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RISS 인기검색어
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- 저자 : 용동은(Dong Eun Young) (검색결과 4 건)
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CAPD 환자에서 도관 균락화와 합병된 Penicillium marneffei 복막염 1 예
강신욱(Shin Wook Kang),한대석(Dae Suk Han),이호영(Ho Yung Lee),한상훈(Sang Hoon Han),최훈영(Hoon Young Choi),이상철(Sang Cheol Lee),구영석(Young Suck Goo),장경희(Kyung Hee Chang),김준명(June Myung Kim),용동은(Dong Eun Young),이경원(K 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.4
P. marneffei is a fungus that causes life-threatening disseminated infection in a geographically distinct areas of the world. Following the first case of human infection in 1959, the incidence of this infection has risen markedly during the past 5 years. However, even in the midst of such rapid increase, the infection has always occurred only in a limited geographic distributions or in persons who have visited this limited geographic areas. These areas include Myanmar, Hong Kong, Indonesia, Laos, Malaysia, Singapore, Taiwan, Thailand, Vietnam, and the Guangxi province of southern China. P. marneffei infection occurs mostly in immunocompromised patients, particularly AIDS patients. P. marneffei infection commonly presents with skin and subcutanous tissue infection, fungemia, diarrhea, bone marrow infection, and generalized lymphadenopathy with hepatosplenomegaly. We report the case of continuous ambulatory peritoneal dialysis-associated peritonitis caused by P. marneffei. The case occurred in Korea, a non-endemic area of P. marneffei, in a non-AIDS patient who has not been exposed to any of the endemic areas. This warrants further consideration in determining the yet unknown transmission route of this fungal organism. P. marneffei was diagnosed without delay by 18sRNA PCR and sequencing, and was later confirmed by culture. PCR and sequencing may contribute to the early diagnosis of the P. marneffei infection, which is important given this infection`s ability to progress to a systemic infection with high mortality rate when diagnosis and management are delayed.
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화학발광자동면역분석기 Vitros ECi®를 이용한 TSH, Free Triiodothyronine, Free Thyroxine 및 Estradiol 검사 평가
용동은,선영규,이건수,김정호,권오헌 대한임상병리학회 2002 대한임상병리학회지 Vol.22 No.2
배경: 임상 검사실에서는 자동화 및 간편성의 요구가 커지는 한편 정확도, 민감도 및 직선성이 우수한 검사 방법이 요구된다. 저자들은 방사선면 역측정법(방사면역검사법)을 대체할 만한 화학발광 면역검사법을 적용한 Vitros ECi®Immunodiagnostic System에 의한 TSH, FT4, FT3 및 estradiol 검사를 평가하였다.<br>방법: TSH, free triiodothyronine (FT3), free thyroxine (FT4) 및 estradiol (E2)을 평가 대상으로 하였다. NCCLS기준에 의한 정밀도를 측정하였고, Elecsys 2010 및 방사면역검사법을 기준으로 분석하였다. TSH와 E2 에 대하여는 기능적 민감도와 직선성을 측정하였다. FT4 및 FT3에 대해서는 배수 희석법에 의한 유리 호르몬 검정을 실시하였다.<br> 결과: 정밀도는 네 종목 모두 우수하였고, TSH 및 E2의 기능적 민감도는 각각 0.007㎖μ/ℓ 및 87 p㏖/ℓ이었다.TSH는 최소 78 ㎖μ/㎖까지, E2는 최소 7,700 p㏖/ℓ까지 직선성이 유지되었다. 유리 호르몬 검정에서도 FT4와 FT3 각각 > 8.5p㏖/ℓ > 4 p㏖/ℓ로 감소되지 않았다. 참고방법과 상관성비교에서 TSH, FT4 및 FT3는 기울기 0.83-1.41의 비례오차를 보였고, E2의 경우 기울기 1.48-1.79의 비례오차가 있었다.<br> 결론: Vitros ECi®에 의한 TSH, FT3, FT4 및 E2 검사는 우수한 성능을 보여서, 기존의 방사면역검사법의 단점을 극복할수 있는 방법으로 평가되었다. Background: The analytical performance of the Vitros ECi® Immunodiagnostic System on the thyroid stimulating hormone (TSH), free triiodothyronine (FT3), free thyroxine (FT4) and estradiol (E2) assays, which are based on electrochemical luminescence to replace the radioimmunoassay was evaluated.<br> Methods: The precision as measured by the NCCLS protocol and a comparison of the method were done for each TSH, FT3, FT4, and E2 assay. The functional sensitivity and linearity tests were performed for both TSH and E2. The free hormone validity test was performed for both the FT3 and FT4.<br> Results: All four analytes showed an acceptable precision. The functional sensitivities of TSH and E2 were 0.007 ㎖μ/ℓ, and 87 p㏖/ℓ, respectively. TSH and E2 showed excellent linearity up to 78 ㎖μ/㎖, and up to 7,700 p㏖/ℓ, respectively. The free hormone validity test showed acceptable results demonstrating accurate free hormone determination. The E2 showed a significant proportional bias requiring an adjustment of the reference range, However, the other analytes showed good agreement with a slight proportional bias.<br> Conclusions: The TSH, FT3, FT4, and E2 assay by Vitros ECi® exhibited excellent performance overcoming the drawbacks of a conventional radioimmunoassay.
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