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인삼Saponin의 Aspergilius parasiticus R-716의 생육 및 Aflatoxin생성에 미치는 영향
이광승,장진규 고려인삼학회 1986 Journal of Ginseng Research Vol.10 No.1
The potential effects of ginseng saponin on the growth, aflatoxin production, and physicochemical characteristics of Aspergillus parasiticus R-716 were investigated and the results obtained were summarized as follows. The pH values of culture filtrate were increased with an increase of addition amount of saponins, the amount of mycelium was increased up to l19% by the addition of 0.01% protopanaxatriol saponin (triol). Amount of aflatoxin was increased in proportion as the bright yellow color of chloroform extract of culture filtrate was intensified. There was no difference in sporulation by the addition of 0.02% saponins, however, the sporulation was gradually decreased as the addition concentration of saponins increased. Aflatoxin production was reduced to the level of 8% by the addition of crude saponin, but production of aflatoxin B1 and B2 were inhibited by 56% and 8% with the addition of 0.5% pure saponin. The production of aflatoxin B. was increased by the addition of 0.5% trios saponin, and by the addition of 0.02% biol saponin, aflatoxin G, production reached to the maximum and thereafter it was decreased. 인삼 saponin의 첨가가 Aspergillus parasiticus R-716 공시균의 PH, 균체량의 변화, chloroform 추출액의 색상, 포자형성능, 그리고 saponin의 첨가량에 따른 aflatoxin의 생성 및 억제효과등을 조사한 결과를 다음과 같다. 1. 공시균의 배지인 sucrose low salt의 PH변화는 crude saponin(CS)의 경우 PH3인 0.5% 첨가구에서 가장 낮은 aflatoxin 생성량을 보였으며, PH 2.2인 protopanaxatriol(PPT) 0.05% 첨가구에서 가장 많은 생성량을 보였다. 2. 인삼 saponin첨가량에 따른 균체량의 변화는 PPT 0.01% 첨가구에서 대조구에 비하여 119%까지 증가하였으나 다른 saponin의 첨가농도가 증가할 수록 감소하였고 CS 0.5% 첨가구에서 대조구에 비하여 25.8%의 감소율을 보였다. 3. aflatoxin의 chloroforn 추출시 생성된 색상은 밝은 황색일수록 aflatoxin의 생성량이 많았다. 4. 포자형성은 각 인삼 saponin 농도가 0.02%까지는 변화가 없었으나 0.05%첨가구부터 형성이 줄어들어 0.5% 첨가구에서는 형성되지 않았다. 5. aflatoxin총생성량은 PPD와 PPT 0.005%~0.02% 첨가구에서는 105%까지 증가하였으나 그 이상의 농도에서는 감소를 보였으며, CS의 0.5% 첨가구에서는 91.7%의 높은 억제효과를 보였고 B1, B2, G1 및 G2에 대햐여는 90%이상의 억제효과를 나타내었다. PS에 대하여는 0.5% 첨가구에서 B1에 55.9%의 억제효과가 있는 반면에 B2는 8.1%의 낮은 억제효과밖에 없었고, PPT 0.005% 및 0.5% 첨가구는 오히려 B2가 증가하였다. PPD에 대하여는 0.005%~0.02% 첨가구에서는 G1의 경우 오히려 증가하였다.
30㎚~3000㎚ 광범위 직경 입자의 공기역학적 집속에 대한 수치해석
이광승,이동근 한국입자에어로졸학회 2011 Particle and Aerosol Research Vol.7 No.4
Previous designs of conventional aerodynamic lenses have a limitation of narrow range of focusable particle size, e.g. 30 to 300nm or 3 to 30nm. To enlarge the focusable size range to 30‐3000nm, it is necessary to avoid a significant loss of particles larger than 300nm inside the lenses. From numerical simulations on size‐resolved particle trajectories, we confirmed that the traveling losses of such large particles could be avoided only when the radial position of particles approaching the orifice lens was near the lens axis. Hence, we designed the lens system consisting of a converging‐diverging nozzle and 7 orifices to fulfill the requirement. In particular, the orifices were aligned in a way that their diameters were descending and ascending to the downstream. As a result, 30‐2800nm particles can be focused to the particle beam of 0.2mm or less in radius with above 85% transmission efficiency. Even 10μm particles can be focused with 74% of transmission efficiency.
수축-발산형 공기역학렌즈를 이용한 초미세 나노 입자의 집속
이광승(Kwang-Seung Lee),김송길(Song Kil Kim),이동근(Dong Geun Lee) 대한기계학회 2008 大韓機械學會論文集B Vol.32 No.8
An orifice type of aerodynamic lens is generally used to focus nanoparticles. However, it is impossible to focus particles smaller than 10㎚ in air due to flow instability of fluid in a lens. In this study, we propose a new converging-diverging type of the aerodynamic lens capable of focusing particles of 5-50㎚ in air. Designing factors of the lens configurations is also extracted and explained in detail through a numerical simulation. It was demonstrated that the aerosols are delivered from the entrance to the downstream of the lens system with 90% transmission efficiency. The final beam diameters are shown to be more or less 1㎜ in the range of particle size.