Glycoportein IIb/IIIa와 칼슘동원의 조절을 통한 Steppogenin의 혈소판활성 억제효과

신정해,하주연,권혁우 한국생약학회 2020 생약학회지 Vol.51 No.2

The extract of Cudrania tricuspidata is used in ethnomedicine throughout Eastern Asia in China, Korea and Japan. In Korean traditional medicine, Cudrania tricuspidata has been used to treat eczema, mumps, tuberculosis, contusions, insomnia and acute arthritis. In addition, it has been reported that root extract of Cudrania tricuspidata has anti-platelet effects. Therefore, we investigated which compound in Cudrania tricuspidata has inhibitory effect on platelet aggregation. In this study, we tried to explain the inhibitory mechanism of steppogenin from Cudrania tricuspidata on human platelet aggregation. Collagen-induced human platelet aggregation and [Ca2+]i mobilization were dose-dependently inhibited by steppogenin and we determined the inhibition by steppogenin is due to the down regulation of extracellular-signal-regulated kinase(ERK) and inositol-1,4,5-triphosphate receptor type I(IP3RI) phosphorylation. In addition, steppogenin inhibited collagen-induced fibronectin adhesion to aIIb/b3 and thromboxane A2 generation. Thus, in the present study, steppogenin showed an inhibitory effect on human platelet aggregation, suggesting its potential use for preventing platelet-induced cardiovascular disease.

계피, 계지 및 계심의 규격 및 품질평가법에 관한 연구

장승엽,강신정,이종필,박상용,신지현,정영자,박진영,하광원,박종희,박정일 식품의약품안전청 1998 식품의약품안전청 연보 Vol.2 No.-

계피, 계지와 계심은 해열, 진통의 목적으로 사용되는 생약애다. 본 연구에서 cinnamaldehyde, cinnamic.acid,총탄닌 띤 정유에 대하여 분석하였다. 그 결과는, 1) 이 들 생약의 각 met'hanoi추출물과 ether추출물의 핀PLC 크로마토그램 양상은 서로 비슷하였다. 2) Methanol추출물과 ether추출물의 cinBamic acid 함량은 거의 비슷하였으나, cinnamaldehyde 함량은 ether추출물에서 더 많이 검출되었다. 3) 이 들 생약의 정운추출물을 GC와 GC/MSD로 분석한 결과 cinnamaldehyde가 주성분으로 검토되었다.또한 methanol추출물과 methanol에 웅해한 cinnamaldehyde 표준액에셔 rnethoxycinnamic acid로 추전되는 성분으로 변화하는 것을 알 수 있었으며, 시간이 지남에 따라 이 성분의 함량이 점점 증가함을 관찰할 수 있었다. 4) Folia-Denis법에 의하여 계피와 계지에서 총탄닌을 둔석한 결과 계피에서는 2.4~ 13.2%, 계지에서는 5.1-10.8%로 검출되었다. 중국에서 수입되는 계피에서는 7.2~9.1%, 베트남에서 수입되는 4.4~9.5%로 검출되었다. Cinuarnomi Cortex, Cinnamomi Ramulus and Cassino Cortex Interior are used as an antipyretic, anodyne eft. The contents of cinnamald☞hyde, cinnamic acid and total tannins as well as theamount of essential oil were assayed in this study. The results were as follows ; 1) The HPLC chro.matograms of methanol extract from these herb drugs were very similar to those ofether extract. 2) The contents of cinnamic acid between methanol and ether extract were almost same, but the con-tents of cinnamaldehyde in ether extract were higher than 1,hose in methanol extract. 3) eC and GC/MSD analyses of essential oil derived from these herb drugs revealed thatcinnamaldehyde was the main component and a unknown peak presumed to be rnethoxycinnamic acidwas found from methanol stock solutions of cinnamaldehyde and these herb drugs. The amount of thiscomponent was increased according to the time elapsed. 4) Total tannin contents assayed by Folin-Denis method in Cinnamomi Cortex and CinnamomiRamulus were determined as 2.4~13.2% and 5.1 ~10.8%, respectively, and total tannin contents ofCinnamomi Cortex from China and Vietnam were also determined as 7.2~9.1% and 4.4~9.5%,respectifely.

CsI(CO$_3$) 섬광 단결정 육성 및 섬광 특성 연구

강상준,김홍주,하신정,김민정,소중호,Gul Rooh,강희동,도시홍,김성환 한국물리학회 2008 새물리 Vol.57 No.1

It is important to develop new crystals that have good scintillation properties for use in high energy physics, nuclear physics, and medical imaging systems. We grew CsI(CO$_3$) crystals doped with 0.01 mole \% CO$_3$ as an activator. We used the Czochralski method to grow the crystals. We investigated the scintillation properties of our CsI(CO$_3$) single crystal. The emission spectrum of a CsI(CO$_3$) crystal was measured by using an optical fiber spectrometer. We attached a Photomultipiler tube to the CsI(CO$_3$) crystal; then, we measured the energy resolution, the pulse height spectrum, the linearity and the decay time of the CsI(CO$_3$) single crystal for various gamma-ray energies. We measured the absolute light output by using an avalanche photodiode, and we compared the result with that of a CsI(Tl) single crystal. The $\alpha / \beta$ light ratio and the possibility of pulse-shape discrimination between $\alpha$ and $\gamma$ particle by using an $^{241}$Am alpha source were also studied. 핵물리, 고에너지물리 및 의료영상에서 사용하기 위하여 성능이 우수한 섬광단결정의 개발이 중요하다. 본 연구에서는 CO$_3$ 0.01 mole\%를 첨가한 CsI(CO$_3$) 섬광단결정을 초크라스키방법을 써서 육성하였다. Optics Fiber Spectrometer를 이용하여 육성된 단결정의 발광 스팩트럼을 측정했고, 증폭광센서 (APD)를 사용하여 절대 섬광량을 측정하여 CsI(Tl) 섬광결정과 비교하였다. 광증배관을 부착하여 상온에서 여러 가지 에너지의 감마선에 대한 에너지 분해능, 파고스펙트럼의 선형성 및 감쇄시간을 측정하였다. 그리고, 알파선원을 이용하여 파고스펙트럼을 이용한 알파/감마율 및 감쇄시간의 차이를 이용한 알파/감마 구별에 대한 연구를 수행하였다.

Plateau 및 Rise/Fall 시간의 변화에 따른 안구전정유발근전위의 변화

이여진,한수희,하은정,정용수,곽희붕,박문수,신정은,박홍주 대한평형의학회 2008 Research in Vestibular Science Vol.7 No.2

Background and Objectives: The ocular vestibular evoked myogenic potential (OVEMP) is a recently discovered test of labyrinthine function, analogous to the cervical VEMP. Recent works have demonstrated the existence of OVEMPs, which likely reflect otolith-ocular reflex. The purpose of this study was to identify the optimal plateau and rise/fall times of short tone bursts to detect OVEMPs in healthy subjects. Materials and Methods: Thirteen healthy subjects (26 ears) were included in this study. Surface electromyographic activity was recorded from active electrodes placed inferior to each eye. Stimulation with 500 Hz short tone bursts was used. We used a variety of plateau and rise/fall times. Three different plateau times (1, 2, and 3 ms) and rise/fall times (0.5, 1, and 2 ms) were used. The incidence, amplitudes and latencies were compared. Results: VEMP responses were clearly observed in all 26 ears at the plateau time of 2 ms and two rise/fall times (0.5 and 1 ms). The amplitudes in the individual ears tested were lower at the rise/fall time of 2 ms than at the other conditions. The amplitudes were lower at the plateau time of 3 ms compared to the other conditions. When the rise/fall time was prolonged from 0.5 to 2 ms, the n1 and p1 latencies were prolonged in parallel. However, there was no such change in latencies according to the plateau times. Conclusions: Our findings show that the ideal stimulation pattern for evoking OVEMP is at the rise/fall times of 0.5 or 1 ms and the plateau time of 2 ms. The waveform morphology of the VEMP responses observed with this stimulation pattern was simultaneously the most constant and marked. Background and Objectives: The ocular vestibular evoked myogenic potential (OVEMP) is a recently discovered test of labyrinthine function, analogous to the cervical VEMP. Recent works have demonstrated the existence of OVEMPs, which likely reflect otolith-ocular reflex. The purpose of this study was to identify the optimal plateau and rise/fall times of short tone bursts to detect OVEMPs in healthy subjects. Materials and Methods: Thirteen healthy subjects (26 ears) were included in this study. Surface electromyographic activity was recorded from active electrodes placed inferior to each eye. Stimulation with 500 Hz short tone bursts was used. We used a variety of plateau and rise/fall times. Three different plateau times (1, 2, and 3 ms) and rise/fall times (0.5, 1, and 2 ms) were used. The incidence, amplitudes and latencies were compared. Results: VEMP responses were clearly observed in all 26 ears at the plateau time of 2 ms and two rise/fall times (0.5 and 1 ms). The amplitudes in the individual ears tested were lower at the rise/fall time of 2 ms than at the other conditions. The amplitudes were lower at the plateau time of 3 ms compared to the other conditions. When the rise/fall time was prolonged from 0.5 to 2 ms, the n1 and p1 latencies were prolonged in parallel. However, there was no such change in latencies according to the plateau times. Conclusions: Our findings show that the ideal stimulation pattern for evoking OVEMP is at the rise/fall times of 0.5 or 1 ms and the plateau time of 2 ms. The waveform morphology of the VEMP responses observed with this stimulation pattern was simultaneously the most constant and marked.

신호대잡음비가 인공와우착용자의 GDT에 미치는 영향

한수희,구성민,하은정,신정은,박홍주,임덕환 한국청각언어재활학회 2007 Audiology and Speech Research Vol.3 No.2

음향 신호의 자세한 시간적 변화를 변별하는 능력은 speech discrimination과 sound localization과 같은 청각인 지기능에 중요한 것으로 알려져 있다. 이러한 temporal processing의 기능을 측정하는 방법 중 하나는 자극 음이 중간에 연결되지 않고 끊어지는 순간을(temporal gap)는 감지하는 역치를 찾는 것이다. 이러한 피검자가 인지하는 가 장 작은 크기의 gap이 Gap Detection Threshold(GDT) 로 기록될 수 있다.2) 인공와우 환자들은 speech discrimination에서 흔히 다양한 형태의 어려움을 보이며, 이는 언어 의 구조 중에서 세밀한 시간적 변화를 정확하게 변별하는 능력과 연관되어 있으며, 이러한 변별을 위한 중요한 변수 중 하나로 temporal resolution을 지적할 수 있다. 이 temporal resolution은 GDT 측정을 통해 확인할 수 있으며, 본 연구에서는 인공와우 환자들에게서 소음이 없는 Quiet 조건과 다양한 신호대 잡음비(SNR) 크기를 갖는 동측 배경 소음 조건에서의 within-channel GDT를 측정하여 정상 그룹(Normal Hearing, NH)과 노인성난청(Elderly Hearing Loss, EHL)그룹의 결과와 비교하고자 하였다.

조직배양에 의한 약용식물자원의 생약학적 연구(Ⅸ) : 카르두스 마리아누스의 캘러스 형성에 미치는 아미노산의 영향 Effects of amino acids on the callus formation of Silybum marianum

장승엽,성락선,강신정,이춘길,하광원,류점호,이경순 식품의약품안전청 1998 식품의약품안전청 연보 Vol.2 No.-

카르두스 마리아누스의 조직배양에 있어서 식물생장조절물질에 따른 캘러스의 형성과 캘러스 증식에 미치는 아미노산의 영향을 조사하였다. 그 결과 캘괴스의 유도를 위하써는 2,4-D 0.Img/f에 kiBetin 1.Omg/j 조합처리가가장효과적이었다. 아미노산의 처리에 파른 캘러스의 증꼭상태는 L-phenylalanine>L-leucine)L-valine=L-thrrf nine의 순으로 캘러스 증식이 잘 되었다. 특히 L-phenylalanine은 어느 농도에서도 캘러스 증식이 잘 되었으며 Of mg/스과 농도에서 가장 활발하였다. 아미노산의 처리를 따른 캘러스에서의 silybin 및 silymarin의 성분함량을조각한 결파 L-phenylalanine 0.Smg/f의 농도에서 silybiii의 함량이 0.19mg/g으로써 가장 높게 나타났다. Tissue culture was accomplished to j;nvestigate the effects of antino acids on callus proliferation of fifroun wtarianuuL The callus induction frein the leaf was most effective iu combinationtreatment with 0.Img/f 2.4-D and 1.emg/j kinetin. The callus proliferation was most effective in the me-dium supplemented with 0.Img/f 2,4-D and 0-Smg/f L-phenylalanine. In this condition, the content ofsilybin in callus was 0.19mg/g.

주시 방향에 따른 안구전정유발근전위의 변화 양상

이여진,한수희,하은정,안재윤,박가현,정용수,곽희붕,박문수,신정은,박홍주 대한이비인후과학회 2009 대한이비인후과학회지 두경부외과학 Vol.52 No.3

Background and Objectives:Recent works have demonstrated the existence of ocular vestibular evoked myogenic potentials (OVEMPs), which reflects otolith-ocular reflex. The purpose of this study was to identify an appropriate gaze position to detect OVEMPs produced by air-conducted sound stimulation in healthy subjects. Subjects and Method:Twenty four healthy subjects (35 ears) were included in this study. Surface electromyographic activity was recorded from active electrodes placed inferior to each eye. Stimulation with 500 Hz short tone bursts was used to activate the vestibular end-organs. Results:Sound stimulation evoked negative-positive biphasic responses on both ipsilateral and contralateral eyes, while responses were contralateral eye-dominant. Contralateral eye responses had higher response incidence and larger amplitudes. Altering the direction of gaze generally changed the incidence and size of the inter-peak amplitudes. The higher incidence and larger amplitudes were recorded when the eyes are directed to the superior and ipsilateral side to the sound stimulation. In that gaze position, the amplitude was 5.3 μV, the first negative peak latency was 10.5 ms and the following positive peak latency was 15.4 ms on the average. Conclusion: OVEMPs can be evoked using an air-conducted 500 Hz tone burst and are best recorded contralaterally with a upward gaze towards the source of sound stimulation. Further changes of the test parameters are needed for higher and consistent responses.

정상인에서 진동 및 소리 자극에 의한 경부 전정유발근전위

나보라,한수희,하은정,이여진,박문수,김재명,신정은,박홍주 대한이비인후과학회 2011 대한이비인후과학회지 두경부외과학 Vol.54 No.3

Background and Objective ZThe aim of this study was to investigate and compare the incidence and the characteristics of parameters of cervical vestibular-evoked myogenic potentials (cVEMPs) using air-conducted (AC) and bone-conducted (BC) stimulations in normal subjects. Subjects and Method Twenty-four normal subjects (48 ears) with normal hearing and no previous history of dizziness were included. cVEMP responses were recorded by surface el-ectrodes on sternocleidomastiod muscles in response to AC and BC stimuli sequentially. Variances of parameters, including thresholds, amplitudes and interaural amplitude difference ra-tios (IADR), were analyzed and compared. Results cVEMP responses were clearly observed in all 48 ears in both AC and BC cVE-MP tests. There was no significant difference in latencies (p1 and n1). However, the thresholds in BC cVEMP testing were significantly lower than those in AC cVEMP testing, when compared to the maximum stimulation level. Interaural amplitude difference ratios showed no sig-nificant difference in between the two tests, although amplitudes at maximum stimulation in-tensity in BC cVEMP testing were significantly larger than those in AC cVEMP testing. Conclusion This study shows that BC cVEMP testing shows lower thresholds compared to AC cVEMP testing in normal subjects, suggesting that the threshold value can be used as a parameter in detecting vestibular dysfunction in the clinic. Further studies in patients with vari ous vestibular disorders are needed.

Enhanced Solubility and Bioavailability of Flurbiprofen by Cycloamylose

백형희,So Young Kwon,노신정,Won Seok Lee,Ho-Joon Yang,하정미,김용노,용철순,최한곤 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.3

The effect of cycloamylose on the aqueous solubility of flurbiprofen was investigated. To improve the solubility and bioavailability of flurbiprofen (poor water solubility), a solid dispersion was spray dried with a solution of flurbiprofen and cycloamylose at a weight ratio of 1:1. The physicochemical properties of solid dispersions were investigated using SEM, DSC, and X-ray diffraction. The dissolution and bioavailability in rats were evaluated compared with a commercial product. Cycloamylose increased solubility of flurbiprofen approximately 12-fold and dissolution of it by 2-fold. Flurbiprofen was present in an unchanged crystalline state, and cycloamylose was a solubilizing agent for flurbiprofen in this solid dispersion. Furthermore,the dispersion gave higher AUC and Cmax values compared with the commercial product, indicating that it improved the oral bioavailability of flurbiprofen in rats. Thus, the solid dispersion may be useful to deliver flurbiprofen with enhanced bioavailability without changes in crystalline structure.