Association of serum resistin with blood stasis syndrome in traditional Korean medicine for metabolic diseases: A cross-sectional multicenter observational study

고미미,Min-Ho Cha,정지연 한국한의학연구원 2021 Integrative Medicine Research Vol.10 No.3

Background: Blood stasis syndrome (BSS) is considered as the cause of several chronic disease including metabolic diseases in traditional East Asian medicine. In this study, we investigated the levels of serum resistin and other proteins related to metabolic syndrome (MS) and several other diseases categories to identify the association with BSS. Methods: This was a cross-sectional, multicenter study of patients recruited from seven traditional Korean Medicine (TKM) hospitals. To identify whether there was an association with BSS in specific disease conditions, including MS, serum protein levels were evaluated using the multiplex method. Results: A total of 885 patients (419 patients with BSS, 376 patients without BSS, and 90 healthy controls) participated in the study, and 139 patients had MS. The resistin and insulin levels were significantly higher in patients with BSS than in patients without BSS and normal subjects (P = 0.002 and P = 0.046, respectively). Patients with BSS who had MS exhibited significantly higher resistin levels than those in patients without BSS and normal subjects (P = 0.049). In addition, the levels of serum resistin were significantly correlated with symptoms of the BSS, especially dark red gums, dark facial complexion, and nocturnal pain. Conclusions: Despite several limitations, these results demonstrated that resistin levels are potentially associated with the pathogenesis of BSS in MS. Trial registration number: Clinical Research Information Service (CRIS): KCT0000916.

수량화 II류 분석을 이용한 중풍 변증진단에 관한 연구

고미미,이정섭,강병갑,오달석,방옥선 한국자료분석학회 2010 Journal of the Korean Data Analysis Society Vol.12 No.4

In oriental medicine, pattern identification diagnosis is very important for treatment of the disease. But pattern identification diagnosis has a lack of objectivity because it depends on doctor's subjective point of view and patient's experience. Recently, for oriental medicine's objectivity the importance of quantification in their study has been increased. In this paper, the quantification method II was conducted to the data using the questionnaire item of case report form about stroke diagnosis from 13 oriental hospital, which consisted of 835 patients. Through this study was to investigate the relation between pattern identification type and its parameter. This study had the implication that it established the objective diagnosis model quantitatively on basis of the information which were gathered from qualitative oriental medical diagnostic process, particularly of the pattern identification and its parameters. 한의학에서 질병의 본질을 명확하게 하여 치료하기 위한 방편으로 변증을 중요시하지만 변증은 환자의 주관적인 표현과 의사의 주관적 혹은 유도된 오감을 통하기 때문에 객관적인 진찰이 어렵고 수치화된 자료의 축적이 쉽지 않다. 최근 한의학분야에서도 객관성 확보를 위한 학문적 경험의 수량화연구가 일반화되고 있다. 본 논문에서는 전국 13개 한방병원을 통해 구축된 중풍 환자에 대한 임상자료의 중풍 변증을 중심으로 증례기록지를 토대로 수집된 총 835명의 환자의 데이터를 이용하여 수량화 Ⅱ류 분석을 실시하였다. 이를 통해 전문가에 의해 진단된 변증과 지표와의 연관성을 종합적으로 파악하고자 하였다. 본 연구는 중풍 임상자료들에 대해 질적 자료에 기초하여 내리는 한의진단과정에서 얻어지는 지식, 특히 여기서는 한의학의 고유 진단체계인 변증과 그 지표들을 통계적인 방법으로 객관적으로 수량화하여 모형구축을 시도한 것에 그 의미가 있다고 할 수 있겠다. 또한 향후 한의중풍변증진단 표준안의 수정과정에서 판별에 중요한 지표의 선택이나 개발에 많은 도움이 될 것으로 기대하는 바이다.

최근 3년간 발표된 동의생리병리학회지 논문의 방법론 및 통계처리기법에 관한 보고

고미미(Mi Mi Ko),강병갑(Byeong-Kab Kang),강경원(Kyung Won Kang),박세욱(Sae Wook Park),김보영(Bo Young Kim),김정철(Jung Chul Kim),최선미(Sun Mi Choi) 한의병리학회 2008 동의생리병리학회지 Vol.22 No.1

LIPG promoter polymorphism is associated with ischemic stroke in Korean population

김노수,고미미,차민호,방옥선 한국유전학회 2012 Genes & Genomics Vol.34 No.2

Endothelial lipase (LIPG) is a member of the triglyceride lipase family which includes hepatic lipase and lipoprotein lipase. Its activity is related to clinically important parameters like blood lipid levels, hypertension, and obesity. In this work,we investigated the association of a LIPG promoter polymorphism,rs9958947C>T, with susceptibility to ischemic stroke in a Korean population. A total of 1,144 subjects (656cerebral infarction patients and 488 controls) were enrolled on a voluntary basis. The rs9958947C>T polymorphism was genotyped using the single-base extension method. The association of rs9958947C>T with disease status was evaluated by statistical analyses. The frequencies of the rs9958947 C and T alleles were significantly different between the stroke patient group and control group (OR [95% CI], 1.300 [1.000-1.691],P=0.0449). A significantly higher frequency of the CT+TT genotype was observed in the patient group compared to the control group (CC/CT+TT, OR [95% CI], 1.632 [1.094-2.435],P=0.0164). The results suggest that the T allele of the LIPG promoter polymorphism rs9958947C>T should be considered as a genetic risk factor for ischemic stroke. Further association studies in other ethnic populations would help to generalize this hypothesis.

한국인에서 NPY 유전자의 C-399T 프로모터지역 단일염기다형성과 뇌졸중 유발에서의 연령 및 성 요인이 미치는 영향

김노수,고미미,차민호,방옥선 한국지질동맥경화학회(구 한국지질학회) 2009 한국지질동맥경화학회 추계학술발표논문집 Vol.2009 No.-

어혈 진단 설문지 II의 신뢰도 및 타당도

강병갑,고미미,정지연,이주아,Kang, Byoung-Kab,Ko, Mi Mi,Jung, Jeeyoun,Lee, Ju Ah 대한예방한의학회 2017 대한예방한의학회지 Vol.21 No.1

Objectives : The aims of the current study were to assess the reliability and validity of the CoRe-Ditec-BS Questionnaire-II (BSQ-II; Blood stasis questionnaire-II) with 8 items including 5 items related women added to BSQ-I that was consisted of 36 items and developed in 2013. Methods : Between May 2014 and November 2014, 411 patients from 3 traditional Korean medical hospitals were asked to complete the BSQ-II. Each patient was independently diagnosed with BSS by two traditional Korean medical physicians from the same site. We estimated the internal consistency using Cronbach's ${\alpha}$ coefficient, the discriminant validity using the means score of BSS, and the predictive validity using logistic regression (sensitivity and specificity). Results : The BSQ-II had satisfactory internal consistency (Cronbach's ${\alpha}$ coefficient=0.765) and validity, with significant differences in the mean scores between the BSS($63.60{\pm}9.56$) and non-BSS groups($48.36{\pm}5.93$). The area under the receiver operating curve was about 98%, and the sensitivity and specificity were 91.4% and 94.9%, respectively. Conclusions : These results suggest that the CoRE-Ditec-BSQ-II is more reliable and valid instrument for estimating BSS than BSQ-I.

Human Paraoxonase 1(PON1)의 유전자 다형성에 따른 중풍환자의 습담 변증과의 상관성 연구

임지혜,고미미,이정섭,방옥선,차민호,Lim, Ji-Hye,Ko, Mi-Mi,Lee, Jung-Sup,Bang, Ok-Sun,Cha, Min-Ho 대한한방내과학회 2010 大韓韓方內科學會誌 Vol.31 No.4

Objective : In the present study, we investigated genetic distribution of eight single nucleotide polymorphisms of PON1 between Dampness and Phlegm and non Dampness and Phlegm pattern identification(PI) among Korean stroke patients. Materials and Methods : One hundred forty stroke subject without Dampness and Phlegm and fifty eight stroke subjects with Dampness and Phlegm were participated in this study. After informed consents, eight single nucleotide polymorphisms(SNPs) in PON1 of each subjects were identified by DNA sequencing and primer extension method and statistical analysis was performed to determine the significant difference between Dampness and Phlegm and non Dampness and Phlegm groups. Results : Among anthropometric characteristics and blood parameters, waist circumference and total cholesterol were significantly higher in Dampness and Phlegm. Among 8 SNPs of PON1, frequency of M allele and subjects with M allele in L55M SNP were significantly higher in Dampness and Phlegm group (p=0.0032 and p=0.0053, respectively) but subjects with T allele in C-2033T SNP were lower in Dampness and Phlegm group(p=0.0302). Effect of L55M and C-2033T on Dampness and Phlegm were 3.07% and 1.75%, respectively. Conclusion : Our results suggest that L55M SNP in exon and C-2033T in promoter region of PON1 maybe affect to Dampness and Phlegm pattern identification. However, further study should be carried out to find out the detailed mechanism how L55M and C-2033T can affect Dampness and Phlegm stroke patients.

통계적 뇌파분석(SEEG)

박석윤,고미미 한국자료분석학회 2008 Journal of the Korean Data Analysis Society Vol.10 No.3

Digital electroencephalogram(dEEG) of Qualitative method about electroencephalogram (EEG) analysis can be a useful method to a clinician in brain-wave field that has great ability like discrimination power and accuracy. But to reach the level of professional experience we need to a lot of time and effort with a lot of experience, and we may be a biased by a subjective point of view even though we are specialists. Recently, quantitative electroencephalogram(qEEG) like TM(topographic mapping) are developed and introduced that would replace digital electroencephalogram(dEEG) that which has technical limits. Our work focuses on the research for good EEG signal representations and on careful analysis classification results using statistical EEG analysis(SEEG) comparing digital electroencephalo- gram(dEEG) with quantitative electroencephalogram(qEEG). Statistical electroencephalogram (SEEG) put advantage of two methods together. 뇌파(electroencephalogram: EEG)에 관한 접근법 가운데 정성적 분석은 시각적 뇌파분석(digital electroencephalogram: dEEG)을 말하며 전문가적 판별력과 정확도를 갖춘 전문적 임상의에게 유용한 기법이라고 말할 수 있다. 그러나 전문가적 수준에 도달하려면 많은 노력과 경험 그리고 시간이 요구되며, 전문가라 할지라도 주관적 기준에 의존하기 때문에 편의(bias)가 개입될 소지가 있어서 때로는 유용성이 의문시되기도 한다. 최근 TM(topographic mapping) 기법을 필두로 하여, 정량적 뇌파분석기법(quantitative electroencephalogram: qEEG)들이 시각적 뇌파분석의 한계점들을 대체하고자 개발되어 도입되는 추세이다. 본고에서는 시각적 뇌파분석(dEEG)과 정량적 뇌파분석(qEEG)을 비교하여 그 장점을 취하면서 동시에 두 접근법의 한계점을 개선하여, 정확하고도 중요한 뇌파 정보가 잘 드러나는 통계적 뇌파분석법(statistical electroencephalogram: SEEG)을 대안으로 제시하고자 한다.

무증상 뇌경색의 위험요인에 대한 환자;대조군 연구

백혜기,고미미,유병찬,방옥선,오영선,김연진,김정현,김윤식,설인찬,Baek, Hye-Ki,Ko, Mi-Mi,Yu, Byeong-Chan,Bang, Ok-Sun,Oh, Yeong-Seon,Kim, Yeon-Jin,Kim, Jung-Hyun,Kim, Yoon-Sik,Seol, In-Chan 대한한방내과학회 2007 大韓韓方內科學會誌 Vol.28 No.4

Background : Cerebrovascular disease is a major cause of death and disability in adults. Silent cerebral infarction (SCI) portends more severe cerebral infarction or may lead to insidious progressive brain damage resulting in vascular dementia. Known cardiovascular risk factors, such as arterial hypertension, diabetes mellitus, smoking, hyperlipidemia and ischemic heart disease may increase the risk of SCI. This study was designed to evaluate the risk factors of SCI in an apparently normal adult population. Methods : We divided 340 neurologically normal adults (mean age=59.90$\pm$8.30, men:women = 146:194) who underwent brain computed tomography (CT) or magnetic resonance imaging (MRI) at the Stroke Medical Center in Daejeon University Oriental Medicine Hospital in two groups, Silent inf. and Controls,and analyzed risk factors of SCI by interview, physical examination and blood test. Risk factors of SCI were assessed by interview, physical examination and blood test. We performed Pearson's chi-square test and two-sample t-test for univariate analysis and multiple logistic regressions for multivariate analysis to evaluate risk factors of SCI. Results : Old age, diabetes mellitus, and high lactate dehydrogenase (LDH) levels were associated with SCI on univariate analysis. Diabetes mellitus was demonstrated to be an independent risk factor for SCI on multivariate analysis. Conclusions : Advanced age, diabetes mellitus, and LDH levels are associated with SCI.

어혈 진단 프로그램을 활용한 진단 실습 교육 만족도 연구

오용택,고미미,김영은,정지연 한의병리학회 2023 동의생리병리학회지 Vol.37 No.5

This study was conducted to search solutions to enhance the blood stasis diagnosis pattern differentiation program and improve the quality of Korean Medicine practice training courses by investigating student’s satisfaction using the web version blood stasis diagnosis program which is based on the previously developed blood stasis diagnosis questionnaire and adding criteria for choosing scales and pictures for additional information. During training courses for diagnosing blood stasis, students performed roles of being a doctor and a patient alternately by using the blood stasis diagnosis program as an educational material. Afterwards, students were to answer 11 questions in a Likert 5 scale questionnaire and a short-answer question to investigate the satisfaction of the blood stasis diagnosis program. The average value of 11 questions from the Likert 5 scale questionnaire resulted in a high level of 4.63. The highest ranking questions were questions asking 1) if the blood stasis diagnosis program is appropriate as an educational tool and 2) if the training course was satisfying using the blood stasis diagnosis program. Short-answer questions showed 78.9% positive feedback. Negative opinions such as the complexity of the questionnaire items and the need of additional criteria and visual material were included. The majority of students participating in the survey showed high satisfaction in the diagnosis practice training using the blood stasis diagnosis program. Based on the study, the blood stasis diagnosis program shows sufficient value for practical use if the program UI is improved and certain material is added.