웰빙 라이프스타일이 자연주의 화장품의 지각된 가치와 구매의도에 미치는 영향

오만호,박현정,이상환,박수용 한국피부과학연구원 2010 대한피부미용학회지 Vol.8 No.3

With the focus on health and the nature based on theoretical bases, the effect of well-being lifestyle that was introduced by the wellness trends on the perceived value of naturalistic cosmetics was examined and the effect of perceived value of naturalistic cosmetics on purchase intention was studied as well. The well-being lifestyle was divided into positive sports/hobby activities, healthy diet, recycling activities, interest in welfare of senior citizens, interest in aging, and interest in fashion/appearance. The research model was suggested for the relationship between the well-being lifestyle and the perceived value and purchase intention of naturalistic cosmetics. The data was collected through the questionnaire survey which was conducted on persons in their twenties, thirties and forties who had high use and frequent purchase of cosmetics. A total of 315 persons responded to the survey. The positive research findings are summarized as below. First, as a result of examining the relationship between well-being lifestyle and the perceived value of naturalistic cosmetics, the interests in healthy diet, recycling activities, interest in aging, and interest in fashion/appearance had positive effects on the perceived value. That means those who pursued healthy diet, put into practice recycling activities and had high interest in aging and fashion/appearance placed high value on the perceived value of naturalistic cosmetics. Second, as a result of studying the relationship between the perceived value and the purchase intention of naturalistic cosmetics, it turned out that the perceived value had positive effects on the purchase intention of naturalistic cosmetics. That is, the higher value placed on the perceived value of naturalistic cosmetics resulted in stronger purchase intention. 현대인들의 주요 관심사인 건강과 자연에 핵심을 두고 웰빙트렌드에 의해 등장한 웰빙 라이프스타일이 자연주의 화장품의 지각된 가치에 미치는 영향을 연구하고 자연주의 화장품의 지각된 가치가 구매의도에 미치는 영향을 연구하였다. 웰빙 라이프스타일을 적극적 스포츠/취미활동, 건강 식생활, 재활용 실천, 노후복지에 대한 관심, 노화에 대한 관심, 패션․외모에 대한 관심으로 구분하고 웰빙 라이프스타일과 자연주의 화장품의 지각된 가치와 구매의도 간의 관계를 연구모형으로 제시하였다. 설문조사를 통해 데이터를 수집하였으며, 설문조사는 화장품의 사용 및 구매빈도가 높은 20대~40대를 대상으로 하였으며, 분석에 사용된 응답자수는 총 315명이다. 실증적 연구결과는 다음과 같이 요약할 수 있다. 첫째, 웰빙 라이프스타일과 자연주의 화장품의 지각된 가치와의 영향관계를 검정한 결과, 건강 식생활, 재활용 실천, 노화에 대한 관심, 패션·외모에 대한 관심은 자연주의 화장품의 지각된 가치에 정(+)의 영향을 주는 것으로 나타났다. 즉, 건강 식생활을 지향하고 재활용 활동을 직접 실천하고 노화에 대한 관심이 높고 패션·외모에 대한 관심이 높을수록 자연주의 화장품에 대한 지각된 가치를 높게 평가한다는 것을 알 수 있다. 둘째, 자연주의 화장품의 지각된 가치와 구매의도와의 영향관계를 검정한 결과, 자연주의 화장품의 지각된 가치는 자연주의 화장품의 구매의도에 정(+)의 영향을 미치는 것으로 나타났다. 즉, 자연주의 화장품에 대한 지각된 가치를 높게 평가할수록 자연주의 화장품을 구매하고자 하는 의도가 높다는 것을 알 수 있다.

커피숍 친환경 마케팅 활동이 고객 충성도에 미치는 영향

오만호(Wan Hao Wu),유소이(So Ye You),진대건(Dae Gun Jin) 한국지역사회생활과학회 2018 한국지역사회생활과학회 학술대회 자료집 Vol.2018 No.4

폐튜니아 원형질체 유래 재분화 개체의 유전적 변이

김상구,오만호 한국유전학회 1988 Genes & Genomics Vol.10 No.4

Protoplasts isolated from leaf mesophyll tissue of petunia (Petunia hybrids) were cultured on the modified MS medium. Three hundred and eighty protoclones were randomly selected from protoplast-derived cell colonies. Among them shoot regenerations occurred from 45 protoclones, and 130 regenerants were obtained. Root tip chromosome numbers were identified in 60 regenerants. Six diploid plants from 1 protoclone retained the parental chromosome number of 2n=14. Of the remaining plants, regenerants were identified as 3 aneuploids from 1 protoclone and 51 tetraploids from 43 protoclones. Morphological characteristics of regenerated plants have been observed in the greenhouse. Aneuploid plants were abnormal in morphology and fertility and tetraploids were larger in leaf, petal and sepal than those of normal diploids.

애기장대 SHL1 단백질의 TCH4 프로모터의 특정 염기 서열 결합에 관한 연구

이지혜,오만호,Lee, Ji Hyea,Oh, Man-Ho 한국식물생명공학회 2018 식물생명공학회지 Vol.45 No.1

The Arabidopsis SHL1 (${\underline{Sh}}ort$ ${\underline{L}}ife$ 1) gene encodes a small nuclear protein that is critical for the proper expression of the developmental programs that are responsible for controlling plant stature, senescence, flowering and seed formation. The SHL1 contains a single PHD finger domain that works in conjunction with a bromo-adjacent homology (BAH) motif that is thought to function significantly in protein-protein interactions. The TCH4 gene of the Arabidopsis encodes a xylogluclan endotransglucosylase/hydrolase that is transcriptionally regulated by a variety of hormonal and environmental stimuli. We report here in this study that the SHL1 exhibits sequence specific DNA binding properties, recognizing a 14 bp region of the TCH4 promoter in vitro, spanning nucleotides -262 to -275 (GGAAAAAACTCCCA). Chiefly, the nuclear extracts of Arabidopsis contain a protein with similar binding properties as recombinant SHL1, which is absent in identified transgenic plants that are noted as expressing antisense SHL1 RNA. Interestingly, the SHL1 gene expression with a BL treatment in characteristically wild types of seedlings showed that the transcript level of SHL1 is significantly down regulated by the BL treatment. The SHL1 may play a subtle role in regulating the kinetics of induction of the TCH4 in response to several stimuli in vivo.

애기장대 SHL1 단백질의 TCH4 프로모터의 특정 염기 서열 결합에 관한 연구

이지혜,오만호 한국식물생명공학회 2018 JOURNAL OF PLANT BIOTECHNOLOGY Vol.45 No.1

The Arabidopsis SHL1 (Short Life 1) gene encodes a small nuclear protein that is critical for the proper expression of the developmental programs that are responsible for controlling plant stature, senescence, flowering and seed formation. The SHL1 contains a single PHD finger domain that works in conjunction with a bromo-adjacent homology (BAH) motif that is thought to function significantly in protein-protein interactions. The TCH4 gene of the Arabidopsis encodes a xylogluclan endotransglucosylase/hydrolase that is transcriptionally regulated by a variety of hormonal and environmental stimuli. We report here in this study that the SHL1 exhibits sequence specific DNA binding properties, recognizing a 14 bp region of the TCH4 promoter in vitro, spanning nucleotides –262 to –275 (GGAAAAAACTCCCA). Chiefly, the nuclear extracts of Arabidopsis contain a protein with similar binding properties as recombinant SHL1, which is absent in identified transgenic plants that are noted as expressing antisense SHL1 RNA. Interestingly, the SHL1 gene expression with a BL treatment in characteristically wild types of seedlings showed that the transcript level of SHL1 is significantly down regulated by the BL treatment. The SHL1 may play a subtle role in regulating the kinetics of induction of the TCH4 in response to several stimuli in vivo.

Different vegetative growth stages of Kimchi cabbage (Brassica rapa L.) exhibit specific glucosinolate composition and content

전병욱,오만호,김은옥,김형석,채원병 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.3

This study aimed to simultaneously investigate the changes in growth characteristics and glucosinolate (GL) content during different growth stages in Kimchi cabbage. Two Kimchi cabbage cultivars ‘Chuweol’ and ‘HwiparamGold’ were grown in the field and plant characteristics such as leaf length, number and fresh weight, and GL contents were measured weekly from 2 to 9 weeks after transplanting (WAT). The only significant difference between the two cultivars for either plant growth or GL content was observed for GL contents during 3 and 7 WAT. Leaf length increased until 4 WAT and then remained unchanged, exhibiting logarithmic growth. The fresh weight and number of leaves increased linearly until 9 WAT. Five GLs (two aliphatic GLs: progoitrin and gluconapin, two indole GSLs: glucobrassicin and neoglucobrassicin, and one aromatic GL: gluconasturtiin) of the nine GLs investigated in this study (glucoiberin, progoitrin, glucoraphanin, sinigrin, gluconapin, glucobrassicin, gluconasturtiin, 4-methoxy glucobrassicin and neoglucobrassin) were detected in the two cultivars. The contents of these five GLs were similar in the two cultivars during 2 WAT, but gluconapin and gluconasturtiin increased more dramatically than the others. The increasing pattern of total GL more closely resembled the leaf growth pattern than the fresh weight. Our results suggest that the change in total GL content positively correlates to leaf length, and the increase in total GL content is attributed to the increase in the amount of gluconasturtiin and gluconapin during the autumn growing season.

Tyrosine phosphorylation as a signaling component for plant improvement

박연일,양효식,오만호 한국식물생명공학회 2015 식물생명공학회지 Vol.42 No.4

Plant genome analyses, including Arabidopsis thaliana showed a large gene family of plant receptor kinases with various extracellular ligand-binding domain. Now intensively studies to understand physiological and cellular functions for higher plant receptor kinases in diverse and complex biological processes including plant growth, development, ligands perception including steroid hormone and plant-microbe interactions. Brassinosteroids (BRs) as a one of well know steroid hormone are plant growth hormones that control biomass accumulation and also tolerance to many biotic and abiotic stress conditions and hence are of relevance to agriculture. BRI1 receptor kinase, which is localized in plasma membrane in the cell sense BRs and it bind to a receptor protein known as BRASSINOSTEROID INSENSITIVE 1 (BRI1). Recently, we reported that BRI1 and its co-receptor, BRI1-ASSOCIATED KINASE (BAK1) autophosphorylated on tyrosine residue (s) in vitro and in vivo and thus are dual-specificity kinases. Other plant receptor kinases are also phosphorylated on tyrosine residue (s). Post-translational modifications (PTMs) can be studied by altering the residue modified by directed mutagenesis to mimic the modified state or to prevent the modification. These approaches are useful to not only characterize the regulatory role of a given modification, but may also provide opportunities for plant improvement.

지치(Lithospermum erythrorhizon)의 현탁배양 세포에서 시험관내 단백질 합성의 변화

김상구,유희주,오만호,리상범 한국유전학회 1993 Genes & Genomics Vol.15 No.4

We have previously isolated shikonin-producing and shikonin-nonproducing cell lines from the single cell cultures of Lithospermum erythrorhizon. In this study, in vitro translation products of poly(A)^+ RNA isolated from these different cell lines were analyzed by two-dimensional polyacrylamide gel electrophoresis and visualized by autoradiography by labeling proteins with ^(35)S-methionine. Comparison of translation products on 2-D SDS-PAGE showed the qualitative and quantitative changes in cell growth medium and pigment production medium. Especially, the shikonin-producing cell lines cultured on pigment-producing medium synthesized several new proteins. The result indicates that those proteins may be candidates for specific proteins related with shikonin production.

Expression and phosphorylation analysis of soluble proteins and membrane-localised receptor-like kinases from Arabidopsis thaliana in Escherichia coli

오은석,변환에바,김상윤,오만호 한국식물생명공학회 2018 JOURNAL OF PLANT BIOTECHNOLOGY Vol.45 No.4

Molecular and functional characterization of proteins and their levels is of great interest in understanding the mechanism of diverse cellular processes. In this study, we report on the convenient Escherichia coli-based protein expression system that allows recombinant of soluble proteins expression and cytosolic domain of membrane-localised kinases, followed by the detection of autophosphorylation activity in protein kinases. This approach is applied to regulatory proteins of Arabidopsis thaliana, including 14-3-3, calmodulin, calcium-dependent protein kinase, TERMINAL FLOWER 1(TFL1), FLOWERING LOCUS T (FT), receptorlike cytoplasmic kinase and cytoplasmic domain of leucine-rich repeat-receptor like kinase proteins. Our Western blot analysis which uses phospho-specific antibodies showed that five putative LRR-RLKs and two putative RLCKs have autophosphorylation activity in vitro on threonine and/or tyrosine residue(s), suggesting their potential role in signal transduction pathways. Our findings were also discussed in the broader context of recombinant expression and biochemical analysis of soluble and membrane-localised receptor kinases in microbial systems.

Brassinosteroid 호르몬 처리에 의한 케일 새싹채소 내 글루코시놀레이트 변화

천진혁 ( Jun-hyuk Chun ),오만호 ( Man-ho Oh ),김선주 ( Sun Ju Kim ) 한국환경농학회 2019 한국환경농학회 학술대회집 Vol.2019 No.-

Brassinosteroids (BRs)는 식물체 내에 존재하는 스트레스 완화성 호르몬으로, 고온 및 저온 스트레스, 가뭄, 염 동 환경 스트레스로부터 식물을 보호하는 역할을 한다. 식물 내에서 개화, 세포주기, 질소 대사, 스트레스 저항성에 관하여 신호전달을 하며, 이 호르몬의 신호전달은 세포막에 존재하는 BR 수용체인 BR Intesitive 1 (BRI1)에 의해 진행된다. 글루코시놀레이트 (glucosinolate, GSL)는 질소와 황을 포함하는 배추과 채소 내 이차 대사산물로 myrosinase 효소에 의한 가수분해로 isothiocyantate, thiocyanate 및 nitrile로 분해되어 활성을 나타내며, 간암, 위암 등에 항암 효과가 있고, 식물에서는 항균, 살충작용과 같은 생체방어 반응에 관여한다. GSL의 생합성이 BRs 호르몬의 작용 기작에 의해 조절된다는 선행 연구결과를 바탕으로 본 연구에서는 케일 새싹채소에 BR 호르몬을 처리하여 GSL 함량의 변화를 관찰하였다. 케일 품종은 ‘티비에스’, ‘만추콜라드’ 두 품종을 사용하였고, 식물생장기 (온도 25°C; 습도 70%)에서 7일간 재배하였다. 각 케일 새싹채소 실험구에 BR 호르몬 한 종류인 Brassinolide를 1μM씩 무처리, 30분, 90분, 4시간, 12시간 처리한 후, 액체질소로 동결 한 다음 동결건조하여 분석에 사용하였다. 케일 새싹채소에서 총 10종류의 GSL 성분이 검출되었고, 그 중 9가지 성분 (Glucoiberin, Progoitrin, Sinigrin, Gluconapin, 4-Hydroxyglucobrassicin, Glucobrassicanapin, Glucobrassicin, 4-Methoxyglucobrassicin, Neoglubrassicin)을 동정하였다. GSL 총 함량은 ‘티비에스’ 품종이 ‘만추콜라드’ 보다 높았으며, 항암 기능성 성분인 sinigrin 역시 ‘티비에스’ 품종에서 높았다. ‘티비에스’ 품종의 경우 BR 호르몬 처리시간이 증가할수록 sinigrin 및 GSL 총 함량이 증가하였으나, ‘만추콜라드’의 경우 무처리에 비해 BR 처리구에서 함량이 감소하였고, 증감에 경향성이 없었다. 향후, GSL 생합성 경로에 관여하는 MYB 유전자의 발현량을 검정하여, GSL 함량과의 상관관계를 분석하고, BR 호르몬이 GSL 생합성 경로에 관여하는 메커니즘을 규명하는 연구를 진행할 예정이다.