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생쥐에 있어서 單一害球가 分離된 受精卵의 移植에 의한 새끼生産에 관한 硏究
崔有林,全益秀,金宣宜,崔光洙,朴修奉,辛明宰 慶北大學校農業科學技術硏究所 1991 慶北大農學誌 Vol.9 No.-
本 연구는 생쥐의 4細胞期胚에서 하나의 割球를 뽑아내는 새로운 割球分離技術인 biopsy에 대한 효율성과 分離된 受精卵과 分離한 割球의 생존성 및 새끼생산율을 검토한 結果이다. 그 結果를 要約하면 다음과 같다. 1. 4細胞期胚에서 分離한 單一割球와 4細胞期胚를 M2 배양액에 배양한 結果, 單一割球는 82.6%가 영양배엽을 형성한 割球로 發生하였고 4細胞期胚로 89.5%가 胚盤胞期胚로 發生하였다. 2. 4細胞期胚를 biopsy하여 하나의 割球가 分離된 受精卵과 대조구인 4細胞期胚를 M2배양액에서 배양한 결과 각각 83.3%와 90.4%가 胚盤細胞胚로 發生하였다. 3. Biopsy하여 4細胞期胚에서 分離한 單一割球와 대조구인 4細胞期胚에서 4개의 割球로 분리한 單一割球를 M2 배양액에서 배양한 결과 각각 80.8%와 83.3%가 영양배엽을 형성한 割球로 發生하였다. 4. 4細胞期胚에서 하나의 割球를 뽑아낸 受精卵과 대조구인 4細胞期胚를 수란생쥐에 이식한 결과 각각 36.0%와 48.6%의 새끼쥐 생산率을 얻었다. The study was carried out to investigate the viability and the offspring production rate of single blastomeres and biopsied 4-cell mouse embryos and, also to examine the efficiency of biopsy. The results obtained are summerised as follows : 1. The separated blastomeres from 4-cell embryos and intact 4-cell embryos which were cultured in Medium 2 were developed to trophoblastic vesicle and blastocyst embryo by 82.6% and 89.5%, respectively. 2. The biopsied embryos from 4-cell embryos and intact 4-cell embryos which cultured in Medium 2 were developed to blastocyst embryo by 83.3% and 90.4%, respectively. 3. The biospied blastomeres and the separated blastomeres from 4-cell embryos which were cultured in Medium 2 were developed to trophoblastic vesicle by 80.8% and 83.3%, respectively. 4. The biopsied embryos from 4-cell embryos and intact 4-cell embryos were transferred to recipients, and the offspring rate was 36% and 48.6%, respectively.
밀 유전자원의 농업형질과 SSR마커를 이용한 다양성 분석
신명재(Myoung-Jae Shin),오미애(Miae Oh),김경민(Kyung-Min Kim),왕샤오한(Xiaohan Wang),김성훈(Seong-Hoon Kim),한세희(Seahee Han),Kebede Taye Desta(Kebede Taye Desta ),최유미(Yu-Mi Choi),윤혜명(Hye-myeong Yoon),이윤정(Yoonjung Lee) 한국육종학회 2022 한국육종학회지 Vol.54 No.4
Breeders typically use core collections of a specific trait or core collections selected by a few genotypes to efficiently select breeding materials or to study functional genes. As a result, many accessions have been deemed redundant or duplicated and are no longer considered for use. This study aimed to investigate the agronomical characteristics and genetic diversity of 1,514 previously unused and unstudied wheat germplasms and to confirm their value as breeding materials using population structure analysis. The performance of these wheat germplasms was compared with that of 8,878 wheat breeding materials and 72 known Korean wheat cultivars. The results of agronomic trait diversity comparison showed that the germplasm populations used for breeding research did not completely encompass the unused germplasm populations. The agronomic traits of wheat germplasms varied greatly. ANOVA and PCA results revealed the greatest differences in growth habits (CV=0.339), panicle length (CV=0.330), and awn length (CV=0.296). To accurately assess the value of unused wheat germplasms as breeding materials, 106 SSR markers were extracted from the analysis of four representative Korean cultivars: Geuru, Geumgang, Uri, and Jokyoung. Among these, 24 SSR markers were chosen, and 129 wheat resources were subjected to population structure analysis, which revealed five subpopulations. Most of the 34 germplasms that originated in Korea were distributed in subpopulation 1 (18 accessions, 52.9%) and subpopulation 4 (12 accessions, 35.3%). Subgroups 2, 3, and 5 differed significantly in agronomic traits and genotypes, indicating their potential as breeding materials. The findings of this study could serve as a foundation for breeders and aid in the discovery and utilization of new wheat breeding materials.