들깨 종간 교잡(Perilla citriodora × P. hirtella) 집단의 양적 형질 유전자좌 분석

이명희(Myoung Hee Lee),오기원(Ki Won Oh),김명식(Myung Sik Kim),김성업(Sung Up Kim),김정인(Jung In Kim),오은영(Eun Young Oh),배석복(Suk Bok Pae),여운상(Un Sang Yeo),김태호(Tae Ho Kim),이정희(Jeong Hee Lee),정찬식(Chan Sik Jung),곽도연(Do 한국육종학회 2018 한국육종학회지 Vol.50 No.1

레몬들깨(P. citriodora)와 범꼬리소엽(P. hirtella) 등 2종의 2배체 야생 들깨 종간 교잡을 통해 들깨 유전체 사업의 연관 유전지도 작성에 필요한 집단을 육성하였다. 두 종의 염색체 수는 n=10으로 같고 종간 교잡 후대는 세대를 거치는 동안 정상적인 생육과 결실을 보였다. F2 집단의 표현형은 정규분포를 보여 종간 교잡 식물체의 감수분열 과정에서 두 종의 염색체가 친화성이 높은 것으로 보였다. F2 집단으로부터 수확한 종자에서 Rosmarinic acid, Luteolin, Apigenin등 3종의 성분을 분석한 결과 4배체 재배종 들깨의 함량과 유사한 범위에 분포하였고 Apigenin과 Luteolin 간에 0.762의 높은 정의 상관이 있었다. 주요 농업형질 및 기능성분 분석 결과 21개의 QTL을 찾았다. Apigenin과 Luteolin 간에 높은 정상관이 있었으나 QTL 영역은 다르게 조사되었다. 엽색(LC)의 QTL은 3번 염색체에 하나만 존재하였는데 LOD가 14.3으으로 매우 높고 표현형 변이에 대한 QTL의 설명 비율이 50.4%로 높은 부분 우성 형질이었다. 또한 이 QTL 주변 영역에는 안토시아닌 생합성 조절인자로 알려진 basic helix-loop-helix(bHLH) DNA-binding superfamily protein, myb domain protein 43, Transducin/WD40 repeat-like superfamily protein이 확인되었다. An interspecific cross between P. citriodora and P. hirtella constitutes ideal material for a linkage map construction in genome project of Perilla. The chromosomes of the species are same with n=10 and progenies of the cross are normal in growth and seed set. The phenotype of F2 population of the cross are normally distributed and this is a proof of high affinity of the chromosomes during their sexual reproduction. Rosmarinic acid, Luteolin and Apigenin contents of F2 plants were distributed in similar range with tetraploid perilla cultivars. Luteolin and Apigenin are positively correlated with correlation coefficient of 0.762. 21 QTLs were detected in agronomic traits and Rosmarinic acid, Luteolin and Apigenin contents. Even though Luteolin and Apigenin are positively correlated, QTLs were located in different position. Purple leaf color related QTL was mapped in Chromosome 3 with LOD of 14.3, PVE of 50.4%. Three anthocyanin biosynthesis transcription factor like sequences, WD40 repeat-like superfamily protein, myb domain protein 43 and basic helix-loop-helix (bHLH) DNA-binding superfamily protein, were detected near from the QTL.

중형 고추 수확기 개발을 위한 기초연구 - 드럼형 선별부 요인 시험 -

이명희 ( Myung Hee Lee ),강영선 ( Young Sun Kang ),신서용 ( Seo Yong Shin ),양은영 ( Eun Young Yang ),김대철 ( Dae Cheol Kim ) 한국농업기계학회 2019 한국농업기계학회 학술발표논문집 Vol.24 No.1

고추는 매운맛과 향을 가지고 있어 여러 음식의 주 양념으로 사용되며, 국내 양념채소 점유율의 22%를 차지하는 중요한 작물이다. 그러나 우리나라의 고추 재배면적은 2013년 45,360ha에서 지속적인 감소 추세를 보이며 2018년 28,824ha까지 감소하였다. 더불어 지구온난화로 평균 기온이 증가해 초기 생육이 부진하고 잦은 비로 인한 병충해 피해 등으로 연 평균 고추 가격은 상승하고 있다. 또한 농촌 농가의 인구감소와 고령화로 인해 노동력이 부족한 상황이다. 이러한 추세가 지속된다면 국내 고추 산업은 붕괴 될 우려가 있어, 고추 산업의 생력화를 위해 고추수확작업의 기계화가 절실히 필요하다. 이에 T사는 소규모의 농가가 대부분을 차지하고 있는 국내 실정에 맞춘 중형의 고추수확기를 개발 중에 있다. 현재 개발 중인 수확기계로 수확 시 다량의 줄기부착과가 유입되어 수확 후 추가 노동력이 발생되는 상황이다. 따라서 본 연구는 줄기 분리 장치인 드럼형 선별부에 있어서 3개 형태의 선별부와 고추를 이송하는 컨베이어 벨트의 회전 속도에 따른 선별율을 분석하고자 요인 실험을 실시하였다. 시험 시료는 국립원예특작과학원 온실에서 재배된 AR레전드 품종으로 실험 당 3반복으로 각 5주를 사용하였고, 각 주를 제일 아래 분지에서 4개로 나누어 실험하였다. 시험 조건은 총 4안으로 1안은 최초 설계 속도인 1번 드럼 78rpm, 2번 드럼 148 rpm, 3번 드럼 222rpm, 컨베이어 266 rpm으로 실험하였고, 2안은 1번 드럼 89 rpm, 2번 드럼 254 rpm, 3번 드럼 178rpm, 컨베이어 228 rpm으로 2번 드럼의 회전 속도를 높였다. 3안은 1번 드럼 111rpm, 2번 드럼 211 rpm, 3번 드럼 317rpm, 컨베이어 202 rpm로 3개의 드럼 속도를 높이고 컨베이어 속도를 낮추어 실시하였고, 4안은 1번 드럼 278rpm, 2번 드럼 793rpm, 3번 드럼 370rpm, 컨베이어 173 rpm으로 3개의 드럼 속도를 전체적으로 높인 후 2번 드럼의 회전 속도를 추가적으로 높여 실험하였다. 회전속도에 따른 줄기 분리 장치 요인시험 결과, 1안이 정상과율 51.6%, 줄기부착과율 45.1%, 손상과율 3.3%로 성능이 가장 낮았으며, 4안이 정상과율 70.0%, 줄기부착과율 27.7%, 손상과율 3.0%로 성능이 가장 높았다. 요인 실험 결과를 종합적으로 보았을 때 회전 속도가 높아질수록 성능이 높아지는 것을 볼 수 있으며, 현 실험 조건보다 더 높은 속도에서 추가 실험을 해볼 필요성이 있다고 판단된다.

녹용의 약효 성분에 관한 연구 ( 3 ) 녹용 및 Pantocrin 의 지방산 조성에 관하여

김영은,이승기,이명희,신승언 ( Young Eun Kim,Seung Ki Lee,Myoung Hee Lee,Seung Uon Shin ) 생화학분자생물학회 1976 BMB Reports Vol.9 No.4

The lipid soluble fraction of antler velvet layer (Cervus nippon taiouanus) was extracted and compared to that of pantocrin (ethanol preparation of antler, commericially available). The lipid soluble components(801.5㎎ from antler velvet layer and 979.1㎎/150㎖ from pantocrin) were fractionated into neutral lipids, glycolipids, phosphclipids, gangliosides, and nonlipid components (lipoprotein and peptide) on Sephadex G-25 column followed by silicic acid column chromatography. The percentage composition of the lipid soluble fraction was neutral lipid 65.3%, glycolipid 5.2%, phospholipid 12.9%, ganglioside 6.1 %, nolipid 2.9 %. The neutral lipids were fractionated into squalene, sterolester, triglyceride, free fatty acid, sterol, diglyceride, monoglyceride on boric acid impregnated silicic acid column. The fractions collected were determined by thin layer chromatography. The compositions of the free fatty acid fractions were determined by gas liquid chromatography. The sterolester, triglyceride fractions were hydrolyzed to give free fatty acids with 10% KOH solution, refluxed at 80-85℃ for four hours, and carried out gas liquid chromatography. The free fatty acids in antler velvet layer were found to be nine kinds of saturated fatty acids, nine kinds of unsaturated fatty acids, two branched fatty acids and six unknown components. The main components of antler free fatty sacid were oleic (21.7%), linolenic(31.2%), palmitic(19.6%), stearic(12.1%), palmitoleic(10.2%), and those make 94.4% of antler free fatty acids. In pantocrin free fatty acid fraction, seven kinds of saturated fatty acids, four kinds of unsaturated fatty acids, one branched fatty acid and three unknown compopents were detected. The main components of pantocrin free fatty, acid fraction wereoleic(45%), palmitic(19.5%), lauric(10.9%), stearic(6.6%), palmitoleic(5.6%), myristic(3.4%), capric(3.1%), and those make 94.1% of pantocrin free tfatty acids. The ester fatty acids of triglyceride fraction were found as follows: In antler triglyceride faction, nine kinds of saturated fatty acids, three kinds of unsaturated fatty acids, three branched fatty acids and ten unknown components were detected. In pantocrin triglyceride fraction, it was found to contain five kinds of saturated fatty acids, two kinds of unsaturated fatty acids, one branched fatty acid and ten unknown components. after saponification of triglyceride fraction, two new fatty acids (pentadecylic, oleic) in antler neutral lipid and two new fatty acids (lauric, dodecenoic) in pantocrin neutral lipid were detected. In sterol ester fraction, there were six saturated fatty acids, three unsaturated fatty acids, one branched fatty acid and eight unknown components in antler neutral lipid, and five saturated fatty acid, three unsaturated fatty acids and five unknown components in pantocrin neutral lipid. After saponification of sterol ester fractions, new fatty acids were determined as dodecenoic, lauric, anteiso-pentadecylic, isopalmitic, heptadecanoic in antler sterol ester fraction and dodecenoic, palmitic, heptadecanoic, heptadecenoic in pantccrin sterol ester fraction.

녹용의 약효 성분에 관한 연구(IV) 녹용 벨?殼?의 프로스타그란딘의 검출

김영은,이승기,이명희,Kim, Young-Eun,Lee, Seung-Ki,Lee, Myoung-Hee 생화학분자생물학회 1977 한국생화학회지 Vol.10 No.1

녹용의 지용성 성분에 관한 생화학적 연구의 일환으로 대만산 꽃사슴(cervus nippou taiouanus)의 녹용을 절단한 후 동결하여 벨?殼?과 해면상 골조직으로 분리하였다. 벨?殼?을 절편으로 만든 후 0.9% saline 용액중에서 Virtis homogenizer로 파쇄하여 Jouvenaz 의 방법에 따라 prostaglandin을 추출하였다. Anderson, Samuelsson 등의 방법에 준하여 thin layer chromatography를 행하여 $PGE_2$, 15-epi-$PGE_1$, $PGE_{1{\alpha}}$ 및 $PGE_{1{\beta}}$로 추정되는 물질을 검출하였으며 preparative T.L.C. 를 행하여 분리한 후 Samuelsson의 방법에 따라 U.V. spectroscopy를 행하여 $PGE_2$, 15-epi-$PGE_1$으로 추정되는 분획에서는 알카리 처리 후에 278nm에서 U.V. 흡수를 나타냈으며 한편 $PGE_{1{\alpha}}$, $ PGE_{1{\beta}}$로 추정되는 분획에서는 알칼리 처리 전후에 U.V. 흡수가 없었으므로 T.L.C의 결과와 U.V. spectroscopy의 결과는 상호간에 일치됨을 알 수 있었다. 또한 Fishbein 등의 방법에 의한 gas-liquid chromatography를 행하여 $PGE_2$ standard peak와 일치하는 peak를 얻어 $PGE_2$의 존재를 확인하였으며 retention time이 $PGE_{1{\alpha}}$ 및 $PGE_{1{\beta}}$와 일치되는 peak를 얻어 세방법에서 공히 $PGE_2$, 15-epi-$PGE_1$, $PGE_{1{\alpha}}$, $PGE_{1{\beta}}$의 존재를 검출하였다. The fresh antler was cut, frozen and mechanically separated into spongy bone layer and velvet layer. The prostaglandin-like components were extracted from antler velvet layer and qualitatively analyzed by three different methods: thin layer chromatography, ultra-violet spectroscopy after conversion to PGB series by alkaline treatment, and gas liquid chromatography. The prostaglandins detected were $PGE_2$, 15-epi-$PGE_1$, $PGE_{1{\alpha}}$, $PGE_{1{\beta}}$. The experiments indicated that the petroleum ether fraction contained mostly PGE series and that the ethylether fraction contained PGF series. One major prostaglandin-like components of antler velvet layer was found to be the main compound in the petroleum ether extract. The structure of this compound is still unknown but the gas liquid chromatography data suggest that it is a hydroxy fatty acid.

설포라제 캡슐(아세브로필린 100㎎)에 대한 부로필 캡슐의 생물학적 동등성

조혜영 ( Hea Young Cho ),박은자 ( Eun Ja Park ),강현아 ( Hyun Ah Kang ),김세미 ( Se Mi Kim ),박찬호 ( Chan Ho Park ),오인준 ( In Joon Oh ),임동구 ( Dong Koo Lim ),이명희 ( Myung Hee Lee ),이용복 ( Yong Bok Lee ) 전남대학교 약품개발연구소 2005 약품개발연구지 Vol.14 No.-

녹용의 약효 성분에 관한 연구 ( Ⅳ ) 녹용 벨벳층의 프로스타그란딘의 검출

김영은,이승기,이명희 ( Young Eun Kim,Seung Ki Lee,Myoung Hee Lee ) 생화학분자생물학회 1977 BMB Reports Vol.10 No.1

The fresh antler was cut, frozen and mechanically separated into spongy bone layer and velvet layer. The prostaglandin-like components were extracted from antler velvet layer and qualitatively analyzed by three different methods: thin layer chromatography, ultra-violet spectroscopy after conversion to PGB series by alkaline treatment, and gas liquid chromatography. The prostaglandins detected were PGE₂, 15-epi-PGE₁, PGF_(1α), PGF_(1β). The experiments indicated that the petroleum ether fraction contained mostly PGE series and that the ethylether fraction contained PGF series. One major prostaglandin-like components of antler velvet layer was found to be the main compound in the petroleum ether extract. The structure of this compound is still unknown but the gas liquid chromatography data suggest that it is a hydroxy fatty acid.

역병에 강하고 수량성이 높은 참깨 품종 “건백”

김성업(Sung Up Kim),이명희(Myoung Hee Lee),배석복(Sug Bok Pae),오은영(Eun Young Oh),김정인(Jung In Kim),하태정(Tae Jung Ha) 한국육종학회 2018 한국육종학회지 Vol.50 No.3

A sesame variety “Goenbaek” (Sesamum indicum L.) with phytophthora disease resistance and high yield was developed in 2013. It was obtained via a cross between “Sungbun (IT156459)” and “SIG950006-4-1-1” in 2001. “Goenbaek” has few branches and triple capsules per node. Notably, “Goenbaek” exhibited resistance to phytophthora blight disease and lodging in the field. Furthermore, the yield of “Goenbaek” was about 1.16 ton per hectare, which was 12% higher than that of “Yangbaek”. “Goenbaek” also showed a high oil content of 50.2%.

내병성 다수성 흰색 참깨 품종 ‘유미’

김성업(Sung-Up Kim),이명희(Myoung-Hee Lee),배석복(Sug-Bok Pae),오은영(Eun-Young Oh),김정인(Jung-In Kim),하태정(Tae-Jung Ha) 한국육종학회 2020 한국육종학회지 Vol.52 No.1

Sesame (Sesamum indicum L.) is a traditional oil seed crop grown in Korea. Sesame cultivation in Korea, however, is difficult and the yield is still very low. As such, a sesame variety, ‘Yoomi,’(Sesamum indicum L.) with disease resistance and high yield was developed in 2012. It was obtained by crossing ‘Yangbaek’ and SIG950480-6-3-1 in 2001. ‘Yoomi’ has few branches and a triple capsule per node. Notably ‘Yoomi’ exhibited a resistance to Phytophthora blight disease and a lodging resistance in the field. The yield of ‘Yoomi’ was about 1.06 ton per hectare, 8% higher than that of ‘Yangbaek’. ‘Yoomi’ crude fat content was 49.7%, and lignan content was 6.62 mg/g. This variety will contribute to increasing sesame production in Korea and will be utilized in sesame breeding programs.

난소암 조직에서 Mycoplasma DAN 존재의 의의 : PCR-ELISA 기법을 이용한 검출 Detection by PCR-ELISA Technique

이명희,남주현,김용만,김종혁,목정은,나준희,김영탁,엄재영 대한부인종양 콜포스코피학회 1998 Journal of Gynecologic Oncology Vol.9 No.1

Mycoplasmas, cell wall-less bacteria of class Mollicutes, are among the smallest self-replicating organisms known and reside ubiquitously at the cell membrane or internalized into the cell. They mimic viruses in many of their activities and further they may have oncogenic activity. The oncogenic potential of mycoplasmas was only recently realized when they were shown to cause chromosomal changes and in vitro cell transformations through gradual progressive chromosomal loss and translocations. The association between these organisms and human cancers has been evaluated and actually mycoplasmas were detected in 50% of gastric cancers. In gynecologic cancer, one study demonstrated a 59.3% prevalence rate of mycoplasmas in malignant ovarian tumors but the explanations for the association between the organisms and ovarian cancer might be somewhat confusing, at least in part, due to absence of normal control. The present objective was to determine the presence of mycoplasma DNA in ovarian cancer tissues and normal ovary in Korea. Fresh frozen tissue samples stored at -72 ℃were used for mycoplasma DNA assay. The study materials comprised twenty-nine human ovarian cancer tissues and ten normal ovarian tissues. After extraction of DNA, the combined PCR-ELISA(polymerase chain reaction and enzyme linked immunosorbent assay) procedure was performed with consensus primers targeting for 15 species of mycoplasmas and acholeplasmas together with negative and positive controls, which was known as very sensitive method. The results showed mycoplasma DNA were present in none of normal ovarian tissue and in 13.8%(4 of 29) of the ovarian cancer specimens, which is much lower than that of the previousstudy. Three positive cases showed very strong reactivities, but there was no significant correlation between presence of mycoplasma DNA and the clinicopathological characteristics of the patients. These results suggest that mycoplasma can not be the contributor in the mechanism of carcinogenesis in the most of ovarian cancers in Korea,ut the association between mycoplasma and ovarian cancer is worth to be investigated.

관절염 대상자의 건강상태 조사 : 일개 도ㆍ농 복합시를 중심으로

이은희,최선하,서길희,소애영,이경숙,최정숙,고경덕,김명천,박현자,백승리,유보비,윤현숙,이애란,이명희,이은숙,임영옥,정복녀,이영숙 대한류마티스 건강전문학회 2001 근관절건강학회지 Vol.8 No.2

The Purpose of this study was to investigate the health status and to examine the relationship between health related variables of the clients with arthritis. This descriptive-correlational study was measured physical health status(pain, fatigue, physical disability, joint flexibility) and psycho-social health status(social function disability, self-efficacy, depression) . The clients were consisted of 127 arthritis clients who were randomly selected from health center, 2 sub health center and 6 primary health care center in Wonju City. The data was collected from November 2000 to January 2001. The results of the study analyzed using a SPSS win were as follows : 1) The subjects' average age was 64.81 years old(female : 86.6%, male : 13.4%). 2) In physical health status, the average score of fatigue was 63 scores and pain was 69.77 Physical disability score was higher than total disability score. In psycho-social disability, housekeeping management disability score was higher than social function disability score. In psycho-social health status, the average score of self-efficacy was 70.86 and the subjects have higher level of depression, more than 20 scores. 3) On Pain sites, almost of subjects complained of a knee joint pain. The waist, wrist, and shoulder joint Pain were followed. The numbers of Pain site were 8.3 Per individuals 4) The flexibility of knee and shoulder joint were lower than another joints. 5) Correlation with fatigue, pain, physical disability, social disability, self-efficacy and depression was revealed highly significant. We suggest that health Promotion program considering our research results should be provided for arthritis clients in community level .