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- 저자 : 이국경(Kook Kyung Lee) (검색결과 11 건)
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랫드에서 계피유래활성물질(CB-PH)의 경구투여에 의한 4주간 반복투여독성 시험
조현무(Hyun-Mu Jo),성낙원(Nak-Won Seong),제정환(Jung Hwan Che),박기대(Ki Dae Park),남기택(Ki Taek Nam),조완섭(Wan-Seob Cho),한범석(Beom Seok Han),양기화(Ki Hwa Yang),김방현(Bang Hyun Kim),이국경(Kook Kyung Lee),김형진(Hyoung-Chin Kim 한국독성학회 2003 Toxicological Research Vol.19 No.4
Although 'Cinnamon' has been widely used for the food and biophamacy in the world, it's toxicity was not screened completely. Major component of 'cinnamon' is CB-OH and CB-PH. CBPH has been reported to have antimutagenic effect. To investigate the toxicity of 2- o-Benzoylcinnamaldehyde (CB-PH), repeated dose (4 weeks) oral toxicity test performed in SD rats. Results of repeated dose oral toxicity tests for 4 weeks (CB-PH; 500, 1000, 2000 mg/kg/day) suggested that the CB-PH treated group showed no significant toxicological findings with body weights, organ weights, hematological and histopathological findings. Therefore, these data indicated that the maximum tolerated dose of CB-PH was 2000 mg above/kg/day in the rats.
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이국경,양원형,박원근,송태웅,허창복,박전홍,홍용원,김송학,오찬우 濟州大學校亞熱帶農業硏究所 2004 아열대농업생명과학연구지 Vol.20 No.1
Managing, processing, and expressing the data, the database system makes users save the time and labors required to look up and organize the useful data as well as helps to create some new information and ideas. However in Korean situation, our the history of research is not too long and the resources including database systems is not enough. Because other previous database svstems manage the data in a form of text file. I t is not easy to obtain a useful analyzed results at once. The goals of this project are to extract experimental data from the research on endocrine disruptors. to manage the data as a code form, to process the data, to create the useful analyzed results, to help toxicological researchers. and eventually to enhance the availability of raw data. Before the systems design, we constructed the code tables from the reports and executed a pilot test. Depending upon the result of pilot test, we determined the factors to be extracted as data set from reports. constructed database tables, and designed the software to manage the data-input and to search and analyze data, Data-input module help users easily to analyze the report in a paper form and to input the data. Developed in the web-based form, data-search module is able to give an access to public as well a s researchers. Also depending on the data saved in a code form. data-search module can provide much useful information and have a potentiation to be developed as more powerful data-analyzing system.
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Peroxisome proliferator에 의해 발현 억제되는 유전자의 검색
이국경,강호일,김세일,염영나,정자영,황명실,김옥희 식품의약품안전청 1998 식품의약품안전청 연보 Vol.2 No.-
설치류예서 peroxisome pl·oliferator(PPs)의 장기투여는 간세포의 증식을 일으키며 이에 따른 간장 무게의 증가를 유발한다. 이후 간장 무게의 증가된 상태가 계속 유지되다가 수개월 후에 간암을 발생한다. 본 실험은 PPs에 의하여 간장 무게가 증가된 간세포의 상태를 유전자 발현순준에서 이해하기 위하여 dfferential display-reverse tran scrlption-polymerase chairl reaction(DD-HT-PCR) 기법을 이용하먼 발현이 감소된 유전자를 검색하였다. 이에 82개의 발현 감소가 예측되는 CBNA 절편을 분리하였고 reverse northerB blot으로 9종윽 유전자가 발현 감소됨을 확인할 수 있었다. 9종의 선별된 CDNA 절편의 염기서열을 확인한 곁과 2종은 transferrin과 of-inhibifur 111에 대해 각각 100%읜 동질성을 갖는 CDNA 절편이었으며, 또 다른 2종의 CDNA 절펀은 long Inter spersed repetitive nuclear element(LINE)와 98%의 동질성을 보였단. 나떠지 5종윽 CDNA 절편은 매우 낮은 농질성을 보이거나 꿀색이 되지 않았다. Transferrin의 감소는 peroxisome pro?iferator actiuated receptor(PPAR)가 transferrin의 transcription factor인 HNF-4(hepatocyte nuclear factor-4)와 경쟁적인 작용함에 기인한다.본 실험에서 PPs에 의해 발현 감소되는 것으로 처음 밝혀진 α1-inhibitor III는 종양세포에서 발현이 감소하는 유전자로 알려져 잇으며 또한 역시 PPs에 의해 발현감소되는 retrotransposon인 LINE과 아직 동정되지 않은 5종의 유전자들의 발현 감소는 PPs의 작용범위가 알려진 범위보다 넓을수 있음을 제시하고 있다. 따라서 PPs는 현재까진 알려진 transcription factor외에도 다른 transcription factor와 경쟁적으로 aL울괴거나 또는 다른 경로를 통하여 단백질의 발현에 관여함으로써 간세포의 증식 또는 대사에 영향을 끼칠 수 있을 것으로 여겨진다. Peroxisome proliferators (PPs) increase the liver weight. maintain the increased weight of liver. and eventuallf influce liver tumor in rodents. To understand the hyferplastic status of liver in-duced by long-terrE treatuent of PPs, the techaique ef differential display-reverse transcription-poly-morass chain reaction (D3)-RT-PCR ) was rerformed to detect the transcriptionally down-regulatedgenes that would be relateil te the homeostasis or altered function of rat liver treated with PPs. To iso-late HNA from rat liver maintaining increased weight, the rats were given 0.5% clofibrate-containingdiet for 3 weeks. Using DD~RT-PCR teehnique,82 suspected CDNA fragnlents shewing transcriptionallydown-regulated patterns were isolated. Only 9 CDNA fragments aiuoBg these fragraents were decreasedtranscriftionally by 4 other PPs (Wy-14,643, di(2-ethylhexyl) phthalate, di(2-ethylhexyl) adipate, andperchloroethylene). Two CHNA fragments have 100% similarity wi·th transferrin and α1-inhibitor lIt,respectively. Two other CBNA fragmeBts have 98% sirailarity with long interspersed repetitive nuclearelements (LINE). The expression of transferrin was already knowB to be regulated by HNF-4(hepatecyte nuclear factor-4) and decreased by competitive action of peroxisome proliferator-activated.scepter :fPAR) . But the t.anscriptionally down-.egulatioa of of-inhibitor lIT by PPs was firstlyshown in-tliis study. The transcriptional reductions of a nlunber of LINE interspersed within raammali-an genome and the unidentified genes imply that the scope of genomic targets by PPs may be more enor-mous than what we have krㄴown. Therefore these results svggest that PPs may have an influence on cetlcycle or homeostasis througt competitive action against transcription factors or through other pathway.
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정자영,이국경,염영나,이종근,김현배,김세일,강호일,이영순,김옥희 식품의약품안전청 1997 식품의약품안전청 연보 Vol.1 No.-
This study was enamined on the effect of adoptive immunotherapy in T,12-dimethylbena la]aathracene(B?BA) -in13uce6 rat mammary carcinogenesis. Flfty- sik-day-old female Sprague-Dawley 40 rats were divided into four groups. fLs a positive controtp animals in Group I were intubated with DMBA. Smg/100g body weight and single dose, at onset of experimeat. Group ll was treatedadoptive iinrnunotherapy with polyinosinic- polycytidylic acid(Poly I:C) and Group 111 was treated withInterleultin(IL-2). Group nr is negative control, therefore no treated group. All animals were sacrificedat 16 weeks after DMBA in.tubation. Mammary gland wet weight, dry fat free tissue weight, incidence oftumor, and number of lobules, alveolar buds, terminal end buds, ftnd terminal ducts were examined,Histopathogenesis of DMBA-iudvced rat mammary tumors were performed by whole mount method androutine histologic technique. As a result, the induced mammary tumors of Group I was 60%. The effectof adoptive irnrnunotherapy in DMBA-induced rat mammary carcin☞genesis of Group rl and ITI were 33% and 0%, respectively. Histopathological types of induced-mammary tumors were adeaorna,adenocarciaoma and carcinosarcoma. Morphological changes of the mammary gland after treated withDMBA was analysed by whole mount and histopathological methul- The number of the terminal endbuds and_terminal ducts are significantly higer in DMBA treated Groups(p<0.01) than non-treatedGroup ini fbservation of wllole mount method. Tn microscopy observation, lobules containing alveolarbuds and hyperplastic alueolar nodules were significantly higher in DMBA treated Groups(p<0.01)than aon- treated Group. Especially, hyperplastic alveolar nodules is very sensitive parameter to assessthe mamraary carcinogenesis and the effect of adoptive immunotherapy.
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Nicotine 및 Tobacco-specific nitrosamine이 발암과정에 미치는 영향
강호일,황명실,김은정,김윤정,이국경,정자영,원도희,김옥희 식품의약품안전청 1998 식품의약품안전청 연보 Vol.2 No.-
Nicotine은 그동안 계암발생파정에 관여되어 있는 것으로 추정되어지고 있으나 현재 이에 대한 기전.은 밝혀진 것이 거의 없다.본 연구에서는 폐암발생과정에서 있어서 nicotine의 역할을 조사하기 위해 첫번째 실험군으로 nicotine을 Sprague-Dawley 랫드에 연속적으로 10ㅇ리간 투여한 후 암유전자 및 암억제유전자의 발현변화를 단백질과 mRNA레벨에서 검토하엿다. 그 결과 5종류의 암유전자인 ras,raf,myc,fos, jun,및 2종류의 암억제유전자인 p53,Rb 단백질의 발현변화는 거의 없는것으로 나타났다.그리고 2종류의 암유전자인 myc,fos및 암억제유전자 p53 mRNA발현변화 역시 거의 없어 단백질 발현 실험결과와 일치하는 것으로 나타났다. 두번째 실험군으로 nicotine 및 sodium nitrite그리고 NNK를 Fischer344 랫드에 투여하여 발암물질인 NNK의 생성여부를 검토한 결과 NNK를 단독투여한 실험군의 경우 8-OHdG 레벨이 1.8배에서 2.3배까진 현저하게 증가하였으나 nicotine 및 sodium nitrite를 단독 혹은 병용 투여한 경우 8-OHdG 레벨의 유의한 차이가 관찰되지 않아 NNK 생성을 확인할수 없었다. Nicotine has been implicated as a potential factor in the pathogenesis of human lung cancer, however its mechanism of action in the development of lllng cancer remains largely unknown.To explore the role of Bicotine in the developmene of lung cancer, lue first investigated the effects of nic-otine on the expression of tumor associated genes by treating Spragve-Barley rats with nirotine (10 mf/kg) by gavage once daily for 10 days. We determined the expression of proteins and rnRNAs of the raf,raf, mrt JHn, foa oncogenes and p53, ab tumor suppressor genes by Western and Northern blotting,respectively. We did not detect any changes on t31e levels of prote·ins aBO mRNAs of these tumor associ-ated genes in the lung of Sprague-Dawley rats from 3 days to 12 ·weeks after the last treatment of nico-tine, indicating that nicotine appears to have no effect on the exE)ression of these oncogenes aBd turnersuppressor genes at an early stage in multistage chemical carcinoifenesis. In a second experiment, we in-vestigated the possibility that 4-(methylnitrosamino)- t- (3-p!rridyl)-1-butaaone (NNK) could beformed eBdogenously bf treating with nicotine and sodium nitrite. We treated groups of Fischer 344 ratswith nicetiBe (60 rrnol/kg) and sodium nitrite (180 rmol/kg), nicotiae, sodiu:n-nitrite and NNK (120 #mol/kg) flfne by gavage once daily to, 7 dafs, .espectively and dete,mined the 8-hydroxy-deoByguanosine (8-OHdG), as an indicator of NNK formation, in the lungs of rats 24 hours and 48hours after the last treatmeilt by HPLC/ECD method. We detect 3ncreased level of 8-OHdG in the tungsof rats treated with NNK, but in the case of nicotine plus sodiLlra nitrite, nicotine and sodium nitritealone we could not detected any changes of 8-OHdG, respectively.
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