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스트렙토조토신 유도성 당뇨쥐의 췌장 선포세포에서 Cholecystokinin이 유도한 Calcium Signal의 변화
김명준 ( Kim Myeong Jun ),류경렬 ( Lyu Gyeong Lyeol ),성종호 ( Seong Jong Ho ),민도식 ( Min Do Sig ),이덕주 ( Lee Deog Ju ),윤신희 ( Yun Sin Hui ),한상준 ( Han Sang Jun ),조양혁 ( Jo Yang Hyeog ),김명석 ( Kim Myeong Seog ) 대한소화기학회 2003 대한소화기학회지 Vol.42 No.6
Background/Aims: Pancreatic acini of streptozotocin (STZ)-induced diabetic rats release amylase less than normal acini on cholecystokinin (CCK) stimulation. Pancreatic enzyme secretion has been closely related to the intracellular calcium concentration ([Ca2+]i) of the acinar cell. In the present study, sequential changes of the intracellular calcium signal which probably underlie the altered enzyme secretion in response to CCK-8 were investigated using pancreatic acini from diabetic rats. Methods: Diabetic rats were prepared by single intravenous injection of STZ (70 mg/kg). Stimulating experiments with CCK-8 were performed 7 days later. Pancreatic acini were isolated by collagenase digestion. Amylase release and [Ca2+]i were measured by colorimethod and calcium imaging, respectively. The geometry of intracellular calcium signal was analyzed. Results: Normal acini exhibited concentration-dependent [Ca2+]i increase and regular oscillatory calcium signal on CCK-8 stimulation. Amylase release was also concentration-dependent. However, diabetic acini showed significantly less [Ca2+]i increase, prolonged time to peak [Ca2+]i, decreased calcium spikes number, and decreased amylase release compared with normal acini. The decreased [Ca2+]i in diabetic acini was restored significantly by insulin treatment. Conclusions: Relatively decreased amylase release in diabetic pancreatic acini in response to CCK, appears to be associated with altered calcium signal due to insulin deficiency. (Korean J Gastroenterol 2003;42:519-526)
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위점막에서 Helicobacter pylori 분포의 내시경적 진단
주영은(Young Eun Joo),김신묵(Sin Mook Kim),김태두(Tae Du Kim),신동현(Dong Hyun Sin),성종호(Jong Ho Seong),안영주(Young Joo Ahn),정용환(Yong Hwan Jeong),양대현(Dae Hyun Yang),유종선(Chong Sun Rew),윤종만(Chong Mann Yoon) 대한내과학회 1994 대한내과학회지 Vol.46 No.1
N/A Background: Since the successful isolation of Helicobacter pylori from the human gastric mucosa by Warren and Mashall, biosy studies have shown that this organism is closely related to chronic gastritis and peptic ulcer. However the distribution of this organism has been proved to be uneven and patchy on the gastric mucosa. The aim of this paper is to assess the distribution of this organism on human gastric mucosa in vivo by endoscopy. Method: To control the gastric pH around 4.0 to 6.0, premedication by the intravenous injection of ranitidine 50mg one hour before the endoscopy, was performed. And thereafter, 0.1% phenol red solution containing 0.5M urea was sprayed onto the mucosal surface of the stomach through the endoscope. A red color change was observed in a few seconds after spraying the phenol red solution. The red color change pattern was classified into three types: diffuse, regional and patchy type. Two biopsies were taken from the red color changed area and the unstained area for hitological and bacteriological study. Results: l) In 26 out of 33patients with chronic gastritis, the red color change was observed after spraying the phenol red solution. The diffuse, regional and patchy type in chronic gastritis patients were observed in 17, 5, 4 patients respectively. In peptic ulcer group, the diffuse type was observed in 6 and the regional and patchy type were observed in 3, 1 patients. 2) The bacteriological and histological identification of Helicobacter pylori was observed in 17 of 26patients (65.4%) With Chronic gastritis and 7 Of 10patiCIltS (70%) with peptic ulcer in the biopsy materials taken from the gastric mucosa showing a red color change with phenol red solution. But the organism was not found in the unchanged and unstained mucosa except for one case of chronic gastritis. Conclusion: These results suggest that this endoscopic spraying technique may be useful to determining the distribution of Helicobacter pylori in human gastric mucosa.
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김영진,윤경환,정용환,김세종,유종선,윤종만,신동현,안영주,성종호 대한소화기내시경학회 1992 Clinical Endoscopy Vol.12 No.2
The incidence of synchronous gastric cancer is variously reported to be 2.07%, 5.22%, 6.5%, 8.6%, or 9% and has been increasing recently, probably with advance in the diagnosis of gastric cancer. Our case in presentation is a 70-year-old male patient who had total gastrectomy and esopbagiojunostomy done for synchronous four gastric cancer. Each has different histologic types, Some of these lesions are not suspected before operation and are found almost by chance during histologic examinations after surgery performed only to remove main lesion. So we report this case with a review of literatures.
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김영진,범희승,조규혁,정용환,정상우,김세종,유종선,신동현,윤종만,양대현,성종호,안영주 대한소화기내시경학회 1993 Clinical Endoscopy Vol.13 No.1
Angiodysplasia of the small bowel is an uncommon disorder in Korea, and is often found to be the cause of unexplained gastrointestinal bleeding. A 53-year-old male patient visited an emergeney room because of repeated melena and dizziness, He underwent selective superior mesenteric arterial angiography which revealed abnormal clusters of small arteries and early opacification of veins draining a segment of lower jejunum. An exploratory laparotomy was performed under the impression of angiodysplasia. An ulceration on the lower jejunum was found, and was resected. Subsequent jejunojejunostomy was performed. Histology of the resected specimen showed an irregularly dilated vessels in mucosa and submucosa.
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