가정폭력 여성피해자 자녀의 공격성 및 우울에 대한 동물매개치료의 효과

송윤오 ( Yun O Song ),김종수 ( Jong Shu Kim ),조재현 ( Jae Hyun Cho ),박배근 ( Bae Kun Park ),김충희 ( Chung Hui Kim ) 한국놀이치료학회 2011 한국놀이치료학회지(놀이치료연구) Vol.14 No.3

본 연구는 쉼터 거주 가정폭력 여성피해자 자녀의 공격성 및 우울에 대한 동물매개치료의 효과를 알아보고자 진행되었다. 경남 진주의 쉼터에 거주하고 있는 7~12세 아동들을 대상으로 하였다. 아동들에 대한 공격성은 Buss와 Perry(1992)가 개발한 척도를 박현선(1998)이 충동적 공격성과 관련한 5개 문항을 적용하였고, 우울은 Zung, Richards와 Short(1965)가 개발한 것을 김철환 등(2000)이 표준화한 20개 문항의 척도를 사용하였다. 쉼터 거주 아동들에 대한 동물매개치료를 수행한 결과 공격성과 우울 모두에서 유의성 있는 치료의 효과를 나타내었으며, 특히 공격성에서 다소 높은 효과를 나타내었다. 이는 아동들이 성인에 비하여 보다 쉽게 스트레스에 의한 공격성 성향을 가지게 되는 반면 치료의 효과도 비교적 빠르게 나타나는 것으로 생각된다. 동물매개치료가 아동들에게 효과를 나타내는 것은 아동들이 성인에 비하여 애완동물에 대한 관심과 호기심이 많기 때문에 나타나는 결과라고 보인다. 따라서 앞으로 가정폭력에 노출된 아동들에게 공격성 및 우울에 대한 동물매개치료를 실시 할 경우 보조적 치료를 통하여 정서적 함양의 효과가 나타날 것으로 기대된다. 이러한 긍정적인 행동변화를 보여준 점에서 연구의 의의가 있다. This study was designed to investigate the effects of animal-assisted therapy program on aggression and depression by comparing before and after each therapy for children of women victims of family violence at shelters. Data were collected from sixteen children of 7~12 years old showing aggression and depression from M family violence shelter in Jinju. A single therapy was performed weekly, up to 12 times. The criteria of aggression and depression consist of 5 and 20 questions, respectively. The aggression and depression were shown to reduce by 12% and 6.65%, respectively, in 8 children of the family violence women victims at shelters at the completion of the therapies. The animal-assisted therapy program exerts more pronounced effect on aggression than depression. The results suggest that the animal-assisted therapy program may serve as an effective method to ameliorate the aggression and depression of children of family violence women victims at shelters.

대나무 분말의 항산화력과 돼지의 면역 활성에 미치는 영향

송윤오 ( Yun O Song ),추교문 ( Gyo Moon Chu ),장선희 ( Sun Hee Jang ),구애진 ( Ae Jin Goo ),고응규 ( Yeoung Gyu Ko ),하지희 ( Ji Hee Ha ),이재형 ( Jae Young Lee ),강석남 ( Suk Nam Kang ),송영민 ( Young Min Song ),조재현 ( Jae Hyeo 한국동물위생학회 2014 韓國家畜衛生學會誌 Vol.37 No.2

본 연구는 대나무 분말의 항산화 활성이 비육돈에서 면역조절 효과에 미치는 영향을 구명하기 위해 실시하였으며, 이를 위해 대나무 분말 추출물의 항산화능을 검정하기 위하여, 총페놀 함량, 플라보노이드함량 및 DHHP, ABST, hydroxyl radical 등의 소거활성능을 알아보았다. 대나무 추출물의 항산화능은 높게 나타났으며, DPPH와 ABTS, HRSA 라디칼 소거능은 12∼21%였으며, 추출물의 유효성분으로 총페놀 함량은 171 mg/g이었으며, 플라보노이드 함량은 127.mg/g으로 나타났다. 다음으로 대나무 분말이 함유된 배합사료 급여시 돼지의 면역력에 미치는 영향을 알아보았다. 처리구에는 일반사료를 급여하였고, 시험구는 일반사료에 대나무분말을 1.0%, 2.0% 및 3.0%을 첨가하여 급여하였다. 대나무분말 첨가수준에 따라 혈액의 혈구계수 중 백혈구, 적혈구, 헤마토크리트 함량에서 유의적인 수준의 차이를 나타내지 않았다. 하지만 헤모글로빈 함량이 무처리 대조구에 비교하여 증가하였으며 특히 2∼3%의 처리구에서 헤모글로빈 함량은 높게 나타났다. 혈액 내 총단백질, 혈중알부민의 농도를 분석한 결과 처리구와 무처리구간에 유의적인 차이를 나타내지 않았다. AST, ALT 함량은 대나무 분말 처리군에서 무처리군보다 약간 높게 나타나지만 정상적인 범위내에서의 변화를 보였다. immunoglobulin에서 IgA와 IgG 농도는 무처리구에 비교하여 대나무 분말 처리구에서 높게 나타나고, IgA는 처리구 간의 차이가 나타나지 않았지만 IgG 농도에서는 대나무 추출물 2%, 3% 처리구가 1% 처리구에 비해 높은 수준을 나타냈다. TNF-α의 경우 무첨가구와 첨가구 사이의 유의적인 차이가 없었지만, 대나무 분말 첨가구에서 interferon-γ의 함량은 모든 첨가구에서 무처리 군보다 유의적으로 높게 나타났다. 특히 2%, 3% 처리구가 1%의 처리구 보다도 높은 interferon-γ의 함량을 나타내었다. 이는 대나무의 페놀, 또는 플라보노이드 성분에 기초한 높은 항산화력에 의하거나, 또는 대나무에 포함된 돼지 초기 면역반응을 활성화시킬 수 있는 물질에 의한 면역활성 증강에 따른 것으로 사료된다. 결론적으로 대나무 분말의 높은 항산화력은 돼지 면역력 향상에 기여하였으며, 특히 2∼3% 대나무 분말 첨가 사료는 돼지 생리활성을 크게 증강시키는 것으로 사료된다. The present study was designed to explore the antioxidant effect of Bamboo powder and its immunoreactivity in pigs. We investigated the functional properties of Bamboo extracts by means of measuring the contents of total polyphenols and flavonoid as well as determining ABST, DPPH radical scavenging activity, and hydroxyl radical scavenging activity and anticancer activity. The total phenolic compound and flavonoids contents of Bamboo extracts were 171.25 mg/g and 127.5 mg/g, respectively. The DPPH radical, hydroxyl radical, ABST radical scavenging activity of Bamboo extracts were 17.3%, 12.5% and 21.5%, respectively. Evidenced by MTT and cell cycle assay, Bamboo dose-dependently inhibited the cell proliferation and induced G0/G1-phase arrest in CHO cells at concentrations of 100, 250, and 500 μg/ml Bamboo extracts. More than 80% of apoptotic cells were observed by staining with annexin V in 500 μg/ml Bamboo-treated CHO cells, indicating that Bamboo had potent anticancer activities. Next, to investigate the effect of Bamboo on cytokine, immunoglobulin concentration, and blood compositions, flatting pigs were fed with Bamboo powder for 38 days. Flatting pigs were divided into 4 groups; basal diet (control), basal diet supplemented with 1% Bamboo powder (T1), 2% Bamboo powder (T2), and 3% Bamboo powder (T3). The level of hemoglobin increased in the all Bamboo-fed groups compared with the normal control group. In particular, platelet levels in the all Bamboo-treated groups increased by approximately 90% compared with the levels from pig on a normal control. Serum levels of immunoglobulins (IgG, IgA) in the pigs fed Bamboo powder were modestly increased, and the interferon-γ level also was strongly increased in 2% or 3% Bamboo-fed groups compared with the levels in control groups. Together, these results demonstrated that Bamboo extracts had an effective capacity of scavenging for ABTS, DPPH, and hydroxyl radicals and showed correlation with potent phenol and flavonoid contents, thus suggesting its antioxidant potential. Moreover, administration of Bamboo in 2∼3% improved blood parameters and platelets, and especially immunity-related ones such as IgG, IgA, and interferon- γ, leading to be potential feed additives in flatting pigs.

고지방 식이에 의한 흰쥐의 지방간증에서 오미재 추출물의 간보호 효과

송윤오 ( Yun O Song ),이수정 ( Soo Jung Lee ),박현준 ( Hyoung Joon Park ),장선희 ( Sun Hee Jang ),정병엽 ( Byung Yeoup Chung ),송영민 ( Young Min Song ),김곤섭 ( Gon Sup Kim ),조재현 ( Jae Hyeon Cho ) 한국동물위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.1

The purpose of the present study is to determine whether Schisandra chinensis (SC) has a protective effect on high fat diet (HFD)-induced fatty liver including hepatic lipid accumulation in rats. The HFD-induced obese rats were weighed after SC extracts were administered through the gastrointestinal tract at a concentration of 250 mg/kg b.w/day for 5 weeks. After 5 weeks, all of the rats on a high fat-diet were 36.5% heavier compared with normal controls. In contrast, rats on a high-fat diet supplemented with SC were 23.5% lighter than rats fed only a high-fat diet. Although there was no significant difference in food intake among the groups during the experimental diet period, the body weight gain of the SC group was significantly lower than the weights of the HFD groups. SC treatment slightly decreased the liver weight. Reduction of hepatic TBARS contents by SC was observed in rats fed a diet containing SC, and antioxidant activity was markedly increased in HFD+SC group compared to those of HFD group in liver. Moreover, total-lipid and triglyceride contents in the liver of groups fed a diet containing SC were significantly lower compared to those of the HFD group. High fat feeding elevated liver cholesterol concentration, but the addition of SC to the HFD rats resulted in the significant decrease in liver cholesterol. In histological observation of liver tissues, the hepatocytes of HFD rats showed a typical fatty liver morphology showing numerous lipid droplets in cytoplasm, whereas administration of SC reduced the size and numbers of lipid droplets. These results clearly demonstrated the attenuation of SC on nonalcoholic fatty liver induced by obese rats fed HFD.

벌나무(Acer tegmentosum Maxim) 추출물의 항산화 활성 및 고지방식이를 급이한 흰쥐의 지방간 개선 효과

이수정,조항희,송윤오,장선희,조재현 한국동물위생학회 2019 韓國家畜衛生學會誌 Vol.42 No.2

The effects on the radical scavenging activities and hepatic lipid levels in rats fed a high-fat diet (HFD) in the 70% ethanolic extract from Acer tegmentosum Maxim (ATM) were evaluated. Total phenol con-tent of ATM was 168.60 mg catechin/g in the 70% ethanolic extract of Acer tegmentosum. The DPPH and ABTS radical scavenging activities were 18.32 mM TE/g and 32.25 mM TE/g, respectively. Food efficiency ratio was lower significantly in supplemented group with 150 mg/kg BW/day during 5 weeks (HFD+ATM) compared to HFD. Total cholesterol and triglyceride contents in liver tissue of HFD+ATM were lower significantly compared to those of the HFD. Supplementation of ATM significantly de-creased lipid peroxide contents and increased radical scavenging activity in the liver tissue compared with that of HFD group. Moreover, the hepatocytes of HFD rats showed a typical fatty liver morphol-ogy showing the presence of cytoplasmic lipid droplets, whereas administration of ATM attenuated the number and the size of lipid droplets. In the liver tissue of ATM administrated HFD group, the mRNA levels of SREBP-1c, PPAR, and FAS were decreased. Therefore, these results suggest that Acer teg-mentosum extracts could have antioxidant activities and the hypolipidemic effects in liver tissue by its phenolic compounds.

근전도 신호와 BLDC모터 제어를 통한 무릎재활시스템

권형기,고형규,송윤오,손의성,이붕주 한국전자통신학회 2019 한국전자통신학회 논문지 Vol.14 No.5

This research is the design and implementation of a rehabilitation medical device based on a EMG measurement. Rehabilitation systems are controlled using BLDC motors and motor drives. The BLDC motor drive controls the operation and the speed controls the drive through the external servo motor. The potentionmeter coupled to the outside of the motor transmits information about the position of the load being rotated by the motor. The rehabilitation algorithm executes the periodic contraction relief rehabilitation at a certain angle range by speed stage and controls the maximum angle of 0-120 using the motor according to the user setup stage during the rehabilitation exercise. The walking algorithm compensates motor control for legs with low avionics signal by motor control using differential values of signals obtained by EMG measurement attached to both innermost roots and controls the maximum angle of 0 to 88 using motor and EMG measurement during walking motion. In addition, the application implements a more convenient knee rehabilitation system to confirm the feasibility of the application. 본 연구에서는 표면근전도 측정기 기반의 재활 의료기기의 설계 및 구현에 관한 내용을 기술한다. 재활시스템은 BLDC모터와 모터 드라이브를 이용하여 제어된다. BLDC모터 드라이브는 동작 제어를 하며, 속도는 외부 서보모터를 통해 드라이브를 제어한다. 모터 외부에 결합된 포텐쇼미터는 모터에 의해 회전하는 부하 위치 정보를 전달한다. 재활 알고리즘은 환자가 일정 각도 범위에서 주기적인 수축⋅이완 재활운동을 속도단계별로 실행하며 재활운동 시 사용자 설정 단계에 따른 모터를 활용하여 0∼120[]의 최대각도를 제어한다. 보행 알고리즘은 양쪽 안쪽넓은근에 부착된 표면근전도 측정기로 획득한 신호의 차이 값을 이용하여 근전도 신호가 낮은 다리에 모터제어로 보상해주며 보행운동 시 모터와 표면근전도 측정기를 활용하여 0~88[]의 최대각도를 제어한다. 또한, 어플리케이션으로 보다 편리한 무릎재활시스템을 구현하여 실용화 가능성을 확인하였다.

Schisandra chinensis Prevents Alcohol-Induced Fatty Liver Disease in Rats

박형준,이수정,송윤오,장선희,고응규,강석남,정병엽,김홍덕,김곤섭,조재현 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.1

Schisandra chinensis (SC), a traditional herbal medicine, has been prescribed for patients suffering from various liver diseases, including hepatic cancer, hypercholesterolemia, and CCl4-induced liver injury. We investigated whether SC extract has a protective effect on alcohol-induced fatty liver and studied its underlying mechanisms. Rats were fed with ethanol by intragastric administration every day for 5 weeks to induce alcoholic fatty liver. Ethanol treatment resulted in a significant increase in alanine aminotransferase, aspartate aminotransferase, and hepatic triglyceride (TG) levels and caused fatty degeneration of liver. Ethanol administration also elevated serum TG and total cholesterol (TC) and decreased highdensity lipoprotein (HDL) cholesterol levels. However, after administration of ethanol plus SC extracts, the ethanol-induced elevation in liver TC and TG levels was reversed. Elevation in serum TG was not observed after treatment with SC. Moreover, compared with the ethanol-fed group, the rats administered ethanol along with SC extracts for 5 weeks showed attenuated fatty degeneration and an altered lipid profile with decreased serum TC and TG, and increased HDL cholesterol levels. Chronic ethanol consumption did not affect peroxisome proliferator-activated receptor c (PPARc) levels, but it decreased PPARa and phospho-AMP-activated protein kinase (AMPK) levels in the liver. However, SC prevented the ethanol-induced decrease in PPARa expression and induced a significant decrease in sterol regulatory element-binding protein-1 expression and increase in phospho-AMPK expression in rats with alcoholic fatty liver. SC administration resulted in a significant decrease in intracellular lipid accumulation in hepatocytes along with a decrease in serum TG levels, and it reversed fatty liver to normal conditions, as measured by biochemical and histological analyses. Our results indicate that the protective effect of SC is accompanied by a significant increase in phospho-AMPK and PPARa expression in hepatic tissue of alcoholic rats, thereby suggesting that SC has the ability to prevent ethanol-induced fatty liver, possibly through activation of AMPK and PPARa signaling.

적외선 체열촬영술을 이용한 thoroughbred 말의 근골격계 파행진단 증례

박형준 ( Hyoung Joon Park ),송윤오 ( Yuno Song ),이진주 ( Jin Ju Lee ),지창무 ( Chang Moo Ji ),정해원 ( Hae Won Jung ),김석 ( Suk Kim ),조규완 ( Kyu Won Cho ),조재현 ( Jae Hyeon Cho ),김종수 ( Jong Shu Kim ),문운경 ( Oun Kyong Mo 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.3

The mare had lameness and asymmetry edematous on its tarsal and metatarsal joints at the initial physical examination. The pain was elicited with a palpation along the metatarsal articulations. No significant abnormalities were detected in the screening test. However, thermographic images revealed a significant increase in the surface temperature at the joint of the hindlimb when compared to the reference range. At necropsy, an irregularity of the surface and excessive synovial fluid were observed on the right tarsal joint. No bacterial growth was shown in the cultures of synovial fluid. Staphylococcus aureus was detected in the subcutaneous discharge. Taken together, the thermography images were very useful in localizing the area of injury and were an effective diagnostic methodology for assessing lameness.

평면도형 높이에 대한 학생 이해도와 오류 유형

이광호,이현주,이주영,송윤오 한국교원대학교 뇌기반교육연구소 2014 Brain & Learning Vol.4 No.2

The purpose of the research is to firstly, understand 5th graders conceptions of heights in triangles and parallelograms. Secondly, analyze and categorize the frequently shown error types of the concept of heights in triangles and parallelograms. Thirdly, think about the cause and counterplan of the students’ misconceptions of heights from the perspective of concept definition and concept image of Vinner model. 433 (19 classes) 5th graders of 6 elementary schools in Chungcheong area of South Korea were tested to draw the heights on a questionnaire containing 14 triangles and 12 parallelograms with varied positions and shapes. From the study, we could conclude as followings. There is a need to include wordings such as ‘the side opposite the vertex’ and ‘a line containing the base(extended base)’ within the definition of heights. The meaning of the words used in the definition and used in everyday life should be clearly discriminated. When teaching the concept of heights in plane figures, teachers should utilize various example and counter-example images based on the errors students frequently make.

먹물버섯(Coprinus comatus )의 항산화 활성 및 Zucker rat에 대한 항비만 효과

이수정 ( Soo Jung Lee ),박형준 ( Hyoung Joon Park ),송윤오 ( Yuno Song ),장선희 ( Sun Hee Jang ),구애진 ( Ae Jin Goo ),고응규 ( Yeoung Gyu Ko ),조재현 ( Jae Hyeon Cho ) 한국동물위생학회 2014 韓國家畜衛生學會誌 Vol.37 No.1

The aim of the study was to investigate the anti-obesity effect of Coprinus comatus (CC) in high-fat diet-fed Zucker rat (fa/fa). Obesity was induced by feeding on high-fat diet (HFD) containing 60% kcal fat for 10 weeks, in which CC extracts were administrated through the gastrointestinal tract at a concentration of 200 mg/kg BW/day for 10 weeks. The total polyphenol and flavonoid contents of CC extracts were found to be 18.5±1.1 mg of catechin equivalent/g, and 5.24±0.54 mg of quercetin equivalent/g extract, respectively. The DPPH, ABTS, and hydroxyl radical scavenging activities of CC extracts were 15.34 %, 17.25%, and 16.18%, respectively. In animal study, CC administration significantly reduced the body weight, while there were no significant differences in the daily food intake between the HFD-fed Zucker rats and HFD plus CC-fed rats. CC treatment decreased epididymal and perirenal fat weights in HFD-fed Zucker rats. Significant decreases in the levels of triglyceride and total cholesterol in the serum and liver were observed in the CC-treated group compared with HFD-fed Zucker rats.Serum HDL-cholesterol levels in the CC-treated group were increased compared with the HFD-fed groups. Serum AST and ALT activities in the CC group were significantly lower than those of the HFD-fed group. Taken together, these data demonstrated that CC has potential in preventing high-fat diet induced obesity and is a good candidate for an anti-obesity agent.

근전도 신호 분석 및 BLDC모터 제어를 통한 무릎재활시스템

권형기(Hyeong-Gi Kwon),고형규(Hyeong-Gyu Ko),송윤오(Yoon-Oh Song),손의성(Eui-Seong Son),이붕주(Boong-Joo Lee) 한국전자통신학회 2019 한국전자통신학회 논문지 Vol.14 No.5

본 연구에서는 표면근전도 측정기 기반의 재활의료기기의 설계 및 구현에 관한 내용을 기술한다. 재활시스템은 BLDC모터와 모터 드라이브를 이용하여 제어된다. BLDC모터 드라이브는 동작제어를 하며, 속도는 외부서보모터를 통해 드라이브를 제어한다. 또한 모터 외부에 결합된 포텐쇼미터는 모터에 의해 회전하는 부하 위치 정보를 전달한다. 재활 알고리즘은 환자가 일정 각도 범위에서 주기적인 수축⋅이완 재활운동을 속도단계별로 실행하며 재활운동 시 사용자 설정 단계에 따른 모터를 활용하여 0~120[0]의 최대각도로 제한하여 제어한다. 보행 알고리즘은 양쪽 안쪽넓은근에 부착된 표면근전도 측정기로 획득한 신호의 차이 값을 이용하여 근전도 신호가 낮은 다리에 모터제어로 보상해주며 보행운동 시 모터와 표면근전도 측정기를 활용하여 0~88[0]의 최대각도로 제한하여 제어한다. This paper describes the design and implementation of a rehabilitation medical device based on a EMG measurement. Rehabilitation systems are controlled using BLDC motors and motor drives. The BLDC motor drive controls the operation and the speed controls the drive through the external servo motor. In addition, potentiometer coupled to the outside of the motor transmits information about the position of the load being rotated by the motor. The rehabilitation algorithm is controlled by limiting the maximum angle of 0 to 120 by utilizing the motor according to the user setting stage during the rehabilitation exercise. The walking algorithm compensates motor control for the low leg of the signal using the difference value of the signal obtained with the surface denser attached to both inner muscles. The motor and surface denser are utilized for the walk motion to control the maximum angle of 0 to 80.