발표요지 : 인공종자 생산의 모델시스템 개발 Ⅲ. 당근의 전형적인 체세포배의 발생빈도에 미치는 sucrose 와 ABA 의 영향

정원중,송남희,양승균,이행순,장탁중,정경희,유장렬 한국식물생명공학회 ( 구 한국식물조직배양학회 ) 1989 한국식물생명공학회 춘계학술연구발표회 Vol.1989 No.-

오이 배발생세포의 현탁배양을 통한 고빈도 식물체 재분화

정원중,우제욱,박효근,최관삼,유장렬 한국식물생명공학회 1999 식물생명공학회지 Vol.26 No.4

Hypocotyl explants from 7 days old seedlings of one $F_1$ hybrid cultivar and two pure lines of cucumber formed embryogenic calli at frequencies of up to 8% when cultured on Murashige and Skoog medium (MS) supplemented with 1 mg/L 2,4-D for 3 weeks. Embryogenic calli gave rise to somatic embryos. When slices of somatic embryos were cultured on the same medium for 4 weeks, they formed embryogenic calli. Embryogenic cell suspension cultures were established with embryogenic calli in MS liquid medium with 1 mg/L 2,4-D. Embryogenic potential of cell suspension cultures was maintained by subculturing every seven days. When the level of 2,4-D in the medium was lowered to 0.2 mg/L by diluting with liquid MS basal medium, embryogenic cell suspension cultures underwent development into numerous somatic embryos. When plated onto MS basal medium, over 95% of somatic embryos developed into plantlets. Plantlets were transplanted to potting soil and grown to maturity. 발아 7일된 오이 (Cucumis sativus L.) F$_1$ 5품종과 순계 4품종의 하배축 절편을 1mg/L 2,4-D가 첨가된 MS고체배지에 치상하여 3주간 배양하였다. F$_1$ 1품종과 순계 2품종에서 발달한 캘러스의 8%이하에서 체세포배가 발생하였다. 체세포 배 절편을 동일한 배지에 4주간 배양하여 배발생캘러스를 유도하였다. 배발생캘러스는 동일조성의 MS액체배지에 현탁되어 증식하였다. 액체배지에서 배발생캘러스는 수많은 체세포 배로 발달하였으며, 생장조절제가 첨가되지 않은 MS기본배지에서 95%이상의 체세포배가 식물체로 발아하였다. 재분화된 식물체는 화분에서 생육되어 개화 후 착과하였다.

이송 모듈을 사용한 리플로우 오븐의 열유동해석

정원중,권현구,조형희,Jeong, Won-Jung,Kwon, Hyun-Goo,Cho, Hyung-Hee 한국반도체디스플레이기술학회 2006 반도체디스플레이기술학회지 Vol.5 No.3

Because of new requirements related to the employment of SMT(Surface Mounting Technology) manufacturing and the diversity of components on high density PCB(Printed Circuit Boards), Thermal control of the reflow process is required in order to achieve acceptable yields and reliability of SMT assemblies. Accurate control of the temperature distribution during the reflow process is one of the major requirements, especially in lead-free assembly. This study has been performed for reflow process using the commercial CFD(Computational Fluid Dynamics) tool for predicting flow and temperature distributions. Porous plate was installed to prevent leakage flow which was one of the major problem of temperature uniformity in the reflow process. There is a separation region where the flow is turned. Outside wall made of porous plate is to prevent and minimize separation region for acquiring uniform temperature during operation. This paper provided design concept from CFD results of the steady state temperature distribution and flow field inside a reflow oven.

Tombus 바이러스의 RNAi Suppressor p19 유전자에 의한 Chlamydomonas reinhardtii의 형질전환

정원중,유장렬,Heriberto Cerutti 한국식물생명공학회 2007 JOURNAL OF PLANT BIOTECHNOLOGY Vol.34 No.4

Chlamydomonas reinhardtii was transformed with the coding sequence of the Tombus virus gene p19 to determine whether the gene functions as an RNAi suppressor in C. reinhardtii. Transformants were confirmed to have 1 to several copies of p19 gene in their chromosomes. When an RNAi strain of C. reinhardtii generated by transforming the inverted repeat (IR) sequence homologous to the 3’UTR region of the MAA7 gene was re- transformed with the gene p19, MAA7 transcript levels of transformants fluctuated and proliferation of transformants on the medium containing 5-FI was suppressed. Overall results suggest that p19-mediated silencing suppression works at a low level in C. reinhardtii because of difference in codon usage resulting in weak P19 expression unless p19-mediated silencing suppression in C. reinhardtii works in a different manner from higher plants

형질전환 토마토에서 Cytosine Methylation에 의한 유전자발현 억제

정원중,정서희,민성란,이수영,박지영,S Javad Davarpanah,정화지,전재흥,유장렬 한국식물생명공학회 2007 JOURNAL OF PLANT BIOTECHNOLOGY Vol.34 No.4

Transgene expression was analyzed in tomato plants. Four lines of neomycin phosphotransferase II gene (NPTII) and the trehalose biosynthetic fusion gene (TPSP) transformed T0 plants showed kanamycin resistance on selection medium. However, the analysis of phenotype (kanamycin resistance) and mRNA expression in T1 plants indicated that the expression of the NPTII and TPSP transgenes was down-regulated to an undetectable level in two independent lines 1 and 11. Southern analysis demonstrated that the lines 1 and 11 had multicopies of the transgenes, whereas the typical transgenic lines 2 and 10 had 1 or 2 copies. DNA methylation analysis using methylation sensitive enzyme detected accumulated CpG DNA methylation on TPSP coding region and CaMV35S promoter region in the line 11, but not the typical transgenic line 2. These results suggest that multicopy transgene in plants is attributed to down-regulation of the transgene expression via transcriptional gene silencing.

이산화탄소 소화설비 오작동에 의한 이산화탄소 집단 중독

정원중,소병학,김형민 대한응급의학회 2012 大韓應急醫學會誌 Vol.23 No.2

Carbon dioxide is a colorless, odorless, nonirritating gas with many practical uses. In particular, because it can displace oxygen from the environment, it is used as a fire extinguisher. We describe an incident that occurred as a result of malfunction of a carbon dioxide-based fire extinguishing system in our hospital. Twelve casualties reached our emergency department. Symptoms of exposure included nausea, dizziness, loss of consciousness, vomiting,chest discomfort, and seizure. Results of initial arterial blood gas analysis showed acidosis in five patients. A new pneumonic infiltration at the left. upper lung field was observed in one patient, while sinus tachycardia in electrocardiography (ECG) was observed in another patient. Oxygen was initially supplied to all casualties, until symptoms of intoxication had disappeared. Three patients were admitted to the hospital,but were discharged without complication. Despite occurrence of massive casualties, with significant symptoms due to unintentional exposure to high concentrations of carbon dioxide, patients’ symptoms were relieved by supportive care.

벼의 수화겔 인공종자 생산

정원중,민성란,송남희,유장렬 한국식물생명공학회 1994 식물생명공학회지 Vol.21 No.3

태백벼의 현탁배양세포주로부터 유도된 체세포배를 낱개로 알진산 캡슐화하여 인공종자화하였다. 인공종자는 1/2 MS 고체배지에서 73%의 발아율을 나타내었으며 알진산 캡슐은 체세포배의 발아율에 영향을 주지 않았다. 그러나 멸균되지 않은 여과지에서는 발아율이 60%로 낮아졌다. 캡슐화된 진정종자는 무균상태여부에 관계없이 높은 발아율을 나타내었다. 이상의 결과로 무균상태가 유지되지 않을 때 인공종자 발아율이 낮아지는 것은 체세포배가 진정종자의 접합자배보다 오염을 견디기 어렵기 때문인 것으로 사료된다. Somatic embryos derived from cell suspension cultures of rice were singly alginate-encapsulated to be used as artificial seeds. When placed on half strength MS solid medium,73% of the encapsulated somatic embryos were capable of germination Encapsulation per se did not affect the germination frequency of embryos. When incubated by wrapping with moistured non-sterile filter paper, 60% of the encapsulated somatic embryos germinated. However encapsulated zygotic embryos without endosperm showed a high germination frequency regardless of the sterility of the incubation conditions. The results suggest that a greater susceptibility of somatic embryos to contaminants is attributed to lower germination frequency of encapsulated somatic embryos in non-sterile conditions.

벼 체세포배를 알긴산 캡슐에 넣어 제작한 건조형 인공종자

정원중,민성란,송남희,유장렬 한국식물생명공학회 1995 식물생명공학회지 Vol.22 No.1

벼의 인공종자를 무균상에서 건조시킴으로써 건조형 인공종자를 제조하였다. 1/2 MS배지에서 80%의 수분 손실률을 가진 인공종자는 20%가 발아하였다. 0.1 mg/L ABA가 첨가된 알긴산용액으로 제조한 인공종자는 0-90%의 수분손실률에서 최고 1.7배까지 발아율이 향상되었다. 이러한 결과는 ABA가 인공종자의 제조 및 건조과정에서 물리적 혹은 생리적인 저해에 대한 보호기능을 나타낸 것으로 사료된다. Dry-type artificial seeds were produced by dehydrating alginate-encapsulated somatic embryos of lire. When placed on half- strength MS solid medium 20% of the artificial seeds dehydrated to the level of 80% water loss were capable of germination. Addition of 0.1 mg/L ABA to alginate solution before encapsulation enhanced the germination percentage of those dehydrated to the level of nil to 90% water loss by up to 1.7-folds. The results suggest that ABA may enable somatic embryos to overcome physical and/or physiological restraint by encapsulation and dehydration.

적은 수의 거대 엽록체를 가진 핵 형질전환 식물체를 이용한 담배 엽록체 형질전환 빈도 제고

정원중,민성란,유장렬,Jeong, Won-Joong,Min, Sung-Ran,Liu, Jang-R. 한국식물생명공학회 2007 식물생명공학회지 Vol.34 No.3

In the chloroplast transformation process, a chloroplast containing transformed chloroplast genome copies should be selected over wild-type chloroplasts on selection medium. It is more effective for a cell to become homoplasmic if the cell contains smaller number of chloroplasts. Therefore, to reduce the number of chloroplasts in mesophyll cells in tobacco, we overexpressed FtsZ to generate transgenic plants, of which mesophyll cell contained a few enlarged chloroplasts contrast to a wild-type mesophyll cell containing approximately 100 chloroplasts. It was demonstrated that transgenic leaf tissues comprising cells with a few enlarged chloroplasts gave rise to approximately 40% higher frequency of chloroplast-transformed adventitious shoots. 엽록체 형질전환된 식물체를 얻으려면 먼저 세포수준에서 모든 엽록체가 형질전환되어야 하는데, 세포내에는 많은 수의 엽록체가 존재하므로, 엽록체 형질전환 벡터가 전이되어 형질전환된 엽록체는 선발배지에서 선택적으로 분열을 계속하고 형질전환되지 않은 엽록체들은 분열을 하지 못하게 되어, 결국 해당 세포내의 모든 엽록체가 형질전환된 상태에 이르게 된다. 따라서 만일 해당 세포내에 엽록체의 수가 적으면 그만큼 효율적으로 엽록체 형질전환을 할 수 있을 것이다. 본 연구에서는 담배의 FtsZ 유전자를 핵형질전환법으로 과잉 발현시킴으로써 엽록체의 분열이 저해되어 엽육세포내에 거대한 엽록체 3-5개를 가진 담배식물체의 엽육조직을 이용하여, 엽록체 형질전환을 한 결과, 엽록체 형질전환 빈도가 약 40% 증가되었다.

글리포세이트 계 제초제 음독 환자의 응급실 초기 검사에 따른 예후 예측

정원중,박규남,이원재,소병학,최세민,이미진,최경호 대한응급의학회 2006 대한응급의학회지 Vol.17 No.6

Purpose: Glyphosate is a widely used herbicide and has been considered to have low mammalian toxicity. However, a number of reports have described an increasing frequency of glyphosate poisoning with severe associated complications. The purpose of this study is to indentify predictors of serious complications based on initial findings. Methods: This retrospective study was performed from January 1, 2000 to March 31, 2006 at four university hospital emergency departments in Seoul and Kyung-Gi Do, Korea. Thirty-six patients were classified into two groups according to severity of symptoms. Initial clinical and laboratory findings were reviewed. Result: Glasgow coma scale (GCS) scores of severe poisoning group (SP, n=13) were significantly lower than those of mild poisoning group (MP, n=23). White blood cell (WBC) counts and serum creatinine concentrations were significantly higher in the SP group. WBC count, GCS score, serum amylase and creatinine concentrations were significant prognostic predictors as indicated by univariate logistic regression analysis. By multivariate analysis, two predictors were identified: serum amylase concentration (odds ratio=1.015) and serum creatinine concentration (odds ratio=357.309). Conclusion: In managing cases of poisoning from glyphosate-containing herbicides, clinical and laboratory findings must be carefully evaluated. Serum amylase and creatinine concentrations are especially important for quickly determing which patients require intensive care.