Effect of High-tyrosine Diet on Brain Norepinephrine Metabolism in Immobilization-Stressed Rats

Yoon Hae Seong(윤혜성),Kim Harriet(김해리) 韓國營養學會 1993 Journal of Nutrition and Health Vol.26 No.7

이온크로마토그래프를 이용한 조제유류 및 영아용·성장기용 조제식 중 콜린 함량 분석법 연구

황경미,함현숙,이화정,강윤정,윤혜성,홍진환,이현영,김천회,오금순,Hwang, Kyung Mi,Ham, Hyeon Suk,Lee, Hwa Jung,Kang, Yoon Jung,Yoon, Hae Seong,Hong, Jin Hwan,Lee, Hyoun Young,Kim, Cheon Hoe,Oh, Keum Soon 한국식품위생안전성학회 2017 한국식품위생안전성학회지 Vol.32 No.5

This study was conducted to establish the analysis method for the contents of choline in infant formulas and follow-up formulas by ion chromatograph (IC). To optimize the method, we compared several conditions for extraction, purification and instrumental measurement using spiked samples and certified reference material (CRM; NIST SRM 1849a) as test materials. IC method for choline was established using Ion Pac CG column and 18 mM $H_2SO_4$ mobile phase. The parameters of validation were specificity, linearity, LOD, LOQ, recovery, accuracy, precision and repeatability. The specificity was confirmed by the retention time and the linearity, $R_2$ was over 0.999 in range of 0.5~10 mg/L. The detection limit and quantification limit were 0.14, 0.43 mg/L. The accuracy and precision of this method using CRM were 95%, 2.1% respectively. Optimized methods were applied in sample analysis to verify the reliability. All the tested products were acceptable contents of choline compared with component specification for nutrition labeling. The standard operating procedures were prepared for choline to provide experimental information and to strengthen the management of nutrient in infant formula and follow-up formula. 본 연구는 "축산물의 가공기준 및 성분규격"에 기준규격은 설정되어 있지 않으나, 국제 기준과의 조화를 위해 콜린의 분석법을 마련하고자 수행하였다. 조제유류에 함유된 콜린 함량 분석을 위해 IC를 이용한 분석법을 확립하고 시중에 유통 중인 제품을 대상으로 적용성을 검토하였다. 콜린 표준품을 이용하여 IC를 이용한 기기분석조건을 확립하고 시료중의 콜린을 추출하여 분석하였다. 분석법 검증은 특이성, 직선성, 검출한계 및 정량한계, 정확성, 정밀성에 대해 수행되었다. 0.5~10 mg/L의 농도범위에서 $R^2=0.999$ 이상의 우수한 직선성을 확인할 수 있었으며, LOD와 LOQ는 각각 0.14, 0.43 mg/L였다. CRM (NIST SRM 1849a) 및 표준물질 첨가법을 이용하여 정확성을 검토하였으며, CRM에서 95%, 조제분유에서 90~91%, 조제우유에서 81~98%의 회수율을 확인할 수 있었다. 정밀성을 검토한 결과 시료 채취량에 따른 반복성은 RSD값이 조제분유 0.4~2.0%, 조제우유 0.5~1.5%, 영아용조제식 0.6~1.0%, 성장기용조제식 0.8~2.7%,로 확인하였으며, 실험일자에 따른 재현성은 조제분유 3.1%, 조제우유 2.5%, 영아용조제식 4.8%, 성장기용조제식 2.7%로 확인하였다. 본 연구에서 확립된 분석법을 적용하여 조제분유 11건, 조제우유 2건, 성장기용조제분유 9건, 영아용조제식 2건, 성장기용조제식 8건 등 국내 유통 중인 조제유류 및 영아용 성장기용조제식 등 32건에 대해 적용성 검토를 실시한 결과 전체 시료에서 분석이 용이하였으며, 모두 표시기준에 적합함을 확인하였다.

생약 복용에 따른 아플라톡신 B₁의 인체모니터링 연구

이진희,류희영,김현경,김도정,이영주,정수희,장동덕,김형수,홍연표,윤혜성,Lee, Jin-Hee,Ryu, Heui-Young,Kim, Hyun-Kyung,Kim, Do-Jung,Lee, Young-Joo,Jung, Su-Hee,Jang, Dong-Deuk,Kim, Hyung-Su,Hong, Yeon-Pyo,Yoon, Hae-Seong 한국환경보건학회 2010 한국환경보건학회지 Vol.36 No.2

Aflatoxin $B_1$, a known human carcinogen, is the member of aflatoxin subfamily that is most frequently found in contaminated foods. Epidemiological studies have suggested that aflatoxins may be associated with human liver cancer and acute hepatitis. Recently it was reported that the traditional medical herbs sold in domestic markets are contaminated with aflatoxins. Long-term administration of these contaminated medicines could result in adverse health effects. Therefore, it is important to evaluate the levels of exposure to aflatoxin in people who ingest traditional herbal medicines. Blood samples were collected, before and after the herbal medicine intake, from 151 subjects who visited the hospital. The metabolite of aflatoxin $B_1$ in blood, aflatoxin $B_1$-albumin (aflatoxin $B_1$-lysine), is reportedly an appropriate internal exposure indicator, and its levels in the collected bloods were therefore analyzed using a liquid chromatography-mass spectrometry. The analytical method of aflatoxin $B_1$-lysine in blood was firstly optimized in Korea and the levels were detected below quantification limits (2 pg/mg albumin) in this study population. Consequently, the exposure levels of aflatoxin $B_1$ by ingestion of herbal medicines were low but it is important to monitor routinely due to the possibility of risk on the aflatoxin exposure.

2010년~2014년 국민건강영양조사 자료를 이용한 성인의 나트륨 섭취와 비만과의 관련성

천세영(Cheon, Se Young),왕혜원(Wang, Hye Won),이화정(Lee, Hwa Jung),황경미(Hwang, Kyung Mi),윤혜성(Yoon, Hae Seong),강윤정(Kang, Yoon Jung) 한국영양학회 2017 Journal of Nutrition and Health Vol.50 No.1

본 연구는 국가적으로 시행된 대규모 국민건강영양조사 자료를 이용하여 나트륨 섭취량과 비만과의 연관성을 알아보기 위하여 나트륨 섭취량을 5그룹 (2,000 mg 미만, 2,000 mg 이상~4,000 mg 미만, 4,000 mg 이상~6,000 mg 미만, 6,000 mg 이상~8,000 mg 미만, 8,000 mg 이상)으로 분류하여 비만과의 연관성을 분석하였다. 연구대상자의 성별에 따른 일반적인 특징에서 신장과 체중, 체질량지수(BMI), 총 에너지섭취량은 남성이 여성보다 유의적으로 높게 확인되었고, 질병관련 변수에서 비만, 고혈압, 당뇨병, 뇌졸중 유병률 또한 남성이 여성보다 유의적으로 높은 값을 보였다. 성별에 따른 교육수준 (p < 0.001), 흡연여부 (p < 0.001), 음주여부 (p < 0.001)에서도 유의한 차이가 확인되었다. 나트륨 섭취량에 따른 신체계측치 및 건강관련 습관에 대해 분석한 결과, 전체 대상자에서 나트륨 섭취량이 증가할수록 신장, 체중, BMI, 총에너지섭취량이 유의적으로 높아지는 것을 확인할 수 있었으며, 남성과 여성에서도 같은 결과를 보였다. 비만, 고콜레스테롤혈증과 고중성지방혈증 유병률은 나트륨 섭취량이 높아짐에 따라 증가하는 경향을 보였고, 남성에서도 유사한 결과가 나타났다. 나트륨 섭취량에 따른 교육수준, 걷기실천율, 흡연여부, 음주여부에서도 유의한 차이가 확인되었다. 성별에 따른 나트륨 섭취량이 비만 유병에 미치는 영향을 교란변수 보정 전후로 비교하여 분석한 결과, 전체 대상자에서는 나트륨섭취량이 증가할수록 2,000 mg 미만 섭취자 대비 비만의 오즈비가 증가하였으며, 남성의 경우 4,000 mg 이상 섭취자부터 유의한 OR값을 보였다. 성별, 나이, 연도, 에너지밀도, 교육수준, 흡연여부, 음주여부, 만성질환 유병여부, 신체활동을 보정하였을 때, 4,000~6,000 mg 섭취그룹 및 8,000 mg 이상 섭취그룹이 2,000 mg 미만 섭취자보다 비만 유병위험이 높게 나타났으며, 남성은 8,000 mg 이상 섭취그룹에서, 여성은 4,000~6,000 mg 섭취자 그룹에서 각각 비만의 유의한 오즈비를 나타내었다. Purpose: Excess sodium intake may contribute to the etiology of hypertension and cardiovascular disease risk. World Health Organization (WHO) recommends a daily sodium intake of less than 2 g. The aim of this study was to estimate the association of sodium intake with obesity in Korean adults. Methods: This study used Dietary intake and Health data on 22,321 subjects aged 30 years and over from the Korea National Health and Nutrition Examination Survey (KNHANES)2010~2014. Information on dietary intake was obtained by the one day 24-hour recall method in KNHANES, and sodium intake was classified into five groups (< 2,000 mg, 2,000~4,000 mg, 4,000~6,000 mg, 6,000~8,000 mg, ≥ 8,000 mg). Obesity was defined as having a body mass index (BMI) higher than 25 kg/㎡. Intake of sodium and obesity status were analyzed by logistic regression with SPSS Statistics 23. Results: Men tended to have a higher sodium intake than women (p < 0.001). After adjusting for age, sex, year, daily energy intake, education level, smoking status, drinking status, physical activity, and chronic diseases and comparing the highest sodium intake group (≥ 8,000 mg) with the lowest intake group (< 2,000 mg), the OR of obesity was 1.351 (95% CI: 1.032~1.767) in men. The OR of obesity in the sodium intake group (4,000~6,000 mg) was 1.232 (95% CI: 1.063~1.427) in women. Conclusion: Our findings suggest an independent relationship between sodium intake and as increased risk of obesity in Korean adults, implying the necessity for future research on low-sodium diet intervention in relation to obesity.

연구보문 : 수질환경 ; 고성능액체크로마토그래피-유도결합플라즈마 질량분석기를 이용한 어류 중 메틸수은 분석법 확립

유경열 ( Kyung Yoal Yoo ),반경녀 ( Kyeong Nyeo Bahn ),김은정 ( Eun Jung Kim ),김양선 ( Yang Sun Kim ),명정은 ( Jyong Eun Myung ),윤혜성 ( Hae Seong Yoon ),김미혜 ( Mee Hye Kim ) 한국환경농학회 2011 한국환경농학회지 Vol.30 No.3

BACKGROUND: Methylmercury is analyzed by HPLC-ICP/MS because of the simplicity for sample preparation and interference. However, most of the pre-treatment methods for methylmercury need a further pH adjustment of the extracted solution and removal of organic matter for HPLC. The purpose of this study was to establish a rapid and accurate analytical method for determination of methylmercury in fish by using HPLC-ICP/MS. METHOD AND RESULTS: We conducted an experiment for pre-treatment and instrument conditions and analytical method verification. Pre-treatment condition was established with aqueous 1% L-cysteine·HCl and heated at 60℃ in microwave for 20 min. Methylmercury in 50 μL of filtered extract was separated by a C18 column and aqueous 0.1% L-cysteine·HCl + 0.1% L-cysteine mobile phase at 25℃. The presence of cysteine in mobile phase and sample solution was essential to eliminate adsorption, peak tailing and memory effect problems. Correlation coefficient(r2) for the linearity was 0.9998. The limits of detection and quantitation for this method were 0.15 and 0.45 μg/kg respectively. CONCLUSION: Result for analytical method verification, accuracy and repeatability of the analytes were in good agreement with the certified reference materials values of methylmercury at a 95% confidence level. The advantage of the established method is that the extracted solution can be directly injected into the HPLC column without additional processes and the memory effect of mercury in the ICP-MS can be eliminated.

피부감작성 동물대체시험법인 ARE-Nrf2 루시퍼라아제 LuSens 시험법(OECD TG 442D)의 국내 확립 연구

홍미혜 ( Mi Hye Hong ),조인숙 ( In-suk Joe ),방서영 ( Seo Young Bang ),이정선 ( Jung-sun Yi ),김광진 ( Kwang Jin Kim ),윤혜성 ( Hae Seong Yoon ),김태성 ( Tae Sung Kim ) 한국동물실험대체법학회 2021 동물실험대체법학회지 Vol.15 No.1

This study aimed to establish the LuSens test method for identification of skin sensitisers in our laboratory and to facilitate the domestic use of the method. We utilized 10 recommended proficiency substances in OECD TG 442D consisting of 6 skin sensitisers (UN GHS category 1A and 1B: Eugenol, Cinnamyl alcohol, 2-Mercaptobenzo-thiazole, 4-Methylaminophenol sulfate, Methyl dibromo glutaronitrile and 2,4-Dinitro-chlorobenzene) and 4 non-sensitisers (No category: Salicylic acid, Glycerol, Isopropanol and Sulfanilamide). We measured the activity of luciferase induced by the test substances based on the CV₇₅ that was determined by cytotoxicity dose-finding test. While the maximal luciferase fold induction values for each skin non-sensitisers ranged from 0.95 to 1.28, those for each skin sensitisers ranged from 1.96 to 5.66. We predicted sensitivity of the test substances on the basis of the luciferase fold induction values. Our results were within the range of acceptance criteria and accord with in vivo and in vitro references in OECD TG 442D. We found that the LuSens test method correctly identified proficiency substances into sensitisers and non-sensitisers. Therefore, the results obtained from the proficiency test demonstrated that we successfully introduced the LuSens test method in our laboratory. Furthermore, we have prepared a new in vitro skin sensitization test (ARE-Nrf2 luciferase LuSens) guideline for the safety evaluation of cosmetics and contributed to the dissemination of the method via technical transfer in Korea.