Purification and Characterization of a Xylanase from Bacillus sp . KK-1

Dae Joon Kim,Ki Hong Yoon Seung Ho Kim,Ki Haeng Cho,Bon Hong Min 생화학분자생물학회 1995 BMB Reports Vol.28 No.4

A thermophilic Bacillus sp. strain KK-1 isolated from soil produced an extracelluar xylanase. From the culture supernatant of Bacillus sp., the xylanase was purified to homogeneity by ammonium sulfate precipitation and DEAF-Sephadex A-50 chromatography. The molecular weight of the purified xylanase was estimated to be 45 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel permeation chromatography. The apparent K_m values for xylanase, using oat spelt xylan and birchwood xylan as substrates, were 7.1 ㎎/㎖ and 3.2 ㎎/㎖, and V_(max) values were 27.0 μ㏖·min^(-1)·㎎^(-1) and 29.0 μ㏖·min^(-1)·㎎^(-1), respectively. The xylanase hydrolyzed oat spelt xylan to mostly xylobiose, xytotriose, and xylose. The amino acid composition indicated that the xylanase contained high amounts of amino acid residues of glutamic acid and glutamine (Glx) and aspartic acid and asparagine (Asx).

정읍지역의 다양한 지표변위 인자들을 이용한 연약지반 리스크 평가

이효진(Lee, Hyo Jin),윤홍식(Yoon, Hong Sic),염민교(Yeom, Min Kyo),이준민(Lee, Joon Min) 한국측량학회 2018 한국측량학회 학술대회자료집 Vol.2018 No.4

연약지반의 지반침하는 고속철도의 열차 탈선, 심하게는 건물의 붕괴 등 대형재난을 야기할 우려가 있다. 그렇기 때문에 지반침하를 사전에 파악해 재난을 예방하기 위한 다양한 연구가 진행 중이다. 최근 광범위한 지역의 지표변위를 분석할 수 있는 InSAR(interferometric SAR)기법을 이용한 연구가 활발히 진행되고 있다. InSAR 기법 중 PS-InSAR(Permanent Scatterer Interferometric SAR)기법이란 SAR영상에서 긴밀도가 높은 고정산란체(PS:Permanent scatterer)를 이용하여 지반침하를 분석하는 기법으로 다른 InSAR 기법에 비해 대기에 의해 발생하는 오차가 적으며, 보다 정확한 지표 변위값을 도출할 수 있다. 또한 장기간에 걸친 시계열적 지표 변위 분석에 용이하다. 본 논문에서는 독일의 Terra SAR-X 위성 영상을 이용하여, 정읍지역의 지표변위를 분석하였다. 지반침하 데이터를 누적 지표 변위. MIN-MAX값, 그리고 최종침하량을 종합적으로 분석하여 보다 정확하게 결과를 도출하고자 하였다. 연구지역인 정읍시는 평야지역과 산지의 구분이 뚜렷하게 나타나는 지역이며, 연약지반인 평 야지역의 지반침하를 발견할 수 있었다. 연구 결과 정읍 신태인 지역은 최대 -11mm/year, 평균-3mm/year로 지반이 침하되고 있음을 확인할 수 있었다. 정읍지역의 지반침하 대비를 위해 다양한 노력들이 필요할 것으로 사료된다.

SMS를 이용한 경안천 하류구간의 하천흐름 분석

홍성민,정인균,이준우,김성준 한국지리정보학회 2004 한국지리정보학회지 Vol.7 No.1

본 연구는 경안천 하류구간(13.8㎞)을 대상으로 SMS의 RMA2 모형과 SES2D 모형을 이용하여 2002년 경안관측소의 최대홍수위를 기록한 8월 7일의 수리분석을 실시하였다. RMA2 모형을 적용하기 위하여 RIMGIS 데이터의 하천측량자료를 이용하여 입력자료로 활용하였으며, 생성돈 지형자료를 2차원 유한요소망으로 변환하여 모형을 수행하였다. 본 연구에서는 부정류 상태의 모의를 적용하였으며, 모형에 민감하게 반응하는 인자에 대해서는 선행 연구에서 실시한 민감도 분석결과를 고려하였고, 기타 입력인자는 통계연보 및 하천정비계획서를 참고하였다. 또한 대상하천에 SED2D 모형을 적용하여 홍수사상에 대한 하천의 하상 변동에 대한 모의를 실시하였으며, 대상 하천의 홍수시 유속은 0.05~3.85㎧의 분포로 상류와 하류의 큰 차이를 보였고, 하상변화는 0.0003~0.0135m가 발생하였다. The purpose of this study is to analyze various hydraulic characteristics using SMS(Surface Water Modeling System) RMA2 model. It is based on 2-D finite element method, River reaches (13.8㎞) from Gyeongan gauge station to the inlet of Paldang lake was selected. Finite element was made by RINGIS Data, and the analysis of river-changes was operated by unsteady flow. The sediment concentration and bed change was simulated using SED2D model. This River's velocity was distributed that 0.05~3.85㎧ and bed change was changed about 0.0003~0.0135m.

감조하천의 유로단면변화에 따른 유황해석

민병형,민일규,이승휘,박한기,박홍준 東亞大學校 建設技術硏究所 1997 硏究論文集 Vol.21 No.2

The subject of this study apply to KyungSangNamDo Sachun-Si Sachun-bay sea area for builds up a industrial complex, and inquire into the phenomenon in compared after with before reclamation. I investigated, how to remove these harmful influence and make a estimate that a water-level, flow direction and river-bed evolution by inflow of river, wave' s attack. So we have come to the conclusion as follow. 1. Water Level : After reclamation, the water-level is ascended, but the water-level is stable after dredging and changing water-course. 2. Velocity of Flow : After reclamation, back water broke out, the velocity of flow was faster after dredging and changing water-course, but the velocity of flow came to be stable in the downstream. 3. River-bed Evolution : After reclamation, the severe erosion occurred in the front of revetment, but after dredging and changing the water-course as the new stable water-course constituted, river bed evolution decreased and the flood wave flow smoothly. 4. After reclamation, the water-level remarkably increased, but the velocity of flow was nearly the same.

감조하천의 유로단면변화에 따른 유황해석

민병형,민일규,이승휘,박한기,박홍준 동아대학교 건설기술연구소 1997 硏究報告 Vol.21 No.2

The subject of this study apply to KyungSangNamDo Sachun-Si Sachun-bay sea area for builds up a industrial complex, and inquire into the .phenomenon in compared after with before reclamation. I investigated, how to remove these harmful influence and make a estimate that a water-level, flow direction and river-bed evolution by inflow of river, wave's attack. So we have come to the conclusion as follow. 1. Water Level After reclamation, the water-level is ascended, but the water-level is stable after dredging and changing water-course. 2. Velocity of Flow : After reclamation, back water broke out, the velocity of flow was faster after dredging and changing water-course, but the velocity of flow came to be stable in the downstream. 3. River-bed Evolution : After reclamation, the severe erosion occured in the front of revetment, but after d]:edging and changing the water-course as the new stable water-course constituted, river bed evolution decreased and the flood wave flow smoothly. 4. After reclamation, the water-level remarkablely increased, but the velocity of flow was nearly the same.

特性 부가 文法에 依한 類似 標的의 區分

김항준,홍순목,박민호 경북대학교 공과대학 1983 工大硏究誌 Vol.12 No.-

syntactic method is a general tool for pattern recognition but it's not good at distinguishing similar object. In this paper, we use attributed grammar to distinguish long nose plier from diagonal cutter, and good experimental results are obtained.

한국에서 유행한 Cholera균에 대한 Antibiotics sensitivity에 관한 연구

손준용,유재근,김영한,민창홍 國立保健硏究院 1970 국립보건연구원보 Vol.7 No.-

하수 2차 처리수 재이용을 위한 저압 및 중압 고도산화시스템의 성능평가

안규홍,안석,맹승규,김기팔,홍준석,정민우,권지향,Ahmed, Zubair 대한상하수도학회 2003 상하수도학회지 Vol.17 No.4

In the present study, the feasibility of UV/H₂O₂ systems was investigated using low and medium-pres sure lamps on biologically treated wastewater effluents for secondary effluent reclamation. Two types of UV lamps were used as the light sources (a 33W lowpressure mercury lamp and a 350-W mediumpressure mercury lamp). The results from these UV systems showed that the removal of organic compounds could be achieved in the contact time of longer than 30min (i.e., low UV doses). Efficiencies of color removal and disinfection were far better than those of organic matters measured as TOC, DOC and TCOD_(cr). In the low pressure lamp UV system, it has been found that DOC and color removals were 60.9 and 86.2% with 50㎎/L of H₂O₂ and contact times of 30 minute, respectively. Whereas, with the mediumpressure lamp UV system, TOC, DOC and color removal were 27.1, 5.6 and 95% with 14.3㎎/L of H₂O₂ and 14 minute of contact times, respectively. Both systems could be applied for the reclamation of secondary effluent treated with biological treatment processes.

Pseudomonas sp. HJ에 의한 Poly(Hydroxybutyric-Co-Hydroxyvaleric) Acid의 생산

손홍주,민관필,이상준 부산대학교 유전공학연구소 1995 분자생물학 연구보 Vol.11 No.-

하수처리장의 활성오니를 분리원으로 하여 수십종의 PHA 생산균을 분리하였다. 일반적으로 hydroxyvalerate monomer unit의 전구물질로 알려져 있지않은 glucose로부터 비교적 많은 PHA를 생산하는 균주를 공시균으로 선정하여 형태학적, 배양적, 생리학적 제 특성을 검토한 결과 Pseudomonas 속으로 동정되었다. 균체 생육을 위한 최적 배양온도 및 배양 pH는 각각 37℃와 7.0이었으며, 최적 탄소원으로서 glucose 1%, 최적 질소원으로서 (NH_4)_2SO_4 0.2%, K_2HPO_4 0.3%, KH_2PO_4 0.45%였다. 최적 PHA 생산조건을 조사하기 위하여 2단계 배양법을 이용하였다. PHA 생산은 배지성분중 NH_4, SO_4, Mg가 결핍되었을 때 향상되었고, 그중 NH_4의 결핍시 PHA 축적률과 HV monomer의 함량이 가장 높았다. C/N molar ratio 95.2에서 PHA 축적률이 가장 높았다. 공시균주 Pseucomonas sp. HJ는 alkane, alkanoic acid, alcohol을 탄소원으로 하여 PHB/HV를 생산하였다. PHA의 생산량과 HV monomer의 함량은 이용된 기질에 따라 다양하였으며, 특히 hexadecane와 propionate를 탄소원으로 하였을 때 PHA중의 HV monomer의 함량이 49∼74mol%로 매우 높았다. ^1H-NMR로서 공시균으로부터 분리정제된 PHA의 조성을 분석한 결과 PHB/HV copolymer임을 알 수 있었다. To produce PHA(polyhydroxyalkanoic acid) from microbe, dozens of microorganism have been screened from sewage sludge. Selected a strain HJ out of 50 strains of PHA producing bacteria has a capability of accumulating large amounts of PHB/HV copolymer when grown in batch culture with a single carbon source(glucose) that was not generally considered as precusor of hydroxyvalerate monomer unit. The strain HJ was identified as the genus Pseudomonas with respect to morphological, cultural, and biochemical characteristics. The optimal temperature and pH for cell growth were 37℃ and 7.0. The optimal medium compositions for cell growth were glucose 1% as a carbon source, (NH_4)_2SO_4 0.2%, as a nitrogen source, K_2HPO_4 0.3%, and KH_2PO_4 0.45%. To investigate the optimal condition for PHA production two-step cultivation method was employed. PHA production was induced by deficiency of NH^+_4, SO^2_4, Mg^+2. Besides carbon source, deficiency of all nutrients stimulated PHA productivity but deficiency of NH^+_4 stimulated the most HV monomer content. The highest PHA production was C/N molar ratio 95.2. Pseudomonas sp. HJ was also able to produc PHB/HV copolymer when cultivated on alkane, alkanoate, alcohol as carbon sources. Especially Pseudomonas sp. HJ was able to incorporate hydroxyvalerate into PHB/HV to level as high as from 49 to 74 mol% when grown in a medium containing hexadecane and propionate. The purified PHA was identified PHB/HV copolymer by ^1H-NMR analysis.

준 열원의 강도분포에 관한 연구

도시홍,정세민,배세환,성상기,박흥준 釜山水産大學校 1985 釜山水産大學 硏究報告 Vol.25 No.1

실험결과 다음과 같은 결론을 얻을 수 있었다. q=3,q=30일때 최대치의 확율은 각각 0.08, 및 0.029이었다. 이것은 Pearl과 Troup의 결과 (q=1.9106, q=5.6904 및 q=34.7657 일때 최대치의 확율은 각각 0.09, 0.06 및 0.02)와 아주 유사했으며, 관측시간의 증가에 따라 분포의 천이경향도 이론적 결과와 잘 일치 되었다. 또한 준열원에서 방출된 광자들이 공간내의 유한한 영역에서 고르게 분포되어 있을때 그 광원을 coherence time보다도 더 긴 시간동안 관찰한다면 그 관원을 gaussian으로 취급할 수 있으며, 광자에 대한 Bose-Einstein 통계의 적용이 타당함과 Mandel의 근사공식(q>>1, q<<1인 경우)이 잘 성립될 것임을 실험으로 예상할 수 있었다. Light emitted from a source fluctuates both spatially and temporally. The spatial fluctuations are due to the finite size of the source and the spatial incoherence of the photons emitted from various parts of the source. Temporal fluctuation are due to the wide blackbody spectrum emitted by such a source. It is a direct result of the Bose-Einstein statistics obeyed by photons. Actual measurements on the statistical behavior of thermal light are conducted by pseudothermal light source. In the experiment, we examined the probability density f(I) that the intensity I associated with one or pore coherence volumes is between I and I+dI and the deviation for q<1, which is given by G. Bedad et al. The derivation of f(1) for the case of more than one coherence volume has been given in an approximate form by Mandel and by Troup. As a result, we obtain p(n,T)=0.08 at q=3 and p(n,T)=0.029 at q=30. These are very similar to pearl and Troup's [p(n,T)=0.09 at q=1.9106, p(n,T)=0.06 at q=5. 690, p(n,T)=0.02 at q=34.7657]. So we can conclude that when we observerse pseudo-thermal source for a long time (compared with its coherent time), the source can be treated as a gaussian light source and Mandel's approximation is good fit for photon counting.