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This study was carried out to develop the improved useful mutants for yield orcomposition of stevia plants using the gamma ray or chemical mutagens treatments. The seeds ofstevia ‘Suwon No. 11’ were irradiated up to 400 Gy of gamma ray. Chemical mutagens weretreated on the seeds of the ‘Suwon No. 11’ using 0.07% colchicine, 10 mM sodium azide, or 10mM NMU for various durations. The germination rate, and shoot and root growth of seedlingwere estimated at 30 days after gamma ray irradiation or chemical mutagen treatment, and theplant height, the number of branches, and leaf length and width were examined at 3 months aftermutagenesis treatments. In the case of gamma ray treatments, the germination rate and earlystagegrowth were decreased as the increase of radiation dose, and the 50% lethal dose was foundto be 200 Gy. the plant height was decreased as the increase of radiation dose, while the numberof branches per plant and leaf length were increased. Leaf shape was modified to the relativelylonger one compared to the control, which was identified more apparently at the treatments ofhigher than 150 Gy. In the treatment of chemical mutagens, the rate of germination and survivalwere decreased as the increase of incubation time. The 50% lethal dose for germination rate wereidentified as the conditions of the 15 hours incubation in 0.07% colchicine, the 4 hrs in 10 mMsodium azide, and the 2 hrs in 10 mM NMU, in the three chemical mutagens treatments. Chemicalmutagens had no influence on shoot growth, while root growth was increased, especially as theincubation time was extended. The highest root growth occurred in the NMU treatment at 6 hrsincubation time. The plant height was decreased as the increase of incubation time in the chemicalmutagens treatments. Among the chemical mutagens, NMU was the most effective to induce themutants with long-shaped or the least lobed leaves.
당근의 배양세포로부터 체세포배의 발생과정에 미치는 아스콜빈산 및 dehydroascorbic acid의 영향을 밝히기 위하여 본 실험을 시도하였다. 비배발생세포의 배양에 처리된 아스콜빈산은 세포증식을 촉진시켰을 뿐인데 dehydroascorbic acid는 세포증식을 억제시키면서 배발생세포로 전환시킨 효과가 있었다. 배발생세포의 배양에 처리된 아스콜빈산은 체세포배 발생을 억제시켰지만 dehydroascorbic acid는 체세포배발생을 촉진시켰다. 그러나 이와 같은 발생촉진은 구상배에서 중단되므로 성숙에는 오히려 저해적이었다. 이상의 결과로부터 당근의 캘러스배양에 dehydroascorbic acid를 처리하여 빠른 시일내에 배발생캘러스를 확보한 다음 dehydroascorbic acid 첨가 배발생 배지에서 초기배발생기간 배양 후 MS 기본배지로 옮겨 배양하면 고빈도의 체세포배생산 실험계가 확립될 것으로 판단된다. This study was conducted to elucidate the effects of ascorbic acid and dehydroascorbic acid on somatic embryogenesis from the cultured cells of carrot. Ascorbic acid in culture medium merely stimulated the proliferation of non-embryogenic cells but dehydroascorbic acid in medium induced embryogenic cells from non-embryogenic cells accompanying the inhibition of cell proliferation. Ascorbic acid in medium inhibited somatic embryogenesis from embryogenic cells while dehydroascorbic acid in medium enhanced somatic embryogenesis from the cells as well as non-embryogenic cells. This enhancement was limited to globular embryos and the maturation to cotyledonary embryos was inhibited by dehydroascorbic acid treatment. From the above results it is suggested that carrot callus cultures on medium containing dehydroascorbic acid could quickly induce embryogenic cells. In addition after brief culture of embryogenic cells on development medium containing dehydroascorbic there by acid the subculture of the cells to MS basal medium resulted in the high frequency production of somatic embryos.
- This study was carried out to establish the micropropagation system of Bupleurum latissimumNakai that is aKorean native endangered species. Callus were induced from the leaf, petiole and floral bud and the percentage of callusformation was highest in the floral bud on the MS medium containing 2.0 mg· L-1 2,4-D. Especially, callus inducedfrom floral bud was formed 77.8% and the percentage of shoot formation was 42.6% on the MS medium containing 2.0mg· L-12,4-D plus 1.0 mg· L-1TDZ. For simultaneously callus formation and shoot regeneration, 1/2 MS medium wasmore effective than MS medium. The percentage callus formation, shoot regeneration and rooting were 46.3%, 13.0%,13.0% in 1/2 MS medium, respectively. Soot regeneration from callus was good in 1/2 MS medium supplemented with2.0 mg· L-12,4-D plus 1.0 mg· L-1BA where percentage of shoot regeneration was 74.1%, and the number of shoot perexplant was 2.4. The percentage of rooting was lowest (57.8%) in control while it was highest (97.8%) in 1.5 mg· L-1NAA. In acclimatization of regenerated plantlets, the percentage of survived plantlets was highest (86.1%), and plantheight, root length and fresh weight were good in the soil for horticulture.Key words - Bupleurum latissimum, Callus, Shoot, Regeneration, Propagation韓資植誌 Korean J. Plant Res. 20(4):367∼374(2007)한 거의 이루어진 적이 없다.따라서 본 연구는 멸종 위기에 처한 섬시호의 대량 증식을 위해 조직배양을 통한 번식체계를 확립하고자 적합한 배양 절편체를 선정하고 선정된 절편체로부터 캘러스 유도, 식물체 재분화,발근에 효과적인 식물생장조절물질의 종류와 농도를 구명하며재분화 식물체의 생존율을 향상시킬 수 있는 순화용토를 선발하고자 하였다.재료 및 방법식물재료본 실험에서 사용한 섬시호는 종자를 2005년 8월 환경부 서식지외 보전기관인 기청산식물원에서 채취하여 전북대학교 온실에서 발아시킨 후 실험재료로 사용하였다.치상 절편체 부위 및 생장조절물질에 따른 캘러스 유도캘러스 형성률이 높은 치상 절편체를 찾기 위하여 식물체가약 30cm로 자랐을 때 5∼7번째의 잎과 엽병을 채취하여 잎은 5
This study was carried out to induce plant regeneration via multiple shoot formation from sucker explants ofRubus fruticosusL. × R. parvifoliusL. To induce adventitious shoots, the sucker explants were sterilized in 1.2%sodium hypochlorite (NaOCl) solution, and then were cultured on the full and 1/2 MS solid medium supplemented withBA (0.1, 0.5, 1.0, 2.0㎎L-1explants was obtained from the full MS medium with 1.0㎎L-1 BA. The highest shoot number (3.7) per explant wasobtained from the full MS medium with 0.5㎎L-1 BA. After 12 weeks of culture, the number of shoots (15.4) perexplant was increased. The highest frequency (95%) of root formation was obtained from the 1/2 MS medium, when theexplant with shoot were cultured on the full, 1/2 and 1/4 MS medium. The survival rate of the plantlets after transfer toprocedure can be applied for an efficient mass propagation of Rubus fruticosusL. × R. parvifoliusL.
This study was carried out to obtain the basic informations on the characteristics ofgametophytes formation and embryo developement in Dicentra spectabilis. Microsporemother cells developed from archesporial cells, start meiosis when flower bud length reachesaround 1 mm, formed tetrahedral type tetrad. The 4 microspores were separated. They weredeveloped to male gametophytes, respectively. Megaspore mother cells were observed whenflower bud length was 4~5 mm. The developemental type of megaspore was polygonum andembryo sac was amphitropous. Three large and distinctive antipodals did not degeneratedand remained after embryo sac was developed. When the male and female gametophyteswas fully developed, the length of stamen and style was very similar or stamen was shorterabout 0.5 mm than that of style. This result indicates that self-fertilization can be occurredin this species. After fertilization, developing zygotic embryos showed various stages ofdevelopment from globular to cotyledonary embryos, and zygotic embryo in seed scatteringtime seemed to have an early cotyledonary stage.