군에서 발생하는 사고환자에 대한 조사 연구

안창숙,이시영,진은자 국군간호사관학교 군진간호연구소 1988 군진간호연구 Vol.8 No.-

NCS 커피 교육프로그램이 교육만족과 실무전이에 미치는 영향

안창숙,전재균 (사)한국관광레저학회 2023 관광레저연구 Vol.35 No.-

In the domestic coffee market, the amount of import and consumption has increased even during the COVID-19 pandemic. In order to cope with the size of the coffee market and the diversity in terms of consumption, the country has developed a coffee National Competency Standards(NCS) that standardizes job competency required in industrial sites. The purpose of this study is to empirically investigate how the NCS coffee education program affects education satisfaction and transfer of training, and whether the effect of education satisfaction on transfer of training is significant. The results of the empirical analysis are as follows. First, among the components of the NCS coffee education program, education method, education contents, education instructors had a positive effect on education satisfaction, but the education environment had no significant effect on education satisfaction. Second, among the components of the NCS coffee education program, only the education contents and the education instructor had a positive effect on the transfer of training. Third, education satisfaction was found to have a positive effect on the transfer of training. Finally, this study intends to present a plan to improve NCS coffee education satisfaction and transfer of training in terms of education programs.

NCS 커피 교육프로그램이 교육만족과 실무전이에 미치는 영향

안창숙(An, Chang-Sook),전재균(Jun, Jae-Kyoon) 한국관광레저학회 2023 한국관광레저학회 학술발표대회 Vol.2023 No.2

In the domestic coffee market, imports and consumption continued to increase even during the spread of COVID-19. Based on this, the number of coffee-related industries and coffee-related workers is also expanding. In line with the expansion and change of the coffee industry, the country has developed the National Competency Standards, which standardized the job of coffee, since 2013. We would like to confirm whether coffee education is currently solving the problems of the coffee industry through NCS, how the NCS education is satisfied, and whether NCS education is possible to cultivate practical human resources required in the actual field. For this purpose, this survey was conducted on the students of universities and academies who experienced the NCS coffee education, and the final 204 questionnaires were empirically analyzed using the SPSS 27.0 statistical program. The results of the empirical analysis are as follows. First, among the components of the NCS coffee education program, educational method, educational contents, educational instructors had a positive effect on educational satisfaction, but the educational environment had no significant effect on educational satisfaction, so hypothesis 1 was partially adopted. Second, educational satisfaction was found to have a positive effect on the transfer of training, so hypothesis 2 was adopted.

군 간염환자의 불안과 간호 요구간의 상관 관계에 대한 연구

안창숙 ( Chang Sook An ),김은희 ( Eun Hee Kim ) 국군간호사관학교 군진간호연구소 1987 군진간호연구 Vol.7 No.-

전문 자격별 간호장교의 직무 만족도 조사

함영희 ( Young Hee Ham ),안창숙 ( Chang Sook An ),김은희 ( Eun Hee Kim ) 국군간호사관학교 군진간호연구소 1989 군진간호연구 Vol.9 No.-

Silencing of NbNAP1 Encoding a Plastidic SufB-like Protein Affects Chloroplast Development in Nicotiana benthamiana

Hyun-Sook Pai,안창숙,Jeong Hee Lee 한국분자세포생물학회 2005 Molecules and cells Vol.20 No.1

It was previously shown that AtNAP1 is a plastidic SufB protein involved in Fe-S cluster assembly in Arabidopsis. In this study, we investigated the effects of depleting SufB protein from plant cells using virusinduced gene silencing (VIGS). VIGS of NbNAP1 encoding a Nicotiana benthamiana homolog of AtNAP1 resulted in a leaf yellowing phenotype. NbNAP1 was expressed ubiquitously in plant tissues with the highest level in roots. A GFP fusion protein of the N-terminal region (M1-V103) of NbNAP1 was targeted to chloroplasts. Depletion of NbNAP1 resulted in reduced numbers of chloroplasts of reduced size. Mitochondria also seemed to be affected. Despite the reduced number and size of the chloroplasts in the NbNAP1 VIGS lines, the expression of many nuclear genes encoding chloroplast- targeted proteins and chlorophyll biosynthesis genes remained unchanged.

과립구대식세포 증식인자가 사람 영양막세포의 유전자 발현에 미치는 영향

이헌열 ( Hun Yul Lee ),김상수 ( Sang Soo Kim ),안창숙 ( Chang Suk Ahn ),박영주 ( Young Joo Park ),전진현 ( Jin Hyun Jun ),송행석 ( Haeng Suk Song ),문혜성 ( Hye Sung Moon ),박은주 ( Eun Joo Park ),홍서유 ( Seo You Hong ),박원일 ( 대한산부인과학회 2010 Obstetrics & Gynecology Science Vol.53 No.10

목적: 포유동물의 생식과정에서 Granulocyte-macrophage colony-stimulating factor (GM-CSF)가 배아의 침윤성을 증가시킨다는 사실은 알려졌으나 착상 이후와 태반형성에서의 작용은 알려진 바가 거의 없다. 본 연구는 GM-CSF가 임신초기 사람의 영양막세포에서도 침윤성을 증가시키는 작용이 있는 지를 확인하는 것이 목적이다. 연구 방법: 임신초기 소파술을 통해 얻은 사람의 영양막세포를 배양한 후 GM-CSF 처리군과 대조군의 유전자 발현 차이를 microarray를 통하여 비교하였고 차이가 있는 유전자 중 착상과 관련된 유전자의 발현을 real-time RT-PCR로 확인하였다. 결과: Microarray 결과 GM-CSF 처리군에서 2배 이상 증가한 유전자는 468개, 2배 이상 감소한 유전자는 40개였다. 이 유전자를 생물학적 pathway에 따라 분석한 결과 actin cytoskeleton 및 focal adhesion에 관여된 유전자가 많았다. Actin cytoskeleton 및 focal adhesion에 관여된 유전자 중 annexin A2, thymosin-like 3, vimentin, myogenin, ACK1, tensin1의 발현을 real-time RT-PCR로 확인하였다. 그 결과 GM-CSF에 의해 vimentin과 ACK1의 발현은 증가되었고 tensin1의 발현은 감소되었다. 결론: GM-SF는 사람의 초기 임신 영양막세포에서 vimentin과 ACK1의 발현을 증가시키고 tensin1을 감소시킴으로써 focal adhesion을 증가시킨다고 생각된다. Objective: Granulocyte-macrophage colony-stimulating factor (GM-CSF) facilitates mammalian embryonic development and implantation. However, its biological function after implantation is not elucidated. The aim of this study is to assess the changes of gene expression by GM-CSF in human trophoblast obtained in early pregnancy. Methods: Human trophoblast obtained in early pregnancy was cultured with or without GM-CSF. The difference of gene expression was evaluated with microarray and selected genes were reevaluated with real-time reverse transcription-polymerase chain reaction (RT-PCR). Results: Microarray analysis revealed that the expressions of 468 genes were increased while those of 40 genes were decreased by GM-CSF. These genes were evaluated according to the known biologic pathways. The regulation of actin cytoskeleton and focal adhesion pathways were mostly influenced by GM-CSF. Annexin A2, thymosin-like 3, vimentin, myogenin, ACK1, and tensin1 genes were selected for real-time RT-PCR. The increased expressions of of vimentin and ACK1, and decreased expressions of tensin1 were confirmed by real-time RT-PCR. Conclusion: GM-CSF activates focal adhesion pathway in human trophoblast by increasing the expression of vimentin and ACK1, and decreasing the expression of tensin1.

배아 이식에서 임신율에 영향을 주는 요소들: 다변인 분석

홍유경 ( Yu Kyung Hong ),서은지 ( Eun Jee Seo ),안창숙 ( Chang Sook An ),구지선 ( Jee Sun Gu ),차병헌 ( Byung Hun Cha ),박은주 ( Eun Joo Park ),박원일 ( Won Il Park ),이진용 ( Jin Yong Lee ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.11

목적: 배아 이식 단계는 보조 생식 시술의 성공에 중요한 부분을 차지해 왔지만, 그에 비해 그동안 배아 이식 기술에 대한 충분한 연구 평가가 이루어지지 않았었다. 본 연구의 목적은 이식 시술의 다양한 요인들이 시술 성공률에 미치는 영향에 대해 조사하는 것이다. 연구 방법: 이식관의 종류 (부드러운 혹은 단단한), 이식관에 피가 묻었는지의 여부, 이식의 난이도 (쉬움, 중등도, 힘듦), 그리고 시술 의사를 변수로 하여 체외수정의 결과, 즉 임상적 임신율 (초음파로 태아 심박동 확인)과 출산 임신율의 차이를 분석하였다. 그 외 환자의 나이, 총 획득 난자 수, 자궁내막 두께, 이식한 배아 중 최상급 배아의 질 등을 결과에 영향을 미칠 수 있는 매개변수로 선정하였다. 단일 변인 분석은 카이자승 검정을, 다변인 분석은 로지스틱 회귀 분석을 사용하여 결과를 얻었다. 결과: 배아 이식 시술의 여러 요인들 중에 임신율과 생존 출산율 모두에 유의한 영향을 미치는 요인은 시술 난이도로 나타났다. 쉬운 이식과 중등도 이식 사이의 시술 성공률은 차이가 있었으나, 중등도와 힘든 이식의 차이는 없었다. 또한 이식관에 피가 묻었는지의 여부가 생존 출산율에 유의한 영향을 미치는 것으로 나타났다. 그 외 이식관의 종류와 시술 의사는 임신율, 출산율에 의미 없는 것으로 나타났다. 결론: 보조 생식 시술의 결과에 영향을 미치는 중요한 요인으로 이식관에 피가 묻었는지의 여부와 배아 이식 시술의 난이도를 들 수 있다. Objective: Embryo transfer is a critical step in assisted reproduction. However, the different aspects of the procedure were not fully estimated. The aim of this study is to evaluate the impact of each aspect in the procedure to success of pregnancy. Methods: Medical records of 525 fresh IVF/ICSI cycles were reviewed retrospectively. All embryo transfers were performed under ultrasonography guidance and after removal of cervical mucus. Assessed primary variables are difficulty of procedure (easy, moderate, and difficult), presence of blood in the catheter, types of catheter (soft or hard) and physician factor, and confounding variables are age of patient, total number of oocytes, number of transferred embryos, the highest grade of embryo, endometrial thickness. Measured outcomes were clinical pregnancy (visible fetal heart beat by ultrasonography) and live-birth rate. Univariate analysis and multivariate analysis by logistic regression test were used. Results: Among the variables of embryo transfer procedure, the difficulty of procedure was the only significant factor influencing both clinical pregnancy (OR: 0.402, 95% CI: 0.205-0.789, easy vs. moderately difficult) and live birth rate (OR: 0.380, 95% CI: 0.178-0.841, easy vs. moderately difficult). The difference between moderately difficult and very difficult procedure was not significant. Presence of blood was significant for live-birth rate (OR: 0.512, 95% CI: 0.267-0.980). Types of catheter and physician factor were not significant factors. Conclusion: The difficulty of the transfer procedure and presence of blood in catheter are significant factors influencing ART outcome.