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세신추출물이 α-MSH 자극에 의한 B16F10 세포의 멜라닌생성에 미치는 영향
Ji Yeon Jang(장지연),Ha Neui Kim(김하늬),Yu Ri Kim(김유리),Byung Woo Kim(김병우),Yung Hyun Choi(최영현),Byung Tae Choi(최병태) 한국생명과학회 2010 생명과학회지 Vol.20 No.11
α-MSH는 세포내 cAMP를 증폭시켜 멜라닌세포의 증식과 색소 증가에 관여한다. 본 연구에서는 α-MSH로 자극한 B16F10 세포에서 세신추출물의 hypopigmenting 효과를 조사하고 그 억제기전에 대하여 조사하였다. 세신 추출물은 α-MSH에 의해 유도된 tyrosinase 활성과 멜라닌생성을 효과적으로 억제시켰으며, 이는 tyrosinase 발현을 조절하는 전사인자인 MITF의 발현억제와 연관성이 있었다. 즉 세신추출물은 MEK/ERK와 PI3K/Akt의 활성화를 통하여 MITF를 조절함으로서 α-MSH에 의해 유도되는 tyrosinase, TRP-1, Dct 등 멜라닌생성관련 단백질을 억제함으로서 멜라닌생성을 저해하는 것으로 사료된다. Recently, it has been found that Asiasari radix showed a hypopigmenting effect on melanogenesis through activation of mitogen-activated protein kinase (MEK)/extracellular signal-activated kinase (ERK) in B16F10 melanoma cells. However, the hypopigmenting effect of A. radix on the α-melanocyte stimulating hormone (α-MSH)-stimulated melanogenesis has remained unknown. The purpose of this study was to investigate the inhibitory mechanism of the partially purified A. radix (PPAR)-induced hypopigmentating effects on α-MSH-stimulated melanogenesis in B16F10 mouse melanoma cells. PPAR strongly inhibited tyrosinase activity and leads to decreased melanin synthesis in α-MSH-stimulated B16F10 melanoma cells. PPAR also decreased the α-MSH-induced over-expression of the melanogenic enzymes, tyrosinase, tyrosinase-related protein (TRP)-1, dopachrome tautomerase (Dct) and microphthalmia-associated transcription factor (MITF). We further showed that PPAR inhibits α-MSH-induced melanogenesis via phosphorylation of MEK/ERK and PI3K/Akt, and that their activation was blocked by MEK inhibitors, PD98059 and PI3K inhibitors, LY294002 in α-MSH-stimulated B16F10 melanoma cells. These results suggest that PPAR inhibits α-MSH-induced melanogenesis by activation of MEK/ERK and PI3K/Akt through MITF degradation, which may lead to down-regulation of tyrosinase.
김현주(Kim, Hyun Ju),장지연(Jang, Ji Yeon) 한국디자인문화학회 2017 한국디자인문화학회지 Vol.23 No.1
미용 서비스산업은 양적팽창으로 인해 경쟁이 점점 심화되면서 경쟁우위를 확보하기 위해 고객서비스에 주목하고 있다. 이를 위해 고객과의 관계를 지속적으로 유지하기 위한 관계자산에 대한 이해를 높일 필요성이 있을 것이다. 이에 본 연구에서는 헤어미용실의 충성도, 특별인식⋅우대, 친밀감, 지식형성 프로그램의 관계자산이 고객시민행동에 어떠한 영향을 미치는가를 분석하고, 그 결과를 바탕으로 고객과의 관계를 장기적으로 유지하기 위해 우선적으로 관리⋅개선해야하는 서비스 요인을 파악하여 새로운 경영 전략 수립을 위한 정보를 제공하고자 한다. 본 연구의 설문은 광주⋅전남에 위치한 헤어미용실을 이용하는 고객을 대상으로 2016년 12월 10일부터 2017년 1월 10일까지 500부를 배포하여 분석에 적합하지 않은 설문지를 제외한 470부를 최종 분석에 사용하였다. 자료 분석은 SPSS v. 21.0 프로그램을 활용하여 빈도분석, 요인분석, 신뢰도 검증을 위한 Cronbach"s a 계수 산출, 상관관계분석, 다중회귀분석을 실시하였다. 연구결과, 헤어미용실의 관계자산 하위요인 중 친밀감, 지식형성, 특별인식⋅우대, 충성도 프로그램은 충성행동에 영향을 미치는 것으로 나타났다. 또한 관계자산의 하위요인 중 지식형성, 친밀감 프로그램은 협력행동에 영향을 주는 것으로 확인되었으며, 참여행동에는 지식형성, 특별인식⋅우대 프로그램이 영향을 미치는 것으로 나타났다. 따라서 관계자산 중 지식형성 프로그램은 고객시민행동에 공통적으로 영향을 주는 중요한 요인으로 도출되어 미용 경영자는 체계적인 직원교육을 위한 시스템 구축을 통해 기본매너, 문제해결 교육 등을 중심으로 관리⋅개선하여 직원에 대한 신뢰를 강화시키기 위한 노력이 필요할 것이다. Competition in the hair salon industry has become fiercer as it expands. Under these circumstances, businesses have paid attention to customer services to stay competitive. For this, there is the need to have a better understanding of relationship assets in maintaining a good customer relationship. Therefore, this study analyzed the effects of the loyalty of hair salons, special recognition and preference, intimacy, and relationship assets to knowledge formation of customer citizenship behavior and examined the service factors that should be managed and improved in advance to maintain good customer relationships over a long period of time with a goal of providing the data needed to develop new management strategies. In terms of the research method, 500 questionnaires were distributed to the customers of the beauty salons in Gwangju and Jeonnam from December 10, 2016 to January 10, 2017. Among them, 470 copies excluding inappropriate ones were used for final analysis. For data analysis, the following were performed using SPSS v. 21.0: frequency analysis, factor analysis, calculation of Cronbach"s alpha for reliability testing, correlation analysis and multiple regression analysis. The results found that among the subordinate factors of hair salons relationship assets, intimacy, knowledge formation, special recognition and preference, and loyalty influenced loyalty behavior. Among the sub-factors of relationship assets, in addition, intimacy formation and intimacy affected cooperation behavior. In participation behavior, knowledge formation and special recognition and preference were influential. In sum, it was shown that among relationship assets, knowledge formation commonly influenced customer citizenship behavior. Therefore, hair salon owners must work to strengthen customer trust of employees through employee management and improvement through basic manners and problem-solving education after establishing a systematic employee training and education system.
증례 : 신장; 말기신부전 환자에서 Candida famata에 의해 발생한 심내막염
김정관 ( Jeong Gwan Kim ),황현철 ( Hyun Chul Whang ),장지연 ( Ji Yeon Jang ),하성은 ( Seong Eun Ha ),김동휘 ( Dong Hwi Kim ),최범순 ( Bum Soon Choi ) 대한내과학회 2014 대한내과학회지 Vol.86 No.3
Infective endocarditis is rare in end-stage renal disease (ESRD) patients, who have a poorer prognosis than the general population. Candida endocarditis is rare and has a poor prognosis among causes of infective endocarditis. A 45-year-old male was admitted with sepsis combined with a hematoma on his right back. Candida famata was cultured in his blood. We treated him with antifungal agents. Echocardiography was performed to identify vegetations and diagnose endocarditis. In this case, surgical therapy was impossible because the patient`s condition had deteriorated. We also administered antibiotics because methicillin-resistant Staphylococcus aureus was cultured from his sputum and a perianal abscess. The symptoms did not improve despite the ongoing treatment. Metabolic acidosis, hypotension, and a decreased state of consciousness developed and he died. We report a rare case of Candida endocarditis in an ESRD patient on hemodialysis.