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급성기 중풍환자에서 비만 및 혈액지표의 기허 및 화열 변증의 차이에 대한 고찰
차민호,김소연,임지혜,강병갑,고미미,김노수,이정섭,방옥선,Cha, Min-Ho,Kim, So-Yeon,Lim, Ji-Hye,Kang, Byung-Kab,Koo, Mi-Mi,Kim, No-Soo,Lee, Jeong-Sub,Bang, Ok-Sun 대한한방내과학회 2009 大韓韓方內科學會誌 Vol.30 No.4
Object : In the present study, we investigated the obesity and blood parameters between Qi-deficiency and Fire/Heat pattern identification/syndrome differentiation (PI/SD) in acute stroke patients. Materials and Methods : A total of 391 stroke patients within 7 days after onset were consecutively recruited from 12 hospitals across South Korea from Nov. 1st, 2006 to Jun. 31st, 2009. They were diagnosed as Fire/Heat or Qi-deficiency among five PI/SD subtypes by two independent stroke experts. We investigated the differences of obesity and blood characteristics between Fire/Heat and Qi-deficiency by statistical analyses. Results : In male subjects, obesity was significantly associated with Fire/Heat PI/SD. The averaged mean BMI ($24.13kg/m^2$) and waist circumference(89.34cm) of the Fire/Heat group were higher than those of the Qi-deficiency group ($22.60kg/m^2$ and 83.43 cm, respectively). The number of obese patients was larger in the Fire/Heat group than in the Qi-deficiency group (p = 0.001). Hyperlipidemia was also related with Fire/Heat. However, obesity was not associated with PI/SD in female subjects where the number of hyperlipidemic patients was higher in the Qi-deficiency group. Among blood parameters, the levels of triglycerides and fasting blood sugar were higher in the Fire/Heat group compared with the Qi-deficiency group in male subjects. However, total cholesterol of the Qi-deficiency group was higher than in the Fire/Heat group among female subjects. Conclusion : This study shows that obesity and hyperlipidemia are significantly difference between Qi-deficiency and Fire/Heat. We suggests that PI/SD may be associated with clinical characteristics and large population study between PI/SD and clinical characteristics including blood parameters are needed.
K-562 백혈병 세포주에서 저근백피와 Gleevec을 처리에 의한 유전자 발현 비교 분석
차민호,안원근,전병훈,윤용갑,윤유식,Cha, Min-Ho,An, Won-Gun,Jeon, Byung-Hun,Yun, Yong-Gab,Yoon, Yoo-Sik 대한동의생리학회 2005 동의생리병리학회지 Vol.19 No.3
In this study, we investigated gene expression patterns induced by Ailanthus altissima extract and compared it with Gleevec, a well-known anti-leukemia drug, in K562 chromic leukemia cells. Ailanthus altissima extract(100 ug/ml) and Gleevec(50 ug/ml) were treated to cells for 1h, 2h, 4h, and 16h and total RNA was extracted. Gene expressions were evaluated using cDMA microarray, in which 24,000 genes were spotted. Hierarchical clustering analysis showed that expression of genes included in two clusters were increased or decreased time dependently by both Ailanthus altissima extract and Gleevec. Genes included in another cluster were induced by Ailanthus altissima extract but not by Gleevec. In biological process analysis, expression of genes involved in apoptosis, growth arrest and DNA-damage were increased, but genes stimulating cell cycle were decreased. This study provides comprehensive comparison of the patterns of gene expression changes induced by Ailanthus altissima extract and Gleevec in K-562 leukemia cells.
고지방식이를 섭취한 mouse의 hypothalamus에서의 유전자군의 발현양상 변화
차민호,김경선,강봉주,심웅섭,윤유식,Cha, Min-Ho,Kim, Kyung-Seon,Kang, Bong-Joo,Sim, Woong-Seop,Yoon, Yoo-Sik 한국한의학연구원 2003 한국한의학연구원논문집 Vol.9 No.1
Obesity is caused by imbalance of energy intake and expense. If energy intake is more than its expenditure, body does fat accumulation and affects body weight. It can be fetal disease although obesity is not disease in itself. Central regulatary system is affected by many neurotransmitters regulating .food intake in brain. Hypothalamus was known as one of food intake regulation in CNS. In order to investigate gene expression difference in hypothalamus by different nutrient, we used C57/BL6 control mouse and db-/db- mouse. They divided each of two group with mouse, and fed control diet and high-fat diet for 4 weeks. Each of control and high-fat diet contained 11.7% and 59.7% fat, respectively. Then we performed microarray assay with them. We compared among changed genes in hypothalamus region. In the results, we observed that increased genes were more than decreased genes. Although hypothalamus size of db-/db- mouse is smaller than that of C57/BL6, more genes were affected in db-/db- mouse. In this study, many genes are affected by nutrient in hypothalamus region.
Streptomyces Peucetius에서의 ${\varepsilon}$-rhodomycinone 추출 및 이종균주에서의 rhodomycin D 생산 연구
박성희,차민호,김은정,윤여준,송재경,이희찬,류광경,김병기,Park, Sung-Hee,Cha, Min-Ho,Kim, Eun-Jung,Yoon, Yeo-Joon,Sohng, Jae-Kyung,Lee, Hee-Chan,Liou, Kwang-Kyoung,Kim, Byung-Gee 한국생물공학회 2008 KSBB Journal Vol.23 No.1
Streptomyces peucetius가 생산하는 anthracycline 계열의 doxorubicin은 치료목적으로 사용되는 중요한 항암제 중 하나이다. Doxorubicin은 rhodomycin D에서부터 몇 단계의 생합성 과정을 더 거쳐 생산되는데, 생물학적 활성을 갖기 위해서는 deoxy-sugar의 전이가 반드시 일어나야 한다. 본 논문에서는 이종균주인 Streptomyces venezuelae에 11개의 유전자를 형질 전환하여 TDP-L-daunosamine를 생산하고 이것을 ${\varepsilon}$-rhodomycinone에 전이하여 rhodomycin D를 생산하는 연구를 수행하였다. S. peucetius 유래의 7개 유전자 dnmU, T, J, V, Z, Q, S.를 당 합성 및 전이를 위해 plasmid 형태로 전이하였으며, S. venezuelae의 desIII, IV와 doxorubicin 내성 유전자인 drrA, B는 chromosomal DNA에 삽입하였다. Aglycone 기질인 ${\varepsilon}$-rhodomycinone을 확보하기 위하여 6L의 고체 배지에 S. peucetius를 배양하여 유기용매로 추출하고 preparative HPLC로 분리 정제하였다. 결과적으로 이종균주인 S. venezuelae에서 ${\varepsilon}$-rhodomycinone에 당 전이가 일어난 생산물을 확인함으로써 deoxy-sugar의 생합성 및 전이에 필요한 최소한의 유전적 정보를 확인할 수 있었다. 또한, 유사서열 단백질 모델링을 통하여, 최초로 당 전이 반응에 필수적인 도움효소 DnrQ의 구조를 예측하였다. Anthracycline antibiotics doxorubicin (DXR) is clinically important cancer therapeutic agent produced by Streptomyces peucetius. DXR result by further metabolism of rhodomycin D (RHOD) and require a deoxy-sugar component for their biological activity. In this study, production of TDP-L-daunosamine and its attachment to ${\varepsilon}$-rhodomycinone (RHO) to generate RHOD has been achieved by bioconversion in Streptomyces venezuelae that bears eleven genes. S. peucetius seven genes (dnmUTJVZQS) were transformed by plasmid and S. venezuelae two genes desIII, IV and two more S. peucetius drrA, B genes were integrated into chromosomal DNA. To generate the feeding substrate RHO, 6L S. peucetius grown on agar plate was harvested, extracted with organic solvent and then purified using preparative HPLC. Recombinant S. venezuelae grown on agar plate containing RHO was harvested and its n-butanol soluble components were extracted. The glycosylated product of aromatic polyketide RHO using heterologous host S. venezuelae presents the minimal information for TDP-L-daunosamine biosynthesis and its attachment onto aglycone. Moreover, the structure of auxiliary protein, DnrQ, was predicted by fold recognition and homology modeling in this study. This is a general approach to further expand of new glycosides of antitumor anthracycline antibiotics.
황금(黃芩, Scutellariae Radix)의 지방축적억제 효능연구
김경선,차민호,이수원,윤유식,Kim, Kyeong-Seon,Cha, Min-Ho,Lee, Soo-Won,Yoon, Yoo-Sik 한국한의학연구원 2003 한국한의학연구원논문집 Vol.9 No.2
Obesity is caused by unbalance of energy intake and expenditure, which results in extra accumulation of adipose tissue. Obesity is directly related to metabolic diseases such as diabetes, hyperlipidemia, fatty liver and so on. To investigate the anti-obesity effects of Scutellariae Radix, 70% EtOH extract and water extract of it were tested by in vitro and in vivo studies of fat accumulation. 3T3-L1 preadipocyte cell line was used in a in vitro study of fat accumulation. After 3T3-L1 cells were induced to differentiate into adipocytes, S. radix extract were added and fat accumulation was measured by oil red O staining. In vivo study showed that weight and epididymal/ retro-peritoneal adipose tissues were significantly reduced in mice fed Scutellariae Radix extract compared with control group. Especially, mice fed Scutellariae Radix extract showed reduced serum triglyceride and glucose levels. When adipose tissues were analyzed by microscope, mean adipocyte size was significantly reduced in Scutellariae Radix extract-fed mice. Therefore, this study showed inhibitory effects of Scutellariae Radix on in vitro and in vivo fat accumulation.
네트워크 분석기반을 통한 대마 줄기 및 뿌리 추출물의 약리효능 예측연구
신명자(Myung-Ja Shin),차민호(Min-Ho Cha) 한국생명과학회 2024 생명과학회지 Vol.34 No.3
Canabas sativa L. (marijuana and hemp)는 전 세계적으로 널리 재배되는 식물로 식품, 의약품 등의 재료로 사용되었다. 본 연구는 네트워크 약리학을 이용하여 대마 줄기 및 뿌리 추출물의 기능적 효과를 예측하고 이들의 새로운 기능을 알아보고자 하였다. 줄기 및 뿌리 에탄올 추출물의 성분은 GC/MS로 확인하였고, 성분과 단백질 간의 네트워크는 STIHICI 데이터베이스를 이용하여 알아보았다. 성분과 연결된 단백질의 작용기전은 KEGG pathway 분석을 수행하였다. 추출물의 효과는 실시간 PCR을 이용하여 lysophosphatylcholine 유도 THP-1 세포에서 확인하였다. 줄기 및 뿌리 추출물에서 각각 21개 및 32개의 성분이 확인되었다. 줄기 및 뿌리의 성분과 연결된 단백질은 각각 147개, 184개의 단백질이었다. KEGG pathway 분석결과 MAPK signaling pathway를 포함한 69개의 경로가 추출물에 의해 공통적으로 영향을 받는 것으로나타났다. 경로 네크워크를 이용한 추가 조사 결과, Terpenoid backbone biosynthesis 추출물 및 MVK와 MVD 의해 영향을 받을 가능성이 높으며, 유전자 발현은 추출물에 의해 LPC 유도 THP-1 세포에서 감소하였다. 따라서 본 연구에서는 대마 줄기 및 뿌리 에탄올 추출물이 다양한 경로로 영향을 미칠 수 있음을보여주었고, 이러한 결과는 대마의 효과를 예측하고 연구하기 위한 기초 정보를 제공할 것으로 사료된다. Cannabis sativa is a plant widely cultivated worldwide and has been used as a material for food, medicine, building materials and cosmetics. In this study, we assessed the functional effects of C. sativa stem and root extracts using network pharmacology and confirmed their novel functions. The components in stem and root ethanol extracts were identified by gas chromatography-mass spectrometry analysis, and networks between the components and proteins were constructed using the STICHI database. Functional annotation of the proteins was performed using the KEGG pathway. The effects of the extracts were confirmed in lysophosphatidylcholine-induced THP-1 cells using real-time PCR. A total of 21 and 32 components were identified in stem and root extracts, respectively, and 147 and 184 proteins were linked to stem and root components, respectively. KEGG pathway analysis showed that 69 pathways, including the MAPK signaling pathway, were commonly affected by the extracts. Further investigation using pathway networks revealed that terpenoid backbone biosynthesis was likely affected by the extracts, and the expression of the MVK and MVD genes, key proteins in terpenoid backbone biosynthesis, was decreased in LPC-induced THP-1 cells. Therefore, this study determined the diverse function of C. sativa extracts, providing information for predicting and researching the effects of C. sativa.