식용 버섯의 조리방법에 따른 Jurkat 세포주 DNA 손상 보호 효과

조윤정,김경희,육홍선,Cho, Yun-Jeong,Kim, Kyoung-Hee,Yook, Hong-Sun 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.1

본 연구는 식용 버섯의 조리방법에 따른 항산화 생리활성의 평가를 위해 수행되었으며, 산화적 스트레스에 의한 DNA 손상 감소 효과를 통해 조리방법을 달리한 버섯 추출물의 유전독성학적 방호효과를 살펴보았다. Human lymphocyte에 조리방법을 달리한 3가지 버섯(느타리, 팽이, 표고)의 추출물을 처리하고, hydrogen peroxide($H_2O_2$)로 산화적 손상을 준 후, DNA 감소 효과를 Comet assay로 평가한 결과, 모든 시료군에서 산화적 손상에 의한 DNA 손상 감소 효과를 나타냈다. 3가지 버섯 모두 비조리군이 조리군보다 높은 효과를 나타냈는데, 이는 조리과정에 의한 페놀성 화합물의 감소로 인한 것으로 보이며, 조리군 중에서 볶기와 전이 비교적 낮은 DNA 손상 감소 효과를 나타낸 것은 조리 시 첨가되었던 대두유의 가열 산화에 의한 것으로 사료된다. 결론적으로, 조리된 버섯은 생버섯에 비해 산화적 스트레스에 의한 DNA 손상 감소효과가 낮으나, 양성 대조군과 비교하였을 때 손상을 유의적으로 감소시킨 것으로 나타났다. 또한, 본 연구에서 사용한 네 가지 조리법(굽기, 데치기, 볶기, 전) 중 DNA 손상 감소에 효과적인 조리법은 대두유를 사용하지 않은 굽기와 데치기인 것으로 판단된다. In this study, portective effect on DNA damage several mushrooms (Pleurotus ostreatus, Flammulina velutipes, Lentinula edodes) according to cooking methods was investigated using Comet assay. Three edible mushrooms were cooked by grilling, blanching, pan-frying, or by preparing 'Jeon' (traditional Korean pancake). Cells were incubated in medium with 4 kinds of samples for 48 h ($37^{\circ}C$) were further treated with hydrogen peroxide ($H_2O_2$) for 5 min as an oxidative stimulus. Oxidative damage was evaluated by single-cell gel electrophoresis (Comet assay) and quantified by tail DNA% (TD), tail length (TL), tail moment (TM). Though oxidative DNA damages expressed as TD, TL, TM of 4 cooked samples were higher than raw sample, which means lower protective activities, all samples including raw sample had significantly higher protective effects than the positive control (p<0.05). The protective effect on DNA damage of cooked samples decreased much more when soybean oil added, likely due to the thermal oxidation of oil during cooking. Although heat treatment could degrade protective effect on DNA damage of mushrooms, the cooked mushroom had significant effect on oxidative stress. In conclusion, grilling and blanching were the most advantageous cooking methods to protect oxidative DNA damage induced by $H_2O_2$.

갑상선 미세 유두암에서 종양 크기의 구분에 따른 임상병리학적 특징의 분석

조윤정,이동호,이상철,김세준,김정구,안창준,이관주,Yun-Jung Cho,M,D,Dong-Ho Lee,M,D,Sang-Chul Lee,M,D,Say-Jun Kim,M,D,Jung-Koo Kim,M,D,Chang-Joon Ahn,M,D,and Kwan-Ju Lee,M,D 대한갑상선-내분비외과학회 2010 The Koreran journal of Endocrine Surgery Vol.10 No.3

Purpose: Although the detected incidence of papillary thyroid microcarcinoma (PTMC) has increased with development of ultrasonography and fine-needle aspiration biopsy, the best treatment has not yet been established. Treatment decisions require information on many factors including lymph node metastasis, extrathyroidal extension, and bilaterality. With this aim, the present study analyzed clinicopathologic features of PTMC according to cut-off of tumor size. Methods: The clinicopathologic features of patients with PTMC between January 2007 and December 2009 were reviewed retrospectively from medical records. Patients were divided according to tumors lesser than or equal to cut-off (Group I) and tumors exceeding cut-off (Group II). Results: Both capsule invasion and lymphovascular invasion were significantly different at all cut-off diameters (5∼9 mm). Central node metastasis revealed a difference in all cut-off values except 8 mm. Extrathyroidal extension differed at all cut-off values except 5 mm. Bilaterality displayed a statistically significantdifference only at the 8 mm cut-off. Conclusion: A cut-off of 5 mm represents a safe value to discriminate less aggressive from aggressive treatment for PTMC. (Korean J Endocrine Surg 2010;10:152-156)

출혈우세병변이 동반된 연령관련 황반변성 환자에서의 유리체강 내 라니비주맙 주입술의 효과

조윤정,박성표,Yoon Jung Cho,Sung Pyo Park 대한안과학회 2011 대한안과학회지 Vol.52 No.7

Purpose: To report the efficacy and safety of intravitreal ranibizumab monotherapy in patients with age-related macular degeneration with a predominantly hemorrhagic lesion. Methods: Nineteen eyes of 19 patients with submacular hemorrhage involving the fovea were treated with intravitreal ranibizumab (0.5 mg/0.05 ml) injections as needed. All patients completed at least 4 months of follow-up. Ophthalmologic examinations, fluorescence angiographic evaluations, optical coherence tomography (OCT) examination, and hemorrhage size were analyzed before the injections, and at 1,2,4,6, and 12 months follow-up. Results: The average VA and mean central retinal thickness (CRT) before ranibizumab treatment were 0.62 ± 0.39 log MAR and 335.76 ± 111.22 μm, respectively. Additionally, the VA and CRT four months after the initial injections were 0.54 ± 0.43 log MAR and 241.42 ± 107.55 μm, respectively. The mean size of the hemorrhage was significantly reduced from 2.87 ± 2.44 DA (disk areas) at baseline to 0.9 ± 1.28 DA at four month follow up. Conclusions: Intravitreal injection of ranibizumab is an effective treatment option for patients with age-related macular degeneration with a predominantly hemorrhagic lesion. J Korean Ophthalmol Soc 2011;52(7):838-845

Depressive Mood and the Risk of Future Functional Decline in an Elderly Population

조윤정,홍영호,박형준,이지은,윤재문,신동욱,손기영,Be-Long Cho,김상혁 대한노인병학회 2017 Annals of geriatric medicine and research Vol.21 No.3

Background: As the elderly population increases in number, prediction and prevention of functional decline in this population are of great importance. This study evaluated whether depression in an elderly population could predict functional decline in activities of daily living and the components of instrumental activities of daily living 2 years after baseline. Methods: Data from the Korean Longitudinal Study of Aging panel, a national, representative sample of aging Koreans, were used. The subjects were the elderly aged 65 years or older who had no disabilities that hindered them from performing activities of daily living at baseline. Depression was measured using the Short-form (10-item) Center for Epidemiological Studies Depression Scale. Multivariable logistic regression analysis was performed to evaluate the effect of functional impairment according to baseline depression. Results: Of the 3,477 participants, 166 (4.8%) showed at least 1 impairment in the 7 activities of daily living (ADL) categories after 2 years. The participants with depression (scored 15 or higher on the depression scale) were more likely to have functional decline 2 years later (odds ratio, 1.82; 95% confidence interval, 1.23-2.71). All the components of the ADL had an increased tendency to develop functional decline (all odds ratios>1.00). Furthermore, negative items were significantly associated with functional decline (all p-values adjusted for trend<0.05). Conclusion: The participants with depression were more likely to have functional decline 2 years later. Such decline was observed in all types of ADL. Therefore, depression should be monitored and used as a predictor of functional decline in elderly adults.

변형된 광역학 요법으로 치료한 유방의 침윤성 관상암종의 맥락막 전이 1예

조윤정,박성표,Yoon Jung Cho,MD,Sung Pyo Park,MD 대한안과학회 2010 대한안과학회지 Vol.51 No.3

Purpose: To report a case of choroidal metastasis of breast cancer that was treated with modified photodynamic therapy. Case summary: A 45-year-old woman visited our clinic with blurred vision of the right eye, which began 1 month before. The patient previously suffered from a low back pain for 1 year. The best corrected visual acuity was 20/20 in both eyes. Fundus examination revealed an elevated yellowish mass-like lesion at the superonasal area in the right eye. Ultrasonography of the right eye showed a highly echogenic choroidal mass with moderate to high internal reflectivity. Fluorescein angiography showed hypofluorescence during the prearterial and arteriovenous phase, and well circumscribed hyperfluorescence during the venous phase. Radiologic examination was performed upon suspicion of metastasis. The examination revealed breast cancer with lung, spine and ovary metastasis. Subsequently, biopsy of the breast mass revealed an invasive ductal carcinoma. Based on these results, the patient was diagnosed with choroidal metastasis from breast carcinoma. The patient received systemic chemotherapy, and modified photodynamic therapy (PDT) was performed on the metastatic choroidal mass. Six days after modified PDT, the mass size was unchanged, and serous retinal detachment developed at the macula and inferior retina. However, 22days after treatment, the mass size markedly decreased and the serous retinal detachment was improved. J Korean Ophthalmol Soc 2010;51(3):458-462

점막하 침윤 조기위암 환자에서 VEGF-C와 COX-2 발현의 임상적 의의

조윤정,이정의,이관주,박조현,박승만,전해명,안창준,김정구,이동호,이상철,Cho, Yun-Jung,Lee, Jung-Uee,Lee, Kwan-Ju,Park, Cho-Hyun,Park, Seung-Man,Jeon, Hae-Myung,Ahn, Chang-Joon,Kim, Jeong-Goo,Lee, Dong-Ho,Lee, Sang-Chul 대한위암학회 2009 대한위암학회지 Vol.9 No.3

목적: Vascular endothelial growth factor (VEGF)-C와 -D 및 Cyclooxygenase (COX)-2는 위암에서 림프절 전이와 연관이 있다고 알려져 있다. 이에 저자들은 점막하 침윤 조기위암에서 VEGF-C와 -D 및 COX-2의 발현과 림프절 전이 등을 포함하는 다양한 임상병리학적 인자와의 관련성을 알아 보고자 하였다. 대상 및 방법: 1991년 1월부터 2007년 10월까지 본원에서 점막하 침윤 조기위암으로 수술을 시행 받은 85명의 환자를 대상으로 VEGF-C, -D 및 COX-2와 VEGF-C에 대한 면역 조직화학 염색을 시행하였다. 염색의 결과에 따라 환자군을 나누어 다양한 임상병리학적 인자와의 연관성을 조사하였고, 또 이 세 가지 인자들 상호 간의 연관 관계를 분석하였다. 결과: 전체 85명의 환자 중 16명이 림프절 전이가 있었다(18.8%). VEGF-C는 34.1% VEGF-D는 22.3% 그리고 COX-2는 37.6%가 양성으로 판정되었다. 이 중 VEGF-C와 COX-2 모두 림프절 전이와 유의한 상관관계를 보였고(P<0.001, P=0.023). VEGF-D와 연관성을 보이는 인자는 확인하지 못하였다. 또 VEGF-C와 COX-2의 발현은 밀접한 상관관계를 보였다(P=0.001). 결론: 점막하 침윤 조기위암에서 VEGF-C와 COX-2는 림프절 전이와 연관이 있고, 따라서 이 두 인자가 점막하 침윤 조기위암의 림프절 전이를 예측하는 인자로서의 가능성이 있다고 할 수 있겠다. Purpose: Lymph node metastasis is an important factor in determining prognosis and therapeutic options for early gastric cancer (EGC) patients. Vascular endothelial growth factor (VEGF)-C and D are known as lymphangiogenic factors, and cyclooxygenase (COX)-2 is thought to play a role in lymph node metastasis in gastric carcinoma. This study was designed to determine whether the expression of VEGF-C, VEGF-D, and COX-2 is associated with clinicopathologic factors, especially lymph node metastasis in EGCs invading the submucosa. Materials and Methods: Tissue samples were obtained from 85 Patients undergoing standard gastrectomy with lymph node dissection between 1991 and 2007 in the Department of Surgery of Daejeon St. Mary's Hospital in Daejeon, Korea. All patients were diagnosed with gastric cancers and submucosal invasion. We examined the expression of VEGF-C, VEGF-D, and COX-2 using immunohistochemical methods. Results: Of the 85 patients, 16 (18.8%) had lymph node metastasis. VEGF-C, VEGF-D, and COX-2 were positively expressed in 34.1% (29/85), 22.3% (19/85), and 37.6% (32/85) of the patients. VEGF-C and COX-2 expression was significantly correlated with lymph node metastasis (P<0.05). A positive correlation existed between VEGF-C and COX-2 expression (P< 0.001). Conclusion: VEGF-C and COX-2 expression is associated with lymph node metastasis in gastric cancer with submucosal invasion. VEGF-C and COX-2 may thus be predictive markers for lymph node metastasis in EGC patients with submucosal invasion.

폐경기 한국 여성에서 운동 빈도와 스트레스와의 상관관계

조윤정(Yoon Jeong Cho),이건호(Geon Ho Lee) 대한스트레스학회 2016 스트레스硏究 Vol.24 No.3

본 연구는 폐경기 여성에서의 일상생활의 스트레스와 운동 횟수와의 연관성 여부에 알아보기 위해 시행하였다. 2005년 1월에서 2009년 12월 사이 대구의 한 대학병원에서 건강검진을 위해 내원한 수검자중 폐경 여성 1,883명을 대상으로 분석하였다. 스트레스의 척도는 심리사회적 웰빙지표 단축형(Pshchosocial well being index short form, PWI-SF)을 사용하였으며 연령, 교육수준, 사회경제적 상태 및 음주, 흡연, 신체적 활동을 포함하여 조사하였다. 운동횟수는 일주일에 0회, 1∼2회, 3∼4회, 5회 이상으로 나누었다. PWI-SF 총 점수를 구하여 8점 이상을 건강군, 9∼26점을 잠재적 스트레스군 그리고 27점 이상을 고위험군으로 나누어 분석하였다. 통계 분석은 카이제곱 검정, 일원배치분산분석, 공분산분석을 이용하였다. 본 연구 결과 PWI-SF 점수와 운동 빈도는 유의한 음의 상관관계를 보였다. 연령, 흡연, 음주, 교육수준, 소득수준, 결혼여부, 약물치료 여부를 보정한 결과에서도 주당 운동빈도가 높을수록 스트레스 점수가 낮은 결과를 보였다. 폐경기 여성에서 주당 운동 횟수가 많을수록 PWI-SF 점수가 낮아, 유의한 음의 상관관계를 보여주었다. This study was conducted to understand the relationship between stress and exercise frequency in postmenopausal women of Korea. The participants were 1,883 postmenopausal women who had visited a single medical center in Daegu, between 2005 January to 2009 December. We excluded women who had been previously diagnosed depressive disorders or had been treating in hormonal replacement therapy. We used PWI-SF (psychosocial well being index short form) for measuring stress. The sum of PWI-SF score was classified into healthy (less than 8), latently stressed (9∼26) and high risk stressed (more than 27) groups. The frequency of exercise was divided into none, mild (1∼2 times a week), moderate (3∼4 times a week) and intense (more than 5 times a week) groups. We analyzed the data by utilizing chi-square test, ANOVA and ANCOVA. Our study showed a significant negative correlation between PWI-SF and frequency of exercise. Furthermore our result showed a statistically significant correlation between level of education, income and stress, while alcohol and smoking were not significantly associated with PWI-SF. Our result demonstrated that higher frequency of exercise was associated with low PWI-SF score in postmenopausal women of Korea.

유행선도력에 따른 소비자 세분집단의 심리적 특성과 의복추구혜택 특성

조윤정(Youn Jung Cho),고애란(Ae Ran Koh) 한국소비자학회 2001 소비자학연구 Vol.12 No.3

The purposes of this study were 1) to subdivide female consumers according to fashion innovativeness and fashion opinion leadership and identify the differences of demographic characteristics among the subdivided groups, 2) to identify the differences of personal characteristics, such as optimal stimulation level, materialism and values among the subdivided groups by fashion leadership and 3) to identify the clothing buying behavior, such as clothing benefits sought, fashion selection behavior and actual state of clothing shopping, among the subdivided groups. The data were collected via a questionnaire from 359 married female of ages 20`s∼40`s living in Seoul and suburban areas. The results of this study were as follows : 1) Female consumers were subdivided into innovative communicators, fashion innovators, fashion opinion leaders, fashion followers and fashion indifferents. And there were significant differences among subdivided groups with respect to demographic variables. 2) Optimal stimulation level and materialism were proven to be significantly different among subdivided groups by fashion leadership. 3) Seven dimensions of values were identified through factor analysis: consumption-oriented, achievement-oriented, diligence/sincerity-oriented, pessimistic value tendencious, conservative/conventional value oriented, dignity-oriented and sociality-oriented. And when it comes to the subdivided groups by the fashion leadership, 5 values factors such as consumption-oriented, achievement-oriented, diligence/sincerity-oriented. conservative/conventional value oriented, and sociality-oriented were proven to be significantly different. 4) Five dimensions of clothing benefits sought were identified through factor analysis: self-expression/individuality, brand-value, price-consciousness, decency and quality. And when it comes to the subdivided groups by the fashion leadership, 4 clothing benefits sought factors such as self-expression/individuality, brand-value, price-consciousness and decency were proven to be significantly different. 5) The consumers who were high in fashion innovativeness and fashion opinion leadership had a larger number of fashionable clothing items and experienced fashionable activities more. And there were significant differences among subdivided groups by fashion leadership in the actual state of clothing shopping.

다문화수업의 상호문화이해 유형 분석

조윤정(Cho, Youn Jung) 글로벌교육연구학회 2013 글로벌교육연구 Vol.5 No.1

본 연구는 상호문화이해를 위한 방법적 차원에서 상호문화교육의 이론을 연구하고, 그 연구를 근거하여 현재 이루어지고 있는 다문화수업 속에 상호문화교육의 원리가 어느 정도 반영되어 있는지 분석하였다. 분석 자료는 다문화정책 연구학교의 공개 수업지도안을 대상으로 선정하였고, 상호문화이해능력을 키울 수 있는 학습요소에 의한 분석과 자문화와 타문화의 비교거리를 기준으로 분석이 이루어졌다. 분석결과, 상호문화교육의 원리가 다문화수업 속에 반영되어 있지만 단순히 타문화를 기술하는 낮은 수준의 이해에 머물러 있었다. 이는 앞으로 상호문화능력을 키울 수 있도록 상호문화교육의 원리 및 방법을 우리의 다문화 상황에 맞게 정립시켜야 함을 시사한다. This study focuses on the theory that intercultural education is a method of intercultural understanding. Based on the findings, it is assumed that there is no practical intercultural education in the actual school field where its presence has been analyzed. Based on the result of the analysis, the principles of intercultural education are reflected on the multicultural instruction, however, it still remains at a low level of understanding. The characteristics of other cultures are simplified. In order to develop intercultural competency systematically, it implies that the multicultural instruction has to establish the principles and methods of intercultural education according to our multicultural situation. Therefore, practicians of multicultural education are advised to focus on applying the principles of intercultural understanding to the existing multicultural education. Also, approach methods needs to be systematized. By doing so, intercultural understanding can be realized within the school field.

패턴북 활용 초등영어 소집단 협동 쓰기 지도에 대한 연구

조윤정 ( Cho Yun-jeong ),김혜리 ( Kim Hae-ri ) 글로벌영어교육학회(구 호남영어교육학회) 2020 Studies in English education Vol.25 No.2

This study used three different types of patterned texts such as simple, dialogic, and cumulative repetitive patterns to investigate the effects on small group based cooperative writing. Twenty-two third graders participated in the study and data were collected from diverse sources for both qualitative and quantitative analysis. Two research questions were established: 1) What are the effects of using pattern books for writing instruction to early English learners? 2) How does small group based cooperative writing using pattern books impact primary English learners? The data analysis revealed the following results. First, the students’ overall writing ability was improved and their interests, confidence, and learning attitudes toward English learning were positively changed. Second, the participating children could activate English writing by writing cooperatively with their friends in small groups. During writing activities, the difference between individual and cooperative writing was observed as participants favored small group based cooperative writing over individual writing. Finally, children could build a better cooperative culture by contributing to their group work with their ideas and writing abilities. Based on the results, some suggestions are provided for future studies.