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고성능액체크로마토그라피/자외부검출기를 이용한 조제분유 중 수용성 비타민의 동시분리 정량분석
박성원,이득신,손성완,김재훈,조남인,김옥경 한국수의공중보건학회 2001 예방수의학회지 Vol.25 No.1
An ion pair liquid chromatographic method is described for the simultaneous determination of water-soluble vitamins C, B1, B2, B6 and nicotinamide in dried milk infant formulations. Commercial infant formulations and water-soluble vitamin spiked samples of the formulations were extracted with distilled water consisting of 5mM of hexane sulfonic acid sodium salt, 2% meta[hosphoric acid and 1% acetic acid and filtrated. And the subsequent analysis of the filtrate was performed by liquid chromatography. The filtrate contained water-soluble vitamins were free from interfering compounds when analyzed by liquid chromatography with UV detection (variable wavelength, 270 & 290nm). Gradient elution was required to elute vitamin C, B1, B2, B6 and nicotinamide from a C18 reversed phase column using the mobile phase consisting of 0 to 60% methanol contained 1% acetic acid and the counter ion, 5mM hexane sulfonic acid. Correlation coefficients of standards curves for individual vitamin from standard solution were linear (from 0.9985 to 0.9999) with average percentage recoveries from 85.0 to 116.9 for the concentration range (1.0∼400μg/g) examined.
LTLT 및 HTST처리 살균우유의 보관방법별 품질변화에 관한 연구
정석찬,김계희,정명은,김성일,변성근,이득신,정승교,박성원,전기석,이길홍,조남인,이홍길,김옥경 한국수의공중보건학회 2001 예방수의학회지 Vol.25 No.4
This study was conducted to investigate the quality changes of the 145 LTLT (law temperature long time; 63℃ for 30 mins) and 145 HTST (high temperature short time; 72℃ for 15s) treated milk samples by storage conditions for 30 days from September to October 2000. Raw milk samples collected from storage tanks in 2 milk plants were showed 1.3∼4.0×10^5 CFU/ml in standard plate count, and normal level in acidity, specific gravity, and components of milk. Preservatives, antibiotics, sulfonamides and available chloride were not detected in both raw and heat treated milk samples obtained from 2 plants. The bacterial counts in the LTLT and HTST pasteurized milk samples were about 4.0×103 and 1.5×10^1 CFU/ml at the production day, respectively. The bacterial counts in the samples were rapidly increased to more than 10^7 CFU/ml at room temperature (12℃∼30℃) for 3 days, but were kept less than 2×10^4 CFU/ ml at refrigerator (10℃) for 7 days of storage. The sensory quality and acidity of the milk samples were gradually changed in proportion to bacterial counts, but the specific gravity was not significantly changed for 30 days. No significant correlation was found in keeping quality of the milk between dark and light exposure at room temperature for 30 days. The compositions of fat, solid-not fat, protein and lactose in milk were not significantly changed according to the storage conditions for 30 days. The LTLT and HTST pasteurized milk should be sanitarily handled, kept and transported under refrigerated condition (below 7℃) in order to supply wholesome milk to consumers.