양계산물 소비촉진협의회 만들어야

이해범 대한양계협회 1982 월간 양계 Vol.14 No.10

질산납이 한 국산 무미양서류의 배아발달에 미치는 영향

이해범,고선근 한국환경생물학회 2017 환경생물 : 환경생물학회지 Vol.35 No.4

An investigation of the effects of Pb for domestic anuran embryos were evaluated with the Frog Embryo Teratogenesis Assay; Xenopus (FETAX). Depending on the species, the difference between the embryo size and the embryonic development time was determined. As a result, mortality and malformation rates were increased, malformation patterns were changed and larval body length were decreased in a dose dependent manner of the Pb. The half maximal lethal concentration (LC50) of the Bufo gargarizans, Hyla japonica, Rana nigromaculata and Bombina orientalis were 0.58, 0.49, 0.52, 0.54 mg L-1, respectively. The half maximal effective concentration (EC50) of the Bufo gargarizans, Hyla japonica, Rana nigromaculata and Bombina orientalis were 0.35, 0.74, 0.30, 0.29 mg L-1, respectively. The teratogenic index (TI) were 1.66 in the Bufo gargarizans, 1.81 in the Hyla japonica, 1.73 in the Rana nigromaculata and 1.86 in the Bombina orientalis, respectively. Therefore, the Pb seems likely to have a teratogenic effect in all four species’ embryonic development. The Bombina orientalis was the most sensitive to the Pb. This means that the difference between the different species, even if they have all been exposed to the same concentration of pollutants depending on the species. The result above show that the Pb acts as a teratogenic agent in the development of the four domestic frog species. 국내에 서식하는 대표적인 무미양서류 중 두꺼비 (Bufo gargarizans), 청개구리 (Hyla japonica), 참개구리 (Rana nigromaculata) 및 무당개구리 (Bombina orientalis)를 대상 으로 각 종의 배아 발달에 미치는 Pb의 효과를 FETAX를 활 용하여 분석한 결과 4종의 배아 모두 Pb의 농도가 증가함에 따라 사망률, 기형율이 증가하고 다양한 기형 양상이 나타났 으며 성장률은 감소하였다. 두꺼비, 청개구리, 참개구리, 무 당개구리의 반수치사농도 (half maximal lethal concentration; LC50)는 각각 0.58, 0.49, 0.52, 0.54 mg L-1를 나타내었고 반 수영향농도 (half maximal effective concentration; EC50)은 각각 0.35, 0.27, 0.30, 0.29 mg L-1을 나타냈다. 기형성지수 (teratogenic index; TI=LC50/EC50)는 두꺼비, 청개구리, 참 개구리 및 무당개구리에서 각각 1.66, 1.81, 1.73, 1.86로 나 타나 무당개구리가 가장 민감한 영향을 나타냈으며 다음으 로 청개구리, 참개구리, 두꺼비 순으로 나타났다. 이는 서로 다른 종이 같은 농도의 오염물질에 노출되었다 하더라도 종 에 따라 차이가 나타날 수 있음을 의미한다. 이상의 결과를 통해 Pb가 무미양서류 4종의 배아 발달에 기형성 물질로 작 용함을 알 수 있었다.

실험적 담도폐색에 의한 혈액화학치의 변화

이해범,이병곤,전승기,김남수 한국임상수의학회 2008 한국임상수의학회지 Vol.25 No.5

The aim of this study was to establish an experiment animal model for easy diagnosis and monitoring effect of treatment of cholelithiasis by cholesterol and mixed gall stone. In 12 adult beagle dogs, five dogs there were in the control group and seven dogs in the experiment group with ligated cystic duct. General clinical signs were recorded, CBC and blood chemistry were examined. Control group and experiment group were evaluated every 2 weeks for a period of 20 weeks. The results were (1) Total blirubin, cholesterol, alanine aminotransferase (ALT), aspartate aminotransferase (AST) activity were significantly higher in the experiment group with ligated cystic duct than control group during 2-8 weeks. (2) Total alkaline phosphatase (TALP) activity was significantly higher when compare with control group during 2-8 weeks. Bone alkaline phosphatase(BALP) activity was increased slightly between 2 and 8 weeks but was decreased between 10 and 20 weeks. (3) Blood urea nitrogen (BUN) was decreased between 2 and 8 weeks. After 10 weeks, this was in normal range. (4) On complate blood count (CBC), Ca2+ and P were not changed until 20 weeks. (5) On clinical signs in experimental group, they observed a depression, yellow color of mucus membrane and conjunctiva, xanthinuria and xanthochromic. The present study showed that the experimental animal model with ligated cystic duct was a useful model to study biliary tract disease.

Radiographic Features of Traumatically Caused Retroflexion of Urinary Bladder in Two Dogs

이해범,강혜원,김민수,김남수,이기창 한국임상수의학회 2008 한국임상수의학회지 Vol.25 No.6

The urinary bladder retroflexion, flipped over backwards into the pelvic canal, by trauma has been rarely reported in dogs. This paper describes clinical and radiological features of urinary bladder retroflexion in two dogs with a history of traffic accident. The main clinical signs were hind limb lameness, severe pain and dysuria. Radiography and ultrasonography were performed to evaluate the patient’s damages. No remarkable findings were observed except pelvic fracture on survey radiograph. On cystourethrography, caudal displacement and retroflexion of the bladders were identified. Cystopexy was performed and the bladders were repositioned. After surgery one dog with severe azotemia and bilateral hydroureteronephrosis was deteriorated noticeably and euthanized by request of the owner, while the other dog was recovered no recurrence of the problem by 12 months post-surgery. It should be considered that contrast study and serial assessment in urinary tract be important to demonstrate the evidence of bladder retroflexion and evaluate the prognosis in dogs with severe trauma.

정상 개의 관절액에서 TRAP(Tartrate resistant acid phosphatase)농도 측정

이해범,알람,최성진,박상렬,이영훈,전승기,최인혁,김남수 한국임상수의학회 2005 한국임상수의학회지 Vol.22 No.3

The concentration of tartrate resistant acid phosphatase (TRAP) in synovial fluid of normal stifle joint was investigated in order to establish a baseline data to distinguish between healthy joint and joint with injury of cranial cruciate ligament (CCL). Twenty three mixed-breed healthy dogs free from joint diseases (fourteen adult and nine young) were used in this study. The dogs were sedated and synovial fluid was collected from the femoropatellar compartment of stifle joints by direct arthrocentesis. The concentration of TRAP in synovial fluid was determined using the method of Lang. The concentration of TRAP were 0.083±0.039IU/ml in adult dogs, 0.064±0.023IU/ml in young dogs, 0.075 ±0.028IU/ml in large dogs (>22kg), 0.076±0.046IU/ml in small dogs (<22kg), 0.085±0.036IU/ml in neutered dogs and 0.056±0.022IU/ml in intact dogs. The concentration of TRAP in the neutered dogs was significantly (p<0.05) higher than the intact dogs. This data can be used baseline data for a comparison with joint with injury of cranial cruciate ligament.

Evaluation of the Canine Stifle Joint after Transection of the Cranial Cruciate Ligament and Medial Collateral Ligament, and Medial Meniscectomy without Postoperative Exercise

이해범,정창우,김남수 한국임상수의학회 2007 한국임상수의학회지 Vol.24 No.3

period without postoperative exercise have a degenerative changes and also evaluated its suitability as an appropriateanimal OA models. Ten skeletally mature beagle dogs underwent a unilateral surgical transection of the cranial cruciateligament and, the medial collateral ligament as well as a medial meniscectomy. The contra-lateral joint was used ascontrol. After 12 weeks, After 12 weeks, the amount of joint damage, inflammation and biochemical change of synovialfluid was evaluated. Histological analysis showed chondrocyte clone formation, hypertrophy of the cartilage andmoderate los of proteoglycans in the experimental joints compared to control joints. In addition, the synovialinflammation in the experimental joints was observed. Bioc(matrix metalloproteinase) -2 and -9 in experimental joints compared to control joints. This canine OA model showsthe characteristics of degenerative joint disease, and may have a advantages of reducing the time and cost becausepostoperative exercise is not needed in this OA model.

키틴 유도체와 하이드록시아페타이트 복합체가 개의 뼈에 미치는 영향

이해범,신승호,김민수,이기창,정용식,김남수 한국임상수의학회 2008 한국임상수의학회지 Vol.25 No.5

The aim of this study was to investigate bioactivities of 50% Chitin - hydroxyapatite (Chitin-H) compound and 50% Chitosan - hydroxyapatite (Chitosan-H) compound in canine bone. Ten healthy mongrel dogs (1-5 years old, 1.7 - 6.9 kg) were used in this study. These compounds had been transplanted into bilateral femur separately, and then the changes of femur were observed through the examinations of hemato-biochemical profiles, radiology, and histological profiles for 42 days. After 3 weeks, expanded radiolucent changes were observed in both areas transplanted the compounds. After 6 weeks, the area transplanted the Chitin-H compound did not observe any changes of bony tissue, while the area inserted the Chitosan-H compound was observed changes of increasing bone formation. In histological examination, infiltrations of inflammatory cells and bone absorptions were observed at both transplanted sites. However an increasing of active osteogenesis was observed at the transplanted site with Chitosan-H compound. In conclusion, Chitosan-H compound had an function of active osteogenesis as compared with Chitin-H compound. From this study, it is indicated that Chitosan-H compound would be used in dogs with severe bone defect.

보건용 마스크 초미세먼지 제거 성능 평가 및 재사용 연구

이해범,김서정,주흥수,조희주,박기홍,Lee, Haebum,Kim, Seojeong,Joo, HungSoo,Cho, Hee-joo,Park, Kihong 한국입자에어로졸학회 2019 Particle and Aerosol Research Vol.15 No.4

In this study, performance (particle removal efficiency and breathing resistance) of several commercially available face masks (electrostatic filter masks (KF80 certified), a nanofiber filter mask (KF80 certified), and an uncertified mask) with their filter structure and composition were evaluated. Also, effects of relative humidity (RH) of incoming air, water and alcohol exposure, and reusability on performance of face masks were examined. Monodisperse and polydisperse sodium chloride particles were used as test aerosols. Except the uncertified mask filter, PM_2.5 removal efficiency was found to be higher than 90%, and the nanofiber filter mask had the highest quality factor due to the low pressure drop and high removal efficiency (nanofibers were arranged in a densely packed pore structure and contained a significant amount of fluorine in addition to carbon and oxygen). In the case of the KF80 certified mask, the removal efficiency was little affected when the RH of incoming air increased. When the mask filters were soaked in water, the removal efficiency of mask filters was degraded. In particular, the uncertified mask filter showed the highest removal efficiency degradation (26%). When the mask was soaked in alcohol, the removal efficiency also decreased with the greater degree than the water soaking case. The nanofiber mask filter showed the strongest resistance to alcohol exposure among tested mask filters. During evaluation of reusability of masks in real life, the removal efficiency of certified mask filter was less than 4% for 5 consecutive days (2 hours per day), while the removal efficiency of uncertified mask filter significantly decreased by 30% after 5 days.

살균제 Tebuconazole이 한국산 개구리류(두꺼비, 청개구리, 참개구리) 배아 발달에 미치는 영향

이해범,고선근,Lee, Hae-Bum,Ko, Sun-Kun 한국환경생물학회 2021 환경생물 : 환경생물학회지 Vol.39 No.3

국내에 서식하는 개구리들의 배아를 이용하여 살균제인 tebucoanzole의 독성을 파악하기 위해 FETAX (Frog Embryo Teratogenesis Assay-Xenopus) 기법에 따라 두꺼비(Bufo gargarizans), 청개구리(Hyla japonica), 참개구리(Pelophylax nigromaculatus)의 배아를 배양하면서 tebuconazole의 효과를 probit 분석법으로 조사하였다. 그 결과, tebuconazole의 농도에 의존하여 유생의 체장 길이는 감소하고 치사율과 기형률은 증가하였으며 tebuconazole의 teratogenic concentration (EC₅₀)은 각각 34.4, 10.6, 14.9 mg kg^-1을 나타내었고 embryo lethal concentrations (LC₅₀)은 75.4, 38.2, 39.6 mg kg^-1 을 나타내었다. Teratogenic index (TI=LC₅₀/EC₅₀)는 각각 2.19, 3.58, 2.65을 나타내어 두꺼비, 청개구리, 참개구리 배아 발달에 최기형성 물질로 작용함을 알 수 있었다. 이상의 결과들로 보아 tebuconazole은 낮은 농도에서 개구리 배아의 발달에 민감하게 반응하였으며 치사율, 기형률, 성장률, 기형양상 등을 기존의 연구들과 비교하였을 때 유사한 결과를 나타내어 국내 서식하는 개구리류 배아발달에 영향을 미칠 수 있는 것으로 여겨지며, 종에 따라 치사율 및 기형률, 기형양상 등의 차이를 나타내는 원인 등을 명확히 파악하기 위해서 종 특이적 특성 등을 규명하는 연구가 더 필요할 것으로 여겨진다. In this experiment, we investigated the toxicity of tebuconazole (fungicide) using domestic frog embryos, along the FETAX (Frog Embryo Teratogenesis Assay-Xenopus) protocol. Bufo gargarizans, Hyla japonica, and Pelophylax nigromaculatus embryos were incubated, and investigation of the tebuconazole effect was performed by the probit analysis. As a result, depending on the concentrations of tebuconazole, the mortality and malformation rates were increased and larval body length was decreased. The teratogenic concentrations (EC₅₀) of tebuconazole were 34.4mg L^-1, 10.6mg L^-1, and 14.9mg L^-1, respectively, and the embryo lethal concentrations(LC₅₀) of tebuconazole were 74.7 mg L^-1, 38.5 mg L^-1, and 39.1 mg L^-1, respectively. The teratogenic index (TI) valuesof tebuconazole were 2.19, 3.58, and 2.65; thus, it showed teratogenicity in embryonic development of these three frogs. These results revealed that in this experiment, tebuconazole suppressed the development of embryos at a relatively low concentration. In addition, mortality, malformation ratios, malformation patterns, and growth rates were similar to the results from the other assay systems. Therefore, tebuconazole was thought to have an effect on the embryo development of domestic frogs. In future, it will be necessary to identify species specificity in order to the clarify the causes of differences in mortality, malformation rate, and malformation patterns depending on the species.

전염소처리와 한외여과를 이용한 상수처리에서 철과 망간의 제거

이해범,한정욱,추광호,최상준 한국공업화학회 2002 응용화학 Vol.6 No.2

This study mainly focused on the removal of iron and manganese from water sources by a crossflow ultrafiltraion (UF) process in combination with a prechlorination step using different chlorine dosage, along with evaluation of removal efficiencies for natural organic matter (NOM) and turbidity. For feed water containing 1.0 mg/L as Fe and 0.5 mg/L as Mn, substantial removal of Fe was achieved without chlorination, possibly because of the oxidation of ferrous iron to ferric iron by dissolved oxygen (DO) and consequent precipitation. However, there was no Mn removal in the absence of chlorine but with addition of chlorine, Mn removal increased dramatically and reached more than 80% at a chlorine dose of 3 mg/L. In contrast, with a higher chlorine dose (e.g., 5 mg/L as Cl_2), further Fe and Mn removal was not significant, but serious membrane fouling was occurring. However, it was demonstrated that NOM removal substantially increased in the presence of Fe and Mn by chlorination. Presumably, it was believed that Fe or Mn oxides formed by chlorination played a role in NOM removal by adsorption.