학교, 그 본질의 회복을 위해

주종호 새가정사 2023 새가정 Vol.770 No.-

온도변화에 의한 cAMP 수용성 단백질(CRP)의 구조

주종호,구미자,강종백 한국생명과학회 2000 생명과학회지 Vol.10 No.3

cAMP 수용성 단백질인 CRP는 Escherichia coli에서 대사와 관련된 유전자의 전사를 조절한다. 본 연구는 야생형과 돌연변이 CRP 단백질의 열적 안정성과 온도에 따른 단백질의 구조변화를 관찰하기 위 하여 proteolytic digestion, UV spectrophotometer, CD spectrapolarimeter 등의 방법을 사용하였다. cAMP가 없을 때에는 야생형, S83G, S128A CRP가 열적 안정성에서 큰 차이를 보이지 않았지만, cAMP가 존재할 때 야생형 CRP가 다른 돌연변이 CRP보다 열적으로 더욱 안정함을 보였다. 그리고 protease digestion 실험을 통하여 높은 온도에서 cAMP의 존재와 무관하게 돌연변이 CRP에서 단백질 의 변성으로 인한 절단된 단백질띠를 관찰할 수 있었다. 그리고 55$^{\circ}C$에서 측정한 CD 스펙트럼에서 단백 질의 2차 구조인 $\alpha$-helix 구조가 부분적으로 파괴되었음이 관찰되었다. CRP (cyclic AMP receptor protein) regulate transcription of catabolite-sensitive genes in Escherichia coli. Wild-type and mutant CRP (S83G and S128A) proteins were used to measure the thermal stability and the temperature-dependent structural change by proteolytic digestion, UV spectrophotometer and CD spectrapolarimeter. The result indicated that wild-type CRP was more thermally stable than the mutant CRPs in the presence of cAMP. At a low temperature, wild-type CRP with cAMP was more sensitive to subtilisin than the mutant CRPs. At a high temperature, there was no difference of sensitivity to subtilisin among wild-type, S83G and S128A CRPs. CD spectra suggested that the secondary structure of CRP was destroyed partially at a high temperature.

두통으로 응급실에 온 신경학적 결손이 없으며 의식이 명료한 환자에서 3차원 뇌 전산화 단층 혈관 조영술의 이상 소견의 예측인자

주종호,우선희,이운정,최세민,경연영,정원중 대한응급의학회 2012 大韓應急醫學會誌 Vol.23 No.1

Purpose: Headache patients with an alert mental state and normal neurologic examination findings who visit the emergency department (ED) should be differentially diagnosed for the presence of cerebral vessel disease. Hence, the purpose of this study was to analyze the abnormal threedimensional cerebral computed tomographic angiography (3D-CTA) findings of mentally alert patients presenting headache, and investigate the clinical factors predictive of an intracranial abnormality. Methods: A total of 227 patients visiting the ED presenting headache and possessing an alert mental status were enrolled in this study and examined by 3D-CTA from January 2008 to December 2008. We compared the results of the 3D-CTA and the final clinical diagnosis for each patient. The patient participants were divided into two groups: an abnormal group, as confirmed by 3D-CTA, and a non-abnormal group. We compared the vital signs, past hypertension history, clinical manifestations, and the clinical factors predictive of abnormality between the two groups. Results: Of the total patients, 44 were identified with abnormal findings by non-enhanced CT, and 61 patients were identified with abnormal findings by 3D-CTA. SAH was found in 29 patients and unruptured aneurysm was discovered in 17 patients using 3D-CTA. The time interval between onset of headache to arrival at the ED was shorter in the SAH group (p=0.012), and sudden bursting headache was observed in 22 subarachnoid hemorrhage (SAH) patients (p<0.001). Statistically significant differences were observed between the two groups for symptoms of nausea, vomiting, neck stiffness and seizure. According to the results of the multivariate logistic regression analysis,sudden bursting headache and neck stiffness were independent predictable variables that affected the abnormal 3D-CTA group. According to the results of the multivariate logistic regression analysis, sudden bursting headache and neck stiffness were independent predictable variables for the abnormal 3D-CTA group. Conclusion: Sudden bursting headache, neck stiffness,vomiting, and advanced age were independent predictable variables observed in the abnormal 3D-CTA group.

석탄화력발전시설에서의 납, 카드뮴 분포특성

주종호 ( Jong-ho Joo ),문영훈 ( Young-hoon Moon ),이아영 ( A-yeoung Lee ),권도형 ( Do-hyung Kwon ),김영희 ( Younghee Kim ),석광설 ( Kwang-seol Seok ),송금주 ( Geum-ju Song ) 한국환경분석학회 2016 환경분석과 독성보건 Vol.19 No.4

Anthropogenic emission of lead (Pb) and cadmium (Cd) were assessed at tow power plants fueled by bitu-minous coal, and at one power plant fueled by mixes (bituminous and anthracite) coal. Plant input and output materials were sampled in each process. The input materials were the coal and B-C oil as fuel in the combustor, and limestone as the remover of SOx in air-pollutant control device. The output materials were bottom ash, fly ash, gesso and sludge. The materials were sampled at least three times at each sampling point, then analyzed using ICP or ICP/MS. The coal fuel was the main input source of Pb and Cd, and the concentration of Pb and Cd was higher in the mixed coal than in the bituminous coal. Total Pb and Cd emission in fly ash was highest among the output materials, and very low in off gas and sludge. However, the concentration of Pb and Cd in the sludge was highest among the output materials. The fly ash is sold for reuse, and the sludge is dumped in a landfill site. Therefore, Pb and Cd in the fly ash and in the sludge should be managed before they are passed on in these ways.

타목시펜 내성 유방암세포(T47D : A18/4-OHT)에서 trastuzumab과 ZD1389의 효과

박우찬,주종호,이동호,최승혜,김정수,오세정,송병주,정상설,전해명,이재학 대한외과학회 2004 대한외과학회 학술대회 초록집 Vol.2004 No.10

마우스피부암 발생과정에 있어서 텔로머레이저 활성에 관한 연구

강호일,지승완,주종호,황명실,염영나,이국경,김옥희 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

텔로머레이저는 진핵세포 염색체의 텔로미어를 합성하는 DNA 중합효소로서 정상세포에서는 발현되지 않지만 차부분의 악성종양에서 발현되고 있는 것으로 알려져 있다. 현재까지 형질변환 된 세표에서 무제한의 증식을 조절하는 효소로 알려져 있지만 텔로머레이저 활성화의 기전 및 조절인자들은 알려져 있지 않은 실정이다. 본 연구에서는 각기 다른 11전으로 발암촉진을 할 수 있는 4종류의 TPA,okadaic acid, anaralin 및 benzoyl perox겨e 발암촉진제를 사용하여 마우스 피부암을 운도한 후 이들 암 조직으로부터 텔로머레이저 활성을 측정함으로써 텔로머레이저f') 어떤 방식으로 재찰성화 되는가에 대한 단서를 추적하고 분자병리학적인 기법을 사용하여 조절기전을 찾고자 하였다. 븐 실험에서 사용 한 동물 발암모델은 다단계 발암과정을 비교적 용이하게 관찰할 수 있는 마우스 피부암 모델을 이웅하였다. 그 결과 TPA promotion 10주에서 22주까지 얻은 pap기oma 조직에서는 렐로머레이저 활성이 검출되었으며 TPA 대씬 benfoyl peroxide, okadaic acid, anthralin 으로 처리하여 얻은 papi13orna 조직(promotion 10주에서 22주까지)에서도 텔로머레이저 활성이 나타나는 것을 발견하였다. 그러나 TPA 를 포함하며 이들 발암축진제 등으로 처궈한 동일 주령의 papilloma 주위조직 및 acetone 만을 처리한 정상 피부조직아서늪 틸로머레아저 활성은 나타나지 않았다. 이상의 결과를 모두 종합하면 마우스 피부암 발생과정에사를 틸로머레이저 재활성화와 단백질 인산화와는 전혀 관련이 없는 것으로 추정되며 향후 텔로머레이저는 새로운 조기 암 진단지표로서 사용 가능성이 높다고 사료된다. Telomerase, a speciallzed RNA-directed BNA polymerase that extends telomeres of eukaryotic chromosomes, has acti)'ity in most malignant tumors and provides a mechanismfor the unlimited potential fo'S division of neoplastic celts. Although telornerase is known to hea regulated enzyme, the factors and mechanisms involved in telornerase regulation are not wellunderstood. In the present study, we compared the effect of 12-0-tetradecano?1-phorbol-13-acetate fTPA) and non-phorbol ester tumor promoters such as okadaic acid,anthralin and benzo!71 per()xide on the expression of telomerase in the mouse skincarcinogenesis system, a well characterized model for studying pre-malignant and malignantf-ogression. We found that most early papillomas harvested after 10 weeks of TPA promotionshowed telomerase activity- Other papillomas harvested after 10 weeks of okadaic acid,anthralin and benzoy3 peroxide promotion and after single treatment of DMBA only alsoshowed telomerase activity, 1-espec4ive3y. On the other hand, normal 3nd all skins surroundedby papiBlomas harvested after 10 11·eelts of these promo·ters has no telomerase activity. Takentogether these results, there .Bppears to be no clear association betwetn the level of telomeraseactivity and protein phosphor#lation in mouse skin papi31omas and telomerase may be useful asbio-markers in early detectiorl of tumors.

석탄화력발전시설에서의 TSP 및 PM10, PM2.5 배출특성

송금주,문영훈,주종호,이아영,이재복 한국환경분석학회 2018 환경분석과 독성보건 Vol.21 No.1

To quantify TSP, PM10 and PM2.5 emission characteristics, these parameters were sampled during all processes in a coal-fired power plant. Concentrations of TSP, PM10 and PM2.5 emitted from boiler were high, and the ratios of PM10/TSP and PM2.5 /TSP were very low; this result means that the boiler emitted more coarse particles than fine particles. The ESP removed most of the coarse particles. At the FGD inlet the concentrations of TSP, PM10 and PM2.5 were low, but the ratios of PM10/TSP and PM2.5/TSP were higher than at the SCR inlet. The ESP removed ~99.8%, of the particulates but only ~99.5% of fine particles (PM10) and ~97.3% of ultrafine particles (PM2.5). Among the TSPs emitted from the stack, the proportion of PM10 was 80-85% and of this, the proportion of PM2.5 was 47-56%. Therefore, this study demonstrates that the stack of the plant emitted mostly PM10 and PM2.5, and that the ESP as the dust remover installed in the plant should be improved to remove the fine particles

녹차 등에 의한 Peroxisome Proliferator-activated Receptor Transactivation의 변화 연구

이국경,조현영,주종호,김세일,황명실,염영나,강호일,김옥희 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

녹차는 동서양에서 오래 전부터 즐겄 마션오던 차이다. 암 예방효과가 있다고 여겨진 녹차가 랫드에서 과산화소체의 수와 관련효소인 paluitoyl CoA oxydase(PCoA)의 활성을 증가시킨다고 최근에 보고죄었다. 본 연군에서는 녹차에 의한 과산화소체의 증가간 peroxisome pro넓craters-activated receptor a (PPARO)의 활성을 통하여 일어나는 가를 알아보기 위하여 transient transfection assay법을 이용하였다. Cos-1 세포에 PP·ARu를 transfection 시킨 후 녹차, 홍차, 우롱차의 추출액과 녹차의 주성분인 epigallocatechin gallate (EGCG), epigallate catechin (EGC), epicatechin gallate (ECG),epicatechin (EC), gallic acid를 투여하였다. 또한 강력한 과산화소체 증가물질인 Wy-14,643과의 상호작용을 관찰하기 위하여 Wy-씨,643에 차 추출액 또는 성분들을 병용투여하였다. 녹차와 홍차 추출액과 EGCG(lOoM), EGC는 대조판에 비하여 PPARa의 활성을 1.5-2배 정도 증가시켰으며 차의 종류별로는 륵차>흥차>우롱차』둥글레차의 순으로 PPARC를 활성시켰다. 병용투여의 경우, 녹차는 Wy-14,643에 의한 PPARa의 활썽을 4-6배정도까지 강력하게 증가시켰다. 따라서 녹차는 PPARa에 직접적으로 작용하여 과산화소첸의 수를 증가시키는 것 이외에, 알려지지 않은 기전을 통하여 PPARa 의 활성조절에 관여하는 것으로 여겨진다. 또한 PPAaa의 찰성이 중간발효산물인 우롱차의 경우에는 낮은 반면 완전발효산물인 흥차에서 다시 높아지는 결과는 EGCG 이외에도 PPARa의 활성에 영향을 미치는 또 다른 물질이 존재함을 쩨시하고 있다. Tea is a number of peroxisomesnITnlber of oeroxisomespopular beverage. Recently it was reported that green tea increases the in rats. In this stfdy, to know that the rrlechanism to increase theanri activities of neroxilomal pnfvmpl il pxprrrf ihrourh activation of peroxisome proliferator-activated receptor u(PPARa), Cell trallsient transfection studies were carried out to investigate the interactions of tea extracts and major tea components with PPARa, cloned frorrl moust. Green tea and black tea extract, and epigallocatechlh gallate (ECCG), amajor component of fresh green tea leaves, slightly increased 1.5-2 times of the activatioR ofPPARC compared wiff DMEitf alone treatment. Especially the effective doses of tea extractsand EGCG were lower than doses necessary to EGCG-induced cell death and inhibition ofmetastasis by inhibiting urokinase activity In ether reports. Also at these concentrations, gieentea and bfack tea extracts enhanced the activation of PPARa induced by Wy-14,643(20 r M) ina dose dependent Tnanner. But even though oolong tea is semi-fermented during themanufacturing process, oolong tea extract showed less active than black tea_ These resvltsdemonshate that green tea possesses an unknown potent regulatory role in activation of PPARO byperoxisoine protiferators and some chemicals in addtion to EGCG may have role in thisregulation and exist iii the futl-fermented balck tea.

응급실로 내원한 급성 신우신염 여성 환자의 중증도 예측을 위한 호중구와 림프구의 수치비의 역할

송백호,박상현,소병학,김수현,주종호,최승필,오재훈 대한응급의학회 2020 대한응급의학회지 Vol.31 No.6

Objective: It is difficult to predict medical outcomes for acute pyelonephritis (APN) in women. A delay in diagnosis and treatment results in rapid progression to circulatory collapse, multiple organ failure, and death. We investigated the value of procalcitonin (PCT) and neutrophil-to-lymphocyte ratio (NLR) in APN patients hospitalized through the emergency room. Methods: We retrospectively evaluated women with APN presenting in the emergency room from January 2014 to May 2018. Inflammatory biomarkers, including PCT and NLR, were measured, and the severity of pyelonephritis was assessed using the Surviving Sepsis Campaign definitions (Sepsis-3). Multivariable logistic regression analysis was used to evaluate the risk factors associated with septic shock and the prediction for septic shock was compared using a receiver operating characteristic (ROC) curve. Results: A total of 357 female patients with APN were included. The median level of PCT and NLR was higher in the septic shock group compared with other groups. Multivariate logistic regression analysis showed that age and PCT were risk factors for septic shock. When the ROC curve of septic shock was compared, PCT showed a higher area under the curve than NLR (NLR 0.65 vs. PCT 0.80). Conclusion: The initial NLR in the emergency room showed significant differences depending on the severity as classified by Sepsis-3 definitions. However, NLR was not found to be associated with septic shock in female patients with APN.

플리스틱 가소제인 DIISOCECYL PHTHALATE 의 독성학적 고찰

이국경(Kook Kyung Lee),주종호(Jong Ho Joo),김세일(Se Yl Kim),제정환(Jeong Hwan Che),남기택(Ki Taek Nam),강진석(Jin Seok Kang),안병우(Byeong Woo Ahn),홍충만(Choong Man Hong),임국환(Kook Hwan Rhim),양기화(Ki Hwa Yang),장동덕(Dong Deuk 한국수의병리학회 2000 한국수의병리학회지 Vol.4 No.2