식도운동질환과 위식도역류질환과의 연관성

홍성노 ( Hong Seong No ),이풍렬 ( Lee Pung Lyeol ),이준행 ( Lee Jun Haeng ),김영호 ( Kim Yeong Ho ),김재준 ( Kim Jae Jun ),백승운 ( Baeg Seung Un ),유병철 ( Yu Byeong Cheol ),이종철 ( Lee Jong Cheol ) 대한소화기학회 2003 대한소화기학회 추계학술대회 Vol.2003 No.-

<목적> 식도 운동의 장애는 위식도역류의 발생과 연관이 있으며, 식도운동질환과 위식도역류질환과의 연관성을 시사하는 여러 연구가 보고되고 있다. 이 중 새로이 분류된 비효과적식도운동(ineffective esophageal motility)은 위식도역류질환의 주요 식도내압검사 소견으로, 일부 연구에서는 위식도역류질환의 표지(marker)라고 주장되기도 하지만 논란이 되고 있으며, 이를 제외한 다른 식도운동질환에서 위식도역류질환의 빈도와 연관성을 살펴본 연

Klebsiella pneumoniae의 항균제 내성에 관한 연구

홍성노 ( Seong No Hong ) 대한임상검사과학회 1988 대한임상검사과학회지(KJCLS) Vol.20 No.1

Moraxella catarrhalis의 광역학적 비활성화

홍성노 ( Seong No Hong ),권필승 ( Pil Seung Kwon ),김대식 ( Dae Sik Kim ) 대한임상검사과학회 2007 대한임상검사과학회지(KJCLS) Vol.39 No.1

The aim of this study was to evaluate the bacterial effects of Moraxella catarrhalis in otitis media with effusion (OME) by photodynamic therapy (PDT). Bacterial suspensions (10000 CFU/mL) were prepared. The colony forming units (CFU) of Moraxella catarrhalis have been measured after an application of photogem plus 632 nm diode laser irradiation. One ml of the bacterial suspensions have been incubated in the dark for 3h with various concentrations of photogem (0.625~5.0 μg/mL) and then irradiated with 632 nm diode laser (15 J/cm2). After, the PDT Moraxella catarrhalis suspensions (50 μL) were inoculated on chocolate agar plate and cultured in the dark at 37℃, 5% CO2 condition for 18h. The colony forming units off the bacteria were measured. Also transmission electron microscopy (TEM) was employed to evaluate the effect of otitis media pathogens by PDT. The nucleus of Moraxella catarrhalis was stained using green fluorescent nucleic acid dye thiazole orange and the fluorescence intensity of the nucleus was measured by flow cytometry. The PDT was effective in killing Moraxella catarrhalis at the photogem dose of 5.0 μg/mL, respectively, As assessed by flow cytometry analysis the fluorescence intensity of the nucleus got lower after PDT. TEM result appeared to able to cause damage to the bacterial membranes. On the basis of these findings, bacterial photodynamic therapy with photogem can be considered to be a promising new therapeutic approach for OME.

최근 10년 동안 일개 대학병원 상처 배양에서 분리된 포도알균의 항생제 내성 변화 연구

홍성노 ( Seong-no Hong ),김준 ( Joon Kim ),성현호 ( Hyun-ho Sung ) 대한임상검사과학회 2016 대한임상검사과학회지(KJCLS) Vol.48 No.4

Staphylococcus aureus, which is generally susceptible to the involvement route in community, is mostly MSSA. However, CA-MRSA is recently increased. Abuse of antibiotics and glycopeptides may increase VISA and VRSA. This study was conducted to investigate the changes on the antibiotic resistance prevalence and antibiotic susceptibility patterns of Staphylococcus aureus isolated from the wound of patients in a university hospital for the past 10 years. This study showed that antibiotic resistance was higher in males than in females; moreover, the antibiotic resistance rates increased with age. The resistance rate for penicillin, oxacillin, erythromycin, gentamicin, clindamycin, tetracycline, ciprofloxacin, fusidic acid, trimethoprim/sulfamethoxazole, clindamycin, and rifampicin was, respectively, 97.7%, 60.5%, 57.4%, 48.8%, 41.1%, 44.2%, 44.2%, 14.7%, 13.2% and 3.9% in 2006. The resistance rate for penicillin, oxacillin, erythromycin, gentamicin, clindamycin, tetracycline, ciprofloxacin, fusidic acid, trimethoprim/sulfamethoxazole, fusidic acid, clindamycin, and rifampicin was, respectively 95.9%, 62.6%, 55.7%, 28.6%, 50.3%, 34.7%, 38.8%, 34.0%, 2.7% and 8.2% in 2016. Vancomycin, linezolid, quinupristin/dalfopristin, and teicoplanin exhibited 100% in antibiotic susceptibility. In particular, fusidic acid resistance was increased by 19.3% in 2016. Compared with 2006, the decreased point, 12.4% was susceptible and was statistically significant. Therefore, this study suggests that periodic review and understanding of microbial and antibiotic changes should continue to investigate appropriate antibiotic susceptibility.

우수논문학술상 논문게재 : Moraxella catarrhalis의 광역학적 비활성화 Photodynamic Inactivation of Moraxella catarrhalis

홍성노 ( Seong No Hong ),권필승 ( Pil Seung Kwon ),김대식 ( Dae Sik Kim ) 대한임상병리사협회 2008 임상미생물검사학회 발표자료집 Vol.2008 No.-

The aim of this study was to evaluate a bacterial effects of Moraxella catarrhalis in otitis media with effusion (OME) by photodynamic therapy (PDT). Bacterial suspensions (10000 CFU/mL) have been prepared. The colony forming units (CFU) of Moraxella catarrhalis have been measured after an application of photogem plus 632 nm diode laser irradiation. One ml of the bacterial suspensions have been incubated in the dark for 3h, with various concentrations of photogem (0.625~5.0 μg/mL) and then irradiated with 632 nm diode laser (15 J/cm2). After the PDT Moraxella catarrhalis suspensions (50 μL) were inoculated on chocolate agar plate and cultured in the dark at 37 ℃, 5% CO2 condition for 18h. The CFU of the bacteria have been measured. Also transmission electron microscopy (TEM) was employed to evaluate the effect of otitis media pathogens by PDT. The nucleus of Moraxella catarrhalis stained using green fluorescent nucleic acid dye thiazole orange. And The fluorescence intensity of nucleus measured by flow cytometry. The PDT was effective in killing Moraxella catarrhalis at the photogem dose of 5.0 μg/mL, respectively. As assessed by flow cytometry analysis get lower intensity after PDT. TEM result was appeared to able to cause damage to the bacterial membranes. On the basis of these findings, bacterial photodynamic therapy with photogem can be considered to be a promising new therapeutic approach for OME

Differences in the Antibiotic Resistance Pattern of Staphylococcus aureus Isolated by Clinical Specimens in a University Hospital in South Korea

홍성노,김준,성현호,Hong, Seong-No,Kim, Joon,Sung, Hyun-Ho Korean Society for Clinical Laboratory Science 2018 대한임상검사과학회지(KJCLS) Vol.50 No.2

Information on the prevalence of S. aureus and the current antimicrobial resistance profile is necessary in selecting the appropriate treatment of S. aureus infections in any part of the world. This study examined the frequency and antibiotic resistance list of S. aureus isolates obtained from clinical specimens at one hospital in Korea. A total of 1,746 gram positive cocci collected were identified as S. aureus. S. aureus isolates were obtained from different samples including sputum (N=565; 32.4%), endotracheal aspirate (358; 20.5%), wounds (329; 18.8%), blood (137; 7.8%), urine (67; 3.8%), and pus (59; 3.4%). All 1,545 S. aureus (100%) strains screened from sputum (565; 36.6%), endotracheal aspirate (388; 25.1%), wounds (329; 21.3%), blood (137; 8.9%), urine (67; 4.3%), and pus (59; 3.8%) were sensitive to glycopeptide (vancomycin, teicoplanin), oxazolidinone (linezolid) and stretogramin (quinupristin/dalfopristin). The prevalence of resistant S. aureus was significantly (P<0.01) lower in urine, blood, pus, wounds, and sputum than in endotracheal aspirates. As a result, there was a significant difference in the antibiotic resistance of S. aureus according to the clinical specimens. 황색포도상구균의 유병률에 대한 정보와 항생제 내성 리스트는 전 세계 어느 곳에서나 황색포도상구균 감염의 적절한 치료 방법을 선택하는 데 필요하다. 이 연구는 한국의 어느 병원 임상 검체에서 얻은 황색포도상구균 균주의 빈도와 항생제 저항성을 조사하기 위하여 수행하였다. 그람 양성구균 1,746 개의 균은 S. aureus으로 확인되었다. S. aureus은 객담(N=565, 32.4%), 기관내 흡인(358, 20.5%), 상처(329, 18.8%), 혈액(137명, 7.8 %), 고름(59 %, 3.4 %)이었다. 객담, 기관 내 흡인, 상처, 혈액, 소변 및 고름에서 스크리닝 된 1,282개의 S. aureus 균주 모두 글리코 펩타이드 옥시 졸리 디논(리네 졸리드) 및 스트렙토그라민(퀴뉴프리스틴/달포프리스틴)에서 모두 민감성이 나타났다. 황색 포도상 구균은 기관 내 흡인과 비교하면 소변, 혈액, 고름, 상처 및 객담에서 내성이 유의하게 낮았으며(P<0.01), 기관내 흡인 검체에서 분리 된 황색포도상 구균의 경우 특히 내성의 빈도가 높았다. 결과적으로, 임상 표본에 따라 황색 포도상 구균의 항생제 저항성에는 상당한 차이가 있었다.

복합재료 섬유흡착제를 이용한 해수로부터 우라늄 분리에 관한 연구(2)(흡-탈착 특성)

황택성,박정기,홍성권,신현택,노영창,Hwang, Taek-Seong,Park, Jeong-Gi,Hong, Seong-Gwon,Sin, Hyeon-Taek,No, Yeong-Chang 한국재료학회 1996 한국재료학회지 Vol.6 No.8

아미드옥심기와 복합재료 섬유흡착제를 제조하였고 해수로부터 우라늄이온의 분리 특성을 조사하였다. 흡착량은 흡착시간이 증가함에 따라 증가하였고 An:TEGMA:DVB의 몰비가 1:0.1:0.003인 수지가 pH 8 부근에서 최대 흡착능을 나타내었다. 또한 흡착량은 CFA에 첨가한 흡착제의 양이 증가함에 따라 증가하였으며 1시간 까지 선형적으로 증가하였고, $25^{\circ}C$에서 최대흡착량을 나타내었다. 한편 Ca, Mg 이온은 흡-탈착 cycle이 반복될수록 증가하였으며 그양은 각각 0.3, 0.9mmole/g-Ads로 우라늄 이온의 그것보다 매우 낮았다. 흡착된 우라늄 이온의 탈착은 흡착제의 종류에 관계없이 약 30분 이내에 거의 100% 탈착되었다. The composite fiber adsorbents containing amidoxime group were prepared and separation properties of uranium ion from seawater were investigated. The amount of uranium adsorption was increased with an increase in adsorption time. When the mole ratio of monomer and comonomer, such as acrylonitrile (AN), tetraethyleneglycol dimethacrylate(TEGMA), and divinylbenzene (DVB), were 1 :0. 1 :0.003, this resin showed the maximum adsorption ability for uranium at a level of pH 8. The amount of uranium adsorption was also increased linearly to one hour with an increase in the content of adsorbent which was added in the composite fiber adsorbents(CFA). The maximum adsorption for uranium of CF A showed at $25^{\circ}C$. Hence, the adsorption ability of CF A for calcium and magnecium ions were increased gradually by the recycling of adsorption and disorption, the adsorption content of their on were 0.3, 0.9mmole/g-adsorbents, respectly. It also showed that the adsorption contents of Ca and \1g ions were much lower than them of uranium. The desorption of uranium on the CF A was carried out , bout 100% within 30min, and the desorption rate of various CF A were equalled.

붕장어회를 먹고 Anisakis에 감염된 증례의 검토

임승렬 ( Seung Ryeol Lim ),홍성노 ( Seong No Hong ),김대식 ( Dae Sik Kim ) 대한임상검사과학회 2003 대한임상검사과학회지(KJCLS) Vol.35 No.2

Anisakis is nematode that is parasitic on fishes. Anisakiasis is a parasitic disease in which anisakis invades the gastroenteric tube of human body for reproduction from fish and it becomes an acute or chronic disease. In the Netherlands in 1960, van Thiel found eleven patients who had a chronic stomachache after eating herring that was not cooked well. Reports of these symptoms increased continuously. A 48 years old man became infected after he ate sliced fish of Astroconger myriaster in May, 2002. He appeared with a violent stomachache. We removed four worms. These worms were inspected for Anisakid larva and worms were found by an endoscopy in internal digestive organ department. A removed larva turned out to be "A nematode worm, consistent with anisakiasis" by histopathological examination, that is anisakiasis disease. Recent changes of eating habits show that people enjoy eating sliced raw fish. This produces much higher probabilities of being infected with Anisakis. Reproduction of a sliced raw fish of Astroconger myriaster requires special caution as a result of the investigation of the number of instances of infection after eating a sliced raw fish of Astroconger myriaster.

임상검체에서 분려한 ATB32C를 이용한 Trichosporon 균속의 새로운 species 동정

김봉철 ( Bong Cheol Kim ),홍성노 ( Seong No Hong ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3

Trichosporon species are a medically important genus that includes the causative agents of both deep-seated and superficial infections. In the past investigators had considered Trichosporon beigelii to be the asexual state white piedra. πley placed 깐ichosporon cutaneum as a later synonym of T. beigelii and listed τ beigelii as the type species of the genus Trichosporon. πle caustive agent of trichosporonosis was belived to be τ cutaneum (same as T. beigelii), but recent taxonomic research has indicated that six species, T. αsahii, I αsteroides, T. cutaneum, T. inkin, T. mucoides, and T. ovoides, are associated with the infection. A few reports have proposed that T. beigelii is a pathogen of onychomycosis in Korea. In the present study, we describe a yeast biochemical test of ID 32C(bioMerieux, France) approach for detection of all species in the genus Trichopsoron including the causative agent of onychomycosis. The strains used in this study are 58 strains isolated from patients of tertiary Hospital in Seoul. T. αsahii, T. mucoides, and T. inkin are 82.8%, 13.8%, and 3.4% respectively. The correct name for the main agent of onychomycosis is T. αsahii. πle name T. beigelii is considered as of doubtful identity. We suggest that T. asahii might be a coomon pathogen of onychomycosis in Korea. We contructed a readily used and accurate identification system for all of the species in the genus 깐ichosporon, including the six medically relevant species, based on additional analysis of biochemical test and slide culture.

말단비대증 환자의 혈중 프롤락틴 수치와 면역조직화학염색과의 상관관계

강보현 ( Bo Hyun Kang ),정인경 ( In Kyung Jeong ),조덕신 ( Duck Shin Cho ),강한욱 ( Han Wook Kang ),김형훈 ( Hyung Hoon Kim ),김범진 ( Beom Jin Kim ),민태훈 ( Tae Hoon Min ),손준성 ( Jun Seong Son ),홍성노 ( Sung No Hong ),이춘영 대한내과학회 2003 대한내과학회지 Vol.64 No.2

목적: 말단비대증은 대부분 성장호르몬 분비 뇌하수체 선종에 의한 것으로 35%에서 고프롤락틴혈증이 동반된다고 알려져 있다. 저자들은 말단비대증 환자의 뇌하수체 조직의 면역조직화학염색 소견과 혈중 호르몬 수치를 비교하여 이들간에 어떠한 관계가 있는지 알아보고자 하였다. 방법: 1998년 8월부터 2001년 5월까지 말단비대증 진단 하에 뇌하수체 종양 제거수술을 받은 환자 28명(남자:여자=18:10, 평균연령: 41세)을 대상으로 하였다. 혈중 기저 성장 Background: Acromegaly occurs by excessive secretion of growth hormone and more than 99% of cases are caused by a growth hormone-secreting pituitary adenoma. Pituitary adenomas expressing multiple immunoreactivities are common. We assumed that the pituita