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허성,김창수,정용석 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.4
Apple trees are perennial plant and have a characteristic of requiring long period from seed germination to flowering and fruiting. Most Korean apple cultivars are derived from closely related varieties genetically; consequently, they are distributed into grafted seedlings at juvenile stage. Therefore, it is difficult for them to distinguish by morphological traits due to the high similarities without fruit characteristics. We have integrated the high-resolution melting (HRM) analysis with simple sequence repeat (SSR) marker genotyping to identify thirteen apple cultivars originated from South Korea. The patterns of temperature-shifted melting curves were investigated from HRM analysis. The SSR loci used in this study generated a unique melting curve profile for each cultivar allowing their comparison and classification. The combination of the allelic patterns obtained with the just three SSR markers was able to identify thirteen apple cultivars. These results suggest that SSR markers used in combination with HRM analysis can be a fast and accurate method for high-throughput cultivar identification and biodiversity assessment.
허성,최정민,Le Anh Tuan,은창호,김인중,정용석 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.2
Fruit trees are perennial plant to have a characteristic requiring a long juvenile period until fruit set in general. Developing to release a new fruit cultivar is a laborious and cost- and time-consuming work. Especially, citrus breeding is not always feasible due to its cross-incompatible, sterile, and polyembryonic characteristics. Therefore, mutagenesis has been widely applied to citrus breeding to increase mutation frequency and genetic variability enabling development of new genotypes as an alternative method. Recently, various tangor (Citrus unshiu× C. sinensis) cultivars were released through interspecific hybridization. Many attempts have been made to induce mutations in developing tangor cultivars, while their physiological effects have not been verified yet. To investigate the effect of gamma-irradiation exerted on tangor cultivar ‘Kanpei’, its scions were irradiated with 20, 40, 60, 80, and 100 Gy of gamma-rays. Then, we examined the SPAD values between the gamma- irradiated M0 plants comparing with control ‘Kanpei’ (0 Gy). Interestingly, we found that there are variations in SPAD values among different treatments. This implies that mutation breeding should consider different factors other than the target traits.
Rapid real-time detection method of ACLSV and ASSVd for apple quarantine field
허성,정용석 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.2
Apples are produced using a high-density plantation system using M.9 rootstock in Korea. However, the virus infection rate of apples tends to be high because the rootstocks produced from virus-infected mother stocks were widely used and vegeta�tive propagation based on the grafting is common with scion cultivars. The most efective way to control plant virus and viroid depends on conducting a massively rapid and precise diagnostic inspection. Therefore, this study aims to develop a real-time PCR assay that can be precisely and inexpensively tested for ACLSV and ASSVd. To expand the detection range, we developed a singleplex real-time PCR method targeting the conserved region of ACLSV and ASSVd, respectively. The detection methods using two kinds of fuorescent chemical dye, SYBR Green I and TaqMan probe, have been developed in this study. Comparing these methods developed with two dyes, the assay using SYBR Green I was efcient for detection as much as the method using TaqMan probe and faster. The feld test demonstrated real-time detecting methods developed using SYBR Green I dye were applicable to reliable diagnosis and quantifcation for virus and viroid in quarantine felds.