托裡消毒飮이 傷處治癒에 미치는 影響

殷載淳,黃甲洙 又石大學校 1992 論文集 Vol.14 No.-

These experiments were conducted to investigate the wound healing action of Taklee-Sodok-Eum(TSE) water ext. TSE extract did not affect on the paper disk granuloma formation and mouse paw edema induced by histamine, but inhibited on the leakage of evans blue into peritoneal cavity. Using proliferation of Balb/c 3t3 fibroblast cell line as an in vitro model of granulation tissue formation, the ability of TSE to stimulate cellualar proliferation of fibroblast cells was investigated. When the cells were seeded at 1 × 10 exp (4) cells/well, Balb/c 3T3 cells were reached to the late exponential phase at 3rd day. Under the conditions established above, TSE increased the proliferation of Balb/c 3T3 cells at concentration of 10^-6 g/ml. The treatment of 10^-6 g/ml of TSE did not influence on the DNA synthesis, but decreased the protein synthesis of the cells. The 10% serum from TSE treated mice(500㎎/㎏/day for 4 days) increased the proliferation of Balb/c 3T3 fibroblast markedly, and did not influence on the DNA synthesis and protein sythesis of the cells. The results suggest that wound healing in vivo will be agumented by the treatment of TSE. In addition, TSE may be of practical therapeutic use in wound healing.

複合生藥製劑의 止血作用및 摘出子官筋에 미치는 影響(第4) : 壽脾煎 및 歸脾양에 대하여 On Soo-Bi-Jeon and Kwi-Bi-Tang

殷載淳,李東熙,黃甲洙 又石大學校 1990 論文集 Vol.12 No.-

These experiments were conducted to investigate the pharmacological difference between Soo-Bi-Jeon and Kwi-Bi-Tang extract,, clinically used in gynecology, on the hemostasis and the contractile force of the isolated uterine muscle. For this purpose, the effects of the extracts on the bleeding time in mouse tail and prothrombin time in vitro were estimated. Forthermore, its activity on the isolated uterine muscle in rats were investigated. The results obtained were as following; The bleeding time and prothrombin time were significantly shortened compared with the control group in all samples, The uterotonic action produced by Kwi-Bi-Tang(Sample II), Sample III and IV (the same component crude drugs between not blocked by atropine (10^(-7)M) and cyproheptadine (10^(-7)M). but inlhibited by pretreatment of verapamil (10^(-7)M). On the oter hand, Soo-Bi-Jeon(Sample I) extract relaxed the uterine muscle.

甘草엑스의 抗炎 및 抗潰瘍作用에 대한 機轉

殷載淳,崔東晟,全焄,安文生 又石大學校 1989 論文集 Vol.11 No.-

Glycyrrhizae radix, commonly called licorice, has been used as anti-ulcerous and anti-inflammatory agents. It's main component, glycyrrhizin, is known to be effective as an anti-allergenic, anti-hepatic, and anti-viral agent, and shows steroid-like action. Histamine mediates and modulates inflammation through a variety of immuno-regulatory actions and stress-induced secretion of glucocorticoids(GC) is generally mediated by release of ACTH from the pituitary gland. This study was to investigate the mechanism of Glycyrrhizae Radix Ext. on anti-inflammatory and anti-ulcerous action. 1. The serum histamine contents was the most increased at the dose of 25㎍/㎏ LPS. injected rats(i.p), and the serum corticosterone was not significant. 2. Pretreatment of Glycyrrhizae radix Ext. (150㎎/㎏) was markedly decreasing the levels of serum histamine and corticosterone. 3. The serum histamine content was significantly increased in 120 minutes after LPS. injection. 4. The serum histamine content was decreased by pretreatment of cimetidine (H^2- receptor antagonist) 5㎎/㎏. These results were suggested that the effects of Glycyrrhizae Radix Ext, on anti-inflammatory and anti-ulcerous action was induced by its glucocorticoid-like action and inhibition of the histamine realese via H_2-receptor.

複合生藥製劑의 止血作用및 摘出 子宮筋에 미치는 影響(第3報) : 加減當歸補血湯

殷載淳,廉思俊,韓宗鉉 又石大學校 1989 論文集 Vol.11 No.-

These experiments were performed to investigate the effects of the KAGAM-DANGKWI-BOHYUL TANG(KDBT) extract, clinically used in gynecology, on the hemostatis and on the contractile force of the isolated uterine muscle. The bleeding time and the plasma prothrombin time test were employed as the means to estimate the hemostatic effect of KDBT extract, and the contractile effect of the extract was measured. The experimental results were summarized as followings; 1. In mice treated with KDBT extract the plasm prothrombin time and the bleeding time were remarkably shortened compared with the control group. 2. The contractile effect of KDBT extract on the isolated uterine muscle was not blocked by atropine and cyproheptadine. 3. The contractile effect of KDBT extract on the isolated uterine muscle was inhibited by the pretreatment with verapamil. From these experimental results, it is concluded that the hemostatic effect of KDBT extract might be attributed to the stimulation of prothrombin formation and the direct contraction of the uterine muscle through the increasing uptake of calcium ions into the muscle.

단삼이 마우스 T-lymphocyte 및 Macrophage에 미치는 영향

은재순,엄승렬,염정열,전훈 우석대학교 생명과학연구소 1998 생명과학연구소 논문집 Vol.2 No.-

The purpose of this research was to investigate the regulative action of water extract of Salviae miltiorrhizae Radix(SE) on thymocyte and macrophage in mice. The proliferation in thymocyte was tested using a colorimetric tetrazolium assay(MTT assay), The apoptosis and subpopulation in thymocyte were tested using a flow cytometry. Nitric oxide(No) production from mouse peritoneal macrophage was tested using a Griess reagents and the phagocytic activity was tested using a lucigenin chemiluminescence. SE did not affect the proliferation, apoptosis and subpopulation(CD4^+/CD8^+) in thymocyte in vivo, but enhanced the proliferation in thymocyte in vitro. The n-BuOH fraction and water fraction of SE suppressed the nitric oxide production and results suggest the SE does not affect the activity of thymocyte, but suppressed the activity of macrophage.

청피에 함유된 복강 파크로파지의 탐식작용 억제 성분

은재순,김대근,소준노,지옥표 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

The phagocytic activity of murine peritoneal macrophage was determined by lucigenin chemiluminescence and engulfment of fluorescein-conjugated E. coli particles. The activity-guided fractionation upon the methylenechloride fraction of Aurantii immaturi pericarpium led to the isolation of a flavonoid, isosinensetin, as a suppressive component of phagocytosis, lsosinensetin suppressed the lucigenin chemiluminescence and the engulfment of fluorescein-conjugated E. coli particles and enhanced the production of nitric oxide in murine peritoneal macrophage.

子官癌細胞(HeLa)에 대한 血府逐瘀湯과 數種 抗癌劑의 倂用投與 效果

殷載淳,蘇俊魯 又石大學校 1992 論文集 Vol.14 No.-

The studies were conducted to investigate the combined effects of Hyulbu Chuko Tang (HCT) and several anti-cancer drugs. The effects of HCT and several anti-cancer drugs on the proliferation of HeLa Colls, human cervical cell line, was estimated by MTT colorimetric assays. The 50% inhibitory concentrations of mitomycin C(MMC), cisplatin(CPT), mercaptopurine (MCP) and 5-fluorouracil(5-FU) on HeLa cell were 0.313, 0.565, 22.745 and 28.325/㎖, repectively. HCT extract inhibited the proliferation of HeLa cell at 10^-6-10^-3g/㎖. The inhibitory action of the MCP treated group on the proliferation of HeLa cell was increased by HCT and MCP. When the mice were treated by MMC, the number of leukocyte was decreased significantly at the 1st and 3rd day, but recovered at the 7th day. In the groups of MMC treated with HCT, the number of leukocytes was increased significantly than the group of MMC treated only at the 1st and 3rd day. The HCT extract increased the number of plaque forming cells(PFC), but the MMC treated group decreased the number of PFC. The combined treatment of MMC and HCT increased the number of PFC significantly than the MMC treated group. The HCT extract increased the proliferation of T cells, but the MMC treated group decreased the proliferation of T cells. The combined treatment of MMC and HCT increased the T cell proliferation significantly than MMC treated group.

L1210 세포증식에 대한 Glycyrrhizin의 억제작용 기전

殷載淳,徐龍勳,權鎭,柳東和,吳贊鎬,蘇俊魯,全焄,黃甲洙 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

The purpose of this research was to investigate the mechanism of inhibitory action of Glycyrrhizin(GZ) on the proliferation of mouse leukemia cell-line, L1210 cells. The cytotoxic activity was tested using a colorimetric tertrazolium assay(MTT assay), the apoptosis was tested using flow cytometry. Nitric oxide(NO) production form mouse peritoneal macrophage was tested using a Griess method and the phagocytosis of human polymorphonuclear cells was tested using a lucigenin chemiluminescence. GZ ingibited the proliferation of L1210, BALB/c 3T3 cells and mouse thymocytes at 50 ug/ml/ GZ did not affect nitric oxide production from mouse peritoneal macrophages in vitro, but ingibited nitric oxide production from lipopolysaccharide and y-interferon treated macrophages. Macrophages of GZ-administered mice accelerated NO production. The proliferation of L1210 cells apoptosis of L1210 cells were induced by co-culture with macrophage of GZ-administered mice. The apoptosis of L1210 cells were induced by co-culture with macrophage of GZ-administered mice. GZ increased the phagocytosis of human polymorphonuclear cells. These results suggest that GZ inhibit the proliferation of L1210 cells not only as a direct cytotoxic agent o tumor cells, but also by the enhancement of NO production and phagocytic activity.

靑皮가 마우스의 免疫細胞에 미치는 影響

殷載淳,吳贊鎬,鄭鉉雨 대한동의병리학회 1998 동의생리병리학회지 Vol.12 No.2

靑皮는 疏肝破氣 및 消積化滯하는 작용이 있는 약물로 대부분 氣滯로 인한 증상들에 사용되고 있지만 임상상 活血之劑와 配合하여 氣滯血瘀나 血瘀脇痛등에 應用되고 있다. 氣滯는 각종의 스트레스등으로 인하여 발생되어 血行을 不暢하게 하고, 심지어는 正氣까지도 손상시킨다. 正氣의 손상은 바로 免疫機能의 低下라고도 말할 수 있는데, 免疫이란 非自己로 인식된 異常細胞를 처리함으로써 個體의 恒常性을 維持하려는 현상을 말한다. 면역에 先天的 免疫과 T림프구 및 B림프구가 관여하는 特異性 免疫이 있고, 免疫作用에 관여하는 細胞로는 特異性 免疫에 관여하는 免疫細胞外에도 Macrophage, NK cell, 수지상 세포 및 랑게르한스 세포등을 들 수 있다. 뇌의 기능은 심장에서 방출되는 혈액의 공급에 의해 이루어지는데, 만약 혈액의 공급이 제대로 이루어지지 않으면 많은 질환들이 발생한다. 그 중 虛血性 腦疾患은 충분한 양의 혈액이 뇌를 공급되지 않거나 공급되고 있는 혈액내의 산소농도가 떨어져 발생하는 것으로써 임상적으로는 運動麻痺·知覺痲痺등 神經機能 消失등의 증상을 보이기 때문에 東醫學的으로는 이를 風病이라 하고, 風病은 대체적으로 陰虛나 血虛등으로도 발생할 수 있어 陰虛動風證 또는 血虛生風證등의 病證들이 있다. 그리하여 각종 氣滯로 인한 氣滯則血滯가 발생되거나 또는 正氣가 손상되어 氣虛則血虛하기 때문에 陰虛나 血虛등으로 인한 運動麻痺나 知覺麻痺가 발생한다. 이러한 이유등으로 本 硏究者들은 혈류장애 질환치료에 사용되는 韓藥材中 靑皮의 작용을 규명하는 硏究의 일환으로 靑皮가 면역기능에 어떠한 영향을 미치는가에 대하여 알아보기 위하여 동결건조된 靑皮를 마우스에 1일 1회씩 7일간 투여한 후 T-lymphcyte의 Apoptosis와 sub-population, 그리고 proliferation 및 Peritoneal macrophage의 nitric oxide 생성과 phagocytic activity를 관찰한 결과 DNA fragmentation과 sub-population에는 별다른 영향을 주지 않았지만 proliferation에 있어서는 증식이 촉진되었고, 복강 macrophage의 nitric oxide 생성과 phagocytic activity에 있어서는 NO는 감소하였으나 phagocytic activity는 현저히 증가하였다. 이는 靑皮가 NO 생성을 억제하여 phagocytic activity를 증가시킨 결과라 思料된다. 이상과 같은 實驗結果들을 통해서 볼 때 靑皮는 臨床的으로 각종 氣滯로 인한 正氣損傷과 血液循環障碍가 동시에 나타날 때에만 正氣를 보충해주는 役割을 할 수 있을 것으로 사료된다. The purpose of this research was to investigate effects of Citri Reticulatae Viride Pericarpium water extract(CRVP) on immunocytes in mice. The administration of CRVP did not affect apoptosis and sub-population of mouse T-lymphocytes, but increased the proliferation of T-lymphocytes. CRVP decreased nitric oxide production from mouse peritoneal macrophage, but increased a phagocytic activity of macrophages. These results suggest that CRVP does not affect T cell mediated immunity, but increases the phagocytic activity of peritoneal macrophages.

3, 4-Methylenedioxy Cinnamic Acid Amide 誘導體의 合成 및 藥理作用에 관한 硏究

殷載淳 又石大學校 1984 論文集 Vol.6 No.-

In order to search more active and less toxic compound in comparison with the central nervous depressant activity of piperine, 3,4-methylenedioxy cinnamic acid amides were synthesized by condensation of 3,4-methylenedioxy cinnamoyl chloride with alkyl or aryl amines, and N-acyl-3,4-methylenedioxy cinnamimides were synthesized by condesation of 3,4-methylenedioxy unsubstiuted amide with acyl chlorides. The bioassay results revealed that, among the compounds synthesized, 3,4-methylenedioxy cinnamoyl monoethylamide exhibited more potent protective activity against pentylenetetrazoleinduced convulsion, 3,4-methylenedioxy cinnamoyl unsubstituted amide, monoethylamide and anilide exhibited more potent protective activity against strychnine-induced convulsion, and all of the compunds except 3,4-methylenedioxycinnamoyl unsubstituted amide and monoethylamide showed less acute toxicity than that of piperine.