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MK-801 투여에 의한 몰핀의존성랫드 뇌선조체중 도파민신경전달물질의 변화
이선희(Sun Hee Lee),신대섭(Dae Sup Shin),이덕주(Duck Joo Rhie),유영아(Young A Yoo),류승렬(Seung Rel Ryu),김대병(Dai Byung Kim),이종권(Jong Kwon Lee),김부영(Pu Young Kim) 한국응용약물학회 1998 Biomolecules & Therapeutics(구 응용약물학회지) Vol.6 No.1
The roles of dopamine(DA) and N-methyl-D-aspartate(NMDA) system in the development and expression of morphine dependence were investigated by monitoring the concentrations of extracellular DA and its metabolites by in vivo microdialysis and simultaneous observation of behavioral changes in morphinedependent rats. Extracellular DA level in caudate putamen of morphine-dependent rat was decreased and the concentrations of its metabolites, dihydroxy phenylacetic acid(DOPAC) and homovanillic acid(HVA), were increased during naloxone-precipitated withdrawal. DA contents were recovered to normal levels by pretreatment of MK-801, a noncompetitive NMDA receptor antagonist, which may explain the mechanism of diminishing effect of MK-801 on withdrawal symptoms in morphine-dependent rats. MK-801(0.3 mg/kg, i.p.) induced the untoward harmful neurological signs such as ataxia and severe rotations, which may be produced by hyperactivation of dopaminergic system. These results suggest that MK-801 may inhibit the expression of morphine dependence by altering the dopamine release.
김명석(Kim, Myung-Suk),조양혁(Jo, Yang-Hyeok),윤신희(Yoon, Shin-Hee),한상준(Hahn, Sang-June),이덕주(Rhie, Duck-Joo),김정진(Kim, Chung-Chin) 대한생리학회 1989 대한생리학회지 Vol.23 No.2
Sixty-seven conscious rats prepared with chronic gastric fistula were studied to examine the effect of vagotomy on gastric secretory responses to medial amygdaloid stimulation. Gastric acid output was significantly increased by electrical stimulation of the medial amygdaloid nucleus, and the increases in acid secretion were completely eliminated by vagotomy. However, in rats subjected to stimulation of the medial amygdaloid nucleus plus vagotomy, acid output was almost same as that in only vagotomized rats. And vagotomy itself decreased the acid secretion significantly. These results indicate that the influence of electrical stimulaton of the medial amygdaloid nucleus on acid secretion is carried largely via vagus nerves. And also, without electrical stimulation of medial amygdaloid nucleus, acid secretion is controlled by way of vagus in rats. Plasma gastrin concentrations were measured following stimulation of the medial amygdaloid nucleus. Plasma levels of gastrin were not significantly altered by stimulation of the medial amygdaloid nucleus with or without vagotomy. It is therefore inferred from the above results that the facilitatory influence of the medial amygdaloid nucleus on the gastric acid secretion is mediated chiefly via vagal activity and that gastrin is not responsible for the increase in acid secretion in this process.
실험연구 : 일차 배양된 흰쥐 해마 신경세포에서 Fluoxetine의 생체 내 대사산물인 Norfluoxetine에 의한 전압의존성 K(+) 이온통로의 억제
한태형 ( Tae Hyung Han ),최진성 ( Jin Sung Choi ),곽인숙 ( In Suk Kwak ),최유준 ( You Jun Choi ),김명준 ( Myung Jun Kim ),민도식 ( Do Sik Min ),이덕주 ( Duck Joo Rhie ),윤신희 ( Shin Hee Yoon ),조양혁 ( Yang Hyeok Jo ),김명석 ( My 대한마취과학회 2003 Korean Journal of Anesthesiology Vol.45 No.3
고양이 위 평활근에서 cyclic nucleotide의 자발적 생성과 작용
김명석,이덕주,조양혁,윤신희,한상준,심상수,전중선,백혜정 대한소화기학회 1998 대한소화기학회지 Vol.30 No.6
Background/Aims: The inhibitory neurotransmitter increased the level of cellular cyclic nucleotides and induced smooth muscle relaxation. The aim of this study was to investigate the spontaneous foimation and action of cyclic nucleotides in feline gastric smooth muscle. Methods: Isolated muscle strips were obtained from the fundus of the stomach to measure isometric contraction and muscle cells were prepared with collagenase and papain to measure the level of cyclic nucleotides were also measured by radioimmunoassay. Results: 3-Isobutyl-1-methylxanthine (IBMX) inhibited acetylcholine-induced contraction in a dose dependent manner. Both SQ22536 and methylene blue completely blocked the inhibitory effect of IBMX on acetylcholine-induced contraction. IBMX increased the levels of cAMP and cGMP in muscle cells. The increase in cAMP and cGMP levels by IBMX was inhibited by SQ22536 and methylene blue, respectively. Inhibitory neurotransmitters, such as vasoactive intestinal polypeptide, nitric oxide donor(sodium nitroprusside) and isoprenaline, significantly inhibited acetylcholine-induced contraction via an increase of cyclic nucleotides in the muscle cells. However, antagonists or inhibiiors for inhibitory neurotransmitters did not have an any influence on the inhibitory effect of IBMX. Conclusions: It appears that the formation of cyclic nucleotides including cAMP and cGMP occurs spontaneously in the gastric muscle cells. Therefore, the importance of phosphodiesterase action as well as the formation of the cyclic nucleotides should be considered in evaluating the mechanism of feline gastric smooth muscle relaxation.
고양이 위 평활근 수축에 대한 Vanadate 와 Pervanadate 의 효과
김명석,이덕주,심혜선,조양혁,심상수,최효봉,윤신희,한상준 대한소화기학회 1999 대한소화기학회지 Vol.33 No.1
Background/Aims: Activated mammalian cells produce the reactive oxygen species from oxygen and they convert vanadate into pervanadate. The aim of the present study was to compare the contactile mechanisms of vanadate and pervanadate in the gastric smooth muscle of cat. Methods: Muscle strips were isolated from the fundus of cat stomach to measure isometric contraction. Muscle cells were prepared to measure the activity of protein kinase C (PKC) and the level of protein tyrosine phosphorylation. Results: Both vanadate and pervanadate caused contractions of smooth muscle and the contraction induced by pervanadate was greater than that induced by vanadate. These contractions depended on the extracellular Ca2+ and the influx of extracellular Ca2+ occurred through voltage-dependent Ca2+ channel. Protein kinase antagonist inhibited the vanadate-induced contraction. However, the inhibitory effect of H-7 was smaller in pervanadate-induced contraction. Both vanadate and pervanadate had no effect on the activity of phospholipase C, but pervanadate significantly increased protein tyrosine phosphorylation. Conclusions: The contraction induced by vanadate or pervanadate may depend on the influx of extracellular Ca2+ via voltage-dependent Ca2+ channel Vanadate seems to be more dependent on protein kinase C. The difference of contraction mechanism by these elements may be related to the differences in the level of protein tyrosine phosphorylation (Kor J Gastroenterol 1999;33:1 - 10)