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성숙도가 다른 인삼 접합자배의 (接合子胚) 자엽으로부터 형성되는 체세포배의 (體細胞胚) 기원
최용의(Yong Eui Choi),소웅영(Woong Young Soh) 한국식물학회 1994 Journal of Plant Biology Vol.37 No.3
Excised cotyledon segments of ginseng zygotic embryos at various developmental stages were cultured on MS basal medium from which somatic embryos were directly induced. The frequency of somatic embryo formation on segments declined with the advancing zygotic embryo maturity. All of the cells in the cotyledons of immature zygotic embryos were smaller and more densely cytoplasmic than those in mature embryos. Histological examinations revealed that the poly-somatic embryos formed on immature embryos were of multi-cell origin and derived from the epidermal and subepidermal cell layers. However, in the cotyledon of germinating zygotic embryos, only the epidermal cells were densely cytoplasmic and singularly competent to develop into somatic embryos resulting into single embryos at a frequency of 100%.
오가피(Acanthopanax sessiliflorus)의 체세포배로부터 식물체 재생에 미치는 GAa<sub>3</sub>와 Charcoal의 영향
이강섭,최용의,심옥경,주선아,신정순,정재훈,김영신,김이엽,Lee, Kang-Seop,Choi, Yong-Eui,Sim, Ock-Kyeong,Joo, Sun-Ah,Shin, Jeong-Sun,Jeong, Jae-Hun,Kim, Young-Shin,Kim, Ee-Yup 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.4
To establish the optimum condition for plant regeneration from somatic embryos of Acanthopanax sessiliflorus Rupr. et Maxim, a medicinal plant, somatic embryos were induced from zygotic embryo-derived embryogenic callus in hormoen-free MS medium. To induce plantlet conversion, cotyledonary somatic embryos were cultured on MS solid medium with GA$_3$at various concentrations (0~10 mg/L) for three weeks. Plantlets were transferred to 1/3 MS solid medium with 0.5% charcoal for 7 weeks. Stem length was increased proportionally to the concentration and treatment period of GA$_3$. Also, the highest leaf width (8.9 mm) and leaf number (2.84) of plantlet were obtained when plantlets were converted on 5,10 mg/L GA$_3$pretreatments, respectively. The highest plant conversion frequency (66.7%) was obtained when the somatic embryos were cultured on medium containing 5 mg/L GA$_3$ for 3 weeks and then were transferred to 1/3 MS medium with 0.5% charcoal. The highest survival rate of soil transfer was 90% when plantlets were regenerated on medium with 5 mg/L GA$_3$ for 3 weeks and then transferred to plastic pots containing vermiculite and sand mixture for 4 weeks.
구기자나무 (Lycium chinense)의 모상근 유도 및 생물반응기 배양
배기화,김윤수,정재훈,김영선,최용의,윤의수,Bae, Ki-Hwa,Kim, Yun-Soo,Jeong, Jae-Hun,Kim, Young-Seon,Choi, Yong-Eui,Yoon, Eui-Soo 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.4
구기자나무의 잎 절편을 A. rhizogenes strain, 15834와 공동 배양하여 유도된 모상근의 최적배양조건을 결정하기 위하여 MS배지와 sucrose의 농도를 다양하게 처리한 결과, 1/2 MS 배지에서 생체중 (12.3 g)과 건물중 (1.4 g)으로 가장 높게 나타났으며, 탄소원으로 사용된 sucrose에서는 3% 농도에서 생체중과 건물중이 가장 양호하게 나타났다. 이와 같이 결정된 MS농도와 sucrose의 농도를 이용하여 대량배양을 하고자 10 L의 air lift bioreactor에서 대량 배양한 결과 역시 1/2 MS와 3% sucrose의 농도를 사용한 처리구에서 가장 높은 생체중과 건물중의 증가를 보였다. 하지만, 1/2 MS에 5% sucrose를 처리한 bioreactor배양에서도 3% sucrose처리에서와 마찬가지로 생체중과 건물중이 높게 나타났다. This article was conducted to induce the transgenic hairy roots and determine the effect of culture conditions on optimum growth of hairy roots by Agrobacterium rhizogenes strain, 15834 in Lycium chinense Miller. Hairy roots of L. chinense Miller. were induced from leaf segments by co-cultivation with A. rhizogenes. When the hairy roots were cultured in various MS medium strength and sucrose concentrations, the highest growth of hairy roots was observed in half-strength MS media supplemented with 3% sucrose, respectively. In air lift bioreactor cultures, the liquid medium contained with 1/2 MS and 3% sucrose was also the best for optimum growth of hairy roots.
가시오갈피 접합자배로부터 체세포배 발생 과정의 구조적 특성
최용의,소웅영 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-
가시오갈피 접합자배를 1.0 mg/L 2,4-D가 첨가된 MS 배지에 배양하였을 때, 배양재료 표면에서 직접 체세포배가 발생되었다. 접합자배를 온전한 상태로 배양하였을 때에 비하여 상처를 주어 배양하였을 때 배발생률이 증가되었으며, 부위별로 절단하여 배양하였을 경우 하배축에서 발생률이 높았다. 체세포배발생에 대한 조직학적 관찰 결과, 유근부위가 잘록한 단일배와 여러개의 배가 서로 융합된 다배가 관찰되었다. 이들 두가지 모양의 체세포배는 접합자배의 부위마다 발생빈도가 달랐는데, 자엽이나 하백축하부에서는 단일배가 주로 관찰되었고, 배형성능이 높은 부위인 자엽절이나 하배축상부에서는 다배의 발생률이 높았다. 단일배는 표피의 단세포 기원되었으나 다배는 모두 표피 및 하표피의 다수세포에서 기원되었음이 조직학적 관찰로 확인되었다. When the zygotic embryos of Acanthopanax senticosus were cultured on MS medium containing 1.0 mg/L 2,4-D, somatic embryos were produced directly from the surface without forming an intervening callus. The frequency of embryo formation was higher in the wounded zygotic embryos than in the intact ones. Hypocotyl explants showed a higher frequency of somatic embryo formation that cotyledon or radicle explants. Two typeds of somatic embryos were observed : one was single embryos with closed radicle which were mainly formed on cotyledon or radicle part; and the other was polyembryos of which hypocotyls were fused one another showing multicotyledons with a single hypocotyl. Histological observation revealed that single embryos originated from the single epidermal cells while polyembryos were derived from massive cells of epidermis of subepidermis.
참당귀(Angelica fifas Nakai)의 캘러스로부터 체세포배 발생에 미치는 계대배양 주기의 영향
최용의,소웅영 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-
Embryogenic callus was produced form leaf segments cultured on medium containing 1.0 mg/L 2,4-D. The callus gave rise to somatic embryos after transfer the embryogenic callus to medium without 2,4-D. Also, somatic embryogenesis occured from aged callus on medium containing 2,4-D. To investigate the effects of culture periods on somatic embryogenesis the embryogenic calli were cultured for 2, 4, 6 and 8 weeks on medium containing 1.0 mg/l 2,4-D and then transferred to medium with or without 2,4-D. In prolonged preincubation, somatic embryos were formed early and at high frequency and their development normally occured. When subcultured on medium containing 2,4-D, the callus preincubated for 6 to 8 weeks was capable of producing somatic embryos. Histological observation revealed that 4 weeks-old embryogenic callus on medium containing 2,4-D was composed of mainly typical embryogenic cells, whereas the 8-weeks-old callus was composed of nonembryogeinc cells and that. In the developing state of somatic embryo on medium without 2,4-D, all the cells of callus were in vacuolated and elongated except developing somatic embryos.