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고추 유전자원의 카로티노이드 조성 및 ASTA color 지수 분석
윤장언(Jang-Eon Yoon),김정봉(Jung-Bong Kim),김헌웅(Heon-Woong Kim),장길수(Kil-Soo Jang),고호철(Ho-Cheol Ko),장환희Hwan-Hee Jang,이성현Sung-hyen Lee,황유진(Yu-Jin Hwang) 한국육종학회 2015 한국육종학회지 Vol.47 No.3
Peppers (Capsicum annum L.) were harvested from 523 different genotypes cultivated at the Pepper Experiment Station in Gyeongsangbuk-do Agricultural Technology Administration of Yeongyang in the northeast region of Korea. The investigation of pepper with high carotenoids contents was quantitatively and qualitatively analysed by HPLC. The individual carotenoids were separated as capsanthin, lutein, zeaxanthin, β-cryptoxanthin, α-carotene and β-carotene by reverse-phase HPLC and identified with their external standards. The levels of total carotenoids and ASTA color values in 523 peppers were 0.5-84.8mg/100g of dry weight (DW) and 0.7-233.1, respectively. Individual carotenoid content and percentage of total carotenoid content were shown as follows: β-carotene (24.8%) (average 5.0 mg/100g DW) 〉 capsanthin (24.7%) (average 5.0 mg/100g DW) 〉 zeaxanthin (20.4%) (average 4.1 mg/100g DW) 〉 β-cryptoxanthin (14.8%) (average 3.0 mg/100g DW) 〉 lutein (13.3%) (average 2.7 mg/100g DW) 〉 α-carotene (2.0%) (average 0.4 mg/100g DW). As a result of plotting by using SIMCA, ASTA color values showed that the cluster is created.
스프링프레임워크를 이용한 신뢰성 평가 커뮤니티 웹 사이트의 설계 및 구현
김환희(Hwanhee Kim),장재준(Jaejun jang),이성복(Songbokg Lee),신경섭(Kyungsub Sin),박은주(Eunju Park),임한규(Hankyu Lim) 한국정보기술학회 2016 Proceedings of KIIT Conference Vol.2016 No.6
현대인들은 스마트폰의 보급과 빠른 인터넷으로 인해 수많은 정보들을 손쉽게 구하고 얻을 수 있다. 하지만 제공되는 정보들이 모두 신뢰성을 가진 것은 아니다. 페이스북이나 인스타그램 등은 ‘좋아요’를 통해 게시글에 대한 관심도의 표현은 가능하지만, 글의 신뢰도를 표현하지는 못한다. 본 논문에서는 게시글의 신뢰도를 표현하기 위해 웹 사이트의 게시글을 평가하는 방법을 제안하였다. 게시글의 평가를 통해 작성자의 신뢰성 평가가 가능하고, 이를 통해 보다 신뢰성 높은 정보의 공유가 가능한 커뮤니티 웹 사이트를 설계하고 구현하였다. In the modern world, people can easily obtain a lot of information due to supply of smartphone and fast internet. But not all information is reliable. By clicking ‘Like’ button in Facebook or Instagram, it is possible to express the interest about the post but it cannot express reliability. This paper suggests the method to evaluate post in web-site which can show its reliability. By evaluating the post, it is possible to evaluate reliability of the writer, and through this, we have designed and developed a community web-site which can share information with high reliability.
자색고구마 신자미로부터 분리한 안토시아닌 분획물의 항산화 활성과 산화스트레스에 대한 간세포 보호 효과
장환희,남송이,김미주,김정봉,김행란,이영민,Jang, Hwan Hee,Nam, Song Yee,Kim, Mi Ju,Kim, Jung Bong,Kim, Haeng Ran,Lee, Young Min 한국식품영양학회 2014 韓國食品營養學會誌 Vol.27 No.6
Anthocyanins, a class of flavonoids, are natural water-soluble pigments, which are mainly found in vegetables and fruits. Anthocyanins have attractive pharmacological activities, such as anti-oxidant, anti-inflammatory, anti-cancer, and anti-diabetic. The purpose of this study was to investigate the protective effects of anthocyanins-rich fraction (ANF) from Korean purple sweet potato variety, "Shinjami", against hydrogen peroxide ($H_2O_2$)-induced oxidative stress. In our results, pre-treatment of HepG2 cells with ANF ($10{\mu}g/mL$) significantly prevented cell death and maintained cell integrity, following exposure to 0.9 mM hydrogen peroxide. The $H_2O_2$-dependent production of intracellular ROS was also significantly decreased by pre-treatment with ANF (6 h, $10{\mu}g/mL$). In addition, ANF increased the mRNA levels of antioxidant enzymes, catalase and glutathione level in $H_2O_2$-treated HepG2 cells. These results indicated that ANF protected HepG2 cells against $H_2O_2$-induced oxidative stress by inducing protective system.
고지방식이로 유도한 지방간 마우스에서 기장 첨가식이가 지방간 및 인슐린 저항성에 미치는 영향
박미영(Mi-Young Park),장환희(Hwan-Hee Jang),이진영(Jin-Young Lee),이영민(Young-Min Lee),김재현(Jae-Hyun Kim),박재학(Jae-Hak Park),박동식(Dong-Sik Park) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.4
비알코올성 지방간은 인슐린저항성을 근간으로 하는 대사증후군의 원인으로 생각되고 있으며 최근 그 발병율이 증가하고 있다. 본 연구진은 기장 열수추출물을 식이에 첨가하여 고지방식에 의해 인위적으로 지방간을 유도한 마우스에게 섭취시킨 후 혈청 내 생물학적 수치와 간조직 검사를 통하여 비알코올성 지방간 억제 효과를 검토하였다. 그 결과, 식이섭취량은 차이가 없었으나 간조직 무게가 1% 및 2% 기장 열수추출물 첨가식이군에서 유의적으로 감소하였고(p<0.05) 간조직 내 지방 축적이 유의적으로 감소하였음을 확인하였다. 또한 기장 열수추출물 첨가식이군의 경우 고지방식 대조군에 비해 혈청 중성지방 및 총 콜레스테롤이 감소하였고(p<0.05), HDL과 HDL-/총 콜레스테롤의 비율이 유의적으로 증가하여(p<0.05) 혈액의 지질 조성이 개선되었음을 알 수 있었다. HOMA-IR 및 포도당 내성 검사 결과 2% 기장 열수추출물 첨가군의 경우 이들 모두 유의적으로 감소하여 고지방식에 의한 인슐린 저항성 및 당흡수 부전을 기장열수추출물이 완화시켰다(p<0.05). 한편 간조직에서 지방산 대사와 관련된 인자들의 유전자 발현을 측정한 결과 지방산 합성에 관여하는 L-FABP와 SCD1은 2% 기장 열수추출물 섭취군에서 유의적으로 감소하였고(p<0.05) 지방산 산화와 관련된 PPARα는 1% 및 2% 기장 열수추출물 섭취군에서 모두 유의적으로 증가하였다(p<0.05). 이상의 혈청 및 조직의 생물학적 수치와 간조직 검사 결과를 미루어 볼 때 기장 열수추출물 첨가 식이는 고지방식이에 의해 유도된 마우스의 비알코올성 지방간 치유 혹은 예방에 긍정적으로 기여할 수 있음을 시사해 준다. The dietary intake of whole grains is known to reduce the incidence of chronic diseases such as obesity, diabetes, cardiovascular disease, and cancer. In our previous study, hog millet (HM, Panicum miliaceum L.) water extract showed the highest anti-lipogenic activity among nine cereal types in 3T3-L1 cells. In this study, the effect of hog millet water extract on hepatic steatosis and lipid metabolism in mice fed a high fat diet was investigated. Mice were fed a normal-fat diet (ND), high-fat diet (HFD) or HFD containing 1% or 2% (w/w) HM for 7 weeks. Body weight and food intake were monitored during the study period. Insulin resistance by homeostasis model assessment (HOMA-IR), fasting lipid profile, hepatic fatty acid metabolism-related gene expression determined, and intraperitoneal glucose tolerance test (IGTT) were performed at the study"s end. The results indicated that 1% and 2% HM diets effectively decreased liver weights, blood TG and T-cholesterol levels (p<0.05), while the HDL-cholesterol level was increased (p<0.05) compared to HFD-induced steatotsis mice. Hepatic lipogenic-related gene (PPARα, L-FABP, and SCD1) expressions decreased, whereas lipolysis-related gene (CPT1) expression increased in animals fed the 2% PME diet (p<0.05). In addition, mice fed 1% or 2% HM diet had markedly decreased IGTT and HOMA-IR, compared to the those of the HFD-induced hepatic steatosis control group (p<0.05). These results indicated that HM inhibited hepatic lipid accumulation by regulating fatty acid metabolism, and suggested that HM is useful in the chemoprevention or treatment of high fat-induced hepatic steatosis and hepatic steatosis-related disorders including hyperlipidemia, glucose sensitivity, and insulin resistance.
알코올을 처리한 HepG2 세포에서 참죽나무 잎 추출물의 세포 보호 및 항산화 효과
김현정,조수연,김정봉,김헌웅,최정숙,장환희,Kim, Hyun-Jeong,Cho, Su-Yeon,Kim, Jung-Bong,Kim, Heon-Woong,Choe, Jeong-Sook,Jang, Hwan-Hee 한국식품영양학회 2018 韓國食品營養學會誌 Vol.31 No.4
All the parts of the Cedrela sinensis A. Juss., including the seeds, roots, and leaves, have been known to exert medicinal effects. The C. sinensis and its major compound, quercetin, were previously reported to exhibit the anti-inflammatory and anti-oxidative activities. However, the hepatoprotective effects of the C. sinensis leaves against the alcohol-induced oxidative stress in the HepG2 cells have not been studied. In this study, we investigated the antioxidant activities and analyzed the flavonoid contents of the C. sinensis-leaf extract (CE). The total flavonoid contents of the CE is 1,874.5 mg/100 g dry weight (DW), while the total quercetin 3-O-rhamnoside (quercitrin) contents, which was identified as the major flavonol in the CE, is 1,456.0 mg/100 g DW. In the ethanol-stimulated HepG2 cells, the CE effectively prevented the cytotoxic effect and increased the gene expression of the antioxidant enzymes, such as the heme oxygenase-1 (HO-1) and the glutathion peroxide (GPx). The level of the reactive oxygen species (ROS) production was significantly decreased in the CE-treated HepG2 cells. In conclusion, the C. sinensis extract suppressed the alcohol-induced oxidative stress in the HepG2 cells via the induced GPx and HO-1 gene expressions. It is expected the CE positive effects will likely be attributed to the flavonoids, like the quercetin, within the CE.
폐암세포에서 24-Methylenecyloartanyl에 반응하는 단백질 Profile
조일규 ( Lkyu Cho ),( Sofia Doello ),장환희 ( Hwan Hee Jang ),김정봉 ( Jung Bong Kim ),( Qing X. Li ) 한국환경농학회 2017 한국환경농학회 학술대회집 Vol.2017 No.-
The protein profiles were determined in response to 24-mCAF (24-methylenecycloartanyl ferulate) to show activity of anti-proliferation in the 549 cell lines and understand the responded protein networks in the cell lines after 72 h of incubation with 24-mCAF. The under-represented, over-represented and overlapped proteins identifies (IDs) were mapped into biological networks and pathways of interacting protein clusters according to the identifiers` HomoloGene to the ortholog information in the Ingenuity Knowledge Base (IKB) (Homo sapiens/humans data) using the Ingenuity Pathway Analysis (IPA). Twenty five networks and 42 pathways were up-regulated in the lungs of 50 μM 24-mCAF-treated A549 cells, whereas 25 networks and 54 pathways were down-regulated. The top down-regulated tox lists included VDR/ RXR activation, G2/M DNA damage checkpoint regulation, PPARα/RXRα activation and p53 signaling. Top up-regulated functions in the 50 μM 24-mCAF-treated A549 cells were cardiovascularcancer- respiratory, which is related to respiratory-nitric oxide synthase, clathrin-mediated endocytosis, which is regulated by AP2-associated protein kinase, immune response/oxidative stress/pulmonary hypertension, non-small cell lung cancer, which comprises about 80% of all lung cancers, neurotransmitter/collagen synthesis. Top down-regulated functions were epithelial cell differentiation signaling, cartilage/cerellum/lung development, mitogen-activated protein kinases signaling, transforming growth factor ß (TGF- ß) signaling, and tumor suppressor.