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Antiplatelet, anticoagulant, and profibrinolytic activities of baicalin
이원화,구새광,배종섭 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.5
Baicalin is a flavonoid compound purified fromthe medicinal plant Scutellaria baicalensis Georgi and hasbeen reported to possess anti-inflammatory and anti-viralactivities. However, antiplatelet, anticoagulant, and profibrinolyticproperties of baicalin have not been studied. Inthis study, the anticoagulant and antiplatelet activities ofbaicalin were measured by monitoring activated partialthromboplastin-time (aPTT), prothrombin time (PT), theactivities of cell-based thrombin and activated factor X(FXa), platelet aggregation and thrombus formation. Theeffects of baicalin on the expressions of plasminogenactivator inhibitor type 1 (PAI-1) and tissue-type plasminogenactivator (t-PA) were also tested in tumor necrosisfactor-a (TNF-a) activated human umbilical vein endothelialcells (HUVECs). Our data showed that baicalininhibited thrombin-catalyzed fibrin polymerization andplatelet functions, prolonged aPTT and PT significantlyand inhibited the activities and production of thrombin andFXa. Baicalin also prolonged in vivo bleeding time andinhibited TNF-a induced PAI-1 production. Furthermore,PAI-1/t-PA ratio was significantly decreased by baicalin. Collectively, these results indicate that baicalin possessesantithrombotic activities and suggest that the current studycould provide bases for the development of new anticoagulantagents.
The Decursin Analog, CYJ-27, Suppresses Inflammation Via the Downregulation of NF-κB and STAT-1
이원화,심현채,최윤정,서주영,윤미영,송규용,배종섭 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.8
CYJ-27, a synthetic analog of decursin, prevents the generation of proinflammatory cytokines and oxidative stress. In this study, the effects of CYJ-27 on the regulation of inducible nitric oxide synthase (iNOS), heme oxygenase (HO)-1, and cyclooxygenase (COX-)2 were characterized in lipopolysaccharide (LPS)-treated human umbilical vein endothelial cells (HUVECs). In addition, the effects of CYJ-27 on the production of iNOS and representative proinflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-1β, were tested in the lung tissues of LPS-treated mice. CYJ-27 promoted the expression of HO-1, suppressed NF-κB-luciferase activity, and reduced COX-2/PGE2 and iNOS/NO, resulting in a diminution in phosphorylated-STAT-1. Furthermore, CYJ-27 promoted the nuclear translocation of Nrf2, enhanced the combination of Nrf2 to antioxidant response elements, and diminished IL-1β production in LPS-activated HUVECs. CYJ-27-downregulated iNOS/NO expression was rescued after the RNAi suppression of HO-1. In LPS-treated mice, CYJ-27 significantly diminished iNOS production in the lung tissues and TNF-α expression in the bronchoalveolar lavage fluid. These findings indicate that CYJ-27 exerts anti-inflammatory activities by regulating iNOS through downregulation of both NF-κB activation and phosphorylated-STAT-1. Hence, it can act as a template for the development of novel substances to treat inflammatory diseases.
RESEARCH ARTICLE Emodin-6-O-b-D-glucoside down-regulates endothelial protein C receptor shedding
이원화,구세광,배종섭 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.9
Endothelial protein C receptor (EPCR) plays animportant role in the protein C anticoagulation pathwayand in the cytoprotective pathway. Previously, EPCR canbe shed from the cell surface, which is mediated by tumornecrosis factor-a converting enzyme (TACE). SolubleEPCR levels are increased in patients with systemicinflammatory diseases. Recently, we reported that a newactive compound, emodin-6-O-b-D-glucoside (EG) fromReynoutria japonica, has anti-inflammatory activities. However, little is known of the effects of EG on EPCRshedding. Here, we investigated this issue by monitoringthe effects of EG on the phorbol-12-myristate 13-acetate(PMA) or the cecal ligation and puncture (CLP)-mediatedEPCR shedding and its underlying mechanisms. Datashowed that EG potently inhibited the PMA and CLPinducedEPCR shedding by suppressing TACE expression. Given these results, EG could be used as a candidatetherapeutic for the treatment of vascular inflammatory diseases.
이원화 대한영상의학회 1983 대한영상의학회지 Vol.19 No.4
The injuries to the anlke joint complex can cause not only bony fractures but also associated ligament rupture. Understanding of the mechanism of those injuries is important for the radiologist to give more information ot the orthopedist to identify ligament injuries and then to fix the fractures. In 1948, Lauge-Hansen devised a classification of ankle fractures based on the position of the foot and direction of injuring force at the time of injury. On the basis of location and appearance of the fibular fracture, 4 types of injuries are identified ; pronation-abduction (PA), pronation-external rotation (PER), supination-adduction (SA), and supination-external rotation (SER). 60 cases of ankle fractures treated at Kyung Pook National University Hospital from Jan. 1973 to Dec. 1982 were analized an dclassified. The results are as follows : 1. Male was more frequently affected than female and the sex ratio was 4 : 1. 2. The most commonly affected age group was 20's. 3. Right side was more frequently affected than left. 4. A mong 4 types, the most common was SER type (33.3%) and next were PER (30.3%), SA (16.7%), and PA (13.3%). types.