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Tyrosine phosphorylation as a signaling component for plant improvement
박연일,양효식,오만호 한국식물생명공학회 2015 식물생명공학회지 Vol.42 No.4
Plant genome analyses, including Arabidopsis thaliana showed a large gene family of plant receptor kinases with various extracellular ligand-binding domain. Now intensively studies to understand physiological and cellular functions for higher plant receptor kinases in diverse and complex biological processes including plant growth, development, ligands perception including steroid hormone and plant-microbe interactions. Brassinosteroids (BRs) as a one of well know steroid hormone are plant growth hormones that control biomass accumulation and also tolerance to many biotic and abiotic stress conditions and hence are of relevance to agriculture. BRI1 receptor kinase, which is localized in plasma membrane in the cell sense BRs and it bind to a receptor protein known as BRASSINOSTEROID INSENSITIVE 1 (BRI1). Recently, we reported that BRI1 and its co-receptor, BRI1-ASSOCIATED KINASE (BAK1) autophosphorylated on tyrosine residue (s) in vitro and in vivo and thus are dual-specificity kinases. Other plant receptor kinases are also phosphorylated on tyrosine residue (s). Post-translational modifications (PTMs) can be studied by altering the residue modified by directed mutagenesis to mimic the modified state or to prevent the modification. These approaches are useful to not only characterize the regulatory role of a given modification, but may also provide opportunities for plant improvement.
청록색광에 색성적응된 Anacystis niduland 의 상전이 증가
박연일,양덕조,홍영남 ( Youn Il Park,Duck Jo Yang,Young Nam Hong ) 생화학분자생물학회 1994 BMB Reports Vol.27 No.2
Photoacclimation of Anacystis nidulans toward blue-green light was investigated in terms of sensitivity of state transition. Ratio of phycobilin/chlorophyll and maximal photosynthetic rates of A. nidulans grown in blue-green light increased 7.6 and 1.5 times as large as those of white light-grown cells, respectively, but there was no apparnnt change in the energy transfer efficiency from phycocyanin to chlorophyll. The magnitude of state transition of blue-green light grown cells under preferential excitation of photosystem I by red light ($gt;630 ㎚) was increased by 38% in comparision with that of white-light grown cells. Uncoupler, m-chlorophenylhydrazone, and protein kinase inhibitor, 5`-p-fluorosulfonylbenzoyladenosine, severely inhibited the state 2 transition, but ATP synthase inhibitor, N,N`-dicyclohexylcarbodiimide, did the state 1/2 transitions. These results suggest that state transition in A. nidulons should be regulated by redox state of plastoquinone, and photoacclimation to blue-green light is related to the increased senstitivity of state transition.
박연일,신동호,최명구,강천식,박철수,최상봉 한국유전학회 2016 Genes & Genomics Vol.38 No.4
Plants of wheat cultivar Iksan370, which was recently produced by crossing paternal line Keumkang with maternal line Xian83, accumulate anthocyanins in their coleoptiles and seeds. However, the spatio-temporal regulation of anthocyanin biosynthesis genes in wheat remains poorly understood. Therefore, in the present study, we characterized wheat dihydroflavonol 4-reductase (DFR), which catalyzes the conversion of dihydroflavonol to leucoanthocyanidins during anthocyanin biosynthesis, using a heterologous expression system. The deduced amino acid sequence from full-length cDNA of wheat DFR cloned from young seedlings of Iksan370 (TaDFR-I) includes a well-conserved substrate-binding domain compared with previously identified DFRs. Furthermore, as expected, phylogenetic tree analysis placed TaDFR-I in the monocotyledonous clade. Introduction of TaDFR-I into the wild-type Arabidopsis Col-0 and dfr mutant backgrounds resulted in significant accumulation of anthocyanins in the respective transgenic plants. Similarly, TaDFR transcripts highly accumulated in Iksan370 wheat seedlings under various anthocyanin-inducing conditions, including low temperature, high salt and sugar levels, and UV-B illumination. Thus, we functionally characterized TaDFR-I from Iksan370 lines using a heterologous expression system and revealed the presence of transcriptional regulatory factors similar to those of Arabidopsis that regulate TaDFR expression in response to various abiotic stresses.
박연일,Jung-Mi Lee,류지연,Hyong-Ha Kim,최상봉,Nicole Tandeau de Marsac 한국분자세포생물학회 2005 Molecules and cells Vol.19 No.2
In silico analysis of genome of the cyanobacterium Synechocystis sp. PCC 6803 identified two genes, slr0329 and sll0593, that might participate in glucose (Glc) phosphorylation (www.kazusa.or.jp/cyano). In order to determine the functions of these two genes, we generated deletion mutants, and analyzed their phenotypes and enzymatic activities. In the presence of 10 mM Glc, wild-type (WT) and slr0329 defective strain (M1) grew fast with increased respiratory activity and NADPH production, whereas the sll0593 deletion mutant (M2) failed to show any of the Glc responses. WT and M1 were not significantly different in their glucokinase activity, but M2 had 90% less activity. Therefore, we propose that Sll0593 plays a major role in the phosphorylation of glucose in Synechocystis cells.
고온 스트레스가 Anacystis nidulans 의 광합성에 미치는 영향
박연일,양덕조,홍영남 ( Youn Il Park,Duck Jo Yang,Young Nam Hong ) 한국하천호수학회 1993 생태와 환경 Vol.26 No.1
4Effects of high temperature stress on the photosynthesis of Anacystis nidulans were investigated by using the measurements of oxygen exchange rates and room temperature chlorphyll fluorescence. With increasing pretreated-temperature for 5 min, oxygen evolution rates of A. nidulans were increased up to 50˚C , and then rapidly decreased. Especially, the oxygen evolution rates were inhibited by 50˚C(I_50) at 53˚C. These inhibition of photosynthesis by high temperature were caused by the restrictions of PS II activites. Hydroxylamine could not restore the oxygen evloution rates of PS II in A. nidulans pretreated with high temperature at 53˚C for 5 min. Ca^2+ pretreatment before temperature treatment for 5 min at 53˚C prevented PS II function from high temperature stress. Constant fluorescence level(Fo)-temperature curves showed the increase of F_O level with increaing heating temperature. However, Ca^2+ prevented increase of F_O from heat treatment. From the analysis of fast induction kinetics of room tenpe-rature chlorophyll fluorescence, F_O level was increased by 79%, but variable fluroescence(Fv) was declined by 24% in 53˚C treated A. nidulans as compared to controls. Ca^2+ pretreatment also inhibited both the increase of F_O and the decrease of Fv levels induced by high temperature stress. Frnm above results, it was concluded that the inhibition of PS II function in A. nidulans by high temperature stress was resulted from the restrictions of PS II reaction center rather than the malfunction of oxygen evolution complex or the strucutral changes of photosyntheic thyakoid membranes.