Cellulose membrane as a biomaterial: from hydrolysis to depolymerization with electron beam

어미영,범환,조윤주,김성민,이석근 한국생체재료학회 2016 생체재료학회지 Vol.20 No.4

The cellulose membrane (CM) is a major component of plant cell walls and is both a chemically and mechanically stable synthetic polymer with many applications for use in tissue engineering. However, due to its dissolution difficulty, there are no known physiologically relevant or pharmaceutically clinical applications for this polymer. Thus, research is underway on controlled and adjusted forms of cellulose depolymerization. To advance the study of applying CM for tissue engineering, we have suggested new possibilities for electron beam (E-beam) treatment of CM. Treatment of CM with an E-beam can modify physical, chemical, molecular and biological properties, so it can be studied continuously to improve its usefulness and to enhance value. We review clinical applications of CM, cellulose binding domains, cellulose crosslinking proteins, conventional hydrolysis of cellulose, and depolymerization with radiation and focus our experiences with depolymerization of E-beam irradiated CM in this article.

Delaire 개념에 기반한 선천성 구순열의 치료 ; 구순 비근육과 비중격의 정상 해부학적 구조 및 생리기전

유명숙,어미영,이석근,이종호,김성민 대한구순구개열학회 2009 대한구순구개열학회지 Vol.12 No.2

The treatment of cleft lip and palate must be based on a complete knowledge of the anatomy, physiology and growth of the involved deformity, because of not only the appearance but also impaired functions such as phonation, mastication, respiration and lingual posture of the maxillomandibular complex. Delaire has long studied all these aspects, and has published many numbers of articles and constructed a philosophy concerning the significance and interrelationship of the various structures. The results obtained from its application seem to be particularly valid from a clinical point of view, although it has not all been scientifically supported by experimental data. For these reasons, Delaire’s primary unilateral and bilateral cheilorhinoplasty procedures are particulary good, as is his secondary gingivoalveoloplsty procedure during the course of the surgical repair of the hard palate. In order to understand Delaire’s philosophy, it is necessary to consider the normal and pathologic anatomy of the structures involved in the deformity, the role of some structures, such as nasal septum, musculature, and tongue, and some functions, such as dental occlusion or nasal respiration, which play important roles in maxillary and particularly premaxillary growth. Despite of important concept and meanings, Delaire’s philosophy has not been introduced widely to our Korean cleft surgeons yet. So authors will summarize the basic concepts of Delaire’s philosophy according to already published literatures and lectures based on our previous treatment outcomes.

안면부 혈관기형 환자의 수술적 처치

김성민,박정민,어미영,명훈,이종호,최진영 대한구순구개열학회 2010 대한구순구개열학회지 Vol.13 No.2

Vascular malformations (VMs) in the head and neck region are present at birth and grow commensurately with the child, they can result in significant cosmetic problems for the patient, and some may lead to even serious life threatening hemorrhage. Although the molecular mechanisms underlying the formation of these VMs remain unclear, lesions are known to result from abnormal development and morphogenesis. Histologically, there are no evidence of cellular proliferation, but rather progressive dilatation of abnormal channels, which VMs are designated to their prominent channel types such as capillary, venous, lymphatic, arterial, and combined malformations. VMs with an arterial component are rheologically fast-flow, whereas capillary, lymphatic, and venous components are slow-flow. In this article, we review the clinical presentations, diagnosis, and management of VMs of facial regions with author’s embolization and surgical treatment cases.

악교정수술에서 광전자 포인트 마커를 이용한 상악골 위치 변화의 계측 및 계산 방법 연구

박종웅,김성민,어미영,박정민,명훈,이종호,김명진,Park, Jong-Woong,Kim, Soung-Min,Eo, Mi-Young,Park, Jung-Min,Myoung, Hoon,Lee, Jong-Ho,Kim, Myung-Jin 대한악안면성형재건외과학회 2011 Maxillofacial Plastic Reconstructive Surgery Vol.33 No.3

Purpose: To apply a computer assisted navigation system to orthognathic surgery, a simple and efficient measuring algorithm calculation based on affine transformation was designed. A method of improving accuracy and reducing errors in orthognathic surgery by use of an optical tracking camera was studied. Methods: A total of 5 points on one surgical splint were measured and tracked by the Polaris $Vicra^{(R)}$ (Northern Digital Inc Co., Ontario, Canada) optical tracking system in two cases. The first case was to apply the transformation matrix at pre- and postoperative situations, and the second case was to apply an affine transformation only after the postoperative situation. In each situation, the predictive measuring value was changed to the final measuring value via an affine transformation algorithm and the expected coordinates calculated from the model were compared with those of the patient in the operation room. Results: The mean measuring error was $1.027{\pm}0.587$ using the affine transformation at pre- and postoperative situations and the average value after the postoperative situation was $0.928{\pm}0.549$. The farther a coordinate region was from the reference coordinates which constitutes the transform matrixes, the bigger the measuring error was found which was calculated from an affine transformation algorithm. Conclusion: Most difference errors were brought from mainly measuring process and lack of reproducibility, the affine transformation algorithm formula from postoperative measuring values by using of optic tracking system between those of model surgery and those of patient surgery can be selected as minimizing the difference error. To reduce coordinate calculation errors, minimum transformation matrices must be used and reference points which determine an affine transformation must be close to the area where coordinates are measured and calculated, as well as the reference points need to be scattered.

구강 및 안면재건을 위한 광배근 유리피판의 해부학적 고찰

김성민,정영언,어미영,강지영,서미현,김현수,명훈,이종호,Kim, Soung-Min,Jung, Young-Eun,Eo, Mi-Young,Kang, Ji-Young,Seo, Mi-Hyun,Kim, Hyun-Soo,Myoung, Hoon,Lee, Jong-Ho 대한악안면성형재건외과학회 2011 Maxillofacial Plastic Reconstructive Surgery Vol.33 No.6

The latissimus dorsi myocutaneous flap (LDMF) was initially described at the turn of the century by Tansini et al, and latissimus dorsi myocutaneous free flap (LDMFF) was also first described for the coverage of a chronically infected scalp by Maxwell et al. As a pedicled flap, LDMF has been often used for breast reconstruction and for soft tissue replacement near the shoulder and the lower reaches of the head and neck. LDMFF is a flat and broad soft tissue flap with large-caliber thoracodorsal vessels for microvascular anastomosis. A skin paddle of the LDMFF can be more than $20{\times}40$ cm, so very large defects in the oral cavity and outer facial region can be covered by this LDMFF. Other advantages include consistent vascular anatomy, acceptable donor site morbidity and the ability to perform simultaneous flap harvest with tumor resection. For a better understanding of LDMFF as a routine reconstructive procedure in large defects of the oral cavity and facial legion, anatomical findings must be learned and memorized by young doctors during the special curriculum periods for the Korean national board of oral and maxillofacial surgery. This review article discusses the anatomical basis of LDMFF with Korean language.

Delaire 개념에 기반한 선천성 구순열의 치료 II - 기능적 구순비성형술

김성민,서미현,어미영,이석근,명훈,이종호,최진영 대한구순구개열학회 2010 대한구순구개열학회지 Vol.13 No.2

After introduction of Delaire’s basic philosophy, to consider the normal and pathologic anatomy, the role of some structures, such as nasal septum, musculature, and tongue, and some functions, such as dental occlusion or nasal respiration, which play important roles in maxillary and particularly premaxillary growth, on the Korean Journal of cleft lip and palate in 2009, Delaire’s primary functional cheilorhinoplasty (FCR) in the unilateral and bilateral cleft lip patients was summarized and introduced according to already published literatures and lectures. This paper will be the second publishment of Delaire philosophy with Korean language.

혈관 비틀림이 백서 천층하복부 동맥의 초미세문합에 미치는 효과

서미현,김성민,어미영,강지영,명훈,이종호,Seo, Mi-Hyun,Kim, Soung-Min,Eo, Mi-Young,Kang, Ji-Young,Myoung, Hoon,Lee, Jong-Ho 대한악안면성형재건외과학회 2011 Maxillofacial Plastic Reconstructive Surgery Vol.33 No.5

Purpose: The advent of microsurgical technique and instruments, particularly in the field of perforator flap and supermicrosurgery, which have expanded the scope of microsurgery. However, supermicroanastomosis without any compression, tension, or distortions must be achieved to reach successful outcomes. Small-caliber vessels, such as those with an internal diameter less than 0.2 mm, are susceptible to inadvertent twisting of the anastomosis. In this study, using the superficial inferior epigastric artery (SIEA)-based flap model in Sprague-Dawley (SD) rats, we evaluated the acceptable limits of twisting effects on supermicroanastomotic sites. Methods: A total of 20 supermicroanastomoses were performed using the SIEA-based flap model in 10 male SD rats, 10-weeks-of-age, weighing 300~350 g. Rats were divided into five groups of two with four flaps as follows: 1) sham, 2) control group with end to end SIEA arterial supermicroanastomosis, 3) experimental I (EA1) with $90^{\circ}$ twisting, 4) experimental II (EA2) with $180^{\circ}$ twisting, and 5) experimental III (EA3) with $270^{\circ}$ twisting of the supermicroanastomosis. Each SIEA was anastomosed using six 11-0 $Ethilon^{(R)}$ (Ethicon Inc. Co., NJ, USA) stitches except in the sham group where the SIEA was only clamped with Supermicro vascular $clamps^{(R)}$ (S&T, Neuhausen, Switzerland) for 20 minutes. Results: The anastomosed arterial patency showed no remarkable changes according to doppler waveforms measured with a Smardop 45 Doppler System (Hadeco Inc., Kawasaki, Japan). The pulsatility index (PI) was increased at postoperative day 10 in the EA2 and EA3 groups, and the resistance index (RI) showed no statistically significant difference between preoperative and postoperative values at 10 days. Histologic specimens from the EA3 group showed increased tunica media necrosis, convolution of the internal elastic lamina, densely packed platelets, fibrin, and erythrocytes. Flap viability and anastomosed vessel patency were not significantly affected by the degree of arterial twisting in this study, other than in the EA3 group where minor effects on arterial patency of the microanastomoses were encountered. Conclusion: It appears that minor twisting on small caliber arteries, used in supermicroanastomoses, can be tolerated. However, twisting should be avoided as much as possible, and more than $180^{\circ}$ twisting must be prevented in clinical practice.

미더덕 유래 셀룰로오스막의 기본 구조 및 성분 분석에 대한 연구

김성민 ( Soung Min Kim ),어미영 ( Mi Young Eo ),박정민 ( Jung Min Park ),명훈 ( Hoon Myoung ),이종호 ( Jong Ho Lee ),박용일 ( Yong Il Park ),변두진 ( Doo Jin Byun ),이석근 ( Suk Keun Lee ) 한국조직공학과 재생의학회 2010 조직공학과 재생의학 Vol.7 No.2

We have developed cellulose membrane (CM) from the native sea squirt skin since several years ago. The aim of this study is to analyze the basic chemical composition and fiber structure of this CM. To analyze the basic carbohydrate composition and its structures, HPAEC (High Performance Anion-Exchange Chromatography) was used. Elementary analysis by using element analyzer (EA 1110(R), CE instruments Co.) and FE-SEM (Field Emission Scanning Electron Microscope), amino acid analysis by using amino acid analyzer (L-8800(R), Hitachi Co.), transmission analysis with ATR-IR (Attenuated Total Reflection Infrared), surface chemical analysis by ESCA (Electron Spectroscopy for Chemical Analysis), and cellulose fibers analysis by SEM (Scanning Electron Microscopy) were all carried out. Glucose was the major constructive carbohydrate as 95%, and other minor fucose, arabinose and mannose were purified as 1~2 % each. C=O functional group can be also checked in the organic and nonorganic synthetic materials, and surface chemical bonding energy of each carbon and oxygen was confirmed through C1s and O1s spectra. From these basic analysis, CM has pure carbohydrate polymer structures consisted of rigid outer surface and delicate inner surface, which will be able to be developed as the tissue regenerative barrier membrane in the medical scope.

전자빔을 조사한 7대 3 비율의 수산화인회석과 인산삼칼슘 혼합무기물의 골재생 효과

김성민 ( Soung Min Kim ),어미영 ( Mi Young Eo ),강지영 ( Ji Young Kang ),명훈 ( Hoon Myoung ),최은경 ( Eun Kyung Choi ),이석근 ( Suk Keun Lee ),이병철 ( Byung Cheol Lee ),이종호 ( Jong Ho Lee ) 한국조직공학·재생의학회 2012 조직공학과 재생의학 Vol.9 No.1s

The aim of this study is to evaluate the effect and potential of electron beam (E-beam) irradiation treatment to the new bone formation in the rat calvarial bony defects by using of 7 to 3 ratios of hydroxyapatite (HA, Bongros(R), Bio@ Co., KOREA) and tricalcium phosphate (β-TCP, Sigma-Aldrich Co., USA) mixed with type I collagen (Rat tail, BD Biosciences Co., Sweden). We used 1.0 MeV linear accelerator and 2.0 MeV superconductive linear accelerator (power 100 KW, pressure 115 kPa, temperature - 30~120oC, sensor sensitivity 0.1~1.2 mV/kPa, generating power sensitivity 44.75 mV/kPa, supply voltage 5±0.25 V) with different irradiation dose, such as 1, 30, 60 kGy. Bilateral, standardized transosseous circular calvarial defects, 6.0 mm in diameter, were made in a total of twenty-one, 8-weeks-old, male Sprague-Dawley rats, weighing 270-300 g. In six different experimental groups, the defect of every three rats was filled with E-beam irradiated bony mixtures, which different energy and dose, and that of rats without E-beam irradiated bony mixtures can be compared with our previous results. The other three rats were used as sham group. Rats were sacrificed after 4, 8, and 16 weeks, and grafted healing specimens were studied by clinical and radiographic (standardized plain film, Kodak Co., USA) findings, histomorphologic staining with hematoxylin and eosin (H & E), FE-SEM (field-emission scanning electron microscopy), and TEM (transmission electron microscopy) findings. The large particular size of HA was changed to small particles after E-beam irradiation, to which small particle of TCP was engaged to small sixed HA with organic collagen components in SEM findings. Abundant endothelial cells with pericytes were found around inner portions of grafted healing bone and many couplings of osteoblast with osteoclast in TEM findings were found in every experimental groups.

New approach for the treatment of osteoradionecrosis with pentoxifylline and tocopherol

범환,김성민,조윤주,어미영,이석근,우경미 한국생체재료학회 2014 생체재료학회지 Vol.18 No.4

Osteoradionecrosis (ORN) of the jaw is a significant complication of radiotherapy for oral cavity cancer. In addition to antibiotic medication, treatment options such as hyperbaric oxygen therapy, surgical approaches, and combined therapy with pentoxifylline and tocopherol have been recently introduced. In this review article, we will discuss the definition and classifications of osteoradionecrosis, its etiology and pathophysiology, previous treatment options, oral and maxillofacial complications of radiotherapy, basic information on pentoxifylline and tocopherol, recent reports of pentoxifylline and tocopherol combined therapy, and, finally, ORN-induced animal models and future approaches.