제55차 춘계학술대회 : 포스터 ; Quatroxir-AC 여드름 치료 효과 비교연구

오수아,서구일 대한피부과학회 2003 초록집 Vol.41 No.10

그래핀 나노 시트 위에 2차원 나노구조를 갖는 VO2의 성장

오수아(Su-Ar Oh),김기출(Ki-Chul Kim) 한국산학기술학회 2016 한국산학기술학회논문지 Vol.17 No.9

이산화바나듐은 섭씨 68도에서 금속-절연체 상전이 특성을 나타내는 써모크로믹(thermochromic) 소재로서, 상전이 현상이 일어날 때 광학적, 전기적 성질이 급격히 변화하며, 이러한 상전이 현상은 가역적인 특성을 가지고 있다. 이산화바나듐의 금속-절연체 상전이 현상을 응용하기 위하여 상전이 온도를 상온 부근으로 낮추고자하는 많은 시도들이 있었으며, 직경 100 nm의 1차원 나노구조를 갖는 이산화바나듐 나노와이어의 경우 29 oC 근처에서 상전이 현상이 일어남이 보고되었다. 본 연구에서는 기상 수송 방법(vapor transport method)을 사용하여 1차원 또는 2차원 나노구조를 갖는 이산화바나듐을 성장시켰다. 특히 동일한 성장 조건에서도 기판에 따라 다른 형태로 이산화바나듐이 성장하는 것을 확인하였다. 즉 Si 기판(Si\SiO2(300 nm) 위에서는 1차원 나노와이어 형태의 이산화바나듐이 성장하였고, 그래핀 나노시트 위에서 합성된 이산화바나듐은 2차원 또는 3차원 나노구조를 가지고 성장하였다. 바나듐 옥사이드 나노구조체의 성장에 사용된 Si 웨이퍼 위에 박리-전사된 그래핀 나노시트 기판과 thermal CVD 시스템으로 성장된 1D 또는 2D & 3D 나노 구조를 갖는 VO2의 결정학적 특성을 Raman 분광학으로 분석하였다. Raman 분석결과 성장된 바나듐 옥사이드는 VO2 상을 갖는 것을 확인하였다. Vanadium dioxide, VO2, is a thermochromic material that exhibits a reversible metal-insulator phase transition at 68 oC, which accompanies rapid changes in the optical and electronic properties. To decrease the transition temperature around room temperature, a number of studies have been performed. The phase transition temperature of 1D nanowire VO2 with a 100 nm diameter was reported to be approximately 29 oC. In this study, 1D or 2D nanostructured VO2 was grown using the vapor transport method. Vanadium dioxide has a different morphology with the same growth conditions for different substrates. The 1D nanowires VO2 were grown on a Si substrate (Si\SiO2(300 nm), whereas the 2D & 3D nanostructured VO2 were grown on an exfoliated grapheme nanosheet. The crystallographic properties of the 1D or 2D & 3D nanostructured VO2, which were grown by thermal CVD, and exfoliated-transferred graphene nanosheets on a Si wafer which was used as substrate for the vanadium oxide nanostructures, were analyzed by Raman spectroscopy. The as-grown vanadium oxide nanostructures have a VO2 phase, which are confirmed by Raman spectroscopy.

인간 포미바이러스 인테그라제의 핵위치 신호

오수아(Soo A Oh),강승이(Seung Yi Kang),한성태(Sung Tae Han),안덕근(Dog Gn An),신차균(Cha-Gyun Shin) 대한약학회 2006 약학회지 Vol.50 No.2

Human foamy virus (HFV) integrase mediates integration of viral c-DNA into cellular DNA. In this process, HFV preintegration complex (PIC) in which integrase is a key component moves to nuclei of the infected cells and leads to integration of viral DNA to the cellular genome, which is essential in viral life cycle. In general nuclear localization signals (NLS) have been suggested to be involved in localizing retroviral PIC to muclei, but the mechanisms for nuclear localization of the HFV PIC remains unclear. To functionally identufy the NLS of HFV integrase, various subdomains of the protein were expressed as GFP fusions and fusions and their subcellular locations were analyzed with comfocal laser scanning microscopy. Wild type HFV integrase was karyophilic by targeting the fusion protein to nuclei of the COS-1 and 294T cells. Our results showed that strong NLS of HFV integrase was mapped to the C-terminal regions. In addition the karyophimic properties of N-ter- minal and central regions are not individually strong enough to direct localization of the fusion proteins to nuclei, but their cooperative activity for nuclear inport was comfirmed.

골수유핵세포의 경내피세포 및 경기질세포 이행 과정에서 류코트리엔 B_(4) 역할과 활성산소종 억제의 영향

문영철,오수아,유은선,최문영,안지영,성주명 이화여자대학교 의과학연구소 2008 EMJ (Ewha medical journal) Vol.31 No.2

Objectives:Leukotriene B_(4)(LTB4) is lipid mediator derived from membrane phospholipids during the process of inflammation, having many roles(ie;inducer of chemotaxis, the production of nitric oxide, transepithelial migration of neutrophil). The major activities of LTB4 include the recruitment and activation of leukocytes, suggesting that it may involve the process for transendothelial migration of nuclear cells in bone marrow environment. Reactive Oxygen Species (ROS) have a cell signaling roles that are involved in signal transduction cascades of numerous growth factor-, cytokine-, and hormone-mediated pathways, and regulate many biological systems. In this present study, we focused on the role of LTB4 and ROS on transmigration of bone marrow nuclear cells across endothelial or stromal cell monolayer. Methods:MS-5, murine stromal cell line cells, or bEnd.3, murine microvascular cell line cells, were grown to confluence on microporous transwell membrane. Murine marrow cells were placed on top of the prepared transwell membrane. The transwells were then seated in wells containing media and LTB4 with or without pretreatment of N-acetylcysteine(NAC), an oxygen free radical scavenger, or diphenylene iodonium(DPI), an inhibitor of NADPH oxidase-like flavoproteins. Cells that migrated through the stromal or endothelial layer into the wells were assayed for transendothelial migration. Results:The numbers of migrated bone marrow nuclear cells through the bEnd.3 were increased by treatment of LTB4(control, 12.5±0.2%;50nM, 22.7±0.9%;100nM, 44.3±1.4%;200nM, 36.3±0.9%;p<0.05). The numbers of migrated bone marrow nuclear cells through the MS-5 were also increased by treatment of LTB4(control, 11.0±0.9%;50nM, 25.7±0.9%;100nM, 35.8±1.8%;200nM, 32.1±0.9%;p<0.05). However, increasing effect of LTB4 to the transmi-gration of bone marrow nuclear cells through the MS-5 or bEnd.3 were inhibited by pretreatment of NAC or DPI. Conclusion:Through our data, it is suggested that LTB4 could induce the transmigration of bone marrow nuclear cells and ROS might be involved on the transendothelial migration of bone marrow nuclear cells by LTB4. It would be very interesting to test the effects of LTB4 and ROS on stem cell mobilization and homing in the future. 류코트리엔 B_(4)(LTB4)는 세포막 인지질로부터 유래된 염증과정에 관여하는 지방매개체이다1-3). LTB4의 주된 역할은 화학주성의 유도, 활성산소의 생산, 백혈구, 특히, 호중구 및 호산구의 이동에 관여하고 있으며, 내피세포와 백혈구의 부착에 중요한 역할을 하는데, 이러한 LTB4의 역할은 케모카인에 의한 백혈구의 이동과 화학주성과 많은 부분에서 유사한 특성을 가진다4-7). 염증반응과 백혈구 이동과 연관된 LTB4의 역할들은 골수내 세포들의 이동 과정에서 다른 시토카인이나 성장인자 혹은 케모카인의 역할과 유사한 점이 많다. LTB4와 비슷한 작용을 가진 여러 케모카인들이 조혈모세포를 포함한 골수내 세포들의 이동과 연관이 있음이 최근 밝혀짐에 따라, LTB4도 골수내 세포의 이동에 관련이 있을 가능성이 있다8-14). 활성산소종(ROS)은 여러 종류의 성장인자, 시토카인, 혹은 호르몬 등으로 유도된 조혈세포내 신호전달체계에 중요한 역할을 하고, 조혈세포내 여러 생명현상의 조절과 관련이 있다15-18). 특히, 염증세포에서 분비된 시토카인 혹은 케모카인들이 세포내 ROS를 증가시키고, 이때 증가된 ROS는 혈관내피세포 단층의 투과성을 증가시켜 백혈구의 경내피세포 이행을 용이하게 한다19-22). 조혈모세포 이식 후 조혈모세포가 말초혈액에서 골수 내로 자리잡는 귀소나, 골수내 조혈모세포를 혈액으로 나오게 하는 가동화의 과정은 매우 복잡하여, 여러 다양한 기전들이 관련이 있지만, 혈관내피세포 및 기질세포와 골수 조혈세포들과의 유기적인 반응과 골수 조혈세포의 경내피세포 이행이 중요한 역할을 한다23)24). 본 연구에서는 골수유핵세포의 경내피세포 및 경기질세포 이행에 LTB4와 ROS가 어떻게 영향을 미치는지 알아보고자 하였다.

인간 포미바이러스 인테그라제의 생화학적 특성

강승이,오수아,이학성,한성태,서진욱,신차균 대한약학회 2004 약학회지 Vol.48 No.1

A bacterial expression vector for the human foamy virus (HFV) integrase was constructed and expressed in Escherichia coli. By two-step purification using a nickel-chelated column and a SP-sepharose chromatography; the HFV into-grase protein of 43 kDa was purified to near homogeneity, and used to investigate biochemical characteristics of the enzymatic activities, such as endonucleolytic and disintegration activities. Oligonucleotide substrates were specifically and efficiently cleaved by the purifed HFV integrase in the presence of Mn $^{+2}$, but not in the presence of Mg $^{+2}$, indicating that the HFV integrase is not able to use Mg $^{+2}$ as a cofactor Endonucleolytic reaction was almost completed in 60 min at 37 $^{\circ}C$. In addition, the maximum enzymatic activities were observed at 5 mM Mn $^{+2}$ in the buffer of which pH was from 7.0 to 9.0. The endonucleolytic activities were dose-dependently blocked in the addition of baicalein or chicolic acid which is a well-known inhibitor of human immunodeficiency virus integrase.

황사 마스크 이용 및 관리 실태

김재영,오수아,이송은,박수진,박경은,노수용,이지훈,김호길,송재철 대한직업환경의학회 2014 대한직업환경의학회 학술대회 논문집 Vol.2014 No.11

제55차 춘계학술대회 : 포스터 ; 제모레이저를 이용한 좁은 이마 확장술 60예

조흔정,변재철,오수아,서구일 대한피부과학회 2003 초록집 Vol.41 No.10

인간 포미바이러스 인테그라제 돌연변이의 특성

강승이(Seung Yi Kang),오수아(Soo A Oh),이학성(Hak Sung Lee),한성태(Sung Tai Han),신차균(Cha-Gyun Shin) 대한약학회 2005 약학회지 Vol.49 No.3

Human foamy virus (HFV) integrase mediates integration of viral c-DNA into cellular DNA. In this process, HFV integrase recognizes its own viral DNA specifically and catalyzes insertion of viral c-DNA. In order to study catalytic domains and residues, three deletion mutants and two point mutants of HFV integrase were constructed and analyzed with respect to enzymatic activities. The C-terminal deletion mutant showed decreased enzymatic activities while the N-terminal deletion mutant lost the activities completely, indicating that the N-terminal domain is more important than the C-terminal domain enzymatic reaction. The point mutants, in which an aspartic acid at the 164th position or a glutamic acid at the 200th position of The HFV integrase protein was changed to alanine, lost the enzymatic activities completely. However, they results suggest that the aspartic acid and glutamic acid at the respective 164th and 200th positions are catalytic residues for enzymatic reaction.

골수채취시 급성과량성 혈액희석이 혈액손실 및 이식단핵세포수에 미치는 영향

최진환,문세호,홍성진,전진영,성춘호,이지영,오수아,최정환 대한마취과학회 2000 Korean Journal of Anesthesiology Vol.38 No.1

Background : Hypervolemic hemodilution (HHD) seems to be a good alternative to acute normovolemic hemodilution to reduce transfusion requirements because it requires Bess time and effort. In a prospective study in healthy donors, we studied the effects of HHD on blood loss and menonuclear cell counts of aspirated bone marrow which were transplanted. Methods : After induction of anesthesia and positioning to prone, donors of the HHD group (n = 25) were administered 7.5 ml/kg of pentastarch and the same amount of Hartmanns solution prior to aspiration, but those of the control group (n = 25) were administered only 2 ml/kg/h of Hartmann's solution, Bone marrow was aspinted at the iliac spine and blood loss was replaced with pentastarch and Hartmann's solution for both groups. Hemoglobins and mononuclear were counted after induction, after hemodilution and the end of aspiration. Hemoglobins and mononuclear cell counts of aspirated bone marrow were also counted. Results: HHD decreased hemoglobin from 13.2 ± 1. g/dl to 10.7 ± 1.0 g/dl significantly (P $lt; 0.01). Even though hemoglobins of both groups at the end of aspiration were not different (9.3 ± 2.1 g/dl at control vs 9.5±1.1 g/dl at HHD), caloulated b]ooh loss based on body weight through aspirated bone marrrow was less in the HHD group (18.0±1,8% at control vs 16.3±2.0% at HHD, P $lt; 0.05). There was no difference in counted mononuclear cell counts between the two groups (1.8 ±0.7×10(7)/m1 at control vs 1.8 ± 0.7 × 10(7)/ml at HHD). Conclusions : During bone marrow harvesting in healthy donors, HHD reduced blood loss through aspirated bone marrow, but does not affect transplanted bone marrow mononuclear cell counts. (Korean J Anesthesial 2000; 38: 25∼29)

데이터주도 임상 서비스를 위한 사용자 활동기반 의료진단지원시스템

조현상(Hyun Sang Cho),김성은(Sung Eun Kim),이고은(Ko-Eun Lee),오수아(Su A Oh),김정의(Jeong Ui Kim) 한국정보과학회 2021 정보과학회 컴퓨팅의 실제 논문지 Vol.27 No.5

정보통신기술과 IoT의 발달은 병원에서만 이루어지던 진료와 치료의 영역을 가정을 포함한 영역으로 확장하여 얻어지는 방대한 데이터를 이용하여 질병의 발생을 예측하고 방지하는 4P(Personalized, Participatory, Predictive, Preventive) 정밀 의료를 가능하게 한다. 생활패턴에서 얻을 수 있는 사용자의 활동정보는 내적, 외적인 요인에 의해 발생한 질병에 의해 영향을 받을 수 있으며, 특정한 질병은 특정한 사용자 활동에 영향을 미치게 된다. 본 논문은 의료진단을 지원하고 interactive 서비스와 결합하여 질환의 치료를 포함한 cybernetics system으로 활용될 수 있는 사용자 활동 데이터 기반의 의료 서비스 시스템의 개념모델을 제안한다. The development of information and communication technology and IoT enables 4P (personalization, participation, prediction, and prevention) precision medicine that predicts and prevents diseases by using vast amounts of data obtained by expanding the diagnosis and treatment areas of hospitals into homes. It is hypothesized that the user"s activity data, which can be obtained from the living patterns, may be affected by disease due to certain internal and external factors. Moreover, a specific disease affects a specific user activity. In this paper, we propose a conceptual model of a medical service system based on the user"s activity data that supports medical diagnosis and can be used as a cybernetics system for digital therapeutics in combination with interactive services.