아주까리(Ricinus communis L.) 유전자원의 종실특성과 지방함량 및 지방산조성 변이

이윤호,박경호,박향민,남상영,김인재,정헌상,장영석,김홍식 한국국제농업개발학회 2010 韓國國際農業開發學會誌 Vol.22 No.3

한국의 농업유전자원센터로부터 분양받은 40점과 국내에서 수집되어진 28점을 포함한 총 68점의 아주까리 유전자원에 대한 종실특성과 지방함량 및 지방산 조성을 구명하고, 그 상호관계를 파악하여 육종 및 재배기술의 기초자료로 이용고자 수행하였으며 요약한 결과는 다음과 같다. 1. 종실 100립중의 평균은 26.6 g이었으며, 그 범위는 최소 20.1 g부터 최대 37.1 g이었다. 종실 1 l중의 평균은 481.1 g이었으며, 범위는 407.2~531 g이었다. 2. 종실길이의 평균은 12.3 mm이었으며, 범위는 10.1~14.9mm이었고, 종실넓이는 평균 5.7 mm이었으며, 범위는 4.8~6.4mm이었다. 3. 유전자원의 지방함량의 평균은 47.8%이었으며, 그 범위는 최소 41.6%에서 최대 57.2%이었고, 지방함량이 50%이상인 자원은 6종으로서 IT032142, IT104010, IT104993, IT162783, CCB056 및 CCB057이었다. 4. 포화지방산인 palmitic acid(16:0)와 stearic acid(18:0)의 함량의 범위는 각각 0.9~1.8%와 1.0~2.1%이었다. 불포화지방산인 oleic acid(18:1)의 함량의 범위는 3.6~6.5%이었고, linoleic(18:2) 및 linolenic acid(18:3) 함량의 범위는 각각 4.7~7.3% 및 0.5~1.2%이었다. Ricinoleic acid(18:1-OH) 함량의 평균은 86.2%로 아주까리의 지방산에 있어서 대부분을 차지하였고, 범위는 82.5~88.7%이었다. 5. 종실의 지방함량, palmitic, stearic, oleic, linoleic 및 linolenic acid는 ricinoleic acid 및 불포화지방산과는 부의상관, 포화지방산과는 정의 상관이었다. ricinoleic acid는 포화지방산과는 부의 상관, 불포화지방산과는 정의 상관이었고, 포화지방산과 불포화지방산과는 부의 상관이었다. This study was carried out to get basic informations on utilization of castor bean for the production of biodiesel. A total of 68 genetic resources including 40 accessions obtained from the National Agrobiodiversity Center and 28 landraces collected in Korea were screened to evaluate seed characteristics, oil content, fatty acid composition and their relationships. One hundred and one liter seed weights ranged 20.1~37.1 g and 407.2~531.0 g with averages of 26.6 g and 481.1 g, respectively. Seed length and width ranged 10.1~14.9mm and 4.8~6.4mm with averages of 12.3mm and 5.7mm, respectively. Oil content ranged 41.6~57.2% with an average of 47.8%. Six accessions, IT032142, IT104010, IT104993, IT62783, CCB056 and CCB057, showed higher oil content than 50.0%. Palmitic and stearic acid contents, saturated fatty acid, ranged 0.9~1.8% and 1.0~2.1%, respectively. Oleic, linoleic, linolenic and ricinoleic acid contents, unsaturated fatty acid, ranged 3.6~6.5%, 4.7~7.3%, 0.5~1.2% and 82.5~88.7%, respectively. Oil, palmitic, stearic, oleic, linoleic and linolenic acid contents showed negative correlation with ricinoleic acid and unsaturated fatty acid contents, and showed positive correlation with saturated fatty acid content. Ricinoleic acid content showed negative correlation with saturated fatty acid content and positive correlation with unsaturated fatty acid content. Saturated fatty acid content showed negative correlation with unsaturated fatty acid content.

골수 유래 기질 줄기세포의 탐식작용 매개성 케모카인 수용체 발현 연구

정영신,변향민,신지영,김정목,정형민,오유경 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.4

To design gene delivery systems which can deliver higher amounts of genes into stem cells, we studied the expression of receptors involved in the receptor-mediated endocytosis of bone marrow stromal stem cells. Bone marrow was isolated from ICR mice, and bone marrow stromal stem cells were isolated based on their plastic adherence property. Several cultrure conditions were screened for effective and continuous culture of marrow stromal stem cells. MesenCult medium was finally used to cultivate marrow stromal stem cells in vitro. As candidate receptors, various chemokine receptors were studied. Both bone marrow cells and marrow-derived stromal stem cells showed expression of CC chemokine receptors (CCR) and CXC chemokine receptors (CXCR). Marrow stromal stem cells showed higher expression of CCR5 and CXCR4 chemokine receptors as compared to other types of chemokine receptors. Moreover, though the expression chemokine receptors generally decreased in most chemokine receptors with the cultivation of marrow stromal stem cells, CCR5 and CXCR4 chemokine receptors retained the higher lever of receptor expressions over prolonged periods. These results suggest that the ligands exhibiting specific binding to CCR5 or CXCR4 might be used to modify gene delivery systems for increased level of receptor-mediated gene delivery into stromal stem cells.

콩 가공적성 관련성분의 종실, 두유 및 두부에서의 품종간변이 및 상호관계

박향민,송항림,박경호,정헌상,황영현,김홍식 한국육종학회 2010 한국육종학회 학술발표회 발표요지 Vol.42 No.1

Ser1778 of 53BP1 Plays a Role in DNA Double-strand Break Repairs

이정희,정향민,강미영,김상영,강윤성 대한약리학회 2009 The Korean Journal of Physiology & Pharmacology Vol.13 No.5

53BP1 is an important genome stability regulator, which protects cells against double-strand breaks. Following DNA damage, 53BP1 is rapidly recruited to sites of DNA breakage, along with other DNA damage response proteins, including γ-H2AX, MDC1, and BRCA1. The recruitment of 53BP1 requires a tandem Tudor fold which associates with methylated histones H3 and H4. It has already been determined that the majority of DNA damage response proteins are phosphorylated by ATM and/or ATR after DNA damage, and then recruited to the break sites. 53BP1 is also phosphorylated at several sites, like other proteins after DNA damage, but this phosphorylation is not critically relevant to recruitment or repair processes. In this study, we evaluated the functions of phosphor-53BP1 and the role of the BRCT domain of 53BP1 in DNA repair. From our data, we were able to detect differences in the phosphorylation patterns in Ser25 and Ser1778 of 53BP1 after neocarzinostatin-induced DNA damage. Furthermore, the foci formation patterns in both phosphorylation sites of 53BP1 also evidenced sizeable differences following DNA damage. From our results, we concluded that each phosphoryaltion site of 53BP1 performs different roles, and Ser1778 is more important than Ser25 in the process of DNA repair.

콩 종피색이 따른 항산화 활성 및 phytic acid 함량

유영은,박향민,장은규,이효재,이지석,정헌상,김홍식 한국육종학회 2011 한국육종학회 학술발표회 발표요지 Vol.43 No.-

1913년부터 2006년까지 육성된 한국 콩 품종의 아이소플라본 함량의 변이

박경호,박향민,장은규,유영은,황태영,김선림,정재현,신현만,김홍식 한국육종학회 2012 한국육종학회지 Vol.44 No.2

1913년부터 2006년까지 국내에서 육성된 콩 106 품종의 종실 isoflavone 함량의 변이를 구명하였다. 총 isoflavone의 함량은 평균 1489.0 μg/g이었고, 527.9~3436.5 μg/g의 범위이었다. 구성성분의 함량은 daidzein은 평균 650.5 μg/g이었고, 153.0~1710.4 μg/g의 범위이었으며, glycitein은 평균 146.2 μg/g이었고, 32.2~308.2 μg/g의 범위이었다. genistein은 평균 692.3 μg/g이었고, 203.4~1502.4 μg/g의 범위이었다. 총 isoflavone 함량이 높은 품종은 백천(2553.9 μg/g), 보석콩(3436.5 μg/g), 대황콩(2709.2 μg/g), 대망콩2호(2595.0 μg/g), 장기콩(2769.9 μg/g), 풍원콩(2872.8 μg/g), 소진콩(2521.8 μg/g) 및 소록콩(2956.1 μg/g)이었으며, 국내 콩 품종 중에서 보석콩이 가장 높았다. 국내에서 육성된 콩 품종들의 isoflavone 함량은 육성연대, 용도, 종실크기 및 육성모지에 따라서 유의적인 차이가 인정되었다. 육성연도에 따른 총 isoflavone 함량은 2000년대에 육성된 품종이 가장 높았고, 다음으로 1980년대와 1990년대에 육성된 품종이 높았으며, 1980년 이전에 육성된 품종이 가장 낮았다. 용도에 따른 총 isoflavone 함량은 나물용 품종이 가장 높았고, 다음으로 장류용 품종이, 그 다음으로 밥밑용 품종이 높았으며, 풋콩 및 올콩용 품종이 가장 낮았다. 종실크기에 따른 총 isoflavone 함량은 소립종이 가장 높았고, 다음으로 중립종이 높았으며, 대립종이 가장 낮았다. 육성모지에 따른 총 isoflavone 함량은 익산이 가장 높았고, 다음으로 밀양이 높았으며, 수원이 가장 낮았다. This study was carried out to evaluate the variation of isoflavone contents in 106 soybean cultivars released from 1913 to 2006 in Korea. A total of seed isoflavone content including genistein, daidzein and glycitein contents ranged from 527.9 μg/g to 3436.5 μg/g with an average of 1489.0 μg/g. The daidzein content ranged from 153.0 μg/g to 1710.4 μg/g with an average of 650.5 μg/g and the glycitein content ranged from 32.2 μg/g to 308.2 μg/g with an average of 146.2 μg/g. The genistein content ranged from 203.4 μg/g to 1502.4 μg/g with an average of 692.3 μg/g. Soybean cultivars which showed higher total of isoflavone content than any other cultivars were ‘Baegcheon’ (2553.9 μg/g), ‘Bosuk’ (3436.5 μg/g) ‘Daehwang’ (2709.2 μg/g), ‘Daemang 2’ (2595.0 μg/g), ‘Jangki’ (2769.9 μg/g), ‘Pungwon’ (2872.8 μg/g), ‘Sojin’ (2521.8 μg/g), and ‘Sorog’ (2956.1 μg/g). ‘Bosuk’ showed the highest total of isoflavone content among Korean soybean cultivars. Significant differences in isoflavone contents appeared to be related with the released years, edible use types, seed sizes and breeding locations in Korean soybean cultivars. Cultivars released in 2000s showed the highest total of isoflavone content, followed by both 1980s and 1990s, and before 1980 showed the lowest content. Cultivars for bean sprouts showed the highest total of isoflavone content, for soy sauce and paste showed the second highest content, followed by cooking with rice, and vegetable and early maturity showed the lowest content according to edible use types. Small seed cultivars showed the highest total of isoflavone content, followed by medium seed, and large seed showed the lowest content. Among three breeding locations, Iksan showed the highest total of isoflavone content, followed by Milyang, and Suwon showed the lowest content.

Williams 증후군 환아와 그 가계에서의 Fluorescent in Situ Hybridization을 이용한 Elastin 유전자 결손의 검출 및 임상 양상에 대한 평가

윤용수,정향민,김호성,노정일,최정연 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.4

Williams 증후군 환아와 그 가계에서의 Fluorescent in Situ Hybridization을 이용한 Elastin 유전자 결손의 검출 및 임상 양상에 대한 평가

김호성,정향민,허준,김연우,강윤성,이광호,노정일,최정연,윤용수 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4

Molecular Genetic Characterization of a Korean Split Hand/Split Foot Malformation (SHFM)

이광호,강윤성,정향민,문영준,이일범,김선미,김화수,Soo Youn Jun,정성국,김진숙,최지혜,조행우,손진숙,민나영 한국분자세포생물학회 2004 Molecules and cells Vol.17 No.3

국내 Fabry disease 환자의 a-Galatosidase A 유전자 돌연변이 검색

박기범,최지혜,강윤성,김선미,정향민,문영준,이광호 中央大學校 基礎科學硏究所 2001 基礎科學硏究所 論文集 Vol.15 No.-

Fabry disease(FD) is an X-linked recessive lysosomal disorder caused by a deficiency of a-galactosidase A(a-Gal A), localized at Xq22. Besides onset of pain and paresthesias in the extremities, FD was diagnosed by absence of a-Gal A activity. In this experiment the a-Gal A activity of Korean FD patients was spectrometrically analysed using an artificial substate, 4-Mrthylumbellifery1-a-D-galactoside. As expected, no a-Gal A activity was detected in lymphocytes and lymphoblastoid cells from FD patients. To screen the mutation in their a-Gal A genes, we performed single strand conformation polymorphism(SSCP) and PCR-direct sequencing form seven a-Gal A exons. The nonsense mutation was identified both in classically affected hemyzygotes and a heterozygote. They showed the C to T transition at nucleotide number 11,002, resulting in a arginine-to-stop(R342X). This result will be applicable for pre- and neonatal detection of FD and to define the genotype/phenotype correlation.