돼지의 혈청 progesterone 수준에 의한 임신진단에 의한 연구

김상근(S . K . Kim),김무강(M . K . Kim),이규승(K . S . Lee),서길웅(K . W . Seo),김창식(C . S . Park),박항균(H . K . Park) 한국축산학회 1989 한국축산학회지 Vol.31 No.1

The study was carried out to investigate the changes of hormone levels in blood serum during the estrous cycle and the accuracy of pregnancy diagnosis by estimation of plasma progesterone at 20 to 23 days after breeding in sows. The progesterone and estradiol-17 R from blood serum samples were assayed by radioimmunoassay method. The results of this study were summarized as follows: I.The progesterone concentrations in blood serum during the estrous cycle began to decline rapidly at 2 days before estrus, and thereafter decreased to 0.7±0.18ng/ml at on the day of estrus, and thereafter reached a peak mean level of 13.1±2.15ng/ml at 15 days after estrus. 2.The estradiol-17β concentrations in blood serum during the estrous cycle showed a peak mean level of 14.6±3.24pg/ml at 18 days after estrus, and decreased from 9.8 pg/ml to 14.2 pg/ml at luteal phase. 3.During 20 to 23 days after artificial insemination, the accuracy of pregnancy diagnosis from the blood serum progesterone levels were 96.4% for non-pregnant sows($lt;2.7ng/ml), and 86.1% for pregnant sows( 3.6ng/ml). The average overall accuracy of pregnancy prediction for non-pregnant and pregnant sows was 91.3%.

육경절단이 사슴뿔의 성장에 미치는 영향

김상우 ( S. W. Kim ),최순호 ( S. H. Choi ),상병돈 ( B. D. Sang ),김영근 ( Y. K. Kim ),이승수 ( S. S. Lee ),상병찬 ( B. C. Sang ),서길웅 ( K. W. Seo ) 한국동물자원과학회 2006 한국축산학회지 Vol.48 No.6

육경 조직판 이식이 사슴뿔의 성장에 미치는 영향

김상우 ( S. W. Kim ),최순호 ( S. H. Choi ),상병돈 ( B. D. Sang ),김영근 ( Y. K. Kim ),이승수 ( S. S. Lee ),상병찬 ( B. C. Sang ),서길웅 ( K. W. Seo ) 한국동물자원과학회 2006 한국축산학회지 Vol.48 No.6

한국재래산양정자 (韓國在來山羊精子) 의 액상 및 동결보존에 관한 연구

박창식,양문한,황덕수,이규승,서길웅 ( C . S . Park,M . H . Yang,D . S . Hwang,K . S . Lee,K . W . Seo ) 한국축산학회 1989 한국축산학회지 Vol.31 No.7

This study was carried out to develop the preservation methods of fresh and deep frozen Korean native goat spermatozoa and investigate the farrowing rate of Korean native goat does inseminated with frozen-thawed semen. The results obtained were as follow. 1. Motility and NAR acrosome of fresh spermatozoa during incubation were higher at 22℃ than at 5℃ or 37℃(p $lt;0.01). The percent LAC was inversely related to the percent NAR with regard to the incubation periods of these same three treatments. 2. Motility and NAR acrosome of spermatozoa diluted to 1:4 during incubation were higher at 22℃ than at 5℃ or 37℃. Especially incubation for 0.5, 3 and 6 h in the 5℃ had a marked detrimental effect on motility and NAR acrosome of spermatozoa. 3. Motility and NAR acrosome of spermatozoa during incubation were higher for samples diluted 1:1, 1:2, or 1:4 than for samples diluted 1:6(p $lt;0.01). 4. Motility and NAR acrosome of post-thaw spermatozoa were higher at freezing rate of 12℃/min or 24℃/min than at freezing rate of 1℃/min(p $lt;0.01). 5. Motility and NAR acrosome of post-thaw spermatozoa at freezing rate of 12℃/min were higher at 9% glycerol than at 6% or 3%(p $lt;0.01). 6. After thawing of frozen spermatozoa in a 37℃ water bath for 10 sec, incubation temperatures of 12℃, 22℃ or 37℃ for 2h did not show any differences on motility and NAR acrosome. 7. Farrowing rate of Korean native goat does inseminated twice with frozen-thawed semen was 25.0%. Mean kid weight at birth was l.9kg.

RAPD기법을 이용한 축우의 유전적 다형성과 유사도 분석

이상훈,서길웅,권일,성창근,김선균,상병찬 충남대학교 농업과학연구소 1999 농업과학연구 Vol.26 No.2

본 연구는 축우 품종의 유전적 다형성을 분석하여 분자유전자수준에서 축우의 품종간 판별과 유전적 유사성을 구명하고자 Holstein종과 한우, Charolais종 및 한우 교잡종의 혈액에서 DNA를 추출하여, PCR(prolymerase chain reaction)기법에 의한 RAPD(random amplified polymorphic DNAs)분석을 실시하고, 소 품종간의 유사도 지수를 추정한 후 UPGMA(unweighted pair-group method using average)방법으로 유사도를 추정하였으며 그 결과를 요약하면 다음과 같다. 1. 축우 품종들로부터 추출된 genomic DNA를 1.5% agarose gel에서 전기영동한 결과, 12.2kb이상의 단열밴드로 나타났으며, genomic DNA의 순도는 DNA 흡광도 A_(260)과 A_(280)의 비율로 측정한 결과 그 비율은 1.75~2.10이었다. 2. RAPD기법에 의한 축우 품종의 유전적 다형성을 분석한 결과, 품종판별을 위한 가장 유용한 random primer는 5'-GAC CGC TTG T-3'인 것으로 판명되었다. 3. Primer 부착 온도에 따른 band 양상에 있어서 33.0℃인 경유는 약 340bp에서 Holstein 종과 Charolais종에서는 band가 출현되었으나, 한우와 한우 교잡종에서는 밴드가 나타나지 않았으며, 37.5℃의 경우에는 340bp에서 Holstein종과 한우에서는 밴드가 나타났으나, Charolais종과 한우교잡종에서는 band가 출현되지 않아 이들 밴드의 유무가 소 품종판별을 위한 유용한 유전적 표지로서의 이용이 가능할 것으로 사료되었다. 4. 축우 품종의 유사도 지수에 있어서는 primer 부착온도를 33.0℃와 37.5℃을 종합하였을때 Holstein종과 한우간에는 0.666~0.777이었으며, Charolais종간에는 0.615~0.666이었고, 한우와 Charolais종간에는 0.400~0.461로 다소 낮은 수치를 보였으나, 한우와 한우 교잡종간에는 0.857~0.888로 대체로 높은 계수를 보였다. 5. 이상의 결과를 종합하여 보면 RAPD기법에 의하여 random primer 5'-GAC CGC TTG T-3'으로 축우 품종의 판별이 가능하며, UPGMA에 의한 축우 품종의 유사도는 Holstein종과 한우 및 Charolais종간 보다는 한우와 한우 교잡종 및 Charolais종간의 유전적 유사도가 높은 것으로 나타났다. This study was carried out to investigate the difference and genetic similarity at the level of molecular genetics. Genomic DNA was extracted from blood of Holstein, Korean cattle, Charolais, and hybrid between Korean cattle and charolais and RAPD(random amplified polymorphic DNAs) was analyzed by PCR(polymerase chain reaction). After genetic similarity value from different breeds are analyzed, genetic similarity was estimated by UPGMA(unweighted pair-group method using average). The results obtained from this study can be summarized as follows; 1. When genomic DNA which was extracted from different breeds was subjected to electrophoresis on 1.5% agarose gel, bigger than 12.2kb was appeared Ratio by absorbance of A_(260)/A_(280) was 1.75∼2.10, indicating that genomic DNA was quite pure for RAPD analysis. 2. Different band patterns by RAPD were appeared according to the breeds in cattle. The best primer used to distinguish Holstein from other breeds was 5’-GAC CGC TTG T-3’. 3. A 340bp fragment was amplified in 33.0℃ of annealing temperature for the Holstein and Charolais breeds, but any amplification was not occurred in this annealing temperature for Korean cattle and hybrid. In addition, a 340bp fragment was amplified in 37.5℃ of annealing temperature for the Holstein and Korean cattle, but any amplification was not occurred in this annealing temperature for Charolais and hybrid. For the reaction of PCR, 37.5℃ and 33.0℃ of annealing temperature was shown to be best for genetic marker identification from Holstein, Charolais, and hybrid between Korean cattle and Charolais. 4. When genetic similarity from different breeds are analyzed at the both temperature of 33.0℃ and 37.5℃, the genetic similarity value of Holstein and Korean cattle, Holstein and Charolais, Korean cattle and Charolais, and Korean cattle and hybrid were 0.666∼0.777, 0.615∼0.666, 0.400∼0.461 and 0.857∼0.888, respectively. 5. It could be concluded that different breeds are capable of distinguishing by RAPD used random primer 5’-GAC CGC TTG T-3’, genetic similarity from different breeds was appeared the higher genetic similarity value of Korean cattle and Charolais than that of Holstein between Korean cattle and Charolais by UPGMA.

DNA분석기법을 이용한 한국재래산양육의 판별

상병찬,이상훈,류승희,서길웅,한성욱,김선균 충남대학교 농업과학연구소 1999 농업과학연구 Vol.26 No.2

재래산양의 유전자원 보존과 유전적 개량을 위하여 재래산양과 수입산양의 genomic DNA의 유전적 다형을 분석하기 위하여 수행되었으며 재래산양과 수입산양의 유전적 판별 분석은 RAPD기법을 이용하였으며 공시품종은 재래산양 30두, 재래산양 교잡종 10두, 수입산양 10두를 이용하였다 재래 산양육과 수입 산양육의 판별을 위한 시료는 재래 산양육 10두와 수입 산양육 10두를 이용하였다. 이들로부터 얻어진 결과를 요약하면 다음과 같다. 1. 재래산양, 수입산양 및 재래산양 교잡종으로부터 추출된 genomic DNA는 전기영동에 의해 약 23kb 크기의 DNA을 얻을 수 있었으며 UV spectrophotometer를 이용하여 흡광도 A 260과 A280의 비율로 측정한 결과, 그 비율이 175~210의 범위로 순도는 비교적 양호한 결과를 얻었다. 2. 순수 재래산양의 유전자원의 보존을 위한 재래산양의 유전자 감식여부를 탐색하기 위하여 약 110 여종의 random primer를 이용한 RAPD 기법에 의하여 재래산양, 수입산양 및 교잡종의 다형성을 분석한 결과 random primer OPO-19(5'-CAA ACG TCG G-3')를 이용하였을 때 재래산양에서만 396bp에서 band 가 나타났으며 수입산양과 교잡종에서는 vand가 나타났으며 수입산양과 교잡종에서는 band가 나타나지 않았다. 3. 또한, 재래 산양육과 수입 산양육의 유전적인 차이를 구명하기 위한 RAPD 기법에 의한 genomic DNA의 다형성 분석에 있어서도 random primer OPO-19(5'-CAA ACG TCG G-3')를 사용하였을 때 396bp에서 재래 산양육에서는 band가 나타났지만, 수입 산양육에서는 band가 나타나지 않았다. This study was carried out to analyze the genetic polymorphisms of genomic DNA of blood and meat for conservation of the genetic resources and genetic improvement of Korean Native goat. The genetic identification between Korean Native goat and imported goat was examined using RAPD(random amplified polymorphisms DNAs) analysis with 30 Korean Native goat, 10 hybrid, 10 imported goat. 10 Korean native goat meat and 10 imported goat meat. The results obtained from this study were summarized as follows. 1. Genomic DNA from Korean native goat, hybrid and imported goat could be obtained above about 23kb size using 0.5% agarose gel electrophoresis and the ratio of optical density at 260nm to that at 280nm was between 1.7 and 2.0 using UV spectrophtometer instrument. 2. In the results of the gene identification between Korean Native goat and hybrid, and imported goat using RAPD methods with random primer of 110 kinds, only Korean native goat showed a specific band at about 369bp using a random primer OPO-19 (5’-CAA ACG TCG G-3’), but imported goat and hybrid not showed. 3 Also, in the results of the gene identification between Korean Native goat meat and imported goat meat using RAPD methods with random primer, Korean native goat only showed a specific band at about 369bp using a random primer No 19(5’-CAA ACG TCG G-3’). but imported goat not showed.

배양액 및 난구세포가 돼지난포란의 체외수정에 미치는 영향

박병권,한만희,서길웅,박창식,이규승 충남대학교 농업과학연구소 1996 농업과학연구 Vol.23 No.2

This study was conducted to investigate the effects of medium and cumulus cell on in vitro fertilization of porcine follicular oocytes. The results obtained are as follows ; 1. The normal fertilization rates of in vitro matured follicular oocytes coultured in BO, mTALP and TCM-HEPES medium were 14.0~24.3%, 30.8~32.7% and 21.4~23.9%, respectively. These data indicated that the optimal medium for fertilization on porcine oocytes in vitro was the mTALP medium. 2. The normal fertilization rates of epididymal sperm were 24.3%(BO), 30.8%(mTALP) and 23.9%(TCM-HEPES), and those of ejaculated sperm were 14.0%(BO), 32.7%(mTALP) and 21.4%(TCM-HEPES). 3. The sperm penetration rates of cumulus-enclosed and cumulus-free oocytes on in vitro feritilization were 54.0% and 72.0%. The normal fertilization rates of cumulus-enclosed and cumulus-free oocytes were 11.9% and 21.5%. The normal fertilization rate of cumulus-enclosed oocytes was significantly(P<0.05) higher than that of cumulus-free oocytes.