미니돼지에서 ^99mTc-Tin colloid와 ^99mTc-DISIDA를 사용한 간신티그라피의 비교 연구

심경미,김세은,이원국,궁성수,배춘식,이재영,최석화,한호재,강성수,박수현,Shim, Kyung-Mi,Kim, Se-Eun,Lee, Won-Guk,Koong, Sung-Soo,Bae, Chun-Sik,Lee, Jae-Yeong,Choi, Seok-Hwa,Han, Ho-Jae,Kang, Seong-Soo,Park, Soo-Hyun 한국생명과학회 2006 생명과학회지 Vol.16 No.6

동물 모델에서의 간기능의 비침습적 평가는 하나의 과제로 남아있다. 간신티그라피는 간의 크기 및 모양의 변화에 대한 정보를 제공할 뿐 아니라 간의 전체적인 기능을 파악할 수 있게 해 주며 간이식의 주요 합병증인 간염, 거부반응 및 담도계 합병증의 진단에도 편리하게 사용될 수 있다. 이에 본 연구에서는 미니돼지의 간 기능을 평가하기 위해 사람에서 주로 사용되는 방사성의약품인 $^{99m}Tc-Tin$ colloid와 $^{99m}Tc-DISIDA$를 이용하여 간 및 간담도 신티그라피를 시행하였다. 그 결과, 사람의 간신티그라피와 달리 미니돼지에서는 $^{99m}Tc-Tin$ colloid가 폐, 간, 위벽 및 신장에 섭취되고 폐와 간의 영상이 겹쳐져 간의 관심영역을 설정할 수 없었다. 그러나 $^{99m}Tc-DISIDA$를 사용한 간담도 신티그라피에서는 심장, 폐, 간, 담낭 및 십이지장으로의 연속적 방사능섭취를 관찰할 수 있었다. 따라서 미니돼지에서의 $^{99m}Tc-Tin$ colloid의 사용은 비특이적 결합력으로 인해 간 기능 평가를 위한 영상에는 적합하지 않으나 $^{99m}Tc-DISIDA$는 간담도 기능을 파악하는데 있어 좋은 수단이 될 수 있을 것으로 생각된다. Non-invasive evaluation of liver function in animal models remains a challenge. Hepatoscintigraphy provides information about changes in liver size and shape, and enables to understand general liver function. Futhermore it is readily used to diagnosis complications of liver transplantation like hepatitis, rejections and biliary complications. In this study, we investigated the usefulness of evaluating the liver function in miniature pigs with $^{99m}Tc-Tin$ colloid and $^{99m}Tc-DISIDA$ which are the most commonly used radiopharmaceuticals in human medicine. In result, $^{99m}Tc-Tin$ colloid was uptaked in lung, liver, gastric wall and kidney in miniature pigs. And $^{99m}Tc-DISIDA$ showed continuous uptake images of heart, lung, liver, gallbladder and duodenum, and it was similar to human's. Therefore we could conclude $^{99m}Tc-Tin$ colloid would not be suitable for evaluating hepatic function because of it's nonspecific affinity, however $^{99m}Tc-DISIDA$ scintigraphy would be an effective method for detecting hepatobiliary function in miniature pigs.

랫드 두개골 결손부에서 단삼 수용성 추출물의 골형성 효과

심경미,김세은,강성수,Shim, Kyung Mi,Kim, Se Eun,Kang, Seong Soo 대한수의학회 2010 大韓獸醫學會誌 Vol.50 No.3

The purpose of this study was to evaluate the osteogenic capacity of water extract of danshen (Salvia miltiorrhiza Bunge). We have established in rat critical-sized calvarial defect model using the combination with collagen scaffold and danshen hydrophilic extract. All rats were extinguished at 8 weeks after bone graft surgery, and the bone regeneration ability of bone grafting sides was evaluated by plain radiography and micro-CT. These results revealed water extract of danshen had the potential to promote osteogenesis especially continuous oral administration with local treatment compared to one-shot local treatment. This compound may provide a new alternative agent for growth factors to promote bone healing and bone regeneration. In conclusion, these results suggest that danshen hydrophilic extract have the potential to promote osteogenesis in bone defects. Further studies about fusion technology with salvianolic acid B, peptides, growth factors, and scaffolds using of the combination of tissue engineering, cell engineering and mechanical engineering are needed.

초등학생의 영어학습에 영향을 끼치는 변인연구

심경미(Kyung-Mi Shim),이길영(Kil-Ryoung Lee) 한국외국어대학교 영미연구소 2007 영미연구 Vol.17 No.-

This paper is for investigating how various childhood backgrounds and approaches taken by elementary school children to learning English affect acquisition of skills in listening, speaking, writing and reading. Furthermore, it investigated what the variables are that cause the differences in terms of the students' level of linguistic competence. Written survey questions were asked of 300 sixth graders from three different elementary schools to assess level of their achievement. Employed for this survey was a written questionnaire containing 42 items and each listening/speaking/writing/reading comprehension test. The data obtained were analyzed according to SAS 8.01 statistical package program. Based on this data analysis, the students surveyed were divided into three groups; upper (25%), middle (50%), and lower (25%). Among the 42 variables, the 29 ordinal scale variables were subjected to regression analysis, and the result was 7 major factors which the researchers named as follows; (1) speaking English at home, (2) family environment, (3) classroom activities, (4) motivation to learn English, (5) amount of time and frequency of study, (6) psychological factor, (7) socio-cultural factor. It was revealed in the data that students who proved competent within the 5% range in one of four language functions were also competent in the other categories.

반복하중재하에 의한 표층처리지반의 지지력특성

심경미(Shim, Kyoung-Mi),곽노경(Kwak, No-Kyung),양기석(Yang, Kee-Suk),이송(Lee, Song) 한국구조물진단유지관리학회 2010 한국구조물진단학회 학술발표회논문집 Vol.2010 No.2

Dredged soils by pump dredging vessel applied to large-scale reclamation projects have high water content on totally disturbed state. So, construction equipment even can not enter dredged site. However the study of surface ground reinforcing method is supposed to be considered preferentially is not satisfied and also doesn't contemplate plastic flow because of repetitive drive of construction equipment. Also, Terzaghi's bearing-capacity equation and Yamanouchi's suggestion have been used to design the surface reinforcement, but most engineers depend on their experience and cases constructed before because of dispersed variables and inappropriate bearing-capacity factors. Hence, plate load test and repetitive plate load test are performed in the field which is reinforced with geotextile(30ton/m), geogrid(20ton/m, 10ton/m) and bamboo(0.4m?0.4m). The object of this study is that bearing capacity and behaviour of surface ground are evaluated and compared to each reinforcement form test results.

토착미생물 발효물인 CS682의 급여가 닭과 돼지의 생산성에 미치는 영향

Hu Kyung Kim(김휴경),Se Eun Kim(김세은),Kyung Mi Shim(심경미),Jong-Choon Kim(김종춘),Chun-Sik Bae(배춘식),Seok Hwa Choi(최석화),Seong Soo Kang(강성수) 한국생명과학회 2010 생명과학회지 Vol.20 No.2

CS682의 육계 및 이유자돈에서 사료첨가제로서의 기능성을 검정하기 위해 병아리에 28일간 0.1%, 1%의 농도로 사료에 첨가, 급여하여 질병예방기능, 성장기능, 사료효율기능 및 안전성 평가를 실시한 결과, 위장관계 기능장애를 일으키지 않고, 생체대사에 영향을 미치지 않으면서 사료효율을 높이는 것으로 나타났다. CS682 투여 후 14일째에는 각 군 간에 증체량 및 사료효율에서 차이가 미미하였으나, 28일째에는 대조군에 비해 0.1%군은 115.64, 1%군은 122.49%의 사료효율을 보여 각 군 간에 큰 차이를 나타내었다. 그리고 28일째 각 군에서 채혈한 혈액의 기본 혈구검사를 실시한 결과 각 군 간에 유의적인 차이를 보이지 않고 모두 정상 범위를 나타낸 것으로 보아 CS682는 생체대사에 영향을 미치지 않는 것으로 보인다. 또한 이유자돈에서 28일간 0.1%의 농도로 사료에 첨가, 급여하여 질병예방기능, 성장기능, 사료효율기능 및 안전성 평가를 실시한 결과에서 임상적으로 특이한 부작용 없이 대조군에 비하여 높은 사료효율을 나타냈다. 결론적으로 육계와 이유자돈에서 CS682의 사료첨가는 생체대사에 영향을 미치지 않고, 소화기 이상 증상을 유발하지도 않으면서 사료효율을 효과적으로 증가시키는 것으로 나타났다. 본 결과를 토대로 육계 및 이유자돈에서 안전하게 증체율 및 사료효율을 증가시킬 목적으로 CS682를 사료에 첨가하는 것이 효과적일 것으로 생각된다. Antibiotics in veterinary medicine have been used to treat disease, promote growth rate and improve feed efficiency. However, alternative sources are needed because of of bacterial resistance to antibiotics and residues of antibiotics. The present study was carried out to evaluate the effect of CS682, a fermentation product of Korean soil bacteria, on safety, growth rate and feed efficiency, and to evaluate its value as an alternative for antibiotics used as a feed additive. Two dosages of CS682 (0.1% and 1%) were fed to chickens for 28 days. The results showed that, when compared to chickens in the control group, growth and feed efficiency was improved. Also, mortality, hematology, general clinical signs and necropsy were examined. Chickens in the treatment groups showed no adverse effects. A total of 72 weaning pigs were used to confirm the effects of CS682 at one dose level (0.1%) regarding feed efficiency. Supplementation with 0.1% CS682 also resulted in improved weight gain and feed efficiency ratio. Based on these results, CS682 may be effective in improving feed efficiency safely as a feed additive.

광주지역 길고양이 구강질환의 임상적 특성

명보영,김세은,심경미,유지원,김성호,문창종,배춘식,최석화,강성수,박현정,Myung, Bo Young,Kim, Se Eun,Shim, Kyung Mi,Ryu, Ji Won,Kim, Sung Ho,Moon, Changjong,Bae, Chun-Sik,Choi, Seok-Hwa,Kang, Seong Soo,Park, Hyunjeong 대한수의학회 2010 大韓獸醫學會誌 Vol.50 No.4

Demand on specialized feline dentistry has increased over the years, partly due to growing feline population in Korea. Despite the notable increase in the population, not much researches has been conducted on oral disease for stray cats. Thus this research was conducted on 81 adult stray cats from Gwangju in Korea (42 males and 39 females) with average weight of 3.4 kg and also focused on occurrence of periodontal disease (plaque and calculus), feline odontoclastic resorptive lesion (FORL), stomatitis and tooth anomaly. Formation of dental plaque and calculus were abundant on maxilla than mandible, especially distributed much on 4th premolar teeth and 1st molar teeth. After thorough examination of oral cavity, 33 stray cats (40.7%) were found to have oral disease. Eighteen stray cats (22.2%) had tooth fracture which was evident on cuspid and missing teeth were present on 2nd premolar tooth of maxilla and 2nd incisor of mandible amongst 20 stray cats (24.7%). FORL was present on cuspids and 2nd premolar tooth of maxilla among 10 stray cats (12.3%). Stomatitis was involved with FORL, missing tooth, and tooth fracture. Therefore, it was determined that stomatitis, FORL, and periodontal disease were closely associated.

쉴드 터널 세그먼트 라이닝 설계에서 빔-스프링 구조 모델이 단면력에 미치는 영향

김홍문,김현수,심경미,안성율,Kim, Hong-moon,Kim, Hyun-su,Shim, Kyung-mi,Ahn, Sung-youll 한국터널지하공간학회 2017 한국터널지하공간학회논문집 Vol.19 No.2

쉴드터널의 세그먼트 라이닝 단면 설계는 일반적으로 빔-스프링 모델을 사용하여 이루어지며, 산정되는 단면력은 모델구성요소인 작용하중, 지반반력계수, 세그먼트 조인트 위치 및 강성 등의 영향을 받는다. 국내 쉴드터널 세그먼트라이닝의 설계는 해외에서 사용되고 있는 구조해석 모델과 이음부 강성을 주로 이용하고 있으며, 기존 설계에서 사용하고 있는 구조해석 모델이나 조인트 강성을 적합여부 검토 없이 관행적으로 사용해오고 있다. 본 연구에서는 쉴드터널 세그먼트 라이닝의 부재력을 산정하기 위해 필요한 지반반력계수, 모델링 방법 등 빔-스프링 모델의 기본 구성요소들에 대한 비교 검토를 통하여 세그먼트 라이닝 설계법을 구성하는 기본 요소들의 적용방법을 제안하였다. The segment lining design for shield tunnel is generally carried out by using the beam-spring model and the induced member forces from the model are strongly influenced by the components of the model such as imposed load, coefficient of subgrade reaction, location of segment joint and its stiffness. The structural models and stiffness of its connection part found used in abroad design cases is usually obtained as it is for the domestic design of segment of shield tunnel. Those models and stiffness in existing design cases are conventionally applied to a new tunnel design without any suitability review for the project. In this study, the application method of base components of the model such as the coefficient of subgrade reaction and modelling method to the segment lining design was suggested by carrying out the comparative study of the base elements for the member forces estimation of segment lining of shield tunnel.

Gemifloxacin이 미성숙 랫드의 아킬레스건에 미치는 영향

배진규,김영수,김세은,심경미,강성수,조익현,이수한,박창현,엄창섭,정문진,한송이,임성철,배춘식,Bae, Jin-Gye,Kim, Young-Soo,Kim, Se-Eun,Shim, Kyung-Mi,Kang, Seong-Soo,Cho, Ik-Hyun,Lee, Soo-Han,Park, Chang-Hyun,Uhm, Chang-Sub,Jeong, Moon-Jin,Han 한국현미경학회 2006 Applied microscopy Vol.36 No.2

Gemifloxacin은 대부분의 G(-) 세균들과 G(+) 세균들에 대해 강한 활성을 나타내며 미성숙동물에서 비교적 낮은 연골독성 가능성을 가진 합성 fluoroquinolone계 항생제이다. 본 연구는 미성숙 SD계 랫드의 아킬레스건에 gemifloxacin이 미치는 영향을 평가하기 위해 gemifloxacin을 생후 4주 후부터 0(vehicle), 600mg/kg body weight 용량으로 5일 동안 매일 한번씩 경구투여를 하여 ofloxacin과 비교하였다. 아킬레스건의 형태학적 변화를 투과전자현미경을 이용하여 관찰한 결과 부형제만 처치한 대조군과 비교하였을 때, gemifloxacin과 ofloxacin을 처치한 랫드로부터 채취한 샘플에서 초미세구조의 변화가 관찰되었다. quinolone을 처치한 군의 tenocyte에서는 다양한 퇴행성 변화가 관찰되었고, 세포외기질에서 분리된 세포들이 존재하였는데 이는 ofloxacin을 처치한 그룹에서 특히 높았고 gemifloxacin을 처치한 그룹에서는 매우 낮았다. 따라서, gemifloxacin이 아킬레스건과 같은 결합조직 구조물들의 퇴행성 변화를 보다 적게 야기한다는 것을 보여준다. Gemifloxacin is a synthetic fluoroquinolone antimicrobial agent that exhibits potent activity against most Gram-negative and Gram-positive organisms, and has a comparatively low chondrotoxic potential in immature animals. This study examined the effects of gemifloxacin on the Achilles tendons in immature Sprague-Dawley rats treated by oral intubation once daily for 5 consecutive days from postnatal week 4 onward at doses of 0 (vehicle), and 600mg/kg body weight Ofloxacin was used for comparison. The Achilles tendon sperimens were examined by electron microscopy. In comparison with the vehicle-treated controls, there were ultrastructural changes in all samples from the gemifloxacin- and ofloxacin-treated rats. Degenerative changes were observed in the tenocytes, and the cells that detached from the extracellular matrix were recognizable. The degree of degenerative changes and the number of degenerated cells in the Achilles tendon were significantly higher in the treated group than in the control group. Moreover, among the quinolone treated groups, these findings were more significant in the ofloxacin treated group, and less significant in the gemifloxacin treated group. It is unclear what these findings mean with respect to the possible risk ill juvenile patients treated with gemifloxacin or other quinolones. However, these results show that gemifloxacin causes fewer changes in the connective tissue structures.

빵효모의 자기분해에 대한 몇가지 처리의 효과

최임순(Im-Soon Choi),심경미(Kyung-Mi Shim) 한국식품영양과학회 1984 한국식품영양과학회지 Vol.13 No.3

빵효모의 자기분해를 촉진시키기 위해서 50℃에서 12시간 동안 자기분해를 진행시키기 전에, 몇가지 화학적, 생화학적 및 물리적 처리를 행하여 다음과 같은 결과를 얻었다.<br/> 1. 0.01N-NaOH 용액의 묽은 알카리를 처리한 결과 자기분해 초기에는 비처리 대조구에 비해 현저하게 자기분해율이 증가하였으나 6시간이후에는 대조구와 별차이가 나타나지 않았다. 한편 그 이상의 알카리 농도에서는 오히려 추출율이 감소하는 경향을 보여주었다.<br/> 2. 효모의 자기분해물을 10% 첨가하여 자기분해를 진행시킨 결과 추출수율이 18% 이상 증가하였다. <br/> 3. 초단파 처리의 경우 40초 동안 처리하여 효모슬러리의 온도가 63℃ 일때는 15% 이상의 추출율증가가 나타났으며 그 이상 처리한 결과 고온으로 인해 추출수율이 감소하였다.<br/> 4. French Press에 의해 16,000~20,000psi의 고압으로 세포를 파괴한 결과 자기분해 9시간 까지는 추출액중 가용성 질소의 량이 75% 수준까지 높다가 그 이후에는 무처리구 보다 낮아지는 결과를 나타냈다. Some chemical, biochemical and physical treatments on the baker's yeasts (Saccharomyces cerevisiae) before 12-hour digestion at 50℃ were made to accelerate their autolysis.<br/> Dilute alkali treatment with 0.01N-NaOH solution showed a considerable increase in soluble nitrogen extraction only in the 6-hour initial autolysis. The addition of fresh yeast autolysate, 10% to the yeast slurry, could increase the autolysis rate from 65% to 83%. Microwave treatment of yeast slurry for 40 seconds also raised the autolysis rate by about 15%, while longer exposures to the microwave accompanying high temperatures repressed the autolysis process. Extrusion of the yeast cells with high pressures of 16,000 to 20,000 psi brought about significantly higher autolysis rate until the digestion time of 9 hours, but thereafter it showed a gradual drop in soluble nitrogen extraction.

비글견 경골에서 임플란트 식립 시 혈소판 농축 혈장이 골형성에 미치는 영향

국민석(Min-Suk Kook),정승곤(Seunggon Jung),심경미(Kyung-Mi Shim),강성수(Seong-Soo Kang),박홍주(Hong-Ju Park),유선열(Sun-Youl Ryu),오희균(Hee-Kyun Oh) 대한구강악안면외과학회 2010 대한구강악안면외과학회지 Vol.36 No.2

Introduction: The purpose of this study is to evaluate the effect of platelet rich plasma in bone formation and osseointegration of implant installed in the bone marrow space. Materials and Methods: Five beagle dogs were used as experimental animals. Two implants were installed on each tibia in the dogs. Implants of the control group were installed with no additional graft. Those of the experimental group 1 were installed with autogenous bone graft from the ilium autogenous bone. Platelet rich plasma (PRP) alone was used in experimental group 2 and the mixture of PRP and autogenous bone was used in experimental group 3. The animals were sacrificed at 8 weeks after implantation. The parts of tibia containing implant were harvested and radiographs were taken for radiographic examination. The specimens were prepared for histological examination and histomorphometric analysis of implant-bone contact ratios. Results: 1. All implants showed sufficient osseointegration in the cortical bone radiographically and histologically, but osseointergration in the marrow space was not satisfactory. 2. Histomorphometrically, the implant-bone contact ratios in the bone marrow was sequentially high in the experimental group 3 (autogenous bone+ PRP group), group 1 (autogenous bone group), group 2 (PRP group), and control group (non-additive). 3. It was verified that there was statistical significance between two experimental groups (group 1 and 3) and the other groups (group 2 and control group). (P<0.005) 4. However, there was no statistical significance between group 3 and group 1, also group 2 and control group respectively. Conclusion: These results suggest that platelet rich plasma is effective to osseointegration in the implant installation but there is no statistical significance.