EB Method를 이용한 고속도로 무인과속단속카메라의 사고감소 효과분석

심대용,김동규(Kim, Dong-Gyu),박신형(Park, Sin-Hyeong),고승영 대한교통학회 2012 대한교통학회 학술대회지 Vol.66 No.-

Discontinuous Percoll Gradients Enrich X-Bearing Porcine Sperms and Female Embryos

심대용,유성진,강한승,유정민,이채관,강성구 한국발생생물학회 2001 발생과 생식 Vol.5 No.1

X, Y정자를 분리하여(sexing techlology)필요한 성을 조절한 배아의 연구가 아직 실용화되지 않고 있다 본 연구는 이에 대한 기초연구로서 돼지의 X 정자와 Y정자를 분리하기 위하여 불연속 percoll농도구배를 제조한 후 상층에 정액을 분주하여 120g에서 20분간 원심분리 하였다. 분리된 각 층의 정자를 회수(710 sperms/ml)하여 genomic DNA를 추출한후, PCR 방법을 이용하여 Y 염색체의 성 결정 유전자(TDF)인 SR Predetermination of sex in livestock of offpring is in great demand and is of critical importance to providing for the most efficient production of the animal ariculture. Such a sexing techlology would also enhance the economy of conventional artificial insemination(AI) and aid the porcine industry. The purpose of this study was to evaluate the efficiency of enriching X-bearing porcine sperm using discontinuous percoll gradients and PCR mefhod. Semen was collected from mature boars of proven fertility center (AI center KimHae). Sperm was leaded on the isotonic discontinuous percoll gradient and then it was centrifuged at 120 g for 20 minutes. After centrifugation, sperm included in each fraction were recovered (710 sperms/ml) and then sperm genomic DNA was extractedfor the PCR. SRY gene was used to evaluate the ratio between X and Y sperm in the separated fractions. Ju viro ffrtilization wascarried out by adding the unseparated sperm (control) or separated (experimental poop) to the matured oocytes in TCM-199. Embryos for sex determination were obtained at 2 cell stage and then was used for SRY gene amplification. After centrifugation of discontinuous percoll gradient, the most motile sperm was obtained at 95% fiaction (94.4% 5.1%, p < 0.01). The PCR analysis evaluated that 30%, 50% and 65% fractions were Y sperm rich, whereas 80% and 95% fractions were X sperm rich. PCR analysis with each porcineembryo showed that 33.3% of control and 66.7% of experimental group were determined as female embryos. In conclusion, in vitro matured oocytes inseminated with sperms (95% fraction) prepared by percoll gradient centrifugation showed high fertilization rates and female embryos than control sperms.

도시부도로의 돌발상황 판정 및 영향권 설정에 관한 연구

심대용,고승영,하동익 한국ITS학회 2016 한국ITS학회 학술대회 Vol.2016 No.04

『이자서절요(李子書節要)』의 편찬목적과 체제

심대용 동양예학회 2017 東洋 禮學 Vol.36 No.-

로운(老耘) 윤동석(尹東晳)의 생애와 저술에 관하여

심대용 동양예학회 2015 東洋 禮學 Vol.34 No.-

우리나라 정신건강의학과 의사의 직무스트레스 및 직업만족도 예비연구

심대용,최종혁,견영기 대한신경정신의학회 2022 신경정신의학 Vol.61 No.1

Objectives Physicians experience high occupational stress due to heavy workload, responsibility and stressful situations in the medical field. Although occupational stress decreases productivity and increases medical accidents, occupational stress on Korean psychiatrists is not well studied. In this study, we investigated the occupational stress and career satisfaction of Korean psychiatrists. Methods Electronic surveys were conducted via e-mail, and a total of 266 psychiatrists fully answered the surveys. The surveys consisted of the Korean Occupational Stress Scale (KOSS), career satisfaction and stress inventory for the health professional. Results The mean KOSS score of Korean psychiatrists was lower than the score of other Korean professions (43.99 vs. 46.03). There are significant differences in the sub-items of KOSS, depending on the lifestyle, the career and the hospital on duty. Psychiatric residents scored significantly higher in job control and occupational climate than psychiatrists. On the other hand, workplace relationships and job insecurity scored significantly higher in psychiatrists. Psychiatrists working in general and university hospitals had higher stress in job demand than psychiatrists working at private psychiatric hospitals. Job control and lack of reward scores were high in psychiatrists working in national psychiatric hospitals, and 21.8% of psychiatrists answered that they would not become a psychiatrist again. Conclusion This study showed the overall job stress level of psychiatrists in Korea, and they were experiencing high job stress in different areas depending on factors, such as position, working environment and living habits.

[박사학위논문소개] 신뢰도를 고려한 대중교통 노선평가 기준 개발

심대용(SIM, Dae Yong) 대한교통학회 2020 교통 기술과 정책 Vol.17 No.2

대장암과 동반된 double primary cancer 14례 보고

황대용,방호윤,김병기,김종훈,박상윤,이의돈,박종호,심윤상,문난모,이승숙,조경자,강신광 대한외과학회 1996 대한외과학회 학술대회 초록집 Vol.1996 No.11

마취약이 돼지정자의 운동성과 수정에 미치는 영향

배진현,심대용,강한승,강성구 인제대학교 2002 仁濟論叢 Vol.17 No.1

정자의 운동성은 수정에 있어서 필수적인 요소이며 이러한 운동성은 사정직후 이온채널의 열림과 ATPase의 활성에 의해 획득된다. 이 연구의 목적은 이온 채널을 봉쇄하는 마취제에 돼지의 정자를 노출시켜 일시적으로 ATPase의 활성을 억제하여 정자의 운동성을 감소시킴으로서 정자보존의 효율을 높이고자 하는데 있다. 돼지의 정자는 A. I.(Artificial Insemination) Center에서 공급받았으며 ㎖당 20 × 106개 이상의 운동성 있는 정자를 사용하였다. 마취제에 정자를 각각 1시간과 3시간 노출시킨 후 운동성을 확인하고, 이를 다시 마취제를 제거 한 후 난자와 인공수정을 시행하였다. Lidoacine과 tricaine에 노출된 정자는 운동성을 가진 정자의 수가 대조군에 비해 감소하였으며 이를 다시 희석액에 세척하여 마취제를 제거하여도 운동성을 가진 정자의 수는 대조군의 수만큼 회복되어지지 않았다. 또한 lidoacine과 tricaine에 노출된 정자의 마취제를 제거한 다음 인공수정을 시행한 결과 대조군에 비해 수정율이 감소되었다. Propofol의 경우에 있어서는 운동성을 가진 정자의 수가 대조군에 비해 감소하지 않음에도 불구하고 propofol에 노출된 정자로 인공수정을 시행한 결과 수정율이 감소되었다. 이상의 결과 lidoacine과 tricaine은 이온 채널을 봉쇄함으로서 정자의 운동성을 제한하고 제한된 정자의 운동성은 마취제를 제거하더라도 다시 원래의 수준으로 회복되어지지 않는 것으로 보여진다. Propofol의 경우에 있어서는 직접적으로 운동성을 제한하지 않았으나 수정시 첨체반응을 유도하는 GABA 수용체를 차단하여 수정에 부정적인 영향을 미치는 것으로 보여진다. The motility of sperm is essential in fertilization. The motility is obtained by the activation of ATPase. The purpose of this study is the suppression of motillty in sperm is increased by inhibition in the activation of ATPase, so we exposed sperm to an anesthetic. Porcine sperm was supplied by Kim-hae A.I.(Artificial Insemination) center. And porcine sperm samples with a sperm density greater than or equal 20 × 106/ml and progressive motility. Observation was performed on all samples at 1 and 3 hour(s) after the addition of anesthetic drugs, and then IVF(In Vitro Fertilization) was performed after were an anesthetic drugs were washed. Compared with control, lidocaine and tricaine treated sperm motile rates were decreased. After anesthetic drugs were removed, the motility of porcine sperm was not recovered. Also fertilization rate was decreased as compared with control. Compared with control, motile rate was not changed but fertilization rate was decreased in propofol treated group. These result suggested that sperm, temporary exposed to lidocaine or tricaine, decreased motility and fertilization rate. And propofol did not inhibite motility, but it has negative effect on fertilization because of the influence of propofol for acrosome reaction.

대학 축구선수의 무산소성 예비량과 무산소성 운동능력의 상관성

정진원(Jin Won Jeong),심대용(Dae Yong Sim) 한국운동영양학회 2000 Physical Activity and Nutrition (Phys Act Nutr) Vol.4 No.1

N/A This study employed 11 university soccer players for assessing anaerobic capacities related to ATP-PC and/or lactate system. It was investigated correlation among anaerobic capacities from Critical Power test, from Wingate test, and from Isokinetic test. Also, the study investigated regression formula which could expect anaerobic reserve(AR) from variables measured in Wingate and Isokinetic test. There were significant but intermediate correlation between AR and WIN5, (r= .62, P< .05) and WIN30(r= .64, P< .05). There was no statistically significant but intermediate correlation between AR and WIN10 (r= .52, P> .05). The study used stepwise method for multiple regression by Wingate variables to produce AR. The result showed that WIN30 had significant effect and indicated regression formula (Y= 1.3323(WIN30)-14737.758) to explain 41.2% variation of AR. Stepwise method was done for multiple regression on Isokinetic variables to produce AR`s inferred possibility by extensors TW of 60˚ /sec as regression (Y= 10.6737 (TW)+2083.9403) explained 42.9% variation of AR. In the correlation between measured variables of Wingate and Isokinetic extensors there was no significance of intermediate correlation in 60˚ /sec PT and WIN5(r= .53, P> .05) and in TW and WIN5(r= .56 P< .05) statistically. Also, There was significance of intermediate correlation in PT and WIN10 (r= .65, P< .05), no significance in TW and WIN10 (r= .57, P> .05), in PT and WIN30(r= .58), and no significance in TW and WIN30(r= .46, P> .05). In the correlation between measured variables of Wingate and Isokinetic extensors, there was significance of intermediate correlation in 180˚ /sec PT and WIN10 (r= .69, P< .05) and significant correlation in Isokintic 180˚ /sec PT and WIN30(r= .65, P< .05). The data suggested that anaerobic reserve assess true anaerobic ability not including the energy component of anaerobic glycolyses. Also, the study provides preliminary data on the anaerobic abilities in university soccer players as assessed by the Critical Power test, Wingate test, and Isokinetic test.