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토양 서식 미생물을 이용한 자일렌(xylene) 분해특성 조사
최필권(Phil Kweon Choi),허평(Pyeung Heo),이상섭(Sang Seob Lee) 大韓環境工學會 2013 대한환경공학회지 Vol.35 No.6
본 연구의 목적은 유류로 오염된 토양으로부터 분리된 고효율 분해균주에 의한 xylene 분해특성을 조사하고 분해과정을 밝히는 것이다. P. putida BJ10에 의해 mineral salts medium (MSM)배지내에서 24시간 배양 후 제거율은 o, m, p-xylene각각 94, 90, 98%였으며 3% 이하의 대조군과 명확한 차이를 보였다. 또한 유류로 오염된 토양내에서의 9일 경과 후 xylene제거율은 유류 분해균주 주입구에서 66%였으며 무처리 일반토양에서 32%, 멸균 토양에서 8%로서 P. putida BJ10에 의한 유효성을 확인할 수 있었다. 또한 시간경과에 따른 대사산물을 분석한 결과 o-xylene의 분해 경로에서는 6시간 경과 후 3-methylcatechol, 24시간 경과 후 o-toluic acid 가 분해산물로서 검출되어 최종산물로서의 o-toluic acid 가 확인되었으며 중간산물의 변환과정은 기존 발표된 연구들과 다소 다른 결과를 나타내었다. The purpose of this study is to investigate the biodegradation characteristics of the xylene by BTEX-degrading bacteria, Pseudomonas putida BJ10, isolated from oil-contaminated soil and bio-degradation pathway of the xylene. The removal efficiencies of o, m, p-xylene in mineral salts medium (MSM) by P. putida BJ10 were 94, 90 and 98%, respectively for 24 hours. It shows clear difference compared with the control groups which were below 3%. The removal efficiencies of BTEX by P. putida BJ10 in gasoline- contaminated soil were 66% for 9 days. They were clearly distinguished from the control groups (control and sterilized soil) which were 32 and 8%. 3-methylcatechol and o-toluic acid were detected after 6 and 24 hours during the o-xylene biodegradation pathway. Therefore, we confirmed o-toluic acid as the final metabolite. And intermediate-products were somewhat different with previously published studies of the transformation pathway from o-xylene to 3-methylcatechol.
최필권 ( Phil-kweon Choi ),허평 ( Pyung Huh ),이경희 ( Kyong-hee Lee ),조덕희 ( Deok-hee Cho ),김창규 ( Chang-gyu Kim ),김태화 ( Tae-hwa Kim ) 한국환경분석학회 2018 환경분석과 독성보건 Vol.21 No.3
In this study, mineral springs were monitored to determine whether they are being contaminated by rainfall onto microorganisms. In addition, the sterilization efficiency of an ultraviolet sterilizer used in various mineral springs was investigated. It was observed that the incongruity rates of total coliforms, fecal coliforms, and heterotrophic plate counts were 64.8%, 20.1%, and 11.4% respectively. In July, when rainfall was high, the incongruity rates of microorganism-related characteristics were at their highest (76%), suggesting that changes in precipitation were directly related to incongruity rates of microorganism-related characteristics within mineral springs. A T-test result of 0.002 (p<0.05, significant, 2-tailed) between the clay contents of soil in the valley and those of a control soil suggested that the possibility of underground penetration of soil in the valley is higher than that of general soil. Results of the sterilization test showed that E. coli was inactivated at the time of contact with an ultraviolet intensity of 10,000±20 μW/cm2 for at least 3 seconds and no photorecovery was observed.