In vitro에서 핵산치환인자 BAP이 단백질-분자 샤페론 복합체 해리에 미치는 영향

이명주,김동은,이태호,정영기,김영희,정경태,Lee Myoung-Joo,Kim Dong-Eun,Lee Tae-Ho,Jeong Yong-Kee,Kim Young-Hee,Chung Kyung-Tae 한국생명과학회 2006 생명과학회지 Vol.16 No.3

소포체는 세포막의 합성뿐만 아니라 세포막에 존재하거나 세포외로 분비되어져야 할 단백질을 합성하는 세포내 소기관이다. 소포체에서 단백질이 합성되어질 경우 이황화결합이 형성되고 glycosylation 등의 수식이 일어나며, 이와 동시에 folding과 assembly과정을 거쳐 삼차원적 구조로 성숙이 되는데 이 과정은 folding enzyme과 molecular chaperone의 도움을 받아 이루어진다. 소포체 내에 존재하는 molecular chaperone 중 가장 잘 알려진 것으로 BiP이 있다. BiP의 기능은 N-terminus의 ATPase domain에 의해 조절되고 ATPase domain은 이것과 선택적으로 결합하는 조절인자에 의해 ATPase의 활성이 영향을 받는다. BiP의 핵산치환조절인자로서 발견된 BAP은 ATPase domain에 결합된 ADP를 ATP로 치환하는 것으로 기능이 알려져 있다. 이 BAP의 핵산치환기능이 BiP의 샤페론 작용에 어떤 영향을 미치는지를 in vitro에서 항체 heavy chain을 이용하여 알아보았다. BAP은 ATP보다 ADP가 결합되어 있는 BiP과 더 잘 결합을 하며, in vitro에서 BiP과 결합하고 있는 unfolded 단백질을 BAP은 BiP으로부터 해리하였다. 또한 소포체내에 존재하는 Hsp70 homologue chaperone인 BiP과 Grp170에 대한 BAP의 결합특이성을 anti-Grp170과 anti-BAP 항체로 co-immunoprecipitation을 하여 확인한 결과 BAP은 Grp170과 결합을 하지 않았다. 따라서 BAP은 ER내에 존재하는 동일한 family group에 속하는 Grp170과 BiP에 대하여 BiP에만 특이성을 갖는 것으로 나타났다. Molecular chaperones and folding enzymes in the endoplasmic reticulum (ER) associate with the newly synthesized proteins to prevent their aggregation and help them fold and assemble correctly. Chaperone function of BiP, which is a Hsp70 homologue in ER, is controlled by the N-terminal ATPase domain. The ATPase activity of the ATPase domain is affected by regulatory factors. BAP was identified as a nucleotide exchange factor of BiP (Grp78), which exchanges ADP with ATP in the ATPase domain of BiP This study presents whether BAP can influence folding of a protein, immunoglobulin heavy chain that is bound to BiP tightly. We first examined which nucleotide of ADP and ATP affects on BAP binding to BiP The data showed that endogenous BAP of HEK293 cells prefers ADP for binding to BiP in vitro, suggesting that BAP first releases ADP from the ATPase domain in order to exchange with ATP. Immunoglobulin heavy chain, an unfolded protein substrate, was released from BiP in the presence of BAP but not in the presence of ERdj3, which is another regulatory factor for BiP accelerating the rate of ATP hydrolysis of BiP The ADP-releasing function of BAP was, therefore, believed to be responsible for immunoglobulin heavy chain release from BiP. Grp170, another Hsp70 homologue in ER, did not co-precipited with BAP from $[^{35}S]$-metabolic labeled HEK293 lysate containing both overexpressed Grp170 and BAP. These data suggested that BAP has no specificity to Grp170 although the ATPase domains of Grp170 and BiP are homologous each other.

창원지역 치과위생사의 직무스트레스와 직무만족에 관한 연구

이명주,권선화,구효진,Lee, Myeong-Ju,Kwon, Sun-Hwa,Goo, Hyo-Jin 한국임상보건과학회 2013 한국임상보건과학회지 Vol.1 No.3

Purpose. This study was performed on survey related dental hygienist job stress and job satisfaction, to improve the work efficiency of the dental hygienist and for dental hygienist to eliminate the cause of stress for health promotion and for management to be able to help to finding ways to provide basic data. Method. This survey is based on 235 people who are dental hygienists working in Changwon city from November 1 to November 30 2012, by statistical analysis of collected data using SPSS Win Program 20.0 version t-test, univariate analysis of variance (one-way ANOVA), correlation analysis that performed to determine statistical significance with a significance level of 0.05 was considered. Results. For correlation of job satisfaction and job stress, the job demands, job insecurity, and workplace culture shows a negative correlation. For Correlation of the physical environment, insufficient job control, interpersonal conflict, and organizational system is, lack of reward shows a positive correlation. The less job stress due to Insufficient job control, interpersonal conflict, organizational system, is, the higher job satisfaction due to job demands and workplace culture is.(p <0.05), The higher job stress is, the higher the job satisfaction is(p <0.05). Conclusions. Job satisfaction and job stress may be relevant each other, there fore several measures should be sought to solve insufficient job control, interpersonal conflict, organizational system and the lack of reward, consequently to increase the job satisfaction of dental hygienists.

CRT용 GLASS 제조공정 이해

이명주,Lee, Myeong-Ju 한국정보디스플레이학회 2001 한국정보디스플레이학회지 Vol.2 No.5

제주 고산 지역의 에어러솔 수농도 변동과 기상요소와의 상관성 연구 : 2001-2003

이명주,오성남,김은연,장기호,Lee, Myoung-Joo,Oh, Sung-Nam,NhoKim, Eun-Yun,Chang, Ki-Ho 한국방재학회 2006 한국방재학회논문집 Vol.6 No.3

기후변화와 관련한 한반도 배경대기의 에어러솔에 대한 특성 연구는 관측된 입자 수농도와 대기 복사 관측 자료를 바탕으로 에어러솔의 물리적, 화학적 및 광학적 특성에 대하여 분석하였다. 에어러솔의 물리적 특성 연구는 2001년부터 2003년까지 제주도 고산관측소에 설치된 광학입자계수기(Optical Particle Counter, OPC)로 $0.3{\sim}25{\mu}m$직경의 에어러솔을 8개 구간으로 나누어 관측하여 분석하였다. 그 결과 황사의 발생률이 높은 봄철에 조대 입자($2.2{\mu}m$이상)의 수농도가 다른 계절보다 높게 나타났다. 기상요소 (상대습도, 풍속, 풍향, 시정 등)와 입자 수농도의 상관관계 분석에 의하면 $0.3{\sim}0.5{\mu}m$ 크기 구간에서는 상관성이 없는 것으로 나타났으며 $0.5{\sim}2.23{\mu}m$ 구간에서는 에어러솔 수농도와 상대습도가 서로 양의 상관관계를 가지는 것으로 나타났다. 풍속과의 상관성에서 작은 입자들은 풍속이 강해질수록 감소하는 패턴으로 분석되었으며 겨울철에는 조대입자 수농도가 높은 풍속에 증가하는 것으로 나타났다. 그리고 시정거리는 $0.5{\sim}1{\mu}m$ 크기 정도의 에어러솔 수 농도와 밀접한 관련이 있는 것으로 나타났으며 에어러솔 수 농도가 증가할수록 시정거리는 감소하는 경향을 보였다. With the aim to develop the monitoring technology on background atmosphere and climate change over Korean Peninsula, observations and studies on chemical, physical and optical properties of the atmospheric aerosols are made. Aerosol number concentration are measured with Optical Particle Counter from 2001 to 2003 at Gosan for 8 size intervals from 0.3 to $25{\mu}m$ diameter range. For the seasonal variation, the number concentration of coarse particles in spring at Gosan was higher than other seasons due to the influence of sand storm in spring. There is no significant correlations between fine particles ($0.3{\sim}0.5{\mu}m$) and meteorological parameters, such as relative humidity, wind speed and visual range, while the correlation between the number concentration of small particles ($0.5{\sim}2.23{\mu}m$) and relative humidity showed a positive value. This trend was inversed for the case of wind speed: aerosol number concentration showed a small decreasing tendency with increasing wind speed for small particles but the high wind speed in winter season increased coarse particle concentration. Finally, Particles most efficient in light extinction were found to be at the size of about $0.5{\sim}1{\mu}m$.

19세기 베를린 '미츠카제리네' 블록하우징에 관한 고찰 - Hackesche Hoefe 리모델링을 중심으로 -

이명주,Lee, Myoung-Ju 한국건축역사학회 2004 건축역사연구 Vol.13 No.1

The $18^{th}$ century's Industrial Revolution brought about influx of commerce, industry, and agricultural population into the urban areas, entering the $19^{th}$ century. Quality of rural communities declined, and the exploding population in the cities gave rise to various problems. 'Mietskaserne' Blockhousing was constructed by the 'Hobrechtplan', but led to social problems such as poor living conditions, insufficient sunshine due to overcrowding, slums due to incomplete urban infrastructure, epidemics, and so on. Starting in the early $20^{th}$ century, Berlin has driven forward a remodeling plan under the motto of 'critical reconstruction (kritische Rekonstruktion)'. It is performed in the place, which represents the vicissitudinous history of Berlin with site plans coexisting past with presence, using modern vocabulary of architectural forms. Reconstructing a city is a process which not only raises the economic value of each building consisting a city, but also a redevelopment process that brings out cultural value of an era. When a new era emerges buildings get reconstructed or rebuilt, and thereby form the identity of a city by reflecting its society, culture, politics, economy, and history. Old German architecture were not destroyed or rebuilt recklessly just by the fact that they are functionally or aesthetically outdated. Each building is treated as precious cultural heritage reflecting the history. This is how Berlin is being transformed today.

저온 분사 공정으로 제조된 Ti 코팅층의 미세조직 및 물성에 미치는 송급 가스의 영향

이명주,김형준,오익현,이기안,Lee, Myeong-Ju,Kim, Hyung-Jun,Oh, Ik-Hyun,Lee, Kee-Ahn 한국분말야금학회 2013 한국분말재료학회지 (KPMI) Vol.20 No.1

The effect of carrier gases (He, $N_2$) on the properties of Ti coating layers were investigated to manufacture high-density Ti coating layers. Cold spray coating layers manufactured using He gas had denser and more homogenous structures than those using $N_2$ gas. The He gas coating layers showed porosity value of 0.02% and hardness value of Hv 229.1, indicating more excellent properties than the porosity and hardness of $N_2$ gas coating layers. Bond strengths were examined, and coating layers manufactured using He recorded a value of 74.3 MPa; those manufactured using $N_2$ gas had a value of 64.6 MPa. The aforementioned results were associated with the fact that, when coating layers were manufactured using He gas, the powder could be easily deposited because of its high particle impact velocity. When Ti coating layers were manufactured by the cold spray process, He carrier gas was more suitable than $N_2$ gas for manufacturing excellent coating layers.

회원작품 - 어썸 845

이명주,Lee, Myeongju 대한건축사협회 2018 建築士 Vol.587 No.-

Mode S를 이용한 지역 다변측정감시 시스템의 기준감시트랜스폰더

이명주(Myung-Joo Lee),강승배(Seungbae Kang),조규인(Gyuin Cho),이용석(Yongsuk Lee),이광원(Kwangweon Lee) 한국정보과학회 2015 한국정보과학회 학술발표논문집 Vol.2015 No.12

건축물에너지절약요소기술 적용에 따른 단독주택 에너지 요구량 절감률 변화에 관한 연구

이명주(Lee Myoung-Ju),김원석(Kim Won-Seok),이우주(Lee Woo-Joo),이원택(Lee Won-Taek) 대한건축학회 2012 대한건축학회논문집 Vol.28 No.5

This study is to compare and analyze ‘the Korean energy saving design standard in building’ with ‘the passivehouse standard of PHI’ in Germany in using of energy efficient factors which have an effect on reducing energy demand in building. We used following architectural design method for energy efficiency : direction, south oriented window-wall ratio, insulation, glazing, frame, heat recovery, external blind, airtightness, thermal mass, ect. We set a base model and analyzed annual energy demand, heating&cooling demand through energy-simulation according to each standard above by energy efficient elements. Consequently, ‘the passivehouse standard of PHI’ is more effective on reducing energy use than ‘the Korean energy saving design standard in building’. Also, this study found energy efficiency rate for heating demand by elements and it is as in the following : Window(37.92%) > Heat recovery(27.87%) > Insulation(25.23%) > Airtightness(5.49%) > Internal/External Insulation(4.42%) > Thermal mass(3.99%) > South oriented window-wall ratio(0.29%) > External blind(0%). And we found that the external blind(56.2%) takes a key role in reducing cooling demand. Through this study, we discovered energy reducing rate by energy efficiency factors in building and with this result, we are expecting to make energy efficiency design standard for the improvement of the high energy-use detached house.

지역 다변측정감시 시스템의 다중 기준감시트랜스폰더를 위한 XML기반 ADIF 설계

이명주(Myung-Joo Lee),윤한식(Hansik Yoon),강승배(Seungbae Kang),이용석(Yongsuk Lee),이광원(Kwangweon Lee) 한국정보과학회 2017 한국정보과학회 학술발표논문집 Vol.2017 No.06