흰쥐에서 심정지후 뇌의 허혈-재관류 손상과 Tumor Necrosis Factor-α의 상관관계

최승필,박규남,박승현,박상현,정시경,김세경 대한응급의학회 1999 대한응급의학회지 Vol.10 No.4

Background : Tumor necrosis factor-α(TNF-α) has been thought to play a major role in neurological injury during global brain ischemia and subsequent reperfusion following resuscitation in cardiac arrest. So, we hypothesized that the elevation in TNF-α was dependent upon the duration of the global brain ischemia, and related to delayed neuronal damage. Methods : Fourteen rats were divided two groups ; 1 minute-cardiac arrest group(n=7) and 3 minute-cardiac arrest group(n=7). we induced cardiac arrest by chest compression and clamping of tracheal tube for 1 minute and 3 minutes respectively. And then, resuscitation was initiated. To measure the plasma activity of TNF-α, blood samples were drawn before and at the end of cardiac arrest, and 30, 60, 90, and 120 minutes after initiation reperfusion. At 72 hours after resuscitation, the ND(neurologic deficit) score was determined and the histopathologic outcome of hippocampal CA1 neuron was observed by the percent dead hippocampal CA1 neurons. Results : 1. TNF-α level during the early reperfusion period(<2h) was significantly increased in 3 min-cardiac arrest group compared with 1 min-cardiac arrest group(p=0.0001). 2. There was a no significant difference of neurologic deficit score between 1 min-and 3 min-cardiac arrest. 3. Percent dead hippocampal neurons were significantly increased in 3 min-cardiac arrest group compared with 1 min-cardiac arrest group(9.1±1.2% vs 1.2±0.9%, p<0.05). Conclusions : The results suggest that longer duration of global brain ischemia causes a more profound increase in plasma TNF-α level during the early reperfusion period(<2h) and more delayed neuronal damage than lessor duration of global brain ischemia, and that increase in TNF-α level during the early reperfusion period(<2h) is related to delayed neuronal damage.

외팔보 진동의 시간 최적제어

박영필,정승호 연세대학교 산업기술연구소 1986 논문집 Vol.18 No.1

The feedback controller for the active control of a cantilever beam vibration is developed using time optimal bang-bang control theory. The effects of I)the constraint of the control force magnitude and II)the actuator positions are investigated numerically. The results show that I)the magnitude of the constraint force and control time are inversely proportional, II)the control effect is increased as the actuator position moves toward the free end of the beam, and III)the controller gives Coulomb damping effect to the system.

BSCCO 시스템에서 초전도상의 특성

박용필,양승호,천민우 동신대학교 2005 論文集 Vol.15 No.-

BSCCO(Bi_(2)Sr_(2)Ca_(n-1)Cu_(n)O_(x)) 초전도 상을 이온 빔 스퍼터와 단일 타겟 DC-magnetron 스퍼터를 사용하여 제작하였다. 상형성은 n=2-3, 3-4 그리고 4-5 사이에서 이루어졌다. 스퍼터링 시 분위기 가스로 산소를 주입 하였고 제작된 박막의 상의 몰 비를 XRD를 이용하여 분석하였다. 각 상의 몰 비를 이용하여 초전도박막의 특성을 변화시킬 수 있음을 알았다. 또한, 각 상을 AFM과 SEM을 사용하여 박막의 표면을 관찰하여 표면의 불순물 상태를 알 수 있었다. BSCCO(Bi_(2)Sr_(2)Ca_(n-1)Cu_(n)O_(x)) superconducting phase were fabricated by an ion beam sputtering and single target dc-magnetron sputtering. Phase intergrowth among n=2-3, 3-4 and 4-5 phases was observed. The molar fraction of each phase in the mixed crystal of the deposited films was determined by X-ray diffraction analyses and investigated as a function of O_(2) gas pressure during sputtering. We investigated the changes of the superconducting properties by molar fraction of each phase. Also, the thin film surface observation was carried out by AFM. According to the result observing the surface of the thin film with SEM, even in case that the formed phase and the composition of the thin film agree, it can be known that there are a number of the precipitates on the surface.

완전뇌허혈후 재관류시에 국소뇌혈류, 체성감각유발전위 및 신경학적 회복에 대한 Mannitol과 Thiopental의 효과

박춘근,이상원,박영섭,최승진,허필우,정동섭,강준기,최창락 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.2

The cardiopulmonary bypass or cerebral circulation arrest is often used in the treatment of complex aneurysm or of arteriovenous malformation to decrease the risk of intraoperative aneurysm rupture. Although experimental studies have suggested that some drugs may protect the brain from ischemic injury, there are limitations in maintaining cerebral perfusion arrest without incurring neurologic deficits due to the initiation of detrimental processes including excitotoxic neuronal injury, activation of phospholipases, influx of calcium, and generation of damaging free radicals. The purpose of this study is to determine wheter mannitol or thiopental has any favorable effects on the recovery of neurologic deficits and on the regional cerebral blood flow(rCBF), somatosensory evoked potential(SEP) and electroencephalogram(EEG) in cats which underwent 15 minutes-complete global ishcemia-reperfusion. The complete global ischemia was produced in 38 cats by temporary intrathoracic occlusion of the innominate artery and the subclavian artery following ligation of bilateral mammary arteries and simultaneous induction of hypotension. The cats were allocated randomly to one of 4 treatment groups : (1) control group, 8 cats received equal volume of saline solution : (2) thiopental group, 10 cats received 45㎎/㎏ thiopental intravenously, (3) mannitol group, 10 cats received 2g/㎏ mannitol intravenously, (4) combined mannitol and thiopental group, 10 cats received equal dose of mannitol and thiopental intravenously. The drugs were administrated in a equally divided dosage before and after the ischemic episode. The results were as follos : 1) Eight animals which received saline showed the severe postischemic hypoperfusion and poor recovery of SEP and EEG, and 6 of them died within 6 hours after the ischemia. 2) Ten thiopental-treated animals also showed the severe postischemic hypoperfusion and poor recovery of SEP and EEG, and 6 of them died within 6 hours the ischemia. 3) Both mannitol-and combined treated groups showed early recovery of EEG, good recovery of SEP and EEG without the severe postischemic hypoperfusion, and 7 of 10 mannitol-treated animals and 8 of 10 combined treated animals were significantly recovered in all parameters. There were not significant differences in all parameters between the mannitol-and combined treated groups. 4) Thirty-four of 38 animals involved in this 15 minutes-ischemia resulted in the severe neurologic deficits inspite of treatment with mannitol, thiopental or both of them. These results suggest that, in cats, mannitol treatment is effective but not thiopental in preventing severe neurologic injury following complete global ischemia and the duration of complete ischemia should be far less than 15 minutes.

소아에서 외상성 질식 증후군에 대한 임상적 고찰

박승현,최승필,나병호,황주일,나석주,전해명,김세경 大韓應急醫學會 1996 대한응급의학회지 Vol.7 No.1

Traumatic asphyxia has often been described as a rare clinical syndrome characterized by cervicofacial cyanosis and edema, multiple petechiae, and subconjunctival hemorrhage after a severe crush injury of the thorax or upper part of the abdomen. The pathogenesis of traumatic asphyxia is that after compression of the chest or upper abdomen, intrathoracic pressure increased suddenly. Blood is forced out of the right atrium through the valveless innominate and jugular veins into the head and neck. This sudden increased thoracic pressure in small venules and capillaries causes rapid dilation and minute hemorrhages producing the petechiae often seen. Treatment is supportive and should be focused on the associated injuries. The prognosis for traumatic asphyxia is very good despite the alarming initial physical appearance. If the patient survives the initial insult, the prognosis is excellent. Neurologic sequale may be permernant. We experienced five case of the traumatic asphyxia, and its clinical and pathophysiologic features are discussed. Increased awareness of this syndrome by emergency physicians will result in better reporting ad understanding of its clinical implications.

자궁경부암종에서 신생혈관 형성과 증식세포 핵항원 및 Ki-67 표지지수의 예후적 의의

박찬필,박문향,이승연 대한병리학회 1997 Journal of Pathology and Translational Medicine Vol.31 No.1

케일녹즙이 고콜레스테롤식이 흰쥐의 혈청 및 간장의 지질성분에 미치는 영향

박필숙,강진순,정승용,박재옥,김행자 慶尙大學校 1987 論文集 Vol.26 No.1

체중 60~65㎏의 wistar계 숫쥐에게 cholesterol 무첨가식이에 물과 kale 녹즙을 각각 급여하여 혈청중 총 cholesterol 농도, HDL-cholesterol 농도, 간장중의 총 cholesterol 농도, 혈청 및 간장중의 TG와 PL의 농도 그리고 과산화지질 농도를 측정하여 다음과 같은 결과를 얻었다. 1) 혈청중 총 cholesterol 농도는 cholesterol 무첨가식이군인 1,2군산 cholesterol 첨가이식군인 3,4, 5군간에서 각각 유의성이 없었다. HDL-cholesterol 농도는 cholesterol 무첨가식이군인 1,2군과cholesterol 첨가이식이군인 3,4,5군에서 kale 녹즙을 급여한 군이 유의적으로 높았다. 2)혈청의 중성지방농도는 전 군간에 유의성은 없으나 물ㅇㄹ 급여한 군보다 kale 녹즙을급여한 군에 있어 약간 낮은 경향이었다. 혈청의 인지질 농도는 cholesterol 무첨가식이군 및 cholesterol 첨가이식군 모두 물을 급여한 군보다 kale 녹즙을 급여한 군이 유의적으로 높았다. 3)간장의 총 cholesterol 농도는 cholesterol 첨가식이군에 있어 물을 급여한 군이 약간 낮은 경향이었다. 4)간장의 중성지방농도는 cholesterol 무첨가식이군에 비해 cholesterol 첨가이식군인 3,4,5군이 유의적으로 높았으며, 이들 군간에 있어서는 kale 녹즙혼액군이 비교적 낮았다. 간장 인지질농도는 cholesterol 첨가식이군인 3,4,5군간에 있어 kale 녹즙과 녹즙혼액을 급여한 3,4,군이 비교적 낮았다. 5)혈청 및 간장의 과산화지질농도는 cholesterol 첨가이식군인 3,4,5군이 무첨가식이군에 비해 대체로 낮았으며 또한 kale녹즙과 녹즙혼액을 급여한 군에 있어 유의적으로 낮았다. 이상의 결과로 보아 kale 녹즙의 섭취는 혈청 HDL-cholesterol 농도의 상승효과가 있고 간장 중 cholesterol 및 인지질의 농도를 다소나마 저하시키며 또한 혈청및 간장의 과산화지질농도의 지하작요이 있으므로서 혈청 및 간장의 지질개선효과가 있는 것으로 사료된다. The effect of kale juice, Brassica oleracea, on lpid components in serum and liver were investigated by feeding male rats of wistar for four weeks. And the concentrations of T-cholesterol,phospholipid, triglyceride and lipid peroxide in serum and liver were analysed along with the HDL-cholesterol concentration of serum . The results were as follows: 1)There was not found ny significant influence on the concentration of T-cholesterol in serum, butsome of relatively higher inluence but some of relatively higher influence on the HDL-cholesterol concentration. 2)For the group fed with 100% kale juice the concentration of triglyceride was shown lower than for any other groups, while that of phospholipid was shown higher. 3)The concentration T-cholesterol in liver was shown a little lower for the kale juice groups. 4)The concentration of triglyceride in liver was remarkably decreased in the 100% kale juice group in comparison with other groups, whiel the phospholipid concentraion was not. 5)Lipid peroxide values in serum and liver were relatively decreased in the kale juice groups compared to other group.

W-3계 고도 불포화지방산의 혼합비율이 다른 유지가 지질대사에 미치는 영향

박필숙,김성희,정승용,김경숙,문순열 慶尙大學校 1988 論文集 Vol.27 No.1

혈청 콜레스테롤 및 중성지방의 농도와 밀접항 관계가 있으며 혈청 lipprotein의 대사에 영향을 미치는 w-3계의 지방산의 적정한 섭취 비율을 구명하기 위한 일환으로써 들깨기름과 라아드의 혼합비율을 달리하여 흰쥐에게 급이한 결과 다음과 같은 결과를 얻었다. 1. 혈청 총-콜레스테롤 농도는 들깨기름 7.5%+라아드2.5%를 급이한 2군에서 유의성있게 낮은 반면 10%라아드 급이군은 현저히 높았다. 2. 혈청 HDL-콜레스테롤은 3군과 4둔이 1군, 2군에 비해 낮았으며, 혈청 총-콜레스테롤에 대한 HDL-콜레스테롤 비율은 2,3,4군이 5군에 비해 높았다. 3. 혈청 중 인지질과 중성지방 농도는 5군에 비해 2,3군에서 현저하게 낮았다. 4. 간장 중 총-콜래스테롤 농도는 4군이 1,2,3군에 비해 비교적 낮았다. 5. 간장 중 인지질 농도는 각 군간에 유의적인 차이는 없었으나 중성지방의 농도는 5군보다 1,2군에서 유의성 있게 낮았다. 6. 시험유지의 지방산조성은 1군에서 lionenic acid가 dir 58%이었으며, 5군에서는 w-3계 PUFA가 0.1%, oleic acid가 47.5%이었다. 7. 혈청 lipoprotein pattern은 2군에서 HDL함량이 가장 많은 반면 LDL함량은 가장 적은 경향이었다. In order to investigate the suitable composition of the w-3 series polyunsaturated fatty acid on lipoprotein metabolism related with cholesterol and triglyceride concentrations in serum, it was examined the effects of w-3 PUFA contents of mixed fat on oil lipid metabolism in serum and liver of rats. The results were summerized as follow ; 1. The concentration of T-cholesterol in serum of rats was lowest in the group 2 (7.5% perilla oil + 2.5% lard). whereas it was highest in group 5 (10% lard). 2. The concentration of HDL-cholesterol in serum was lower in group 3, 4 than in group 1, 2 and the ratio of HDL-cholesterol to T-cholesterol were higher in group 2, 3, 4 than in group 5. 3. The concentrations of phospholipid and trigyceride in serum were significantly lower in group 2, 3 than in group 5. 4. The concentration of T-cholesterol in the liver was relatively lower in group 4 than in group 1, 2, 3. 5. Phospholipid values in the liver were not significantly different between groups, but the concentrations of triglyceride were remarkably lower in group 1, 2 than those of the group 5. 6. Linolenic acid in group 1 (10% perilla oil), in the fatty acid composition of test lipids used in the experiment. was as much as about 58%, but w-3 PUFA in group 5 was only 0.1% and oleic acid was as much as about 58%, but w-3 PUFA in group 5 was only 0.1% and oleic acid was as much as 47.5%. 7. HDL contents, in lipoprotein composition of serum were the most in group 2 (7.5% perilla oil+2.5% lard), while LDL contents were the least.

불연속 양단 자유보(미사일 모델)의 최대추진력에 관한 연구

박영필,강승천 연세대학교 산업기술연구소 1985 논문집 Vol.17 No.1

In this study, the dynamic stability of a launch vehicle with non-uniform mass and bending stiffness distributions is analyzed. The launch vechile is idealized as a free-free Euler-Bernoulli beam subjected to a controlled constant follower force. For the study, finite element model is formulated through the extended Hamilton's principle. The effects of the fuel consumption on the dynamic stability behavior of the launch vehicle is studied for various gains and locations of the direction control sensor.

全北經濟의 停滯性 克服에 관한 硏究

崔洛弼,朴承基,安津,朴泰植,李海經,金宣坤 全北大學校 1991 論文集 Vol.33 No.-

This research is to give light upon the cause of stagnated economy of Chonbuk province by comparing the interreginal conditions of economy mainly since 1960s which is the period between Japanese Colony and the development of this province, and to propose its measures to overcome it. Chonbuk region, Since its opening of Kunsan port, through the term of Japanese colony, had been degenerated into a relative area of stagnation compared with other areas due to land seizure and exploitation of agricultural products by Japan. Thus since the opening of port the structure of economy in Chonbuk region was deformed into that is typical monoculture from colonial exploitation. Of course, althrough the structure of Korean economy under Japanese colony was generally maimed through exploitative structure of economy, it was demonstrated by substantiation data, that Chonbuk region with its opulent exploited as a primary object area of exploitation by Japan. Deprived of its autogenous vitality and alienated even from "the age of development" for decades, economical structure of Chonbuk province couldn't but be still aggravated rather than improved. Eventurally the gap between present growing regions is more over deepening through the compound geometrical functions of various factors that restrict regional development of economy as analyzed above.