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단삼이 마우스 T-lymphocyte 및 Macrophage에 미치는 영향
은재순,엄승렬,염정열,전훈 우석대학교 생명과학연구소 1998 생명과학연구소 논문집 Vol.2 No.-
The purpose of this research was to investigate the regulative action of water extract of Salviae miltiorrhizae Radix(SE) on thymocyte and macrophage in mice. The proliferation in thymocyte was tested using a colorimetric tetrazolium assay(MTT assay), The apoptosis and subpopulation in thymocyte were tested using a flow cytometry. Nitric oxide(No) production from mouse peritoneal macrophage was tested using a Griess reagents and the phagocytic activity was tested using a lucigenin chemiluminescence. SE did not affect the proliferation, apoptosis and subpopulation(CD4^+/CD8^+) in thymocyte in vivo, but enhanced the proliferation in thymocyte in vitro. The n-BuOH fraction and water fraction of SE suppressed the nitric oxide production and results suggest the SE does not affect the activity of thymocyte, but suppressed the activity of macrophage.