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The presence of CD8+ invariant NKT cells in mice
Hyunji Lee,홍창완,신정훈,Soohwan Oh,정선도,박윤경,홍석만,이갑열,박세호 생화학분자생물학회 2009 Experimental and molecular medicine Vol.41 No.12
Invariant natural killer T (iNKT) cells develop in the thymusupon recognition of CD1d expressed on developingthymocytes. Although CD4 and CD8 coreceptorsare not directly involved in the interaction betweenCD1d and the T cell receptors (TCRs) of iNKT cells, aconspicuous lack of CD8+ iNKT cells in mice raised thequestion of whether CD8+ iNKT cells are excluded dueto negative selection during their thymic development,or if there is no lineage commitment for the developmentof murine CD8+ iNKT cells. To address this question,we analyzed iNKT cell-specific TCR Vα14+ transgenicmice, where the Vα14 transgene forces the generationof iNKT cells. This allows detailed study of theiNKT cell repertoire. We were able to identify CD8+ iNKTcells which respond to the NKT cell-specific glycolipidligand α-galactosylceramide. Unlike conventionaliNKT cells, CD8+ iNKT cells produce predominantlyIFN-γ but not IL-4 upon antigen stimulation. We alsoconfirmed the presence of CD8+ iNKT cells in wild typemice. Our results suggest that CD8+ NKT cells do existin mice, although their population size is quite small. Their Th1-skewed phenotype might explain why thepopulation size of this subtype needs to be controlledtightly.
정선도,박윤경,신정훈,Hyunji Lee,Soo-Young Kim,이갑열,박세호 생화학분자생물학회 2010 Experimental and molecular medicine Vol.42 No.8
TGF-β-induced tolerogenic-antigen presenting cells (Tol-APCs) could induce suppression of autoimmune diseases such as collagen-induced arthritis (CIA) and allergic asthma. In contrast, many studies have shown that NKT cells are involved in the pathogenesis of Th1-mediated autoimmune joint inflammation and Th2-mediated allergic pulmonary inflammation. In this study, we investigated the effect of CD1d-restricted NKT cells in the Tol-APCs-mediated suppression of autoimmune disease using a murine CIA model. When CIA-induced mice were treated with Tol-APCs obtained from CD1d+/- or CD1d-/- mice, unlike CD1d+/-APCs, CD1d-/- Tol-APCs failed to suppress CIA. More specifically, CD1d-/- Tol-APCs failed to suppress the production of inflammatory cytokines and the induction of Th2 responses by antigen-specific CD4 T cells both in vitro and in vivo. Our results demonstrate that the presence of CD1d-restricted NKT cells is critical for the induction of Tol-APCs-mediated suppression of CIA.
세그멘트 폴리우레탄 우레아에 기초한 블렌드계 고체전해질의 분자운동성과 이온 전도성
이상걸,이갑열,조남주 부산대학교 생산기술연구소 2000 生産技術硏究所論文集 Vol.59 No.-
순수 고체 전해질과 겔형 고분자 전해질의 장점을 취합한 새로운 형태의 블렌드계 고체 전해질을 제조할 목적으로, 현재 유기 가소제로 널리 쓰이는 ethylene carbonate(EC), propylene carbonate(PC) 대신 실온에서 휘발성이 낮은 저분자량의 polyethylene g1ycol(PEG) 및 polyethylene glycol dimethylether(PEGDME)를 사용하였다. 또한, 고분자 매트릭스로는 지지체 역할 뿐만 아니라 염과의 복합체를 형성할 수 있도록 soft segment에 PEO/PPO 공중합체를, hard segment urea기를 도입한 세그멘트 폴리우레탄 우레아(segmented polyurethane urea, SPUU)를 사용하였다. 제조된 SPUU팀에 휘발성이 낮은 저분자량의 PEC 및 PEGDME와 리튬염을 부가하여 solution casting법으로 블렌드계 고체 전해질을 제조한 다음 교류 임피던스법으로 이온 전도도를 측정하였다. 이때 이온 전도도는 PEO 및 PEGDME의 분자량 및 함량에 의존함을 관찰할 수 있었다. In order to prepare a new blend-type electrolyte possessing the merits of both an intrinsic polymer electrolyte and gel-electrolyte, we report on a class of nonvolatile (dry) polymer electrolyte, prepared by plasticizing a segmented polyurethaneurea (SPUU) with solution of lithium salt in oligomeric poly(ethylene glycol) (PEG) and poly(ethylene glycol) dimethyl ethers (PEGDME). SPUU having PEO/PPO copo1ymer in soft segment and urethanerurea group in hard segment was used in order to prepare a mechanically stable blend-type electrolyte for for improving the affinity between PEC or PEGDME and matrix. and the ionic conductivity was measured by AC impedance spectroscopy. As a result, it was observed that the ionic conductivity depended on the molecular weight and contents of PEC and PEGDME.