배란장애를 동반한 다낭성 난소인 여성에서 혈중 Inhibin 농도의 증가

노재숙,유중배,문형,황윤영,Roh, Jae-Sook,Yoo, Jung-Bae,Moon, Hyung,Hwang, Yoon-Yeong 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.1

Normal and abnormal follicular growth and steroidogenesis depend on gonadotropins as well as intraovarian peptides, which may mediate or potentiate gonadotropin action. Inhibin also affect follicular development and steroidogenesis and may play a role in dominant follicle selection and follicular atresia. Therefore, we studied the differences of serum inhibin, gonadotropin and androgen levels in the women with only the ultrasound findings and no disorder, and polycystic ovary (PCO) with ovulatory disturbance. We prospectively analysed forty-three women with PCO. The diagnosis of PCO was based on typical appearance of the ovaries on TVS. Twelve women with regular menstrual cycle and normal ovarian morphology were selected as control. Basal levels of inhibin, luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol $(E_2)$, testosterone (T), androstenedione (ADD), dehydroepiandrosterone-sulfate (DS), prolactin and TSH in serum were determined. There were significant differences in basal LH levels and LH/FSH ratio between the control and the women with PCO. The basal levels of inhibin and $E_2$ in the oligo-amenorrheic PCO (N=34) were significantly higher than those in the control. There was higher negative correlation between the inhibin and T levels in the oligo-amenorrheic PCO, but, not in the regular cycling PCO. Also, there was higher positive correlation between the LH and T levels in the oligo-amenorrheic PCO, but not in the regular cycling PCO. These data presume that the initial event of PCO is elevated pituitary LH secretion. Elevated levels of LH may down-regulate LH receptors on granulosa cells and also cause hypertrophy of the thecal layer. High level of androgen secreted by the hypertrophied thecal layer may stimulate inhibin secretion from granulosa cells and can be converted to estrogen by extraovarian tissues and could serve to augment pituitary sensitivity to GnRH with a resultant secretion of more LH than FSH. Inhibin may inhibit FSH action on granulosa cell in the PCO follicle, impairing follicular development and dominant follicle selection resulted in ovulatory disturbance.

정상 및 자궁경부무력증의 임신중 경부 길이 비교

라명재 ( Myung Jae Ra ),유중배 ( Jung Bae Yoo ),박문일 ( Moon Il Park ),노재숙 ( Jae Sook Roh ),나도수 ( Do Soo Nah ),문형 ( Hyung Moon ),황윤영 ( Yoon Yeoung Hwang ) 대한주산의학회 1996 Perinatology Vol.7 No.2

컴퓨터를 이용한 태아심박동의 객관적 분석: 만삭임신에서 태동에 따른 기본 태아심박동수의 변화

박문일 ( Moon Il Park ),유중배 ( Jung Bae Yoo ),고승권 ( Seung Kwon Koh ) 대한주산의학회 1996 Perinatology Vol.7 No.1

일측 태아사망이 동반된 쌍태임신의 연구

신중식 ( Joong Sik Shin ),이은경 ( Eun Kyung Lee ),유중배 ( Jung Bae Yoo ) 대한주산의학회 2001 Perinatology Vol.12 No.2

Meckel-Gruber 증후군 2예

신중식 ( Joong Sik Shin ),김태희 ( Tae Hee Kim ),김지영 ( Ji Young Kim ),유중배 ( Jung Bae Yoo ) 대한주산의학회 2001 Perinatology Vol.12 No.4

산전 진찰된 무두무심 쌍태아 2례

김태희 ( Tae Hee Kim ),권황 ( Hwang Kwon ),신중식 ( Joong Sik Shin ),유중배 ( Jung Bae Yoo ),정창조 ( Chang Jo Chung ) 대한주산의학회 2001 Perinatology Vol.12 No.4

사람의 난관 상피세포에서 인슐린유사 성장인자 - II 의 발현

노재숙(Jae Sook Roh),성노현(Ro Hyun Sung),신중식(Joong Sik Shin),유중배(Jung Bae Yoo),황윤영(Youn Yeung Hwang) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.9

연구목적 : 인슐린유사성장인자-II가 여성의 자궁이나 난소조직의 주기적인 변화에 관여하는 것은 잘 알려져 있으나, 난관 상피세포의 주기적인 변화와도 관련이 있는지는 명확치 않다. 본 연구는 난관 상피세포에서 IGF-II가 발현되는지를 알아보고, 발현되는 경우 생식주기에 따른 차이를 보이는지 비교하였다. 연구대상 및 방법 : 다양한 부인과 질환으로 개복수술을 받았던 월경주기가 규칙적인 16명의 가임기 여성과 4명의 폐경기 여성에서 난관조직을 얻어 IGF-II에 대한 면역조직염색을 시행하였다. 연구결과: IGF-II는 주로 난관 상피세포에서 강하게 발현되었고 기질세포와 근육층에서는 약하게 발현되었다. 상피세포 중에서 섬모가 있는 세포와 없는 세포간에 차이는 없었다. 난관 상피세포에서 IGF-II의 발현정도는 생식주기에 따른 차이를 보여, 증식기 중,후기와 분비기 초,중기가 증식기 초기 및 분비기 후기와 비교하여 강하게 발현되고 폐경기에서는 발현되지 않았다. 결 론 : 본 연구결과 가임기 여성의 난관 상피세포에 IGF-II가 발현된다는 것을 알 수 있었다. 이는 IGF-II가 직, 간접적으로 난관 기능에 관여한다는 것을 의미하며, 또한 주기에 따른 발현 정도의 차이는 IGF-II가 난소에서 분비되는 스테로이드 호르몬의 영향을 받는다는 것을 의미한다. Objective : The involvement of IGF-II in reproductive tissues, especially uterine and ovarian, there is very little information is available concerning their presence in human tubal epithelium. To elucidate this, the present study was undertaken to determine the pattern of expression and cyclic changes of IGF-II in human tubal epithelium during various reproductive stages including postmenopause. Methods : Tube samples from 16 normally cycling women, as well as from 4 postmenopausal women were obtained. The expression pattern of IGF-II was examined using immunohistochemistry. Results : The primary site of immunoreactivity for IGF-II in fallopian tube tissue was in the epithelial lining of the tubes, with substantially lower intensity in the stromal cells and smooth muscle layer. The immunostaining was associated with both cilicated and nonciliated tubal epithelial cells without substantial differences in their intensity. Immunostaining intensity of IGF-II in tubal epithelial cells was cycle-dependent and considerably higher in mid-late proliferative and early-mid secretory compared to early proliferative and late secretory phases of the menstrual cycle, with no immunostaining in the postmenopausal period. Conclusion : This study demonstrates the presence of IGF-II in the human fallopian tube during various stages of the menstrual cycle. These data suggest an autocrine or paracrine role for the IGF-II in fallopian tube function, and their cyclic dependency further implies ovarian steroidal regulation.

위 유문동 평활근 세포의 전기적 활동에 미치는 Vanadate의 효과

유중배,방효원,엄대용,이상돈 중앙대학교 의과대학 의과학연구소 1990 中央醫大誌 Vol.15 No.1

Effects of vanadate on the resting membrane potential and slow waves of the circular smooth muscle were observed using glass capillary microelectrodes. The obtained results were as follows: 1. Vanadate did not show any effects on; the slow waves of spontaneously beating preparations. 2. Variation of the resting membrane potential due to the change of outside K^+ was 20-28m V/decade which suggested the significant contribution of Na^+ to maintain the resting membrane potential. 3. Vanadate did not show any further effects on the depolarized state about l0mV by outside 20mM K^+. 4. Vanadate induced the depolarization in the low outside K^+ condition and the effect was exaggerated when Cal^(2+) was depleted. 5. Vanadate induced the depolarization in the low Na^+ and K^+ condition. It was conjectured that the effects of vanadate were enhancing the voltage-dependent calcium.

원발성 난관선암 1예

유주현,신성수,김경태,이재억,유중배,이기헌,황윤영 대한부인종양 콜포스코피학회 1993 Journal of Gynecologic Oncology Vol.4 No.2

Primary adenocarcinoma of the fall~opian tube is an extremely rare malignancy of the female genital tract. We had experienced a case of primary adenocarcinoma of the left fallopian tube which diagnosed ineidentally on the basis of the biopsy findings, and reported the case with a brief review of the concerned literatures.

다낭성 난소를 가진 여성에서 혈중 인슐린유사 성장인자와 결합단백질에 관한 연구

노재숙,유중배,김학순,조수현,황윤영 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.5

연구목적 : IGF 계열이 고안드로겐혈증 및 난포발달 장애에 관여하는지 여부는 명확치 않다. 본 연구는 IGF계열이 PCO에서 동반된 배란장애와 관련이 있는지 알아보고자 하였다. 연구대상 및 방법 : 첫째, 월경주기가 규칙적이고 정상난소를 가진 대조군(n=12)과 PCO(n=42)에서 혈중 FSH, LH, E2, ADD, T, 유리형태의 IGF-I과 IGF-II, 전체 IGFBP-1과 IGFBP-3의 기저농도를 측정하여 각각의 호르몬 값을 비교하였고, 둘째, 배란유도에 실패했던 PCO에서 난소전기소작술을 시행하여 시술 전 후, 각 호르몬의 변화를 관찰하였다. 셋째, 정상 난소와 PCO 난소에서 IGF-I, II의 발현정도에 차이가 있는지를 비교하였다. 연구결과 : PCO는 LH, ADD, IGF-II와 IGFBP-3의 혈중 기저농도가 증가되어 있으며, IGF-I은 감소 경향을 보였다. 또한 PCO에서는 LH와 free IGF-II 농도간에 연관관계가 있었으나(p=0.011, r2=0.3899), 대조군에서는 이러한 연관관계가 없었다. 시술을 받은 9명의 PCO는 모두 4주 이내에 자연배란이 되었고, 시술 전과 비교하여 시술 3주와 4주 후 혈중 LH 및 안드로겐(T, ADD)이 현저히 감소(p)되었고, 시술 3주 후 IGF-I과 IGFBP-3는 현저히 증가(p=0.0039, 0.0077)되었다. IGF-II와 IGFBP-1은 시술 전, 후 차이가 없었다. 난소에서 IGF-I과 IGF-II의 발현부위 및 발현정도는 정상 및 PCO 난소간에 차이가 없었다. 결론 : PCO에서 증가된 IGFBP-3는 IGF의 활성을 저하시킬 수 있을 것이다. 시술을 받은 PCO에서 혈중 IGF-I의 농도변화와 자연 배란주기의 회복은 IGF 계열이 PCO에서 난포발달 장애에 관여한다는 것을 의미하는 소견이다. Objective : The involvement of IGF system in hyperandrogenism and abnormal follicular development is controversial. This study is to assess whether IGF system contribute to it in the women with polycystic ovary(PCO). Methods : Baseline serum levels of luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E2), testosterone (T), androstenedione (ADD), prolactin, thyroid stimulating hormone (TSH), free insulin-like growth factor(IGF)-I, free IGF-II, insulin-like growth factor binding protein(IGFBP)-1, and IGFBP-3 were measured in twelve healthy regularly cycling volunteers and forty-two women with PCO then, the changes of baseline serum levels were evaluated after laparoscopic ovarian electrocauterization in nine PCO patients. In addition, the expression pattern of IGF-I and IGF-II was examined in the ovary of control and PCO group. Results : Baseline levels of LH, ADD, free IGF-II, and IGFBP-3 were significantly higher in PCO group. However, there were no significant differences in the levels of free IGF-I and IGFBP-1, although free IGF-I showed decreasing tendency in PCO group. And there was a significant positive correlation between the LH and free IGF-II level in the PCO(P=0.011, r2=0.3899), but not in the control. After ovarian electrocauterization, LH, T, and ADD levels decreased, and free IGF-I and IGFBP-3 level increased. While free IGF-II and IGFBP-1 level showed no significant changes. In the ovary, expression of both IGFs showed similar pattern in normal and PCO ovaries. Conclusion : The elevated IGFBP-3 level may alter the bioavailability of IGF(s) in the PCO. The change in IGF-I level and resumption of ovulation after electrocauterization, suggest a possible role of IGF system in the impairment of follicular development in the PCO.