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      • KCI등재

        Photodynamic Therapy for Methicillin-resistant Staphylococcus aureus with High-level Mupirocin Resistance using 630 ㎚ Light-emitting Diode

        Pil Seung Kwon(권필승),Jin-Kyung Kim 대한의생명과학회 2010 Biomedical Science Letters Vol.16 No.4

        This study aims to evaluate the effect of Photodynamic Therapy (PDT) against methicillin-resistant Staphylococcus aureus with high-level mupirocin resistance (Hi-Mup MRSA). To examine the antimicrobial effect of photogemmediated PDT against Hi-Mup MRSA, CFU quantifications, bacteria cell viability tests, and disk diffusion antimicrobial susceptibility tests were evaluated. In addition, one of PDT mechanisms was investigated by accumulating photogem (10 ㎍/㎖) in Hi-Mup MRSA. Photogem-mediated PDT properly inhibited the colony formation of Hi-Mup MRSA. Viable bacteria decreased greatly after a PDT application with photogem 10 ㎍/㎖ at energy density 15 J/㎠. The diameter of the inhibition zone around susceptible disks increased after PDT. In addition, we confirmed the accumulation of photogem in bacteria through fluorescent images. These results demonstrated that excellent photosensitization of Hi-Mup MRSA can be achieved using photogem with 630 nm LED irradiation. Thus, PDT may make survival Hi-Mup MRSA inactive.

      • KCI등재

        포토프린을 이용한 황색포도알균과 표피포도알균에 대한 광역학 치료의 항균효과

        권필승(Pil-Seung Kwon) 한국콘텐츠학회 2013 한국콘텐츠학회논문지 Vol.13 No.2

        광역학 치료는 미생물의 감염을 포함한 다양한 질병을 위한 대안치료로 권장된다. 이 연구의 목적은 포도알균속에 대한 직접제작한 630 nm 발광다이오드와 포토프린을 사용하여 광역학 치료의 항균효과를 평가하고자 하였다. 포토프린을 이용한 광역학 치료는 황색포도알균과 표피포도알균에 대한 항균효과를 집락형성수 정량법과 세균 생육능은 유세포분석기를 이용하여 시험하였다. 황색포도알균과 표피포도알균의 집락형성수 결과는 포토프린 50 ㎍/㎖와 630 nm 발광다이오드로 에너지밀도 18 J/㎠ 조건으로 광역학 치료 적용 후 각각 평균 1 cfu/ml와 평균16 cfu/ml이다. 추가로 광역학 치료 후 황색포도알균과 표피포도알균을 유세포분석기로 세포의 크기와 형광강도를 측정하였다. 황색포도알균과 표피포도알균의 세포크기는 광역학 치료 후 평균 8.96% 5.55%씩 각각 증가하였고, 세균의 죽은세포는 각각 39%와 61%로 증가하였다. 이 결과로 포토프린을 이용한 광역학 치료는 항균치료의 방법으로 효과적인 방법이 될 수 있음을 제의한다. Photodynamic therapy(PDT) has been recommended as an alternative therapy for various diseases including microbial infection. The aim of the present study is to evaluate the antimicrobial effect of PDT using a photofrin and home made 630 nm Light emitting diode(LED) against Staphylococci. To examine the antimicrobial effect of photofrin-mediated PDT against Staphylococcus aureus and Staphylococcus epidermidis colony forming units(CFU) quantification, and bacterial viability using flow cytometry were formed. The CFU quantification results of S. aureus and S. epidermidis were 1 cfu/ml and 16 cfu/ml of average, respectively, after PDT application with photofrin of 50 ㎍/㎖ and 630 nm LED and energy density of 18 J/㎠. In addition, S. aureus and S. epidermidis isolates yielded forward-scatter (FSC) and fluorescence intensity (FI) differences on flow cytometry (FCM) after PDT. S. aureus and S. epidermidis cell size(FSC) increased 8.96% and 5.55% respectively, after PDT. Also the numbers of dead cell of S. aureus and S. epidermidis were a 39% and 61% incerased. These results suggest that photofrinmediated PDT can be an effective alternative treatment for antibacterial therapy.

      • KCI등재후보

        장내세균 5종의 Endotoxin 정량 비교

        권필승 ( Pil Seung Kwon ) 대한임상검사과학회 2016 대한임상검사과학회지(KJCLS) Vol.48 No.2

        세균내독소는 lipopolysaccharide (LPS)라고 불리우며, 그람음성 세균의 세포벽에 존재하는 물질로 어느 생체재료나 액체에 존재할 수 있다. 세균내독소가 혈액으로 침투되면 발열 및 염증을 유발시킨다. 본 연구에서는 장내 그람음성세균인 Escherichia coli O157:H7, Klebsiella oxytoca, Salmonella Typhi, Shigella sonnei, Morganella morganii의 LPS 양을 비교하려 하였다. 각 세균은 배양한 후 1.5×108 CFU/mL 농도로 희석하여 동일한 농도로 하였으며 실험의 정당성을 위하여 LAL시약의 표시감도확인시험을 진행하였다. 10 fold dilution 후 1차 시험을 하여 각 균의 반응end point를 확인하고 바로 윗단계 희석배수 사이에서 2 fold dilution하여 균의 최종 반응 end point를 확인하였다. 결과적으로 E. coli O157은 75∼37.5 CFU/mL, K. oxytoca는 37.5∼18.75 CFU/mL, M. morganii와 S. Typhi는 3.75∼1.875 CFU/mL, S. sonnei는 7.5∼3.75 CFU/mL에서 0.015 EU/mL 이상의 endotoxin이 검출되었다. 최종적으로 억제인자에 대한 확인시험을 진행하여 결과값을 보증하였다. 본 연구를 통하여 세균내독소에 대한 추가적인 연구가 지속되기를 기원한다. Endotoxin, also known as lipopolysaccharide (LPS) produced by the cell wall of gram negative bacteria can be present in any liquid or on any biomaterial. Endotoxin in blood can cause fever and inflammation. In this study, we compared bacterial endotoxin using Escherichia coli O157:H7, Klebsiella oxytoca, Salmonella Typhi, Shigella sonnei and Morganella morganii. Bacteria were cultured for use in the experiment, and diluted to 1.5×108 CFU/mL. A check marked sensitivity confirmatory test of the Limulus amebocyte lysate (LAL) reagent was performed to examine the validity. The end point reaction to each bacteria sample was confirmed with 10 fold dilution and then the final reaction end point was confirmed by 2 fold dilution between the dilution step and the upper dilution step. According to the results, in detection of endotoxins in more than 0.015 EU/mL, E. coli O157 was 75∼37.5 CFU/mL, K. oxytoca 37.5∼18.75 CFU/mL, M. morganii and S. Typhi 3.75∼1.875 CFU/mL, and S. sonnei 7.5∼3.75 CFU/mL. The resulting value was finally ensured by a confirmation test for the inhibitory factor. Based on this study, conduct of further research on bacterial endotoxin is encouraged.

      • KCI등재후보

        금식 여부에 따른 혈당치 분포와 당뇨병 선별을 위한 혈당조절지표의 평가

        권필승 ( Pil Seung Kwon ),임인수 ( Insoo Rheem ) 대한임상검사과학회 2016 대한임상검사과학회지(KJCLS) Vol.48 No.4

        This study analyzed the distribution of the blood glucose level according to the fasting status. Moreover, a relationship was analyzed between fasting blood glucose level and glycemic control indicators. A total of 707 outpatients, who visited Dankook University Hospital, were included and classified into either the fasting group and the non-fasting group. The mean blood glucose level of each group was calculated and analyzed by sex, age, and clinic. In addition, blood glucose, HbA1c, fructosamine, and 1,5-AG were measured in 153 fasting health check-up patients, and the correlation between the blood glucose level and glycemic control indicators was evaluated. Blood glucose averages between the two groups (non-fasting 111.9 vs. fasting 103.6 mg/dL) were different (p<0.05); and the mean difference was lower in women (4.8 mg/dL) than in men (12.2 mg/dL). A significant difference of the median glucose values among the age groups was only observed in the non-fasting group (Kruskal-Wallis test, p<0.01), and not in the fasting group. A 1,5-Anhydroglucitol was estimated to be significantly correlated with the fast blood glucose level in the range of the criteria of impaired fasting glucose (IFG). We presented an assessment of the distribution of blood glucose level in accordance with the fasting status among outpatients, and estimated that 1,5-anhydroglucitol was well correlated with the fasting blood glucose than fructosamine and HbA1c, through the analysis of results of health screening subjects. It is suggested that the use of glycemic indicators that reflect short-term blood glucose control can be used together with the blood glucose measurement in the screening of diabetes mellitus.

      • KCI등재후보

        라다클로린으로 매개된 광역학치료에 의한 백색 캔디다 바이오필름의 비활성

        권필승 ( Pil Seung Kwon ) 대한임상검사과학회 2015 대한임상검사과학회지(KJCLS) Vol.47 No.4

        이 연구의 목적은 임상 Candida albicans의 바이오필름억제을 위한 Rhadachlorin과 적색발광다이오드를 이용한 광역학치료의 체외적 효과를 평가하고자 하였다. Candida albicans 부유액을 9×10(8)CFU/mL을 5% 당이 함유된 YNB배지에 준비하였다. Candida albicans의 바이오필름은 96웰 플레이트에 100 uL씩 분주후 3시간 배양하고 상층액을 제거하였다. 각 웰에 50 μg/mL 부터 0.39 μg/mL 농도로 Rhadachlorin을 부착된 세포위에 처리하였다. 30분간 배양 후 빛을 30, 60, 90분을 630 nm 광원의 LED를 이용하여 조사하였고, 이때 각각의 에너지밀도는 14, 29, 43 J/cm2 이였다. 모든 상층액을 제거후에 건조시켰다. 부착된 세포를 safranin O로 염색하였다. 490 nm 파장으로 마이크로플레이트 리더로 흡광도를 측정하였다. 또한, 광감작제포화에 의해 Candida albicans의 형광시그널을 관찰하였다. 결론적으로 확연한 억제는 Rhadachlorin의 농도 50 μg/mL과 630 nm LED치료에서 Candida albicans의 바이오필름의 72.5%가 감소되었다. 또한 광감작제 Rhadachlorin은 Candida albicans의 30분에서 충분한 축적을 보였다. 전체적으로 이 결과들은 바이오필름형성의 억제는 Rhadachlorin의 농도에 의존되었다. 이 결과들은 Rhadachlorin과 630 nm LED의 광역학치료는 Candida albicans의 바이오필름 억제가 가능한 것으로 제의된다. The purpose of this study was to evaluate the in-vitro efficacy of PDT using red light emitting diode (LED) with Radachlorin for biofilm inhibition of clinical Candida albicans isolates. The suspensions containing C. albicans at 9×10(8)CFU/mL were prepared on yeast nitrogen base containing 5% glucose. The biofilm formation was grown for 3 h after seeding suspensions each 100 ul on a 96-well plate and then supernatant was discarded. Each well was treated with 0.39 μg/mL from 50 μg/mL concentrations of Radachlorin on adherent biofilm. After a 30-minute incubation, light was irradiated for 30, 60, or 90 minutes using the following light source of wavelength 630 nm LED, at energy densities of 14, 29, and 43 J/cm2. Afterwards, all supernatant was removed and dried. Adherent cells were stained with safranin O and dried. The cell viability was measured using a microplate reader at 490 nm. Also, a fluorescent signal on C. albicans was observed by saturation of a photosensitizer. In conclusion, a significant inhibition of 72.5% was observed to C. albicans on biofilm at the Radachlorin dose of 50 μg/mL with 630 nm LED. The Photosensitizer (Radachlorin) was adequate at 30 minuttes for C. albicans. Overall, the results showed that inhibition of biofilm formation was Radachlorine dose-dependent. The results suggest that PDT, using Radachlorin with 630 nm LED, is able to decrease biofilm formation of C. albicans.

      • KCI등재

        형광화학센서를 이용한 살아있는 세포 내에서의 중금속이온검출

        권필승,김진경,김종완,Kwon, Pil-Seung,Kim, Jin-Kyung,Kim, Jong-Wan 대한화학회 2010 대한화학회지 Vol.54 No.4

        세포내의 중금속이온의 형광검출은 유기분자화학과 세포생물학분야에서 높은 관심을 갖는다. 이 연구는 형광화학센서(FS)를 이용한 Hg$^{2+}$ 과 Zn$^{2+}$의 세포 내 검출을 목적으로 하였다. FS는 약한 형광을 나타내지만 Zn$^{2+}$와 결합 시에는 강한 방출 형광은 낸다. 2FS/Zn$^{2+}$의 형광증가는 FS-Hg$^{2+}$결합의 형성할 때 Hg$^{2+}$ 1당량만 추가에도 완전한 형광감소를 나타낼 수 있었다. 네가지의 세포주(LLC-MK2, Hela, HT29 and AMC-HN3)는 공촛점 현미경에 대한 형광이미지를 위하여 사용하였다. 세포생존능은 LLC-MK2 세포주에 FS, Zn$^{2+}$, FS-Zn$^{2+}$, Hg$^{2+}$의 처리 후에 평가하였다. FS의 세포독성능은 80%이상의 생존능을 보였다. 본 연구에서는 FS가 살아있는 세포내의 Hg$^{2+}$과 Zn$^{2+}$의 선택적 이미지를 검출할 수 있음을 보여주었다. The fluorescence detection of intracellular metal ions are high interest in the fields of organic molecular chemistry and cellular biology. This study was purposed to detection for mercury and zinc in the cell using fluorescent chemosensor (FS). FS exhibits a weak fluorescence, but emits strong fluorescence upon Zn$^{2+}$ complexation. The increased fluorescence of the 2FS/Zn$^{2+}$ can be quenched completely by addition of only 1 equiv of Hg$^{2+}$ with the formation of complex FS-Hg$^{2+}$. Four cell lines (LLC-MK2, Hela, HT29 and AMC-HN3) were used for fluorescence imaging by confocal microscope. The cell viability MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was evaluated after cell treatment of FS, Zn$^{2+}$, FS-Zn$^{2+}$, Hg$^{2+}$ on LLC-MK2 cell line. The cytotoxicity of FS was showed to viability over 80%. This study has shown that FS can be detected for selective imaging of Zn$^{2+}$ and Hg$^{2+}$ in living cells.

      • KCI등재

        블렌딩 에센션오일의 항균효과 증진

        권필승,김대중,박호,Kwon, Pil Seung,Kim, Dae-Jung,Park, Ho 대한임상검사과학회 2017 대한임상검사과학회지(KJCLS) Vol.49 No.3

        식물로부터 유래되는 휘발성 유기 화합물인 에센셜 오일은 인체에 약리효과를 나타낸다고 알려져 있다. 본 연구에서는 화농성 질환과 병독성 질환 비뇨기계통 감염에 나올 수 있는 병원성 세균 Staphylococcus aureus (그람양성알균), Escherichia coli (그람음성막대균), Pseudomonas aeruginosa (그람음성막대균, 극성단모균)을 대상으로 천연 허브 오일 중에 비교적 많이 알려져 있는 라벤더(lavender), 티트리(tea tree), 로즈마리(rosemary), 멜리사(melisa) 4종류의 단일 오일과 비교해, 티트리와 로즈마리, 멜리사를 블렌딩하였을 때 항균력이 상승되는 효과가 있는지 연구하였다. 그 결과 디스크 확산법에서 블렌딩 오일(티트리, 로즈마리, 멜리사)의 경우 단독의 허브오일보다 S. aureus에서18 mm, E. coli 에서 22 mm 와 P. aeruginosa는13 mm의 우수한 항균능력을 확인할 수 있었다. 뿐만 아니라 블렌딩 오일의 경우 최소억제농도(Minimum Inhibitory Concentrations, MIC) 분석에서 S. aureus, E. coli 에서는 0.3125%에서, P. aeruginosa는 1.25%에서 성장억제효과가 확인되었다. 따라서 본 실험에서는 블렌딩 오일이 Oxacillin과 로즈마리, 티트리보다 항균효과가 우수한 것으로 증명되어 단독의 허브오일보다 블렌딩 오일이 우수한 항균력을 나타나는 것을 확인할 수 있었다. 그러므로 허브의 블렌딩을 활용하면 다제 내성균에 대한 항균시너지효과를 발휘할 수 있음을 입증했을 뿐만 아니라 향후 천연보존제와 건강기능성 식품 및 화장품에 활용 가능성을 기대해본다. Essential oil from herb is known to exert pharmacological effects on the human body. In this study we investigated the antibacterial activity of 4 essential oils (teetree, rosemary, melisa, and lavender), as well as the blended mixture oil of teetree, rosemary, and melisa (TRM) on three bacteria, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Antibacterial analysis was performed using the standard disk diffusion method, and minimum inhibition concentration was determined by the broth microdilution method with different concentrations of essential oils (0.5, 1, 2 and 3 mg/mL). After incubation at $37^{\circ}C$ for 24 h, the antibacterial activity was assessed by measuring the zone of growth inhibition surrounding the disks. Herb oil with the inhibition zones showed varied values ranging from6 to 25 mm. However, the components of herb oil of TRM are as highly active as the teetree oil against pathogens, generating large inhibition zones for both gram negative and positive bacteria (13~22 mm and 8 mm inhibition zones). In the analysis for MIC, TRM showed growth-inhibitory effects at 0.0625% for S. aureus and E. coli, and 1.25% for P. aeruginosa. This result demonstrated that the anti-microbial activity of TRM was greater than a single herb oil, including oxacillin, rosemary, and teetrea. As a single herb oil, both rosemary and teetrea also had an anti-microbial effect by itself, and we can expect that the blended oil mixture may exert a synergistic effect against multidrug resistant bacteria, suggesting its future application in natural preservative agents for health food and cosmetics.

      • KCI등재후보

        흰쥐의 MIA 유발 무릎 뼈관절염에 대한 840 ㎚ LED의 효과

        제갈승주 ( Seung Joo Jekal ),권필승 ( Pil Seung Kwon ),김진경 ( Jin Kyung Kim ),이재형 ( Jae Hyoung Lee ) 대한물리의학회 2014 대한물리의학회지 Vol.9 No.2

        PURPOSE: The purpose of this study was to evaluate whether light-emitting diodes (LED) irradiation could be effective in a noninvasive, therapeutic device for the treatment of osteoarthritis(OA). METHODS: Twenty-four male Sprague-Dawley rats were divided into four groups: Vehicle control (saline); monosodium iodoacetate-injection (MIA); LED irradiation after MIA injection (MIA-LED); indomethacin-treatment after MIA injection (MIA-IMT). OA was induced by intra-articular injection of 3 ㎎ MIA through the patellar ligament of the right knee. Vehicle control rats were injected with an equivalent volume of saline. The LED was irradiated for 15 min/day for a week after 7 days of MIA treatment. To compare with the effect of LED irradiation, the indomethacin was administrated 20 ㎎/㎏ twice a week orally after 7 days of MIA treatment. Knee joints were removed and fixed overnight in 10% neutral buffered formalin and decalcified by EDTA for 2 week before being embedded in paraffin. The assessment of OA induction were monitored by knee movement and radiographic finding. Histologic analysis were performed following staining with hematoxylin and eosin, safranin O-fast green, or toluidine blue, picrosirius red, and histologic changes were scored according to a modified Mankin system. Apoptotic cell in tissue sections was detected using TUNEL method. RESULTS: Radiographic examination could not show the differences between the MIA-treated and the MIA-LED-treated rats. In the histologic analysis, however, LED irradiation prevented cartilage damage and subchondral bone destruction, and significantly reduced mononuclear inflammatory cell infiltration and pannus formation. LED irradiation also reduced apoptosis of cartilage cells, but it prevented apoptosis of infiltrated inflammatory cells in synovium. In addition, LED irradiation showed an increase of collagen production in the meniscus. CONCLUSION: These results suggest that the 840 ㎚ LED irradiation would be a suitable non-thermal phototherapy for the treatment of OA, as a cartilage protection and anti-inflammatory modality.

      • KCI등재

        희소방선균의 seaR 단백질 발현을 통한 기능 분석

        류재기,권필승,이형선,Ryu, Jae Ki,Kwon, Pil-Seung,Lee, Hyeong Seon 한국미생물학회 2015 미생물학회지 Vol.51 No.1

        방선균이 생산하는 이차대사산물은 자기조절인자(${\gamma}$-butyrolactone autoregulator)라고 불리는 저분자의 신호전달물질과 이에 특이적으로 결합하는 autoregulator receptor protein의 상호작용에 의해 조절되는 것으로 알려져 있다. 그러므로 non-host에 autoregulator receptor 혹은 pleiotropic regulator의 발현은 이차대사산물 혹은 새로운 대사화합물의 효율적인 생산을 유도할 것으로 기대된다. 희소방선균 Saccharopolyspora erythreae으로부터 receptor (seaR) 유전자의 기능을 연구하기 위해 다른 속의 균주인 Streptomyces coelicolor A3(2)로 seaR 유전자를 삽입하여 형질전환하였다. S. coelicolor A3(2)의 형질전환은 oriT, attP, $ermEp^*$과 seaR gene 단편을 가지고 있는 ${\Phi}C31$ 유래의 integration vector인 pEV615 (6.6 kb)를 이용하여 Escherichia coli ET12567/pUZ8002를 DNA 공여체로 이용한 접합전달법을 사용하여 확립하였다. seaR 유전자의 삽입 유무는 PCR방법으로 확인하였고, seaR 유전자의 전사 발현은 RT-PCR방법으로 확인하였다. S. coelicolor A3(2)의 경우 표현형 microarray 실험을 통하여 seaR 유전자의 발현에 따른 표현형의 변화를 확인하였다. 특히, 표현형 microarray 실험에 나타난 tetracycline 항생제 기질에 대하여 wild type이 transformant에 비해 빠르게 성장하는 것은 항균제 감수성 검사와 일치하였다. 이는 tetracycline 생합성 유전자 및 내성 유전자의 발현 억제에 따른 변화라고 예상할 수 있으며 이를 위하여 tetracycline 생합성 관련 유전자 및 내성 유전자의 발현 패턴 분석등과 같은 분자 수준에서의 연구가 필요할 것으로 생각된다. Secondary metabolism in actinomycetes has been known to be controlled by a small molecule, ${\gamma}$-butyrolactone autoregulator, the binding of which to each corresponding receptor leads to the regulation of the transcriptional expression of the secondary metabolites. We expected that expression of an autoregulator receptor or a pleiotropic regulator in a non-host was to be gained insight of effective production of new metabolic materials. In order to study the function of the receptor protein (seaR), which is isolated from Saccharopolyspora erythraea, we introduced the seaR gene to Streptomyces coelicolor A3(2) as host strains. An effective transformation procedure for S. coelicolor A3(2) was established based on transconjugation by Escherichia coli ET12567/pUZ8002 with a ${\varphi}C31$-derived integration vector, pSET152, which contained int, oriT, attP and $ermEp^*$ (erythromycin promotor). Therefore, the pEV615 was introduced into S. coelicolor A3(2) by conjugation and integrated at the attB locus in the chromosome of the recipients by the ${\varphi}C31$ integrase (int) function. Exconjugant of S. coelicolor A3(2) containing the seaR gene was confirmed by PCR and transcriptional expression of the seaR gene in the transformant was analyzed by RT-PCR. In case of S. coelicolor A3(2), a phenotype microarray was used to analyze the phenotype of transformant compared with wild type by seaR expression. After that, in order to confirm the accuracy of the results obtained from the phenotype microarray, an antimicrobial susceptibility test was carried out. This test indicated that sensitivity of the transformant was higher than wild type in tetracycline case. These results indicated that some biosynthesis genes or resistance genes for tetracycline biosynthesis in transformant might be repressed by seaR expression. Therefore, subsequent experiments, analysis of transcriptional pattern of genes for tetracycline production or resistance, are needed to confirm whether biosynthesis genes or resistance genes for tetracycline are repressed or not.

      • KCI등재

        황련해독탕의 항균활성 및 표준화 연구

        이대연,이영우,김완수,심선형,권필승,이인희,Lee, Dae-Yeon,Yi, Young-Woo,Kim, Wan-Su,Sim, Sun-Hyung,Kwon, Pil-Seung,Lee, In-Hee 대한임상검사과학회 2019 대한임상검사과학회지(KJCLS) Vol.51 No.2

        Hwangryunhaedok-tang (HT) is prescribed in oriental medicine to treat inflammation-related diseases. This study examined the anti-bacterial effect to select the index components of Traditional Chinese Medicine (TCM) HT. The disc diffusion method and minimum inhibition concentrations (MIC) tests were conducted to measure the antibacterial activity of HT. The strains were Staphylococcus aureus ATCC 25923 and Escherichia coli 23724. Berberine and geniposide were selected as the index components of HT and were analyzed quantitatively by high-performance liquid chromatography (HPLC). As a result, the inhibition ring was formed at $11.7{\pm}1.3mm$ and $8.7{\pm}0.7mm$ at HT 20 mg and 10 mg on S.aureus ATCC 25923, respectively; and $8.0{\pm}0.0mm$ at HT 20 mg on E.coli ATCC 23724. The MIC were 31.25 mg/mL in S.aureus ATCC 25923 and 125.0 mg/mL in E.coli ATCC 23724. In quantitative analysis for standardization, HT contained 16.55 mg/g of berberine and 81.85 mg/g of geniposide. Overall, when antibiotics are developed using natural products, they may reduce the side effects and inhibit resistant bacteria. If research on HT continuously proceeds, positive developments are expected to be made in oriental medicine as well as in various other industries. 황련해독탕은 한방산업에서 염증관련 질환에 다빈도로 사용되는 처방이다. 본 연구의 목적은 전통적 한약인 황련해독탕의 항균활성을 확인하고 지표성분을 선정하는 것이다. 황련해독탕의 항균활성을 측정하기 위하여 디스크 확산법과 최소억제농도법을 사용하였다. 사용균주는 Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 23724였으며 대조군으로 oxacillin과 ciprofloxacin을 이용하여 황련해독탕의 항균활성을 확인하였다. 결과적으로 디스크 확산법에서는 S.aureus ATCC 25923에서 황련해독탕 20 mg과 10 mg은 $11.7{\pm}1.3mm$, $8.7{\pm}0.7mm$의 억제환을 형성하였으며 E.coli ATCC 23724에서는 황련해독탕 20 mg이 $8.0{\pm}0.0mm$의 억제환을 형성하였다. 최소억제농도는 S.aureus ATCC 25923가 31.25 mg/mL, E.coli ATCC 23724 에서는 125.0 mg/mL였다. 표준화를 위한 HPLC 정량분석 결과, 황련해독탕은 berberine을 16.55 mg/g 함유하고 있었으며 geniposide를 81.85 mg/g 함유하고 있었다. 천연물을 이용하여 항생제를 개발할 경우에는 내성세균을 억제하고 부작용을 줄일 수 있다는 장점이 있다. 이러한 점을 착안하여 지속적으로 연구를 진행한다면 한방산업과 더불어 여러 산업에서의 발전을 기대할 수 있을 것이다.

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