흰쥐의 복강비만세포에서 ATP와 Compound 48/87에 의한 Histamine유리에 미치는 Econazole의 영향

장용운(Yong Un Jang),이윤혜(Yun Hye Lee),이승준(Seung June Lee),서무현(Moo Hyun Seo),윤정이(Jeong Yi Yoon),김창종(Chang Jong Kim),심상수(Sang Soo Sim) 대한약학회 2001 약학회지 Vol.45 No.3

3 investigate the different mechanism between ATP and compound 48/80 (C48/80)-induced histamine release, we observed effects of calcium antagonists in histamine release of rat peritoneal mast cells.Verapamil and diltiazgem (voltage-dependent calcium channel blocker) and TMB-8 (a blocker of intracellular calcium release) significantly inhibited ATP-induced histamine release, but did not inhibit C48/80 induced histamine release. Econazole (a blocker of receptor-operated calcium channel) dose-dependently inhibited both and C48/80-inducted histamine release,but inhibitory effect of econarole in ATP-induced histamine release was more potent than that in C48/80-induced histamine. EGTA dote-dependently inhibited ATP and C48/80-induced histamine release, and C48/80-induced histamine release was slightly inhibited by high concentrations (>2 mM) of EGTA. These results suggest that ATP·induced histamine release is related to both intraoellular calcium release and extracellular calcium influx via voltage-dependent calcium channel and receptor-operated calcium channel. C48/80-induced histamine release is related to extracellular calcium influx, specially recetor-operated calcium channel rather tllan voltage-dependent calcium channel.

Endothelin-1에 의한 phospholipase C 활성화와 세포내 Ca2+ 이동에 미치는 protein kinase 들의 효과

조중형(Jung Hyung Cho),김현준(Hyun Jun Kim),이윤혜(Yun Hye Lee),박진형(Jin Hyoung Park),장용운(Youn Un Jang),이승준(Seung June Lee),이준한(June Han Lee),윤정이(Jeong Yi Yoon),김창종(Chang Jong Kim),심상수(Sang Soo Sim) 대한약학회 2000 약학회지 Vol.44 No.2

To investigate the effects of protein kinases on endothelin-l-induced phospholipase C activation and Ca2+ mobilization in Rat-2 fibroblast, we measured the formation of inositol phosphates and intracellular Ca2+ concentration with [3H]inositol and Fura-2/AM, respectively. Endothelin-1 dose-dependently activated phospholipase C and increased intracellular Ca2+ concentration. Protein kinase C activator, PMA, significantly inhibited both phospholipase C activity and Ca2+ mobilization induced by endothelin-1. Tyrosine kinase inhibitor, genistein, inhibited both. On the other hand, cyclic nucleotide (cAMP and cGMP) did not have any influence on the signaling pathway of phospholipase C-Ca2+ mobilization induced by endothelin-1. These results suggest that protein kinase C and tyrosine kinase counteract on the signaling pathway of phospholipase C-Ca2+ mobilization induced by endothelin-1 in Rat-2 fibroblast.

급성 신부전에서의 필수 아미노산으로 구성된 TPN의 효과

서영희,윤정이,장형원,이명덕 한국병원약사회 2002 병원약사회지 Vol.19 No.3

Morbidity and mortality in acute renal failure(ARF) remain high despite the early and aggressive therapy of dialysis and other medical advances. ARF is characterized by consistently protein catabolic body wasting which may have adverse effects on the outcome of their illness in the presence of excessive nitrogen retention. The patients with ARF frequently are uremic symptoms and suffer from malnutrition by therapy including dialysis, so require nutritional support. But among the nutrients, unselected protein cannot be administrated to patients with ARF because of aggravation of uremic symptoms. Recently, several studies have suggested that patients with ARF may benefit from treatment with infusions of small quantities of essential amino acids(EAA). The metabolic effect of intravenous EAA based Total Parenteral Nutirition(TPN) (NETNA ; Total Nutrient Admixture Formula) in the treatment of ARF retrospectively was evaluated. From June 1998 through July 2002, of all patients(n=38) at Kangnam St. Mary's hospital with ARF received NETNA, 8 patients met most of the following criteria in this study ; without dialysis and the administration time over 5 days. The time interval between the renal injury leading to renal failure and the beginning of therapy with NETNA was 17.2days. The time of NETNA administration was 16.4days. The mean total calories before treatment was 676.5㎉/d and after was 1682.5㎉/d(1378 ㎉ - 80% as NETNA). The mean blood urea nitrogen level decreased before treatment from 67.9±43.2㎎/㎗ to 35.9±38.2㎎/㎗ and the mean serum creatinine level dropped markedly from 5.59±4.3㎎/㎗ to 2.3±1.7㎎/㎗. The mean plasma potassium and magnesium concentration were unchanged by the treatment and remained within the normal range. The mean phosphate level statistically significantly dropped from 5.13±0.25㎎/㎗ to 2.93±1.40㎎/㎗(p<0.05). The mean cholesterol and triglyceride concentration remained within the normal range. Of 8 patients, 5 was carried out hemodialysis before NETNA administration, they all were stopped after administraion of NETNA. Intravenous administration of EAA based on TPN resulted in a stabilization of blood urea nitrogen levels and improved nutritional state with perhaps more rapid recovery of renal function. Adequate nutrition support could be performed in ARF without the need for additional therapy for the metabolic consequence of uremia(ex. dialysis).

정맥영양액과 5-FU의 Y-site 투여시 안정성

안혜진,서영희,윤정이,홍경자,라현오,윤석중 한국병원약사회 2001 병원약사회지 Vol.18 No.3

The compatibility of 5-fluorouracil (5-FU) with Parenteral Nutrient (PN) solution during simulated Y-site administration was studied. Five mililiter of representative PN solution was combined in duplicate in a 1:1 ratio with 5 ㎖ sample of solution of 5-FU in 5% dextrose injection (25, 50, 75, 100 ㎎/㎗). Chemical compatibility was examined the HPLC. The test was performed at intervals up to fourteen hours ; storage was at room temperature. Physical compatibility was performed for nonsoluble particle sizing and counting. All evaluations were performed at intervals up to six hours. The test group result of HPLC test were reduced 7.9, 7.6, 4.5, 7.9% in the 25, 50, 75, 100 ㎎/㎗. The blind group test were reduced 10.6, 8.7, 6.5, 5.8% in the 25, 50, 75, 100 ㎎/㎗. The result of nonsoluble particle sizing and counting were suitable for KSP criteria. During simulated Y-site administration, PN solution was compatible with 5-FU solution.

혈액투석으로 전환하여 성공적으로 치료한 복막투석치료중인 당뇨병성 만성신부전환자에서 발생한 고삼투압성 혼수

구자룡,이형철,김형직,채동완,박규용,노정우,권성희,이준상,전로원,고경식,박경창,윤정이,김근호 대한내과학회 1999 대한내과학회지 Vol.57 No.5

Continuous ambulatory peritoneal dialysis(CAPD) is now the most important and effective therapeutic modality as well as hemodialysis(HD) and renal transplantation in patients with chronic renal failure. It is frequently recommended to diabetic renal failure patients because of poor, athersclerotic vascularity of them. Hyperglycemia and obesity are not uncommon complications of CAPD therapy. But there were only few reports of very severe hyperglycemia or hyperosmolar coma in CRF patients on CAPD therapy, especially with 7% dextrose CAPD dialysate in foreign countries in the past. Moreover, no specific management for hyperosmolar coma is established in those situations yet. In Korea, only three cases of hyperosmolar coma in non diabetic renal failure patient on CAPD therapy have been reported in one case report. And only one case among them whose CAPD therapy was changed to HD survived. Authors also experienced a case of diabetic hyperosmolar coma treated successfully with conversion to HD in chronic renal failure patient on CAPD therapy. So we report this case with a review of literature. Taken together with this case and review of literature, changing CAPD therapy to HD is regarded to be an important part of treatment when hyperosmolar coma develops in chronic renal failure patients. (Korean. J. Med 57:946-949, 1999)