골반저근운동프로그램이 긴장성 요실금 여성노인의 하부요로증상과 최대질수축압, 골반저근 활성도에 미치는 효과

이해현 ( Hae Hyun Lee ),이승원 ( Seung Won Lee ),송창호 ( Chang Ho Song ) 체육과학연구원 2009 체육과학연구 Vol.20 No.3

이 연구의 목적은 골반저근운동프로그램이 긴장성 요실금 여성노인의 하부요로증상과 최대질수축압, 골반저근 활성도에 미치는 효과를 알아보고자 하는 것이다. 선정기준에 부합된 25명의 긴장성 요실금 여성노인을 무작위로 골반저근운동군과 대조군으로 각각 13명과 12명으로 나누어 골반저근운동군에게 주 2회의 골반저근운동프로그램과 주 7회의 개인 운동을 8주 동안 실시하였으며, 대조군에게는 특별한 운동 없이 일반적인 교육만 실시하였다. 골반저근운동프로그램의 효과를 파악하기 위하여 운동 전·후에 하부요로증상에 대한 설문조사, 질압측정기를 이용한 최대질수축압, 근전도를 이용한 골반저근 활성도 검사를 시행하였다. 본 연구의 자료분석은 운동 전·후 효과를 비교하기 위해 대응표본 t 검정을 실시하였고, 집단 간의 차이를 비교하기 위해 독립표본 t 검정과 x2? 검정을 실시하였다. 8주 간의 골반저근운동프로그램으로 인해 하부요로증상은 유의하게 감소하였고(p<.05), 최대질수축압과 골반저근 활성도는 유의하게 증가하였지만(p<.05), 대조군에서는 유의한 차이를 나타내지 않았다. 이상의 연구를 통하여 골반저근운동프로그램은 긴장성 요실금 여성노인의 요실금 치료에 효과적인 운동방법으로 요실금을 가진 여성노인에게 삶의 질을 향상시키는데 크게 기여할 것으로 기대된다. The purpose of the present study was to evaluate the effect of pelvic floor muscle training(PFMT) on lower urinary tract symptom, maximum vaginal contraction pressure and pelvic floor muscle activity in Aged women with urinary stress incontinence. Twenty-five aged women were divided two groups by random; experimental(PFMT) group and control group. PFMT group completed the 8-weeks PFMT program and PFMT group had a structured training program for 60 minutes twice per week and they were encouraged to PFMT at home five days for a period of 8 weeks. Control group were not participated the PFMT program and took the general education. They were measured by questionnaire of urinary symptoms, perineometry, and electromyography. At the end of the study, the lower urinary symptom, the maximum vaginal contraction pressure, and the pelvic floor muscle activity were significantly increased(p<0.05). In conclusion, PFMT program was effective for treatment urinary incontinence in aged women and is expected to improve quality of life on aged women with urinary incontinence.

TENS가 시각장애 청소년의 균형능력에 미치는 효과

이해현 ( Hae Hyun Lee ),이승원 ( Seung Won Lee ),송창호 ( Chang Ho Song ) 한국특수체육학회 2008 한국특수체육학회지 Vol.16 No.1

부하자극이 만성뇌졸중 환자의 정적 균형, 근육 활성도에 미치는 효과

이승원 ( Seung-won Lee ),신원섭 ( Won-seob Shin ),인태성 ( Tae-sung In ),이순현 ( Sun-hyun Lee ),이해현 ( Hae-hyun Lee ),이경진 ( Kyoung-jin Lee ),송창호 ( Chang-ho Song ) 대한물리치료학회 2009 대한물리치료학회지 Vol.21 No.1

Purpose: We investigated the changes that effects of load stimulation (LS) on static balance and muscle activities in normal healthy elders and chronic stroke patients. Methods: Subjects were instructed twenty normal healthy elders (8 men and 12 women) and ten chronic stroke patients (4 men and 6 women). They were tested while standing on a force platform under two conditions; LS, no LS were applied in random order. Prior to testing, adhesive surface EMG electrodes were fastened to the skin overlying the right (non-paralytic side in stroke patient) bellies of tibialis anterior (TA) and lateral gastrocnemius (LG) muscles. They were assessed on postural sway and %MVC (Maximum voluntary contraction) of TA and LG. Results: Participants showed that the application of LS brought about a decrease in postural sway as expressed by average sway path and velocity. %MVC of TA and LG were increased. The application of LS to normal and stroke patients decreases postural sway during quite stance. Conclusion: These findings the application of LS to normal and stroke patients improves static balance.

소아 알레르기 환자에서 혈청 흉선과 활동화 조절 케모카인, 대식세포 유래 케모카인의 상승과 호산구 염증지표와의 연관성

정보현 ( Bo Hyun Chung ),이해성 ( Hae Sung Lee ),서현석 ( Hyeon Seok Seo ),박하늘 ( Ha Neul Park ),지그프리드바우어 ( Siegfried Bauer ),서성철 ( Sung Chul Seo ),윤원석 ( Won Suck Yoon ),정지태 ( Ji Tae Choung ),유영 ( Young Yoo ) 대한천식알레르기학회 2012 천식 및 알레르기 Vol.32 No.4

Background: Thymus and activation-regulated chemokine (CCL17) and macrophage-derived chemokine (CCL22) are known as important mediators in allergic inflammation. Recently many researchers have focused on these mediators, but the role of the serum CCL17 and CCL22 in allergic diseases in children are still controversial. The aims of this study were to compare serum levels of CCL17 and CCL22 between children with different manifestation of allergic diseases, such as bronchial asthma, allergic rhinitis and atopic dermatitis, to analyze relationship with blood eosinophil makers and to find clinical significance of these markers. Methods: A total of 49 children (14 bronchial asthma, 13 allergic rhinitis and 13 atopic dermatitis) and the 9 healthy control subjects were enrolled in this study. Clinical characteristics and serum chemokine (CCL17, CCL22) levels were analyzed. We examined whether serum levels of CCL17 and CCL22 would be related to serum immunoglobulin E levels, blood eosinophils and serum eosinophil cationic protein concentrations. Results: Serum levels of CCL17 and CCL22 were significantly higher in children with bronchial asthma and atopic dermatitis than that in controls. Serum levels of CCL17 and CCL22 were significantly related to serum immunoglobulin E levels, blood eosinophil counts and serum eosinophil cationic protein concentrations with different strength in children with allergic diseases. Conclusion: Serum CCL17 and CCL22 may play a crucial role in the chronic allergic inflammatory process and can be used as inflammatory markers. These suggest that serum CCL17 and CCL22 might be involved in the pathophysiology of allergic diseases in children.

비다공성 막을 이용한 마취제의 경피투여 치료 시스템 (1)

권현(Hyun Kwon),강길선(Gil Son Khang),송해영(Hae Young Song),이해방(Hai Bang Lee) 한국고분자학회 1999 폴리머 Vol.23 No.3

저항강도 최적화에 기반한 케이블부재의 코드 캘리브레이션

이호현(Lee, Ho Hyun),이해성(Lee, Hae Sung) 대한토목학회 2018 대한토목학회 학술대회 Vol.2018 No.10

실험용 쥐의 마취

최희락,고종현,이해범,이준모,Choi, Hee-Rack,Ko, Jong-Hyun,Lee, Hae Beom,Lee, Jun-Mo 대한미세수술학회 2013 Archives of reconstructive microsurgery Vol.22 No.1

Rats and mice are commonly used in experimental laboratories and anesthetic drugs are important for researchers to understand the details. Administration of fluids helps to stabilize the experimental animals before anesthesia via intravenously through the lateral vein in rats and in case of difficulty in catheterization and maintenance, fluids are usually administered as boluses. Large volumes of cool fluids will rapidly lead to hypothermia and all parenteral fluids must be warmed to body temperature before administration. Premedication with a sedative may ease induction with volatile anesthetic drugs. The first choice for rodent anesthesia is complete inhalational anesthesia. The second option is using injectable anesthesia. Recovery from the volatile agents that have been used rapid when the agent is no longer administered. Anesthetic monitoring equipment is an infant-size bell sthethoscope that can be used to ausculate the heart and lungs. Supplemental heating should be provided to reduce the heat loss supply and maintain core body temperature. The kinds of drugs, characteristics, route of administration and care after surgery were reviewed and summarized from the references. Anesthetic drugs, maintenance, monitoring and aftercare are important in the laboratories to keep the animal safe in all experimental procedures.

Experion(TM) assay system을 이용한 난소암 혈청 종양표지물의 발굴

민현진 ( Hyun Jin Min ),배수미 ( Su Mi Bae ),곽선영 ( Sun Yong Kwak ),양민아 ( Min Ah Yang ),이해남 ( Hae Nam Lee ),김용욱 ( Yong Wook Kim ),신종철 ( Jong Chul Shin ),김정식 ( Jung Sick Kim ),남계현 ( Kye Hyun Nam ),김용완 ( Yong 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.5

목적: 난소암은 세계적으로 여성에게서의 발생률과 사망률이 높은 악성 종양이다. 불행히도, 아직 임상적인 예후나 특징이 두드러지게 드러나지 않는다. 사실, 높은 사망률은 임상적인 조기진단의 어려움에 있다. 본 연구에서 LabChip 테크놀로지 시스템과 자동화 전기영동 시스템인 Experion(TM)으로 난소암과 관련한 단백질 발현을 위한 연구를 하였다. 재료 및 방법: 분석은 14명의 건강한 여성의 혈청과 28개의 난소암환자의 혈청을 사용하였다. 각각의 혈청은 가톨릭의과대학 임상연구위원회 (IRBC)의 승인을 받았고 강남성모병원으로부터 제공받았다. Experion(TM) (Bio-Rad, Hercules, CA)을 사용하여 분석하였다. 그 후 SDS-PAGE 전기영동하여 Coomassie stain으로 확인한 후, MALDI-TOF-MS로 단백질 확인하였다. 결과: 난소암 관련된 단백질의 분석은 Experion(TM)의 고 처리 검색 (High throughput screening)으로 단백질 정량 값을 볼 수 있었다. 난소암에 있어 높은 민감도와 특이성으로 선별함으로써 Experion(TM) 정량방법이 입증되었다. Ceruloplasmin (115.0 kDa), hemoglobin β chain (15.9 kDa), hemoglobin δ chain (10.7 kDa), serum amyloid A4 (14.8 kDa) 그리고 amyloid relate serum protein SAA (11.7 kDa)이 난소암 환자 혈청으로부터 발견되었다. 난소암 환자와 건강한 여성 사이에서 이들 5개의 단백질의 발현이 차이를 보였다. Experion(TM) assay system은 높은 수준의 정확도를 유지했고, 처리능을 증가시켜 난소암 관련 단백질의 분석을 위해 제공되었다. 결론: 고처리 Experion(TM) assay system으로 난소암에서 새로운 단백질을 확인할 수 있었다. 또한, Experion(TM) assay system은 임상적인 적용을 위한 확실한 데이터베이스로 난소암의 진단을 위해 쉽게 적용될 수 있을 것이다. 본 연구는 Experion(TM) assay system을 이용한 난소암의 잠재적인 혈청 종양표지물의 확인을 위한 첫 번째 보고임에 의미가 있다. Objective: The discovery of new biomarkers for ovarian cancer is clearly necessary for the detection and monitoring of the disease. Experion(TM) automated electrophoresis system can be employed in the identification of differentially expressed proteins in cancer cells. The objective of this study was to discover potential diagnostic serological biomarkers for ovarian cancer. Methods: We performed protein expression difference analyses for 14 healthy women and 28 ovarian cancer patients with stage I, III and IV using Experion(TM) system. And then we checked the protein expression as silver staining after loading at 8~16% gradient gel for comparison with Experion(TM) gel image. The candidate biomarkers were purified and determined using MALDI-TOF mass spectrometer. Results: The distinctive polypeptide peaks were detected at 115.40, 15.96, 14.8, 11.66, and 10.69 kDa and these five peaks were identified as ceruloplasmin, hemoglobin β chain, hemoglobin δ chain, serum amyloid A4, and amyloid related serum protein SAA, respectively. These proteins were significantly different between the sera of normal healthy women and ovarian cancer patients. Conclusions: Five proteins were found to be significantly different between the sera of normal healthy women and ovarian cancer patients. In addition, Experion(TM) assay system can provide high performance for analysis of ovarian cancer-related proteins by increasing the throughput while maintaining a high level of accuracy.

희석혼합체의 기계적 분쇄에 의한 나노 CeO₂의 합성

임건자,김태은,이종호,이해원,이동주,현상훈,Lim, Geon-Ja,Kim, Tae-Eun,Lee, Jong-Ho,Lee, Hae-Weon,Rhee, Dong-Joo,Hyun, Sang-Hoon 한국세라믹학회 2002 한국세라믹학회지 Vol.39 No.8

The nanocrystalline $CeO_2$ was synthesized by mechanical milling and subsequent heat-treatment with the mixture of $Ce(OH)_4$ precursor and diluent, NaCl. Using deionized water, the diluent, NaCl, in the mixture has been easily dissolved out. Diffusion barrier was provided by the diluent during heat-treatment, which suppressed not only the coarsening of primary particle but also the agglormeration between the particles. Crystallite and aggregate size of $CeO_2$ depended on the concentration of diluent, temperature and time of heat-treatment; increased with the temperature and time increases. In case the mixture was heat-treated at high than $600^{\circ}C$, however, the crystallite size was saturated near 20 nm, which was supposed to be due to the densification of diluent. 산화물의 금속 전구체인 $Ce(OH)_4$를 희석제인 NaCl과 함께 기계적으로 분쇄하여 나노 크기의 일차입자를 제조하고, 분쇄한 전구체 분말은 희석제와 함께 열처리를 하여 나노 결정립의 $CeO_2$를 합성하였다. 희석제는 전구체의 분쇄시 분말의 재응집을 억제하여 분쇄효율을 증진시켰을 뿐만 아니라 열처리 중에는 일차입자 성장과 응집을 억제하여 열처리 온도와 시간에 따라 일차입자의 크기 뿐만 아니라 응집체으 크기도 제어할 수 있었다. 열처리 중 희석제는 고온에서 전구체 표면에서 치밀화 되어 일차입자 성장과 응집체 형성의 확산장벽으로 작용하는 것으로 판단되며 열처리 후 증류수에 쉽게 용해되어 $CeO_2$ 나노 입자 제조에 효과적이었다. 일차입자와 응집체의 크기 및 결정성은 희석제의 농도, 열처리 온도와 시간에 의존하는 것으로 확인 되었다.

시각적 되먹임 훈련이 만성 뇌졸중 환자의 기립 균형과 운동 기능에 미치는 영향

이동엽 ( Dong Yeop Lee ),이해현 ( Hae Hyun Lee ),송창호 ( Chang Ho Song ) 한국스포츠리서치 2007 한국 스포츠 리서치 Vol.18 No.4

The purpose of this study were to compared with visual feedback training with standing balance between the conventional standing balance training, the brain stroke patients over 6 months by assessed on the Sway Index in eyes open for stability(Sway EO), Weight Distribution Index(WDI), Time Up and Go Test(TUG), 10meter Walking Time(10m WT), Bohannon standing balance, MMAS for Motor function recovery. The following results were obtained. The Sway EO, WDI, 10m WT, standing balance is before to after training in the experimental group and control a group, it was a significance differences(p<.01)(p<.001). The TUG demonstrated significance differences before to after training in the experimental group and control group(p<.001) but no significant before to after training in the control group(p<.05). The motor function recovery were no significance differences between the before to after training in the experimental group and control group(p<.05). From what has been discussed above, we confirmed to improvement standing balance and gait capacity in chronic Stroke patients the visual feedback training with standing balance position and conventional standing balance training. Future study Considered need to be more the study relative to effect on postural balance control using visual feedback balance training.