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호장근으로부터 분리된 emodin의 혈관신생 억제 활성
이태규,김종화,소준노,Lee, Tae-Kyoo,Kim, Jong-Hwa,So, June-No 한국응용생명화학회 2003 한국농화학회지 Vol.46 No.1
호장근에서 분리한 emodin은 VEGF로 유도된 혈관신생의 한 단계인 혈관내피세포의 이동을 강하게 억제하였다. 또한 emodin은 혈관내피세포 이동을 억제시킨 $0.1\;{\mu}g/ml$ 농도에서 시험관내 혈관신생을 억제하였으며, 그 효과는 농도의존적인 양상을 보였다. 생체내 혈관신생 모델인 CAM assay에서도 emodin은 혈관신생을 억제하였다. 이러한 결과는 emodin이 현재까지 보고된 여러 가지 생리활성 이외에 혈관신생 억제활성을 가지고 있다는 사실을 보여주는 것이다. 따라서 emodin을 함유하고 있는 호장근은 혈관신생 관련 질환에 대한 천연물 유래 치료제의 개발을 위한 중요한 식물자원으로 활용될 수 있을 것이다. Polygonum cuspidatum has been used as a fork medicine for a long time. Emodin was purified from the root of P. cuspidatum by thin layer chromatography (TLC) and preparative high perfomance liquid chromatography (HPLC). The effects of emodin on the migration of endothelial cells and in vitro angiogenesis stimulated with vascular endothelial cell growth factor (VEGF) were examined, using human umbilical vein endothelial cells (HUVECs) and porcine pulmonary arterial endothelial cells (PPAECs). Emodin potently inhibited the VEGF-induced migration of (HUVECs) at relatively low cocentrations $(0.1-10\;{\mu}g/ml)$; the inhibition of endothelial cells by emodin was 75.4% at $0.1\;{\mu}g/ml$ and about 90% at $1\;{\mu}g/ml$. Emodin also inhibited VEGF-induced sprout formation in vitro at concentrations of $0.1-10\;{\mu}g/ml$. Emodin was also evaluated for the inhibitory potential on in vivo angiogenesis in a growing chick embryo chorioallantoic membranes (CAM). At a concentration of $1.0\;{\mu}g/ml$ Per disc, emodin was able to induce avacular zone in the CAMs. These findings suggest that emodin is a potent angiogenesis inhibitor and P. cuspidatum is a useful herb in the development of therapeutics for angiogenesis dependent diseases.
신성 고혈압 백서에서 Atrial Natriuretic Peptide의 신장기능에 미치는 효과
조경우,김선희,소준노,류훈,설경환,Cho, Kyung-Woo,Kim, Suhn-Hee,So, June-No,Ryu, Hoon,Seul, Kyung-Hwan 대한생리학회 1989 대한생리학회지 Vol.23 No.1
Since the atrial receptor was suggested to be involved in the control of extracellular fluid volume, it has been shown that the granularity of atrial cardiocytes can be changed by water and salt depletion, and that an extract of atrial tissue, when injected intravenously into anesthetized rats, causes a large and rapid increase in renal excretions of sodium and water. The immunoreactive atrial natriuretic peptide (ANP) has been found in the plasma of patients suffering from various cardiovascular diseases. A high level of ANP in the plasma has been reported in essential hypertension. Several studies on the effects of ANP on renal function and arterial blood pressure have presented contradictory results showing attenuated or accentuated responses. Thus, involvement of the ANP in the development of hypertension remains unresolved. Present study was undertaken to investigate whether the ANP is involved in the development of hypertension in two-kidney one-clip Goldblatt hypertensive rats. The plasma concentration of immunoreactive ANP appeared to be significantly elevated in hypertensive rats as compared with normotensive Goldblatt operated and sham-operated rats. Plasma renin concentration was higher in hypertensive rats than in normotensive rats, as observed in earlier experiments. Intravenous infusions of ANP resulted in increases of urine flow and urinary excretions of sodium and potassium in both hypertensive and normotensive rats. The renal response to ANP was markedly accentuated in Goldblatt hypertensive rats. The plasma concentration of ANP showed a linear relationship with the arterial blood pressure. Infusions of ANP reduced blood pressure both in hypertensive and normotensive rats. These results suggest that in Goldblatt hypertensive rats an elevation of ANP level in the plasma may not be a cause, but instead a consequence of hypertension, and that the renal responsiveness to the ANP is accentuated by some unknown mechanisms.
3T3-L1 세포(細胞)의 증식(增殖) 및 분화(分化)에 미치는 백복령(白茯笭), 택사(澤瀉) 및 창출(蒼朮)의 영향(影響)
은재순,홍종성,소준노,Eun, Jae-Soon,Hong, Jong-Sung,So, June-No 한국생약학회 1993 생약학회지 Vol.24 No.2
These studies were conducted to investigate the effects of the extracts from Hoelen alba, Alismatis Rhizoma and Atractylodes Rhizoma on the proliferation and differentiation of 3T3-L1 cells. The results were summerized as follows: Hoelen alba and Alismatis Rhizoma extracts inhibited the proliferation of preadipose 3T3-L1 cells. In inductive differentiation, all three extracts inhibited the adipose conversion in 2 days of initial-culture, Atractylodes Rhizoma extract inhibited the adipose conversion in 5 days of final-culture and Hoelen alba and Alismatis Rhizoma inhibited adipose conversion in treatment of whole term of culture. In spontaneous differentiation, Atractylodes Rhizoma extract increased the adipose conversion in 2 days of initial-culture, Hoelen alba and Alismatis Rhizoma increased the adipose conversion in 5 days of final-culture, all three extracts increased adipose conversion in treatment of whole term of culture. The 10% serum of mice treated with each sample did not affect, but the 5% serum of them inhibited the proliferation of 3T3-L1 cells. In inductive differentiation, the 10% serum of them inhibited the adipose conversion in treatment of whole term of culture.
이금영,장선일,오영안,이송재,소준노 ( Geum Yeong Lee,Seon Il Jang,Young Nam Oh,Song Jae Lee,June No So ) 한국하천호수학회 1987 생태와 환경 Vol.20 No.3
Chromosome banding patterns obtained by silver staining (Ag-NORs) were analyzed in two Misgurnus species. In M. mizolepis the Ag-NORs were found at the secondary constrictions of a single chromosome pair or on the short arms of acrocentric chromosome pair, while those of M. anguillicaudatus were found on the short arm of one large subtelocentric pair or two acrocentric chromosome pair. In two Misgurnus species, there seems to be a trend toward chromosomal dispersion rather than fusion of the Ag-NORs. The chromosome polyploidy were not observed.
섬유아세포(纖維芽細胞)(Balb/c 3T3)의 증식(增殖)에 미치는 신효탁리산(神效托裡散)의 영향(影響)
은재순,전용근,염정열,서은실,소준노,오찬호,Eun, Jae-Soon,Jeon, Young-Keun,Yum, Jeong -Yul,Suh, Eun-Sil,So, June-No,Oh, Chan- Ho 한국생약학회 1993 생약학회지 Vol.24 No.2
The studies were conducted to investigate the effect of Sinhyo-Taklee-San(STS), which is composed of Astragali Radix(AR), Lonicerae Flos(LF), Angelicae gigantis Radix(AGR) and Glycyrrhizae Radix(GR), on the proliferation of fibroblast cell(Balb/c 3T3). STS, GR and glycyrrhizin increased the proliferation of 3T3 cells. The 10% serum obtained from STS, AR, LF, AGR and GR treated mice also increased the proliferation of 3T3 cells markedly. GR, glycyrrhizin and glycyrrhetinic acid inhibited protein synthesis, but did not affect on DNA synthesis.
한국 (韓國) 및 일본산 (日本産) 버들치속 ( 잉어과 ) 어류 4종의 핵형분석 (核型分析)
이금영,장선일,윤명자,소준노,전상린,강영희,판정융언 (板井隆彦),군총방휘 (君塚芳輝) ( Geum Yeong Lee,Seon Il Jang,Myeong Ja Yun,June No So,Sang Rin Jeon,Young Hee Kang,Takahiko Itai,Yoshiteru Kimizuka ) 한국하천호수학회 1987 생태와 환경 Vol.20 No.1
Somatic chromosomes in 12 population of the four Moroco species of Cyprinidae from Korea and Japan were examined. The preparation were made to flame drying method, and the karyotypes were analyzed by conventional Giemsa-staining. Diploid chromosome number in 12 populations of the four Moroco species of Cyprinidae from Korea and Japan were all samed, 2n=50. The ranges were 88-92 in fundamental number (NF). Some chromosomal characteristics were detected which permit establishing some karyotypic relationshipes among the different population of each Moroco species investigated. Thus, among the Moroco, variable difference of the karyotypes in the four species observed between 12 populations indistinguishable in the morphological characters (M. steindachneri…in Tama River system Hirai River and Sagami River system Shinobara River: 10 metacentrics, 11 submetacentrics, 4 acro-telocentrics; Ara River system Nakuri River and Ihara River: 8 m, 13 sm, 4 a-t; Hatauchi River: 8 m, 12 sm, 5 a-t. M. jouyi … in Shigenobu River system Ishide River: 9 m, 12 sm, 4 a-t; Hatauchi River: 6 m, 15 sm, 4 a-t; Ihara River: 6 m, 13 sm, 6 a-t. M. lagowskii…in Sach`o^˘n-river: 6 m, 14 sm, 5 a-t. M. oxycephalus … in Imjin-river: 6 m, 14 sm, 5 a-t; Kokgang-river and Taejong-river: 8 m, 13 sm, 4 a-t.).
항암제(抗癌劑) Mitomycin C와 수종(數種) 복합생약(複合生藥)의 병용투여(倂用投與) 효과(I) -보익제(補益劑)-
안문생,김세길,은재순,임종필,염정열,서은실,오찬호,소준노,Ahn, Moon-Saeng,Kim, Sae-Gil,Eun, Jae-Soon,Lim, Jong-Pil,Yum, Jung-Yul,Suh, Eun-Shil,Oh, Chan-Ho,So, June-No 한국생약학회 1992 생약학회지 Vol.23 No.3
The studies were conducted to investigate the combined effects of several combined preparation of crude drugs and mitomycin C(MMC). The combined effects on the proliferation of Molt-4 cells and activation of human lymphocytes were estimated by MTT colorimetric assays. The drugs itself enhanced the proliferation of Molt-4, but the inhibitory action of MMC was not affected by the combined treatment of the drugs and MMC. Among 9 kinds of the drugs, Sip Jeon Dae Bo Tang(SDT), Saeng Maek San(SMS) and Kwi Bi Tang(KBT) did not inhibit the action of MMC, but activated lymphocytes. When the mice were treated by MMC, the number of leukocytes was decreased significantly at the 1st day, but recovered at the 7th day. In the groups of MMC treated with SDT or KBT, the number of leukocytes was increased significantly than the group of MMC treated only at the 3rd day. The combined treatment of SDT, SMS and MMC retained the body weight of mice at the level of normal mice. The SDT, SMS and KBT did not change the number of plaque forming cells(PFC) and the proliferation of T cells. The combined treatment of SDT and MMC increased the number of PFC significantly than the MMC treated group. The combined treatment of SDT, SMS, KBT and MMC increased the T cell proliferation significantly than the MMC treated group. In conclusion, it is suggested that SDT, SMS and KBT can recover the side effects of MMC, such as weight loss, leukopenia and immunosuppression, without any intercalating the anti-proliferative action of MMC in vivo.
박웅(Woong Park),유두만(Du Man Yoo),소준노(June No So) 한국생물공학회 2014 KSBB Journal Vol.29 No.1
Planaria were recently reported to be a simple and sensitive model to investigate the mechanistic aspect of seizure and to screen potential anticonvulsants. Using planarian model, we analyzed the pharmacological effect of ukgansan (UGS), an oriental herbal medicine containing seven medicinal herbs, on the planarian locomotor velocity (pLMV) and glutamate-induced seizure-like activity (pSLA). To test whether D. japonica is suitable for studying anti-seizure agents, we investigated the effect of glutamate on pLMV and pSLA in D. japonica. In the present study we first confirmed that pSLA in D. japonica was induced by L-glutamate. Glutamate significantly produced pSLA in a dose dependent manner, but did not affect pLMV. These glutamate-induced paroxysms were decreased by antiepileptic drug, topiramate. A similar inhibitory effect on glutamate-induced pSLA was observed after the treatment of UGS. The present results suggest that UGS and its active constituents possess useful substance inhibiting seizure in planarian and that D. japonica provides a convenient model to search active herbs containing anti-seizure activity.