mRNA as well as production in HSCs. Additionally, CGS enhanced phosphorylation of STAT-1 and signal transducer and activator of transcription-5a/b (STAT-5a/b) in HSCs. Furthermore, CGS significantly enhanced the growth rate of granulocyte erythrocyte monocyte macrophage colony-forming units (CFU-GEMM) and erythroid burst forming units (BFU-E) in vitro. Conclusion : These result suggest that CGS has hematopoietic enhancement via hematopoietic cytokine -mediated GATA-1/STAT-5a/b pathway.

생쥐에서 ovalbumin과 디젤배기가스 입자로 유도된 기도염증과 기도과민성에 대한 반하 물 추출물의 항천식 효과

김승형 ( Seung Hyung Kim ),임흥빈 ( Heung Bin Lim ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.1

Objectives : Asthma is a chronic, complex respiratory disease, caused by airway obstruction, airway eosinophilic inflammation(AEI), and airway hyperresponsiveness(AHR). This study was conducted to determine whether oral administration of crude water extracts of Pinellia ternate Breitenbach(PTB) has an antiasthmatic potential in the treatment of asthma in mice, Methods : Asthmatic AEI and AHR were induced by systemic sensitization to ovalbumin(OVA) by intratracheal instillation with 0.1 mg/mL suspension of diesel exhaust particles(DEP) once a week for 10 weeks in BALB/c mice. Crude PTB water extracts(50 mg/kg and 200 mg/kg) were orally administered 5 times a week for 10 weeks. Cyclosporin(10 mg/kg) was administrered the same manner as a positive control, Results : Long-term treatment with crude PTB water extracts suppressed the infiltration of inflammatory cells, including eosinophils, into airways from blood. It also reduced asthmatic AEI and AHR by attenuating the increase in the levels of cytokines such as interleukin(IL)-4, IL-5 and IL-13 in bronchoalveolar lavage fluid(BALF), as well as the levels of histamine and OVA-specific IgE in blood. However, the effect of crude PTB water extracts(200 mg/kg) was not likely to be stronger than that of cyclosporin(10 mg/kg),Conclusion : These results suggest that crude PTB water extracts have inhibitory effects on AEI and AHR in a mouse model of asthma and may act as a potential Th2 cytokine antagonist, and have a therapeutic effect on allergic asthma.

홍의 추출물의 항염작용 및 그 기전 연구

김종민 ( Kim Jong Min ),김승형 ( Kim Seung Hyung ),양원경 ( Yang Won Kyung ),정택근 ( Jung Taek Geun ),김세란 ( Kim Se Ran ),황성준 ( Hwang Sung Joon ),유화승 ( Yoo Hwa Seung ) 대전대학교 한의학연구소 2016 한의학연구소 논문집 Vol.25 No.1

Objective : Hongyi (Formica yessensis) is the dried insect of fomicidae. In previous studies, it appeared possibilities on anti-thrombosis, preventing atherosclerosis, treating rheumatoid disease, and inhibiting hela cell. In this study, we investigated anti-inflammatory effects and mechanism of Hongyi. Methods : Hongyi A was extracted by water and made dried powder. Hongyi B was extracted by ethanol and made dried powder. We measured Nitric Oxide (NO) production on the mouse macrophages (RAW 264.7), mouse vascular endothelial cell (MOVAS) and human vascular endothelial cell (HUVEC) for anti-inflammatory effect. In addition, we conducted reverse transcription reaction (RT-PCR) for investigating the mechanism. Results : In RAW 264.7 macrophages stimulated by LPS, Hongyi A (100 ㎍/㎖) decreased NO production compared with LPS 2 ㎍/ml control group with statistical significance (p<0.05). Hongyi A (50, 100 ㎍/㎖) also decreased NO production compared with LPS 4 ㎍/ml control group with statistical significance (p<0.01). Hongyi B (50, 100 ㎍/㎖) decreased NO production compared with LPS 2 ㎍/ml control group with statistical significance (p<0.01). Hongyi B (10, 50, 100 ㎍/㎖) also decreased NO production compared with LPS 4 ㎍/ml control group with statistical significance (p<0.01, p<0.001, p<0.001). In the MOVAS, Hongyi A and B increased NO production compared with control group. In the HUVEC, Hongyi B increased NO production compared with control group. The expression of NF-κB in 12-hours MOVAS culture was decreased by Hongyi A and B (10, 50 ㎍/ml) compared with control group, but expression of IκB was increased. In the 24-hours MOVAS culture, expression of IκB was significantly increased. The expression of NF-κB in 12 hours HUVEC culture was decreased by Hongyi A and B compared with control group, but expression of IκB was increased. Hongyi B also increased eNOS mRNA gene expression. Conclusions : Hongyi A and B showed anti-inflammatory effect in mouse macrophages with the activation of vascular endothelial cell through NO production in MOVAS and HUVEC repectively. Honyi B showed superior effect than Hongyi A, but additonal mechanism study should be conducted.

Monosodium iodoacetate로 유도된 골관절염 동물모델에서 상기생 물추출물의 효과

김중선 ( Joong Sun Kim ),이아영 ( A Yeong Lee ),문병철 ( Byeong Cheol Moon ),김효선 ( Hyo Seon Kim ),김욱진 ( Wook Jin Kim ),김철 ( Chul Kim ),최고야 ( Goya Choi ),김승형 ( Seung-hyung Kim ),천진미 ( Jin Mi Chun ) 대한본초학회 2018 大韓本草學會誌 Vol.33 No.5

Objectives : The aim of this study was to investigate the protective effects of an aqueous extract from Taxillus chinensis (DC.) Danser (TCE) in Monosodium iodoacetate (MIA)-induced osteoarthritis (OA) rat model. Methods : Sprague Dawley male rats were divided into the following four groups (n=6 per group): Normal (saline control), MIA (MIA-induced OA with vehicle), TCE (MIA-induced with TCE treatment), and IM (MIA-induced with indomethacin treatment). Rats in which OA was induced by MIA were treated with TCE (200 ㎎/㎏) or indomethacin (1 ㎎/㎏) for 4 weeks. Weight-bearing on the hind legs and body weights were measured weekly. At the end of the experiment (3 weeks after MIA injection), serum aspartate aminotransferase and alanine aminotransferase levels were measured to assess the liver toxicity induced by TCE. Its effects on serum inflammatory cytokine levels and tissue histopathology were also evaluated. Results : TCE restored the hind limb weight-bearing distribution. Serum levels of Interleukin 6 (IL-6), Tumor necrosis factor alpha (TNF-α) and Leukotriene B4 (LTB4) were significantly higher in the MIA group than in the Normal group, but serum IL-6 levels were significantly lower in the TCE group. In the TCE group, the synovial membrane was protected in hematoxylin and eosin and Safranin-O staining, respectively. Conclusions : TCE recovered the hind paw weight bearing distribution, inhibited the production of inflammatory cytokine, and protected synovial tissue and cartilage in the OA rat model. Therefore, TCE appears to be an effective therapeutic agent for treating OA and OA-related symptoms.

Sca-1+골수조혈세포에서 JAK2/STAT5/GATA-1 신호전달 경로를 통한 다채, 도두 그리고 두 조합물에 의한 조혈증진 조절에 관한 연구

김근회 ( Kun Hoae Kim ),김승형 ( Seung Hyung Kim ),조인식 ( In Sik Cho ),김한영 ( Han Young Kim ),김동선 ( Dong Seon Kim ),이영철 ( Young Cheol Lee ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.4

Objectives : Brassica campestris var narinosa (BCN), Canavalia gladiata DC semen (CGD) and their combinational prescription (BCN+CGD) have been use to demonstrate to regulate hematopoiesis. In the current study, we investigated whether Brassica campestris var narinosa, Canavalia gladiata DC semen and their combinational prescription is related to hemato-potentiating function using Sca-1+ hematopoietic stem cells (Sca-1+HSCs) as a testing system. Methods : Sca-1+HSCs isolated from femur in C57bl/6 mice with leukopenia and thrombocytopenia induced by cyclophosphamide (CTX). Then, Real-time PCR was performed to measure the mRNA expression, ELISA and haematopoiesis-related gene (EPO, TPO, IL-3, SCF, c-kit, GM-CSF), the phosphorylation of JAK2, GATA-1 and STAT-5a/b were observed by western blot, and the numbers of CD117+/Sca-1+ cell and the number of granulocyte erythrocyte monocyte macrophage colony-forming units (CFU-GEMM) and erythroid burst forming units (BFU-E), semisolid clonogenic assay was performed. Result : When Sca-1+HSCs were treated with Brassica campestris var narinosa, Canavalia gladiata DC semen and their combinational prescription with rIL-3/rSCF, the expression of haematopoiesis-related (EPO, TPO, IL-3, SCF, c-kit, and GM-CSF) were significantly increased at the levels of mRNA as well as production in Sca-1+HSCs. Additionally, CGS enhanced phosphorylation of JAK2, GATA-1, and signal transducer and activator of transcription-5a/b (STAT-5a/b) in Sca-1+HSCs. Furthermore, their combinational prescription (BCN+CGD) significantly enhanced the growth rate of granulocyte erythrocyte monocyte macrophage colony-forming units (CFU-GEMM) and erythroid burst forming units (BFU-E) in vitro. Conclusion : These result suggest that Brassica campestris var narinosa (BCN) and Canavalia gladiata DC have hematopoietic enhancement via hematopoietic cytokine-mediated JAK2/GATA-1/ STAT-5a/b pathway, and their combinational prescription (BCN+CGD) has superior hematopoietic enhancement to those of individual extracts.

종양이식 생쥐모델에서 도두(刀豆), 우방근(牛蒡根) 추출물의 대장암 억제 효과

장지혜,지건영,최형석,양원경,김한영,김근회,강형식,이영철,김승형,Jang, Ji-Hye,Ji, Kon-Young,Choi, Hyung-Seok,Yang, Won-Kyung,Kim, Han-Young,Kim, Kun-hoae,Kang, Hyung-Sik,Lee, Young-Cheol,Kim, Seung-Hyung 대한본초학회 2017 大韓本草學會誌 Vol.32 No.5

Objective : In the present study, we examined whether Canavalia gladiata D.C. (CG) and Arctium lappa L., Redix (AL) mixture (CGAL), their components, lupeol and chicoric acid, regulate immune system and suppress the tumor in vitro and in vivo. Methods : LPS-induced reactive oxygen species (ROS) and nitric oxide (NO) were measured after treatment with CG extract (CGE), CGAL, lupeol, chicoric acid and lupeol and chicoric acid mixture (lupeol+CA) in Raw264.7 cell. To determine the effect of CGE on immune responses, immune cell population and IgG production were assessed in mice. To investigate the effect of CGAL and their component on anti-tumor activity, tumor volume and weight were measured, cell cycles and immune cell population were analyzed in MC38 injected tumor bearing mice. Also, NK cell activity was determined in splenocyte isolated from tumor bearing mice. Results : CGE, CGAL, lupeol, chicoric acid and lupeol+CA decreased the LPS-induced ROS and NO production without cell toxicity in RAW264.7 cells. CGE increased the immune cell populations of $CD4^+T$, $CD8^+T$ and macrophages in various immune organ of mice. In tumor bearing mice, CGAL, lupeol, chicoric acid and lupeol+CA suppressed tumor volume and weight. In cell cycle analysis, they decreased the percentages of S phase. In addition, CGAL, lupeol, chicoric acid and lupeol+CA immune cell populations of $CD4^+T$, $CD8^+Tcell$, NK cell and macrophage in tumor as well as NK cell activity. Conclusion : CGAL and its compounds may enhance immune responses and suppress tumor growth, and may be capable of developing health functional foods.

경피적 도관 배액술과 경화요법으로 치료한 후복막 낭성 림프관종 1예

강현식,김승형,김봉수,강기수,Kang, Hyun-Sik,Kim, Seung-Hyung,Kim, Bong-Soo,Kang, Ki-Soo 대한소아소화기영양학회 2010 Pediatric gastroenterology, hepatology & nutrition Vol.13 No.1

후복막 낭성 림프관종은 양성종양으로 매우 드문 질환이다. 수술적 절제가 원칙이나, 경화요법을 우선적으로 고려할 수도 있다. 9세 남자가 병원 방문 4일 전부터점점 심해지는 복통을 주소로 입원하였다. 혈색소 농도가 입원 당일 12.8 g/dL에서, 입원 3병일째 10.6 g/dL로 감소한 소견이 있었다. 복부 전산화 단층촬영에서 크고 종양내부에 격막이 있는 분엽상의 후복막 낭종($10{\times}9.5{\times}5cm$) 한 개가 좌측 신장 주위 공간에서 관찰되었다. 종양 내부에는 출혈이 동반되어 있었으며, 하장간막정맥이 종양에 의해 둘러싸여져 있었다. 수술적 치료의 위험도가 매우 높을 것으로 판단되어 낭종조영술을 시행하였고, 경피적 도관 배액술과 에탄올 경화요법을 시행하였다. 추적 검사로 시행한 복부 전산화 단층촬영에서 종양의 크기가 현저히 감소된 소견을 보였다. 환자는 퇴원 후 13개월 째 현재까지 낭종의 재발없이 건강하게 지내고 있다. 결론적으로, 수술적 치료의 위험이 높은 후복막 낭성 림프관종이 있는 경우 경피적 도관 배액술과 경화요법을 우선적으로 고려해야 할 것이다. Retroperitoneal cystic lymphangiomas are benign, extremely rare tumors. Although surgical resection is the treatment of choice, sclerotherapy should be considered initially. A 9-year-old boy was admitted due do worsening abdominal pain of 4 days duration. Serial complete blood counts revealed a hemoglobin level of 12.8 g/dL on admission to 10.6 g/dL on hospital day 3. An abdominal computed tomography (CT) scan showed a large, lobulated, septated, retroperitoneal cystic mass ($10{\times}9.5{\times}5cm$) in the left anterior pararenal space with intracystic hemorrhage surrounding the inferior mesenteric vein (IMV). Because of the high operative risk, we performed a tubogram of the cystic mass, percutaneous catheter drainage (PCD), and ethanol sclerotherapy. The follow-up abdominal CT scan showed that the cystic mass had decreased in size. He is well without relapse of the retroperitoneal cystic mass for 13 months after discharge. Sclerotherapy with PCD should be considered as initial therapy for patients with retroperitoneal cystic lymphangiomas at high surgical risk.

소염약침액이 Collagen II 유발 관절염 mouse의 TNF-α, IFN-γ 생성 및 비장세포 증식에 미치는 영향

유화승,윤대환,김승형,임종순,Yoo, Hwa-Seung,Youn, Dae-Hwan,Kim, Seung-Hyung,Lim, Jong-Soon 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.2

Objective : The aim is to examine the effect of Soyeum Pharmacopuncture (SPP) on collagen-induced arthritis (CIA) in DBA/1OlaHsd mice. Methods : To determine the effect of SPP on chronic IFNlammatory joint disease, we induced CIA in DBA/1OlaHsd mice by immunization with bovine type II collagen. Animals were treated with intraperitoneal injection doses of 2 mg/kg of SPP, beginning 3 days before the expected onset of disease symptoms. Inhibitory Effects of SPP were observed by serum levels of TNF-${\alpha}$, and IFN-${\gamma}$,,, or cell proliferation in the spleen cell culture and histological examination of knee joint. Results : In the CIA Mice, serum levels of TNF-${\alpha}$, and IFN-${\gamma}$,, production in the spleen cell culture were reduced. At the histopathological examination of knee joint, chondropathy of cartilage in the synovial joint in the SP group was repaired while compared with control group. Conclusion : These results suggest that the SPP may be effective for the prevention and treatment of rheumatoid arthritis disease.

Antiplatelet Agents from the Extracts of Phyllostachys pubescensleaves and Prunus mume Fruits

Dong Seon Kim(김동선),Eunjung Son(손은정),Won Kyung Yang(양원경),Seung Hyung Kim(김승형),Jimin Cha(차지민) 한국약용작물학회 2017 한국약용작물학회 학술대회논문집 Vol.2017 No.2

고요산동물에서의 익지인의 요산저하 효과

이영실,김지연,김승형,김동선,Lee, Young-Sil,Kim, Jiyoun,Kim, Seung-Hyung,Kim, Dong-Seon 대한본초학회 2017 大韓本草學會誌 Vol.32 No.6

Objective : Hyperuricemia is a metabolic disease characterized by elevated blood uric acid levels, and its prevalence is rapidly increasing worldwide. Alpiniae Oxyphyllae Fructus (AO) belonging to Zingiberaceae is one of well-known traditional medicines in China and Korea, and has been used to treat intestinal disorders, urosis, diuresis, and chronic glomerulonephritis traditionally. However, the effect of AO has not been studied. In this study we investigated the anti-hyperuricemic effect of AO, and the mechanisms underlying the effect in potassium oxonate (PO)-induced hyperuricemic rats. Methods : To examine the anti-hyperuricemic effects of the AO extract, serum uric acid levels were analyzed in normal and PO-induced hyperuricemic rats. The mechanism underlying the effects of the AO extract on uric acid levels was studied through xanthine oxidase (XOD) activity test and uric acid uptake assay in vitro. The chemical finger printing of the AO extract was analyzed using HPLC-DAD. Results : The AO extract significantly reduced serum uric acid levels in normal as well as PO-induced hyperuricemic rats. It also significantly inhibited the uptake of uric acid in oocytyes and human embryonic kidney cells (HEK293) expressing urate transporter (URAT)1, but not XOD activity in vitro. The chemical finger printing analysis of the AO extract showed nootkatone as a main component. Conclusion : The AO extract exhibits anti-hyperuricemic effects, and these effect were accompanied by increasing excretion of uric acid in kidney. Therefore, the AO extract could be used for prevention or treatment of hyperuicemia and gout.