DNA 분석법에 의한 한우고기 판별

오홍록,이창수,상병찬,송광택 충남대학교 농업과학연구소 2006 농업과학연구 Vol.33 No.1

This study was deal with the development of breed-specific DNA marker which is able to identify Hanwoo and European cattle breeds(Non-Hanwoo) meat. Genetic differentiation between Korean cattle(Hanwoo) and European cattle breeds was examined by Random Amplified Polymorphic DNA(RAPD) analysis. The RAPD patterns were identical among Non-Hanwoo, such as Holstein, Hereford. Aberdeen Angus. Brown Swiss. Limousin or Simmental. but the above pattern was different from that of Hanwoo. All bands detected in the Hanwoo samples were observed in Non-Hanwoo cattle samples, but one of the common bands found in samples was not detected in the Hanwoo samples. The band(1.4kb) may be useful as a marker for identifying a meat of Hanwoo from imported cattle meat. Actually, the detection of the DNA marker was tested by DNA analysis with 929 samples which were prepared from bloods of 673 Hanwoo cattles and 141 Holstein cattles, from 115 imported cattle meats. The DNA marker was absent in 644 of 673 Hanwoo cattles (96%) but present in 245 of 256 Non-Hanwoo cattles (95%). These results show that the DNA marker is effective to characterize Hanwoo and Non-Hanwoo meat by its detection. This DNA marker, however, was not useful in detecting unwanted crossbreeding between two cattle breeds, because the band pattern in hybrid cattle shows one of two band patterns in Hanwoo and Non-Hanwoo.

硏究開發課題의 選定과 評價에 관한 硏究

李五範,李光澤,高德弼,金炳泰 청주대학교 경상대학 1985 經商論叢 Vol.26 No.-

우리 나라 농장 사육 타조 알의 난질 조사

송광택,오홍록 충남대학교 농업과학연구소 2002 농업과학연구 Vol.29 No.1

The fresh eggs of African ostrich in Korea were used in this study to investigate their interior and exterior quality characteristics. In ostrich egg shape characteristics, long and short length were measured as 15.1∼16.1 ㎝ and 12.1∼13.6 ㎝, respectively. The egg shape index was calculated as 81.0 and the egg shell showed a more gloss white in color than that of hen egg. Calculated breaking strength of egg shell and egg shell surface based on the equation were 41.155 ㎏/㎤ and 804.7 ㎠, respectively. Average egg shell thickness including egg shell membrane was 1.89 ㎜ and there are no differences among parts of the shell. The egg shell membrane thickness was 0.10 ㎜ showing relatively thick in equator compared with that of blunt end. The average number of egg shell pores was counted as 19.7/㎠ showing relatively high number of egg shell pores at point end and the total number of egg shell pores in an egg was estimated as 15,241. The relative portion of each parts based on egg weight was estimated as 25.1% for egg york, 58.2% for egg white, 16.7% for egg shell and the ratio of egg yolk to egg white(Y/W) was 0.43. The average diameter and the average height of ostrich egg white were 19.7 ㎝ and 13.2 ㎜, respectively. Calculated the H.U (Haugh Unit) was -69.7 and the pH was 8.3. In ostrich egg yolk, the diameter, the height, the egg yolk index, and it's pH were calculated as 14.4 ㎝, 29.4 ㎜, 0.20, and 6.6, respectively. And the egg yolk color showed a more thin in yellow chroma compared with that of hen egg as due to it's thicker membrane.

精神分裂症患者의 Schneider 一級症狀과 思考障碍

元鎬澤,朴東洙,吳相宇,金光日 漢陽大學校 精神健康硏究 1983 精神健康硏究 Vol.1 No.-

발포폴리스티렌비드를 사용한 경량콘크리트의 역학적 특성에 관한 실험적 연구

주재금,이종찬,오세출,유택동,정광량,서치호 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

The purpose of this study is to obtain basic data on the properties of the lightweight concrete within expanded polystyrene beads for various applications in the field. Inquiring into the change of the properties as altering cement matrix mixture and the capacity mixture ratio of expanded polystyrene beads, the mixture plane of this study is set up as W/C is fixed 50%, expanded polystyrene beads is increased 10% from 0% to 80% per 1m³ and the ratio of cement:sand in the cement matrix is 1:0, 1:0.5, 1:1. The results of this study are as follow. The slump as changing the mixture ratio of expanded polystyrene beads decrease smoothly until mixture ratio 50% but decrease rapidly being in excess of 50%. The density decrease regularly as the mixture ratio of expanded polystyrene beads increase but the ratio of strength decrease. The difference of cement matrix. The strength of cement matrix having more sand mixture ratio relatively decrease rapidly as increasing the mixture ratio of expanded polystyrene beads. It is judged decreased strength phenomenon as the quantity of coherent material created in the mixture decrease rapidly.

과산화수소의 농도와 적용시간이 상아질의 깊이에 따라 레진 수복물의 미세인장결합강도에 미치는 영향

손정룡,이계영,강유미,오영택,이광원,김태균 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.5

본 연구의 목적은 심부 상아질과 치수실 상아질에 H₂O₂를 각기 다른 적용시간과 농도로 적용하였을 때, 레진수복물과의 미세인장결합강도에 미치는 영향을 평가하는 것이다. 준비된 각각의 치아에 전형적인 근관와동을 형성하고 다음과 같이 무작위로 1개의 대조군과, 4개의 실험군으로 나누었다: 1군, 전처리를 시행하지 않았다; 2군, 20% H₂O₂를 20분, 10분, 5분간 적용하였다; 3군, 10% 를 20분, 10분, 5분간 적용하였다; 4군 5% H₂O₂를 20분, 10분, 5분간 적용하였다; 5군, 2.5% H₂O₂를 20분, 10분, 5분간 적용하였다. 위와 같이 처리한 후 와동 내를 Superbond C&B(Sunmedical, Co., Shiga, Japan)로 충전하였다. 각 치아는 증류수에 24시간 저장한 뒤 심부 상아질과 치수실 상아질로 잘라 미세인장결합강도 시험을 시행하였다. 각 군에서 측정된 미세인장결합강도를 3-way ANOVA와 Tukey post hoc test로 통계 처리하였다(p < 0.05). 실험 결과 모든 군에서 심부 상아질(D1)의 미세인장 결합강도가 치수실 상아질(D2)보다 높게 나타났다. 평균 결합강도는 H₂O₂의 농도와 적용 시간이 증가할수록 감소하였다. 통계분석에서 상아질의 깊이, H₂O₂농도와 적용 시간이라는 세 가지 변수간의 교호작용 효과는 없으나(p > 0.05). 상아질의 깊이와 H₂O₂농도 사이, H₂O₂농도와 적용 시간 사이에서는 교호작용 효과가 있었다(p < 0.05). SEM 관찰에서는 H₂O₂의 농도가 증가할수록 상아세관이 좀 더 개방된 양상을 보였다. The purpose of this study was to examine the effect of hydrogen peroxide at different application time and concentrations on the microtensile bond strength of resin restorations to the deep and the pulp chamber dentin. A conventional endodontic access cavity was prepared in each tooth, and then the teeth were randomly divided into 1 control group and 4 experimental groups as follows: Group 1, non treated; Group 2, with 20% Hydrogen peroxide (H₂O₂); Group 3, with 10% H₂O₂; Group 4, with 5% H₂O₂; Group 5, with 2.5% H₂O₂; the teeth of all groups except group 1 were treated for 20, 10, and 5min. The treated teeth were filled using a Superbond C&B (Sun medical Co., Shiga, Japan). Thereafter, the specimens were stored in distilled water at for 24-hours and then sectioned into the deep and the chamber dentin. The microtensile bond strength values of each group were analyzed by 3-way ANOVA and Tukey post hoc test(p < 0.05). In this study, the microtensile bond strength of the deep dentin (D1) was significantly greater than that of the pulp chamber dentin (D2) in the all groups tested. The average of microtensile bond strength was decreased as the concentration and the application time of H₂O₂ were increased. Analysis showed significant correlation effect not only between the depth of the dentin and the concentration of H₂O₂ but also between the concentration of H₂O₂ and the application time(p < 0.05), while no significant difference existed among these three variables(p > 0.05). The higher H₂O₂ concentration, the more opened dentinal tubules under a scanning electron microscope(SEM) examination.

폐 격리증의 임상적 특성

오광택(Kwang Taek Oh),김연수(Yeon Soo Kim),김병철(Byung Cheol Kim),문두섭(Doo Seop Moon),이동석(Dong Suck Lee),이경상(Kyung Sang Lee),양석철(Suck Chul Yang),윤호주(Ho Joo Yoon),신동호(Dong Ho Shin),박성수(Sung Soo Park),이정희(Jung H 대한내과학회 1996 대한내과학회지 Vol.51 No.5

N/A Objectives: Pulmonary sequestration is a relatively rare congenital anomaly. It is a cystic mass of nonfunctioning lung tissue which lacks an obvious communication with the tracheobronchial tree and which receives all or most of its arterial blood supply from anomalous systemic vessels. It has a wide spectrum of various clinical manifestations. But high index of suspicion of sequestration with the aid of characteristic recurrent and longstanding symptoms, its location and invasive or non-invasive diagnostic tools is the key to early diagnosis and successful treatment. So we performed this clinical study to evaluate clinical symptoms and diagnostic methods of pulmonary sequestration. Methods: We reviewed eight cases of pulmonary sequestration seen during the period from 1982 to 1995 retrospectively to define the anatomical, clinical and radiologic features. Results: 1) The eight cases ranged in age from 11 to 35 years and show an even distribution within age decades. Sex ratio of male to female was a 1:1.67. 2) The presenting complaints were symptoms of local recurrent pulmonary infection. And also revealed unique chest pain or no any symptoms. 3) Plain X-ray findings were solitary nodules in three cases, pneumonic infiltration in two cases and cystic mass with air-fluid level, multiloculated cystic mass and solitary large cystic mass in each of the remaining three cases. 4) Preoperative diagnoses were possible in 5 cases and the other diagnoses were lung cancer in two cases and lung abscess in one case. 5) The seven cases were intralobar type and one case was extralobar type. Their location was in the lower lobe of the lung in seven cases and right upper lobe in one case. Among eight cases, the sequestrated lobe was located bilaterally in one case and the others were located unilaterally with a 2.5:1 Rt/Lt ratio. 6) Associated anomalies were esophageal cyst and bronchogenic cyst in two cases. 7) All cases were treated with lobectomy and extirpation without complication. Conclusion: We reviewed eight cases of pulmonary sequestration and its literature. These data reveal that pulmonary sequestration shows variable but characteristic clinical and radiological features. Therefore, a high index of suspicion about its characteristic clinical and radiologic features can provide us a change of early diagnosis and proper treatment of pulmonary sequestration.

소아기 류마티스 관절염의 임상적 특성에 관한 연구

오광택 ( Kwang Taek Oh ),정성수 ( Sung Soo Jung ),유태석 ( Tae Seok Yoo ),이제경 ( Je Kyung Lee ),송용호 ( Yong Ho Song ),정자헌 ( Ja Hun Jung ),고희관 ( Hee Kwan Koh ),김태환 ( Tae Hwan Kim ),전재범 ( Jae Bum Jun ),이인홍 ( In Ho 대한류마티스학회 1997 대한류마티스학회지 Vol.4 No.2

Objective: To investigate the clinical characteristics of Korean JRA and the possible profiles related to prognosis and to compare these data to pre-existing occidental reports. Methods: 140 cases of JRA patients were randomly selected from 1986 through 1995. Juvenile ankylosing spondylitis was excluded with the aid of modified New York criteria for ankylosing spondylitis. We used the classification of progression of rheumatoid arthritis (radiological stage III+IV/I+II+III+IV) as a index of prognosis. The study factors were sex, age, type, affected joints, degree of destruction of joint, anti-nuclear antibody (ANA), rheumatoid factor (RF), HLA-B27 and extra-articular manifestations. Chi-square test, One-way ANOVA test, and Pearson`s correlation coefficient were used as statistical methods. Results: Our study show results as follows: (1) Systemic onset type(11%) polyarthritis subset (66%), oligoarthritis subset (34%), oligoarthritis onset type(62%) sero(-)-HLA-B27(+) subset(66%), RF(+) subset(20%), not otherwise classified subset (14%) ANA (+)-chronic uveitis subset (0%), polyarthritis onset type(27%) RF(+) subset (66%), not otherwise classified subset(34%). (2) Sex ratio was M:F=1.8:1. (3) Age of onset was 11±3.6 years. (4) Affected joints were knee, ankle, hand in decreasing order of frequency. (5) Patients show seropositivity of RF in 31%, HLA-B27 in 54%, ANA in 8%. (6) Chronic uveitis was observed in 7 cases (5%) (all oligoarthritis onset type, M:F=6:1, age of onset 11±4.3 years, ANA(0%), HLA-B27(86%), 2 cases leading to blindness. (7) Destructive bone change was significantly more prominent in female(p<0.01), RF(+) (p<0.01), HLA-B27(-) (p<0.01), polyarthritis onset type (p=0.02 ). Conclusions: In this study, we obtained interesting results that are somewhat different from Occidental data in sex ratio(male dominance), age of onset(older age), profiles of HLA-B27(high positivity in spite of low HLA-B27 positivity in Korean (about 2.3%), low rate of destructive change), ANA (low positivity, older age of onset), chronic uveitis(low incidence, male dominance, older age of onset, high association with HLA-B27, not associated with ANA). This study suggests possible racial difference in clinical features of JRA. But for prove of racial difference, further multi-center trial and large scale epidemiological study should be done.

날개 길이방향 유동이 날개짓 운동의 공기역학적 특성에 미치는 효과

오현택(Hyun-Taek Oh),최항철(Hang-Cheol Choi),김광호(Kwang-Ho Kim),정진택(Jin Taek Chung) 한국유체기계학회 2006 유체기계 연구개발 발표회 논문집 Vol.- No.-

Flight performance of birds and insects is superior to conventional fixed wing aircrafts at low Reynolds number regime. While they flap their wings, aerodynamic characteristics such as span-wise flow and rotational circulation are appeared on the wing. To study on the effect of span-wise flow, 3-D flapping motion was compared with 2-D flapping motion. In 3-D flapping motion, the measured lift forces were increased until the angle of attack 50° at mid-stroke. But in 2-D flapping motion, the lift forces were decreased above the angle of attack 30° at mid-stroke. Also, the mean lift to drag ratio of 3-D flapping motion is higher than that of 2-D flapping motion.

날개의 종횡비가 날개 짓 운동의 공기역학적 특성에 미치는 영향

오현택(Hyun-Taek Oh),최항철(Hang-Cheol Choi),김광호(Kwang-Ho Kim),정진택(Jin Taek Chung) 한국유체기계학회 2006 유체기계 연구개발 발표회 논문집 Vol.- No.-

The lift and drag forces produced by a wing of a given cross-sectional profile are dependent on the wing planform and the angle of attack. Aspect ratio is the ratio of the wing span to the average chord. For conventional fixed wing aircrafts, high aspect ratio wings produce a higher lift to drag ratio than low ones for flight at subsonic speeds. Therefore, high aspect ratio wings are used on aircraft intended for long endurance. However, birds and insects flap their wings to fly in the air and they can change their wing motions. Their wing motions are made up of translation and rotation. Therefore, we tested flapping motions with parameters which affect rotational motion such as the angle of attack and the wing beat frequency. The half elliptic shaped wings were designed with the variation of aspect ratio from 4 to 11. The flapping device was operated in the water to reduce the wing beat frequency according to Reynolds similarity. In this study, the aerodynamic forces, the time-averaged force coefficients and the lift to drag ratio were measured at Reynolds number 15,000 to explore the aerodynamic characteristics with the variation of aspect ratio. The maximum lift coefficient was turned up at AR=8. The mean drag coefficients were almost same values at angle of attack from 10° to 40° regardless of aspect ratio, and the mean drag coefficients above angle of attack 50° were decreased according to the increase of aspect ratio. For flapping motion the maximum mean lift to drag ratio appeared at AR=8.