특집 제2차 과학기술기본계획-(3) 기초연구 진흥

이혜숙,Lee, Hye-Suk 한국과학기술단체총연합회 2008 과학과 기술 Vol.41 No.3

큰 도전과 대단한 기회들

이혜숙,Lee, Hye-Suk 한국과학기술단체총연합회 2006 과학과 기술 Vol.39 No.4

과기정론 - 첨단 연구장비 전문인력 육성 시급하다

이혜숙,Lee, Hye-Suk 한국과학기술단체총연합회 2012 과학과 기술 Vol.513 No.-

소박한 겉장을 열면 조촐한 생애가

이혜숙,Lee, Hye-Suk 대한출판문화협회 1996 출판저널 Vol.199 No.-

Synthesis and Evaluation of Coumaroyl Dipeptide Amide as Potential Whitening Agents

이혜숙,신경훈,Geun-Seog Ryu,조인식,김재일,Jae-Ho Lee,김한영 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.10

Coumaroyl dipeptide amide, Coumaric acid-LG-NH2, was prepared successfully using the solid-phase method, and its efficacy as a skin whitening agent was studied. Coumaric acid-LG-NH2 was prepared with Rink-amide resin, and 96.354% of purity was obtained. Using MTT assay and LDH release assay, we found that it exhibited very low cytotoxicity. And, we found that Coumaric acid-LG-NH2 inhibited tyrosinase activity dose-dependently and showed superior tyrosinase inhibitory activity to well-known whitening agent, arbutin. IC50 value of Coumaric acid-LG-NH2 was 182.4 μM, and IC50 value of arbutin was 384.6 μM. Also, in measurement of melanin contents using B16F1 melanoma cell lines, Coumaric acid-LG-NH2 reduced melanin production induced by α-MSH statistically significant, and showed superior melanin inhibitory activity to p-coumaric acid or arbutin. In addition, Coumaric acid-LG-NH2 reduced MC1R mRNA expression level. Thus, we concluded that MC1R pathway is the significant pathway of Coumaric acid-LG-NH2, and Coumaric acid-LG-NH2 has great potential to be used as novel whitening agents.

마취된 흰쥐 해마신경세포에서 Neurobiotin 이온주입으로 인한 신경세포의 생리적 특성의 변화

이혜숙,이만기,김영진,최병주,Lee, Hye-Sook,Lee, Maan-Gee-G.,Kim, Young-Jin,Choi, Byung-Ju 대한소아치과학회 1999 大韓小兒齒科學會誌 Vol.26 No.2

Pyramidal cells in the hippocampal CA area were recorded from and filled with neurobiotin in anesthetized rats. The extent of their dendrites and the electropharmacological properties of membrane as well as the effect before and after neurobiotin injection were examined. Pyramidal cells had a high resting membrane potential, a low input resistance, and a large amplitude action potential. A afterhyperpolarization was followed a single action potential. Most pyramidal cells did not display a spontaneous firing. Pyramidal cell displayed weak inward rectification and anodal break excitation in response to negative current injection into the cell. Membrane properties of recorded neurons before and after neurobiotin injection with consecutive current injection were compared. Some properties were significantly increased after labelling(P>0.05); the duration and amplitude of sustained AHP, input resistance, and the number of action potentials for simultaneous intra- and extracellular stimulations. Neurobiotin-filled neurons showed pyramidal morphology. Cells were generally bipolar dendrite processes ramifying in stratum lacunosum-moleculare, radiatum, and oriens. 마취된 흰쥐를 사용하여 해마의 CA영역에 위치한 피라밋세포들의 세포막 특성을 in vivo의 세포내 기록법에 의해서 관찰한 후 2.5% neurobiotin을 세포내 기록용 미세전극에 채워 세포내로 충진시킨후 충진전과 동일한 실험순서로 반응을 다시 관찰하고 ABC kit를 이용하여 면역조직염색을 행하여 형태학적인 관찰을 하였다. 피라밋세포의 세포내 반응 특성은 높은 휴지막 세포막전위, 낮은 input resistance 그리고 큰 활동전위를 가졌다. neurobiotin 충진 전 후에 따른 세포막 특성의 변화는 sustained AHP의 duration과 amplitude, input resistance, 그리고 세포외 및 세포내 자극에 따른 AP 수에서 유의한 차이를 보였고(P<0.05), 세포외 자극에 의한 억제는 주로 전반부에 나타났으며 CA3 영역에 위치한 이 세포의 형태학적 관찰 결과 세포체는 피라밋층에서 분명한 피라미드 형태를 띄고 있었고 기저 및 선단 가지가 각각 백색판층 및 섬유방-분자층까지 뻗어 있었으며 축삭은 겉질을 향해 기저가지돌기면에서 수직으로 뻗어 있었다. 해마의 주세포인 피라밋세포의 세포막 특성과 세포내 염색지시체(marker)로 주로 쓰이는 neurobiotin에 의해 세포막 특성중 일부가 변화됨을 알 수 있었고, 뇌내의 신경세포연결망이 완전히 보존되어 세포들 사이의 시냅스관계를 추측할 수 있는 in vivo 실험 모델이 응용될 수 있음을 제시하였다.

기후변화에 따른 다목적댐 저수지의 취약성 평가

이혜숙,Lee, Hye-Suk 한국수자원학회 2012 물과 미래(한국수자원학회지) Vol.45 No.11

Safety effect of fermented oyster extract on the endocrine disruptor assay in vitro and in vivo

이혜숙,Hyun Hwang-Bo,Seon Yeong Ji,Seyeon Oh,Kyung-A Byun,Joung-Hyun Park,Bae-Jin Lee,Gi Young Kim,최영현 한국수산과학회 2021 Fisheries and Aquatic Sciences Vol.24 No.10

Oyster (Crassostrea gigas) is a marine bivalve mollusk widely distributed in coastal areas, and have been long widely used in industrial resources. Several studies demonstrated that fermented oyster (FO) extract attribute to bone health, but whether administration of FO play as an endocrine disruptor has not been studied. Therefore, in the present study, we investigated the effect of FO on the endocrine system in vitro and in vivo. As the results of the competitive estrogen receptor (ER) and androgen receptor (AR) binding affinities, FO was not combined with ER-α, ER-β, and AR. However, 17β-estradiol and testosterone, used as positive control, were interacted with ER and AR, respectively. Meanwhile, oral administration of 100 mg/kg and 200 mg/kg of FO doesn’t have any harmful effect on the body weight, androgen-dependent sex accessory organs, estrogen-dependent-sex accessory organs, kidney, and liver in immature rats. In addition, FO supplementation has no effect on the serum levels of luteinizing hormone (LH), follicle stimulating hormone (FSH), testosterone, and 17β-estradiol. However, the relative weight of androgen- and estrogen-dependent organs were significantly increased by subcutaneously injection of 4.0 mg/kg of testosterone propionate (TP) and by orally administration of 1.0 μg of 17α-ethynyl estradiol (EE) in immature male and female rats, respectively. Furthermore, TP and EE administration markedly decreased the serum LH and FSH levels, which are similar those of mature Sprague-Dawley (SD) rat. Furthermore, the testosterone and 17β-estradiol levels were significantly enhanced in TP and EE-treated immature rats. Taken together, our findings showed that FO does not interact with ER and AR, suggesting consequentially FO does not play as a ligand for ER and AR. Furthermore, oral administration of FO did not act as an endocrine disruptor including androgenic activity, estrogenic activity, and abnormal levels of sex hormone, indicating FO may ensure the safety on endocrine system to develop dietary supplement for bone health.

도서지역 주민의 홉입 알레르겐 감작률과 알레르기 질환의 연관성

이혜숙 ( Hye Sook Lee ),홍성철 ( Seong Chul Hong ),김정홍 ( Jeong Hong Kim ),김재왕 ( Jae Wang Kim ),이근화 ( Keun Hwa Lee ),이재천 ( Jae Chun Lee ),정진호 ( Jin Ho Jeong ) 대한보건협회 2013 대한보건연구 Vol.39 No.2

Objectives: This study tries to reveal the aeroallergen sensitization rates and their connection on allergic diseases. The people in this study were the inhabitants on Udo Isle, one of the small isles near Jeju Island. Methods: The survey was performed from June to July, 2012. The target was the inhabitants residing on Udo Isle and their age ranged from 5 to 69. The questionnaire survey was applied, using International Study of Asthma and Allergies in Childhood(ISAAC). Skin prick tests were also conducted, using 16 types of aeroallergens. For the questionnaire survey, the interviewers visited every family. With regard to children(5-19 years old), their parents` responses were collected. For adults, their self reports and face-to-face interviews were enrolled This analysis included 176 effective responses(79.6%) among the 221 examinees who consented the questionnaire survey and skin prick tests. Results: With regard to asthma, the prevalence of “wheeze, last 12 months” was 7.4%, 21.3% for “allergic rhinitis, last 12 months”, and 12.6% for “itchy eczema, last 12 months”. The sensitization rate of aeroallergens was 22.2%. Children`s sensitization rate(38.1%) was higher than that of adults(17.2%). The aeroallergens representing higher sensitization rate was listed as the following order: D. pteronyssinus, D. farinae, citrus red mite, and mould mixtureⅠ. This survey revealed that house dust mite such as D. pteronyssinus or D. farinae was the one of the major allergens for allergic rhinitis. Conclusion: Like the inhabitants of many other regions, the residents on the Udo Isle were highly sensitized to house dust mite, with the major connection on allergic rhinitis. Compared to the Jeju Island, the sensitization rate of Citrus red mite and Japanese cedar was very low. The further systematic study regarding time trends in the prevalence and cause of allergic disease in relation to the environmental changes, might be needed.

학령기 아동의 식품알레르기 유병률과 식품 알레르겐 감작률 조사

이혜숙 ( Hye Sook Lee ),홍성철 ( Sung Chul Hong ),김정홍 ( Jeong Hong Kim ),김재왕 ( Jae Wang Kim ),이근화 ( Keun Hwa Lee ),이재천 ( Jae Chun Lee ) 대한보건협회 2014 대한보건연구 Vol.40 No.3

연구목적 : 본 연구는 학령기 아동의 식품알레르기 유병률과 식품 알레르겐의 감작률을 조사하고자 하였다. 연구방법 : 2011년 4월부터 5월까지 제주지역 3개 초등학교 1, 2학년 1,189명을 대상으로 ISSAC 설문지를 이용한 자기기 입식 설문조사와 12종의 식품 알레르겐에 대해 피부단자시험(Skin Prick Test)을 시행하였다. 연구결과 : 학령기 아동의 “평생 식품알레르기 증상 유병률(Food Allergy, ever)”은 10.5%, “최근 식품알레르기 증상 유병률 (Food Allergy, last 12 mo)” 4.8%, “평생 식품알레르기 진단 유병률 (Diagnosis of Food Allergy, ever)”5.8%, “최근 식품알레르기 치료 유병률(Treatment of Food Allergy, last 12 mo)” 2.6%로 타 지역 소아와 비슷한 수준을 보였다. 1개 이상의 식품알레르겐에 감작률을 보인 경우는 3.7%로 남자(4.8%)가 여자(2.5%)보다 감작률이 유의하게 높았다(p=0.043). 가장 높은 감작률을 보인 식품 알레르겐은 새우(1.1%), 계란흰자(0.6%), 홍합(0.5%), 계란노른자(0.4%), 우유(0.4%) 순이었으며, 식품알레르기증상 발생에 연관이 있는 알레르겐은 계란흰자, 계란노른자, 참치, 닭고기, 대두 등이었다. 다변량 분석결과, 식품알레르기 발생과 가장 관련이 있는 원인 식품은 계란이었으며, 계란에 감작되었을 때 식품알레르기 발생 위험도가 10.53배 높은 것으로 나타났다. 결론 : 제주지역 학령기 아동의 식품알레르기 유병률은 다른 지역과 큰 차이가 없었으나, 2008년도에 비해 증가 추세를 보였다. 가장 높은 감작률을 보인 것은 계란이었으며, 학령기 아동의 식품알레르기 발생의 주요 원인 식품으로 확인되었다. 추후에도 각 지역사회 혹은 학교단위 별로 식품알레르기에 대한 정기적인 역학조사와 함께 맞춤형 교육이 필요하다. Objectives : This study is to survey school-aged children``s prevalence of food allergy and to investigate sensitization rates to common food allergens. Methods : The self-administered questionnaire survey and skin prick tests with 12 food allergens were applied to school children in Jeju, Korea. From 3 elementary schools, 1,189 children were enrolled with informed consent. Results : The lifetime prevalence of self-reported food allergy were 10.5%. The period prevalence of self-reported food allergy(last 12 months), the lifetime prevalence of clinician-diagnosed food allergy and the period prevalence of clinician-treated food allergy(last 12 months) were 4.8%, 5.8% and 2.6%, respectively. The sensitization rate to one or more food allergens was 3.7%. Male(4.8%) showed more sensitized than female (2.5%)(p=0.043). Shrimps(1.1%), egg white(0.6%), mussels(0.5%), yolk(0.4%) and milk(0.4%) were commonly sensitized food allergens. The commonly associated allergens in the lifetime prevalence of food allergy include egg white, yolk, tuna, chicken and soybeans. The multivariate analysis revealed that the allergens most significantly related with the lifetime prevalence of food allergy were eggs, showing the risk of food allergy was 10.53 times higher in the cases with sensitization to egg. Conclusion : Compared to those in other regions, the school-aged children in Jeju show no difference in the prevalence of food allergy, but, with a gradual increase in frequency. Among the food allergens, egg is one of the most commonly sensitizing allergen as well as cause of food allergy. Serial epidemiological investigations and customized educational programs concerning food allergy are necessary at school.